江淮地区不同品种光敏型高丹草对土壤微生态环境的影响
|
摘要
为了研究江淮地区不同品种光敏型高丹草(Sorghum-sudangrass hybrid)对土壤微生态环境的影响,本研究选取海牛Monster(HN)、帕卡Pa Kahuna(PK)、大卡Big Kahuna(DK)和高丹草BJ0603(GDC)4个品种,利用传统法测定不同土层土壤理化性质、使用试剂盒测定根际土壤酶活性,通过高通量测序法对根际土壤细菌群落多样性进行了分析。结果表明:各品种根部土壤理化性质、酶活性及微生物多样性具有显著的品种差异;PK根际土壤细菌多样性显著高于其他3个品种;根际土壤细菌群落多样性变化与土壤总氮(Total nitrogen,TN)及有机质(Organic matter,OM)含量变化关系较大。综上,不同品种土壤微生态环境差异显著,因此,根据不同品种特性制定引种及管理方案可以在提高产量和品质的基础上改良土壤,提高土地生产力。
The objective of this study was to investigate the effects of different cultivars of photosensitive Sorghum-sudangrass hybrid on soil microecological environment in Jianghuai region.Four Sorghum Sudangrass hybrid cultivars were selected in this study:Monster(HN),Big Kahuna(DK),BJ0603(GDC)and Pa Kahuna(PK).Traditional methods were used to determine the physical and chemical properties of soil in different soil layers,enzyme activities were determined by kits in rhizosphere soil,and the diversity of bacterial community in rhizosphere soil was analyzed by Illumina MiSeq analysis.Results showed that the soil physical chemical properties and enzyme activities were significantly different(P<0.05)in soil of four cultivars;The Illumina Miseq analysis indicated that bacterial diversities were different in soil of different cultivars;The soil bacterial diversity of PK was higher than the other three cultivars;The redundancy analyses(RDA)analysis indicated that soil total nitrogen(TN)and soil organic matter(OM)were the most important factors to characterize the soil bacterial community composition.In summary,the cultivars of HN,PK,DK and GDC in Jiang-huai region showed cultivar differences in soil nutrient,bulk density,enzyme activity and bacterial community structure diversity,so different planting management schemes could be formulated according to the characteristics of different cultivars.
The objective of this study was to investigate the effects of different cultivars of photosensitive Sorghum-sudangrass hybrid on soil microecological environment in Jianghuai region.Four Sorghum Sudangrass hybrid cultivars were selected in this study:Monster(HN),Big Kahuna(DK),BJ0603(GDC)and Pa Kahuna(PK).Traditional methods were used to determine the physical and chemical properties of soil in different soil layers,enzyme activities were determined by kits in rhizosphere soil,and the diversity of bacterial community in rhizosphere soil was analyzed by Illumina MiSeq analysis.Results showed that the soil physical chemical properties and enzyme activities were significantly different(P<0.05)in soil of four cultivars;The Illumina Miseq analysis indicated that bacterial diversities were different in soil of different cultivars;The soil bacterial diversity of PK was higher than the other three cultivars;The redundancy analyses(RDA)analysis indicated that soil total nitrogen(TN)and soil organic matter(OM)were the most important factors to characterize the soil bacterial community composition.In summary,the cultivars of HN,PK,DK and GDC in Jiang-huai region showed cultivar differences in soil nutrient,bulk density,enzyme activity and bacterial community structure diversity,so different planting management schemes could be formulated according to the characteristics of different cultivars.
