口腔癌患者手术前后T细胞亚群、T细胞内细胞因子及免疫球蛋白的检测分析
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摘要
目的:探讨口腔癌患者手术前后T细胞亚群、T细胞内细胞因子及免疫球蛋白的水平变化。方法:选取2017年1~12月我院收治的40例口腔癌患者作为观察组,选取同期于我院体检的40例健康志愿者作为对照组,检测观察组手术前后T细胞亚群、T细胞内细胞因子及免疫球蛋白的水平变化,并与对照组做比较分析。结果:观察组手术前CD3~+、CD4~+、CD4~+/CD8~+低于对照组,CD8~+高于对照组,差异均有统计学意义(P<0.05);观察组手术后CD3~+、CD4~+、CD4~+/CD8~+分别为(64.98±3.88)%、(42.40±3.53)%、(1.56±0.41),均较手术前升高(P<0.05),但仍低于对照组(P<0.05);观察组手术后CD8~+为(27.25±3.54)%,较手术前降低(P<0.05),但仍高于对照组(P<0.05)。观察组手术前INF-γ~+、CD4~+/INF-γ~+、IL-2~+、CD4~+/IL-2~+均低于对照组,差异均有统计学意义(P<0.05);观察组手术后INF-γ~+、CD4~+/INF-γ~+、IL-2~+、CD4~+/IL-2~+分别为(0.15±0.06)%、(0.07±0.03)%、(0.47±0.13)%、(0.16±0.11)%,均较手术前升高(P<0.05),但仍低于对照组(P<0.05)。观察组手术前、手术后IgA、IgG分别为(2.01±0.13)、(1.81±0.12)、(10.53±3.12)、(8.41±2.01)g/L,均较对照组升高,差异有统计学意义(P<0.05);观察组手术前后IgA、IgG、IgM均无显著变化(P>0.05)。结论:手术可有效改善口腔癌患者T淋巴细胞亚群及T细胞内细胞因子水平,对提高免疫功能具有积极的促进作用。
Objective:To investigate the changes of T cell subsets,T cell cytokine and immunoglobulin in patients with oral cancer before and after operation.Methods:40 cases of oral cancer treated in our hospital from January 2017 to December2017 were selected as the observation group,and 40 healthy volunteers in our hospital were selected as the control group.The levels of T cell subsets,T cell cytokine and immunoglobulin in the observation group before and after operation were detected and compared with those in the control group.Results:The CD3~+,CD4~+and CD4~+/CD8~+of the observation group before operation were lower than those of the control group,and CD8~+was higher than that of the control group,the differences were statistically significant(P<0.05).The CD3~+,CD4~+and CD4~+/CD8~+after operation in the observation group were(64.98±3.88)%,(42.40±3.53)%and(1.56±0.41)respectively,which were all higher than those before the operation(P<0.05),but still lower than those in the control group(P<0.05).The CD8~+of the observation group was(27.25 ± 3.54)% lower than that before operation(P<0.05),but it was still higher than that of the control group(P<0.05).In the observation group,the levels of INF-γ~+,CD4~+/INF-γ~+,IL-2~+,and CD4~+/IL-2~+before operation were lower in the observation group than those in the control group(P<0.05).In the observation group,INF-γ~+,CD4~+/INF-γ~+,IL-2~+,and CD4~+/IL-2~+after surgery were(0.15±0.06)%,(0.07±0.03)%,(0.47±0.13)%,(0.16 ± 0.11)%,were higher than those before surgery(P<0.05),but still lower than those in the control group(P<0.05).The IgA and IgG before and after the surgery in the observation group were(2.01±0.13)g/L,(1.81±0.12)g/L,(10.53±3.12)g/L,and(8.41±2.01)g/L,respectively,compared with the control group,the difference was statistically significant(P<0.05).There was no significant change in IgA,IgG and IgM in the observation group before and after operation(P>0.05).Conclusion:Surgery can effectively improve the T lymphocyte subsets and T cell cytokine levels in oral cancer patients,and has a positive effect on improving immune function.
Objective:To investigate the changes of T cell subsets,T cell cytokine and immunoglobulin in patients with oral cancer before and after operation.Methods:40 cases of oral cancer treated in our hospital from January 2017 to December2017 were selected as the observation group,and 40 healthy volunteers in our hospital were selected as the control group.The levels of T cell subsets,T cell cytokine and immunoglobulin in the observation group before and after operation were detected and compared with those in the control group.Results:The CD3~+,CD4~+and CD4~+/CD8~+of the observation group before operation were lower than those of the control group,and CD8~+was higher than that of the control group,the differences were statistically significant(P<0.05).The CD3~+,CD4~+and CD4~+/CD8~+after operation in the observation group were(64.98±3.88)%,(42.40±3.53)%and(1.56±0.41)respectively,which were all higher than those before the operation(P<0.05),but still lower than those in the control group(P<0.05).The CD8~+of the observation group was(27.25 ± 3.54)% lower than that before operation(P<0.05),but it was still higher than that of the control group(P<0.05).In the observation group,the levels of INF-γ~+,CD4~+/INF-γ~+,IL-2~+,and CD4~+/IL-2~+before operation were lower in the observation group than those in the control group(P<0.05).In the observation group,INF-γ~+,CD4~+/INF-γ~+,IL-2~+,and CD4~+/IL-2~+after surgery were(0.15±0.06)%,(0.07±0.03)%,(0.47±0.13)%,(0.16 ± 0.11)%,were higher than those before surgery(P<0.05),but still lower than those in the control group(P<0.05).The IgA and IgG before and after the surgery in the observation group were(2.01±0.13)g/L,(1.81±0.12)g/L,(10.53±3.12)g/L,and(8.41±2.01)g/L,respectively,compared with the control group,the difference was statistically significant(P<0.05).There was no significant change in IgA,IgG and IgM in the observation group before and after operation(P>0.05).Conclusion:Surgery can effectively improve the T lymphocyte subsets and T cell cytokine levels in oral cancer patients,and has a positive effect on improving immune function.
