HPLC法测定金银清瘟合剂中有效成分含量
|
摘要
目的:建立金银清瘟合剂中君药金银花主成分绿原酸含量测定方法。方法:采用水煎提取和蒸馏浓缩法提取处方中各药材有效成分制成合剂,采用HPLC法测定:Diamonsil G8 ODS色谱柱(4.6 nm×200 mm,5μm);以50%甲醇-0.4%磷酸水溶液为流动相,流速1.0 mL·min~(-1),柱温30℃,检测波长327 nm,进样量10μL。结果:绿原酸保留时间为18.126 min,线性范围为0.991～9.93(r=0.999 9),平均加样回收率为99.16%,检测限为0.1μg·mL~(-1)。结论:金银清瘟合剂制备工艺稳定,HPLC法测定绿原酸含量高效、准确、稳定,质量标准可行。
Objective:To establish a method for the determination of chlorogenic acid content in jinyin qingwen mixture. Methods: Water decoction and distillation were used to extract the effective components of the medicinal materials. HPLC method was used to determine the content of chlorogenic acid, Use Diamonsil G8 ODS column(4.6 nm×200 mm, 5 μm), taking 50% methanol-0.4% phosphoric acid water as mobile phase,the flow rate was 1.0 mL·min~(-1), column temperature was 30 ℃, detection wave length was 327 nm, sample quantity was 10 μL. Results: Chlorogenic acid retention time was 18.126 min, linear range was 0.991-9.93(r=0.999 9), average recovery was 99.16%, detection limit was 0.1 μg·mL~(-1). Conclusion: The preparation process of jinyin qingwen mixture is stable.HPLC method for determination of chlorogenic acid content is efficient, accurate and stable, and the quality standard is feasible.
Objective:To establish a method for the determination of chlorogenic acid content in jinyin qingwen mixture. Methods: Water decoction and distillation were used to extract the effective components of the medicinal materials. HPLC method was used to determine the content of chlorogenic acid, Use Diamonsil G8 ODS column(4.6 nm×200 mm, 5 μm), taking 50% methanol-0.4% phosphoric acid water as mobile phase,the flow rate was 1.0 mL·min~(-1), column temperature was 30 ℃, detection wave length was 327 nm, sample quantity was 10 μL. Results: Chlorogenic acid retention time was 18.126 min, linear range was 0.991-9.93(r=0.999 9), average recovery was 99.16%, detection limit was 0.1 μg·mL~(-1). Conclusion: The preparation process of jinyin qingwen mixture is stable.HPLC method for determination of chlorogenic acid content is efficient, accurate and stable, and the quality standard is feasible.
引文
[1] 刘玉婕,王长福,齐彦,等.金银花,连翘及其配伍后对临床11种致病菌的作用研究[J].中医药学报,2016,44(5):43-46.
[2] 康正,刘琳,程春生.浅谈中药抗菌作用的研究进展[J].世界最新医学信息文摘,2015,15(99):45.
[3] Rodvold KA,Maonoghy KW.Methicillin-resistant Staphylococcus aureus therapy:past,present,and future[J].Clin Infect Dis,2014,58(Suppl 1):S20-S27.
[4] 刘玉婕,王长福,齐彦,等.金银花,连翘及其配伍后对临床11种致病菌的作用研究[J].中医药学报,2016,33(4):14-16.
[5] 叶小华,傅锦坚,周俊立,等.儿童携带耐甲氧西林金黄色葡萄球菌及影响因素[J].中国妇幼保健,2014,29(9):1359-1361.
[6] 张银维,周华,蔡洪流,等.鲍曼不动杆菌血流感染临床特征和死亡危险因素分析[J].中华内科杂志,2016,55(2):121-126.
[7] 冯欣煜,姚志凌,李明辉.金银花的现代药理和临床运用研究进展[J].中医研究,2015,28(9):78-82.
[8] 程敏,梁芷韵,张文莉,等.金银花提取物的质量评价研究[J].广州中医药大学学报,2018,35(3):511-518.
[9] 梁芸,南蓬.金银花提取物在乳液化妆品中的抑菌活性及应用[J].广东化工,2016,43(23):1.
[10] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:30-31,221,1108-1110,1146-1157,1495-1489.
[11] 艾光丽,曾桢,王维,等.金银花提取物的质量分析方法评价[J].中国实验方剂学杂志,2017,23(5):22.