引文
[1]蒋婧,宋明华.植物与土壤微生物在调控生态系统养分循环中的作用[J].植物生态学报,2010,34(8):979-988
[2]Li L,Liang X,Ye Y,et al.Effects of repeated swine manure applications on legacy phosphorus and phosphomonoesterase activities in a paddy soil[J].Biology&Fertility of Soils,2015,51(2):167-181
[3]Lupwayi N Z,Rice W A,Clayton G W.Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation[J].Soil Biology&Biochemistry,1998,30(13):1733-1741
[4]Guo Z Y,Kong C H,Wang J G,et al.Rhizosphere isoflavones(daidzein and genistein)levels and their relation to the microbial community structure of mono-cropped soybean soil in field and controlled conditions[J].Soil Biology&Biochemistry,2011,43(11):2257-2264
[5]Rietz D N,Haynes R J.Effects of irrigation-induced salinity and sodicity on soil microbial activity[J].Soil Biology&Biochemistry,2003,35(6):845-854
[6]Garbeva P,van Veen J A,van Elsas J D.Microbail diversity in soil:selection of microbial populations by plant and soil type and implications for disease suppressiveness[J].Annual Review of Phytopathology,2004,42(1):243-270
[7]俞琳飞,李廷轩,王晟强,等.植茶品种对土壤动物群落结构的影响[J].生态学杂志,2018,37(4):1220-1226
[8]钱秋平,杨统一,程林润,等.不同大豆品种对根际土壤微生物群落影响的差异[J].南京林业大学学报:自然科学版,2010,34(5):1-6
[9]李勇,黄小芳,丁万隆.根系分泌物及其对植物根际土壤微生态环境的影响[J].华北农学报,2008,23(S1):182-186
[10]梁儒彪,梁进,乔明锋,等.模拟根系分泌物C:N化学计量特征对川西亚高山森林土壤碳动态和微生物群落结构的影响[J].植物生态学报,2015,39(5):466-476
[11]杨统一,曲静怡,赵卫国,等.不同抗病性桑树品种根际土壤微生态环境特征分析[J].蚕业科学,2015,41(2):211-217
[12]何振富,贺春贵,王国栋,等.种植密度对陇东旱塬区光敏型高丹草生物学性状及产量的影响[J].草业科学,2018,35(5):1188-1198
[13]陈立新.土壤实验实习教程[M].哈尔滨:东北林业大学出版社,2005:59-63
[14]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000:106-108
[15]Chao A.Stopping rules and estimation for recapture debugging with unequal failure rates[J].Biometrika,1993,80(1):193-201
[16]Chao A.Nonparametric estimation of the number of classes in apopulation[J].Scandinavian Journal of Statistics,1984,11(4):265-270
[17]Shannon C E.A mathematical theory of communication[J].Bell Systems Technical Journal,1948,27(4):623-656
[18]魏强,王芳,陈文业,等.黄河上游玛曲不同退化程度高寒草地土壤物理特性研究[J].水土保持通报,2010,30(5):16-21
[19]付春娜.生物炭对不同马铃薯品种生长及产量的影响[D].哈尔滨:东北农业大学,2016:24-30
[20]伏兵哲,高雪芹,蔡伟,等.宁夏引黄灌区种植不同苜蓿品种对土壤速效养分的影响[J].中国草地学报,2018,40(2):20-26
[21]丁莎莎.不同苎麻品种根际营养及根际微生物的比较研究[D].长沙:湖南农业大学,2012:20-28
[22]杨安,曾艳,汪婷,等.川西茶区4个主栽茶树品种生化性质和适制性的研究[J].西南农业学报,2013,26(1):119-124
[23]Zhou X,Yu G,Wua F.Effects of intercropping cucumber with onion or garlic on soil enzyme activities,microbial communities and cucumber yield[J].European Journal of Soil Biology,2011,47(5):279-287
[24]杨姝,贾乐,毕玉芬,等.7种紫花苜蓿对云南某铅锌矿区土壤镉铅的累积特征及品种差异[J].农业资源与环境学报,2018,35(3):38-44
[25]刘落鱼,佘汉基,周彤彤,等.10种茶花的土壤特性研究[J].经济林研究,2018,36(6):139-145
[26]Acostamartínez V,Burow G,Zobeck TM,et al.Soil Microbial Communities and Function in Alternative Systems to Continuous Cotton[J].Soil Science Society of America Journal,2010,74(4):1181-1192