引文
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14 Kalavrezos N,Scully C.Mouth cancer for clinicians.part 9:the patient and care team[J].Dent Update,2016,43(3):276-278,281-282,285-287.
15 张健,朱赟,王诚丽.口腔癌患者外周血免疫变化与临床结局关系研究[J].现代医院,2016,16(8):1175-1177,1180.
16 Ferrer-Orta C,de la Higuera I,Caridi F,et al.Multifunctionality of a picornavirus polymerase domain:nuclear localization signal and nucleotide recognition[J].J Virol,2015,89(13):6848-6859.
17 Qasaimeh G R,Matalqah I,Bakkar S,et al.Xanthogranulomatous cholecystitis in the laparoscopic era is still a challenging disease[J].J Gastrointest Surg,2015,19(6):1036-1042.
18 Zeng J,Liu R,Wang J,et al.A bispecific antibody directly induces lymphoma cell death by simultaneously targeting CD20and HLA-DR[J].J Cancer Res Clin Oncol,2015,141(11):1899-1907.
19 Wu X,Qi D,Shen X,et al.The molecular pathology detection analysis of Ig/TCR gene rearrangement in malignant lymphoma diagnosis[J].Guizhou MedicalJournal,2016,1(9):908-910.
20 潘海兴.口腔癌患者手术前后免疫球蛋白和补体检测及其临床意义[D].中南大学,2008.
2 李天客,包阳,陈中,等.口腔癌患者手术前后细胞免疫功能的变化及临床意义[J].中国老年学杂志,2018,38(4):827-829.
3 符良斌,廖天安,王鸿,等.口腔癌患者术后短期细胞免疫功能的变化[J].西南国防医药,2015,25(8):865-867.
4 粟晓玲,马金辉,萧福元,等.嚼槟榔与口腔癌关系的病例对照研究[J].实用预防医学,2016,23(9):1059-1062.
5 Yu T,Guo P,Wu Y,et al.The role of chemotherapy and operation on lymphocytes accumulation in peripheral blood obtained from patients with oral squamous cell carcinoma[J].Springerplus,2015,4:698.
6 Uchiyama K,Yamada M,Komatsu S,et al.Determination of the appropriate timing of aprepitant administration for nausea in patients with head and neck cancer receiving combination chemotherapy with docetaxel,nedaplatin divided doses for 5Days),and 5-fluorouracil[J].Gan To Kagaku Ryoho,2015,42(10):1191-1195.
7 Siegel RL,Miller KD,Jemal A.Cancer statistics,2018[J].CA Cancer J Clin,2018,68(1):7-30.
8 Ren JG,Zhang W,Liu B,et al.Clinical significance and roles in angiogenesis of circulating microparticles in oral cancer[J].J Dent Res,2016,95(8):860-867.
9 Kalavrezos N,Scully C.Mouth cancer for clinicians.part 8:referral[J].Dent Update,2016,43(2):176-178,181-182,184-185.
10 Massaro A N,Chang T,Baumgart S,et al.Biomarkers S100B and neuron-specific enolase predict outcome in hypothermiatreated encephalopathic newborns[J].Pediatr Crit Care Med,2014,15(7):615-622.
11 Youssef MM,Paish EC,Murray JC,et al.Tumor infiltrating t lymphocytes and apoptosis in colorectal cancer[J].Egypt J Immunol,2015,22(1):19-28.
12 Lohneis P,Boral S,Kaufmann AM,et al.Human papilloma virus status of penile squamous cell carcinoma is associated with differences in tumour-infiltrating T lymphocytes[J].Virchows Arch,2015,466(3):323-331.
13 Coyne B.Mouth Cancer for Clinicians Part 7(Dent Update2016;50-65)[J].Dent Update,2016,43(2):193.
14 Kalavrezos N,Scully C.Mouth cancer for clinicians.part 9:the patient and care team[J].Dent Update,2016,43(3):276-278,281-282,285-287.
15 张健,朱赟,王诚丽.口腔癌患者外周血免疫变化与临床结局关系研究[J].现代医院,2016,16(8):1175-1177,1180.
16 Ferrer-Orta C,de la Higuera I,Caridi F,et al.Multifunctionality of a picornavirus polymerase domain:nuclear localization signal and nucleotide recognition[J].J Virol,2015,89(13):6848-6859.
17 Qasaimeh G R,Matalqah I,Bakkar S,et al.Xanthogranulomatous cholecystitis in the laparoscopic era is still a challenging disease[J].J Gastrointest Surg,2015,19(6):1036-1042.
18 Zeng J,Liu R,Wang J,et al.A bispecific antibody directly induces lymphoma cell death by simultaneously targeting CD20and HLA-DR[J].J Cancer Res Clin Oncol,2015,141(11):1899-1907.
19 Wu X,Qi D,Shen X,et al.The molecular pathology detection analysis of Ig/TCR gene rearrangement in malignant lymphoma diagnosis[J].Guizhou MedicalJournal,2016,1(9):908-910.
20 潘海兴.口腔癌患者手术前后免疫球蛋白和补体检测及其临床意义[D].中南大学,2008.