[12] 李丹,李会军,高雯,等.一测多评法测定金银化提取物中6种绿原酸类成分[J].中国中药杂志,2014,39(12):2305.
[13] 徐莹,许剑峰.金银花提取物的质量管制研究[J].黑龙江科技信息,2013(14):16.
[14] 叶思勇,陈智.正交试验法优选金银花总黄酮的提取工艺[J].国际中医中药杂志,2017,39(9):820-823.
[15] 郑佳盛,王守箐.金银花罐组式逆流提取工艺的估化[J].中成药,2017:2193-2195.
[16] 王赛君,任振峰,杨明,等.中药提取技术及其在国内的转化应用研究[J].中国中药杂志,2014,39(8):1360-1366.
[17] 杨彬,罗磊,朱文学,等.金银花绿原酸和木犀草苷的提取[J].食品与发酵工业学,2015,41(3):225-232.
[18] 曾雪,杨元娟,陈竹,等.HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量[J].中国药房,2017,28(18):2543-2545.
[19] 梁德东,马钦海,宋帅,等.HPLC法同时测定银翘马勃散中5种有效成分的含量[J].中药新药与临床药理,2017,28(1):84-88.
[20] 欧水平,张文志,陈灵.金银花与山银花抗病毒酚酸类和黄酮类成分的差异性研究[J].中国药房,2015,26(33):4750-4752.
[2] 康正,刘琳,程春生.浅谈中药抗菌作用的研究进展[J].世界最新医学信息文摘,2015,15(99):45.
[3] Rodvold KA,Maonoghy KW.Methicillin-resistant Staphylococcus aureus therapy:past,present,and future[J].Clin Infect Dis,2014,58(Suppl 1):S20-S27.
[4] 刘玉婕,王长福,齐彦,等.金银花,连翘及其配伍后对临床11种致病菌的作用研究[J].中医药学报,2016,33(4):14-16.
[5] 叶小华,傅锦坚,周俊立,等.儿童携带耐甲氧西林金黄色葡萄球菌及影响因素[J].中国妇幼保健,2014,29(9):1359-1361.
[6] 张银维,周华,蔡洪流,等.鲍曼不动杆菌血流感染临床特征和死亡危险因素分析[J].中华内科杂志,2016,55(2):121-126.
[7] 冯欣煜,姚志凌,李明辉.金银花的现代药理和临床运用研究进展[J].中医研究,2015,28(9):78-82.
[8] 程敏,梁芷韵,张文莉,等.金银花提取物的质量评价研究[J].广州中医药大学学报,2018,35(3):511-518.
[9] 梁芸,南蓬.金银花提取物在乳液化妆品中的抑菌活性及应用[J].广东化工,2016,43(23):1.
[10] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:30-31,221,1108-1110,1146-1157,1495-1489.
[11] 艾光丽,曾桢,王维,等.金银花提取物的质量分析方法评价[J].中国实验方剂学杂志,2017,23(5):22.
[12] 李丹,李会军,高雯,等.一测多评法测定金银化提取物中6种绿原酸类成分[J].中国中药杂志,2014,39(12):2305.
[13] 徐莹,许剑峰.金银花提取物的质量管制研究[J].黑龙江科技信息,2013(14):16.
[14] 叶思勇,陈智.正交试验法优选金银花总黄酮的提取工艺[J].国际中医中药杂志,2017,39(9):820-823.
[15] 郑佳盛,王守箐.金银花罐组式逆流提取工艺的估化[J].中成药,2017:2193-2195.
[16] 王赛君,任振峰,杨明,等.中药提取技术及其在国内的转化应用研究[J].中国中药杂志,2014,39(8):1360-1366.
[17] 杨彬,罗磊,朱文学,等.金银花绿原酸和木犀草苷的提取[J].食品与发酵工业学,2015,41(3):225-232.
[18] 曾雪,杨元娟,陈竹,等.HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量[J].中国药房,2017,28(18):2543-2545.
[19] 梁德东,马钦海,宋帅,等.HPLC法同时测定银翘马勃散中5种有效成分的含量[J].中药新药与临床药理,2017,28(1):84-88.
[20] 欧水平,张文志,陈灵.金银花与山银花抗病毒酚酸类和黄酮类成分的差异性研究[J].中国药房,2015,26(33):4750-4752.