[27]Dick R P,Pankhurst C,Doube B M,et al.Soil enzyme activities as integrative indicators of soil health[M].Wallingford,UK:CAB international,1997:121-156
[28]Little K R,Rose M T,Jackson W R,et al.Do lignite-derived organic amendments improve early-stage pasture growth and key soil biological and physicochemical properties?[J].Crop&Pasture Science,2014(9),65:899
[29]生静雅,朱海军,刘广勤,等.不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究[J].生态与农村环境学报,2017,33(9):816-821
[30]张立成,彭沛宇,廖健程,等.稻-稻-油菜轮作土壤细菌群落的特征[J].应用与环境生物学报,2018,24(2):1-9
[31]王光华,刘俊杰,于镇华,等.土壤酸杆菌门细菌生态学研究进展[J].生物技术通报,2016,32(2):14-20
[32]Vasconcellos R L F D,Ribeiro C M.Isolation and screening for plant growth-promoting(PGP)actinobacteria from Araucaria angustifolia rhizosphere soil[J].Scientia Agricola,2010,67(6):743-746
[33]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772
[34]Fang C,Radosevich M,Fuhrmann J J.Characterization of rhizosphere microbial community structure in five similar grass species using FAME and BIOLOG analyses[J].Soil Biology&Biochemistry,2001,33(4):679-682
[35]张英,芦光新,谢永丽,等.溶磷菌分泌有机酸与溶磷能力相关性研究[J].草地学报,2015,23(5):1033-1038
[36]邬嘉华,王立新,张景慧,等.温带典型草原土壤理化性质及微生物对放牧强度的响应[J].草地学报,2018,26(7):832-840
[37]Davis J R,Huisman O C,Everson D O,et al.Verticillium wilt of potato:a model of key factors related to disease severity and tuber yield in Southeastern Idaho[J].American Journal of Potato Research,2001,78(4):291-300
[2]Li L,Liang X,Ye Y,et al.Effects of repeated swine manure applications on legacy phosphorus and phosphomonoesterase activities in a paddy soil[J].Biology&Fertility of Soils,2015,51(2):167-181
[3]Lupwayi N Z,Rice W A,Clayton G W.Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation[J].Soil Biology&Biochemistry,1998,30(13):1733-1741
[4]Guo Z Y,Kong C H,Wang J G,et al.Rhizosphere isoflavones(daidzein and genistein)levels and their relation to the microbial community structure of mono-cropped soybean soil in field and controlled conditions[J].Soil Biology&Biochemistry,2011,43(11):2257-2264
[5]Rietz D N,Haynes R J.Effects of irrigation-induced salinity and sodicity on soil microbial activity[J].Soil Biology&Biochemistry,2003,35(6):845-854
[6]Garbeva P,van Veen J A,van Elsas J D.Microbail diversity in soil:selection of microbial populations by plant and soil type and implications for disease suppressiveness[J].Annual Review of Phytopathology,2004,42(1):243-270
[7]俞琳飞,李廷轩,王晟强,等.植茶品种对土壤动物群落结构的影响[J].生态学杂志,2018,37(4):1220-1226
[8]钱秋平,杨统一,程林润,等.不同大豆品种对根际土壤微生物群落影响的差异[J].南京林业大学学报:自然科学版,2010,34(5):1-6
[9]李勇,黄小芳,丁万隆.根系分泌物及其对植物根际土壤微生态环境的影响[J].华北农学报,2008,23(S1):182-186
[10]梁儒彪,梁进,乔明锋,等.模拟根系分泌物C:N化学计量特征对川西亚高山森林土壤碳动态和微生物群落结构的影响[J].植物生态学报,2015,39(5):466-476
[11]杨统一,曲静怡,赵卫国,等.不同抗病性桑树品种根际土壤微生态环境特征分析[J].蚕业科学,2015,41(2):211-217
[12]何振富,贺春贵,王国栋,等.种植密度对陇东旱塬区光敏型高丹草生物学性状及产量的影响[J].草业科学,2018,35(5):1188-1198
[13]陈立新.土壤实验实习教程[M].哈尔滨:东北林业大学出版社,2005:59-63
[14]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000:106-108
[15]Chao A.Stopping rules and estimation for recapture debugging with unequal failure rates[J].Biometrika,1993,80(1):193-201
[16]Chao A.Nonparametric estimation of the number of classes in apopulation[J].Scandinavian Journal of Statistics,1984,11(4):265-270
[17]Shannon C E.A mathematical theory of communication[J].Bell Systems Technical Journal,1948,27(4):623-656
[18]魏强,王芳,陈文业,等.黄河上游玛曲不同退化程度高寒草地土壤物理特性研究[J].水土保持通报,2010,30(5):16-21
[19]付春娜.生物炭对不同马铃薯品种生长及产量的影响[D].哈尔滨:东北农业大学,2016:24-30
[20]伏兵哲,高雪芹,蔡伟,等.宁夏引黄灌区种植不同苜蓿品种对土壤速效养分的影响[J].中国草地学报,2018,40(2):20-26
[21]丁莎莎.不同苎麻品种根际营养及根际微生物的比较研究[D].长沙:湖南农业大学,2012:20-28
[22]杨安,曾艳,汪婷,等.川西茶区4个主栽茶树品种生化性质和适制性的研究[J].西南农业学报,2013,26(1):119-124
[23]Zhou X,Yu G,Wua F.Effects of intercropping cucumber with onion or garlic on soil enzyme activities,microbial communities and cucumber yield[J].European Journal of Soil Biology,2011,47(5):279-287
[24]杨姝,贾乐,毕玉芬,等.7种紫花苜蓿对云南某铅锌矿区土壤镉铅的累积特征及品种差异[J].农业资源与环境学报,2018,35(3):38-44
[25]刘落鱼,佘汉基,周彤彤,等.10种茶花的土壤特性研究[J].经济林研究,2018,36(6):139-145
[26]Acostamartínez V,Burow G,Zobeck TM,et al.Soil Microbial Communities and Function in Alternative Systems to Continuous Cotton[J].Soil Science Society of America Journal,2010,74(4):1181-1192
[27]Dick R P,Pankhurst C,Doube B M,et al.Soil enzyme activities as integrative indicators of soil health[M].Wallingford,UK:CAB international,1997:121-156
[28]Little K R,Rose M T,Jackson W R,et al.Do lignite-derived organic amendments improve early-stage pasture growth and key soil biological and physicochemical properties?[J].Crop&Pasture Science,2014(9),65:899
[29]生静雅,朱海军,刘广勤,等.不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究[J].生态与农村环境学报,2017,33(9):816-821
[30]张立成,彭沛宇,廖健程,等.稻-稻-油菜轮作土壤细菌群落的特征[J].应用与环境生物学报,2018,24(2):1-9
[31]王光华,刘俊杰,于镇华,等.土壤酸杆菌门细菌生态学研究进展[J].生物技术通报,2016,32(2):14-20
[32]Vasconcellos R L F D,Ribeiro C M.Isolation and screening for plant growth-promoting(PGP)actinobacteria from Araucaria angustifolia rhizosphere soil[J].Scientia Agricola,2010,67(6):743-746
[33]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772
[34]Fang C,Radosevich M,Fuhrmann J J.Characterization of rhizosphere microbial community structure in five similar grass species using FAME and BIOLOG analyses[J].Soil Biology&Biochemistry,2001,33(4):679-682
[35]张英,芦光新,谢永丽,等.溶磷菌分泌有机酸与溶磷能力相关性研究[J].草地学报,2015,23(5):1033-1038
[36]邬嘉华,王立新,张景慧,等.温带典型草原土壤理化性质及微生物对放牧强度的响应[J].草地学报,2018,26(7):832-840
[37]Davis J R,Huisman O C,Everson D O,et al.Verticillium wilt of potato:a model of key factors related to disease severity and tuber yield in Southeastern Idaho[J].American Journal of Potato Research,2001,78(4):291-300