细菌耐药性产生的分子机制及对细菌毒力的影响研究进展
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摘要
抗菌药物选择压力导致细菌耐药性日趋严重,一些细菌菌株的分子耐药机制会引起细菌致病性的改变,说明两者之间存在一定的相关性。论文从细菌胞壁、胞膜、胞质和染色体4个部分概述了其相关主要大分子物质参与细菌耐药的机制,介绍了与耐药性密切相关的生物大分子参与细菌致病的过程,分析细菌耐药性的产生对细菌毒力变化的影响,以期为解决现今较严重的细菌耐药性及细菌性疾病的防治难题找到新方法和突破点。
The phenomenon of bacterial drug resistance has become serious gradually because of the selective pressure of antimicrobials.Studies have shown that some molecular mechanisms of bacterial resistance could cause the change of bacterial pathogenicity,which indicated the relativities between the two.In this paper,we summarized the molecular mechanisms of bacterial resistance,introduced the bacterial pathogenic process which includes the molecules closely related to drug resistance,analyzed the effects of bacterial resistance on the changes of bacterial virulence from 4parts containing the bacterial cell wall,membrane,cytoplasm,and chromosome.We hope to find some new methods or breakthrough points to solve the difficult problems of the present serious bacterial resistance and bacterial disease prevention.
The phenomenon of bacterial drug resistance has become serious gradually because of the selective pressure of antimicrobials.Studies have shown that some molecular mechanisms of bacterial resistance could cause the change of bacterial pathogenicity,which indicated the relativities between the two.In this paper,we summarized the molecular mechanisms of bacterial resistance,introduced the bacterial pathogenic process which includes the molecules closely related to drug resistance,analyzed the effects of bacterial resistance on the changes of bacterial virulence from 4parts containing the bacterial cell wall,membrane,cytoplasm,and chromosome.We hope to find some new methods or breakthrough points to solve the difficult problems of the present serious bacterial resistance and bacterial disease prevention.
引文
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[25]刘珍,阙凤霞,李嫄渊,等.寒沙门菌质粒pRST98对细菌生物膜形成能力及毒力的影响[J].检验医学2015,30(10):1033-1038.
[26]Gupta P,Sothiselvam S,Vázquez-Laslop N,et al.Deregulation of translation due to post-transcriptional modification of rRNA explains why erm genes are inducible[J].Nat Commun,2013,4:1984.
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[28]李军,陈桂林.产ESBLs大肠埃希菌和肺炎克雷伯菌的耐药性分析及分布[J].河北医药,2016,18(38):2857-2891.
[29]刘泽世,呼瑞,张毅,等.非多重耐药及多重耐药鲍曼不动杆菌外排泵及其表达的研究[DB/OL].http://www.cnki.net/kcms/detail/61.1399.R.20161010.1814.014.
[30]Smani Y,López-Rojas R,Domínguez-Herrera J,et al.In vitro and in vivo reduced fitness and virulence in ciprofloxacin-resistant Acinetobacter baumannii[J].Clin Microbiol Infect,2012,18(1):E1-4.
[31]Tran Q T,Nawaz M S,Deck J,et al.Detection of type III secretion system virulence and mutations in gyrA and parC genes among quinolone-resistant strains of Pseudomonas aeruginosa isolated from imported shrimp[J].Foodborne Pathog Dis,2011,8(3):451-453.
[32]Wong-Beringer A,Wiener-Kronish J,Lynch S,et al.Comparison of type III secretion system virulence among fluoroquinolone-susceptible and-resistant clinical isolates of Pseudomonas aeruginosa[J].Clin Microbiol Infect,2008,14(4):330-336.
[33]Beceiro A,Tomás M,Bou G.Antimicrobial resistance and virulence:a successful or deleterious association in the bacterial world?[J].Clin Microbiol Rev,2013,26(2):185-230.
[2]Rudkin J K,Edwards A M,Bowden M G,et al.Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant Staphylococcusaureus by interfering with the agr quorumsensing system[J].J Infect Dis,2012,205:798-806.
[3]Hicks L A,Chien Y W,Taylor T H,et al.Outpatient antibiotic prescribing and nonsusceptible Streptococcus pneumoniae in the United States[J].Clin Infect Dis,2011,53:631-639.
[4]Huang H,Sun Y,Yuan L,et al.Regulation of the two-component regulator CpxR on aminoglycosides andβ-lactams resistance in Salmonella entericaserovar typhimurium[J].Front Microbiol,2016,7:604.
[5]Suller M T,Russell A D.Triclosan and antibiotic resistance in Staphylococcus aureus[J].J Antimicrob Chemother,2000,6(1):11-18.
[6]Catel-Ferreira M,Coadou G,Molle V,et al.Structure function relationships of CarO,the carbapenem resistance-associated outer membrane protein of Acinetobacter baumannii[J].J Antimicrob Chemother,2011,66(9):2053-2056.
[7]Sugawara E,Nikaido H.OmpA is the principal nonspecific slow porin of Acinetobacter baumannii[J].J Bacteriol,2012,194:4089-4096.
[8]Lee J S,Choi C H,Kim J W,et al.Acinetobacter baumannii outer membrane protein A induces dendritic cell death through mitochondrial targeting[J].J Microbiol,2010,48:387-392.
[9]Cabral M P,Soares N C,Aranda J,et al.Proteomic and functional analyses reveal a unique lifestyle for Acinetobacter baumannii biofilms and a key role for histidine metabolism[J].J Proteome Res,2011,10:3399-3417.
[10]Fernandez-Cuenca F,Smani Y,Gomez-Sanchez M C,et al.Attenuated virulence of a slow-growing pandrug-resistant Acinetobacter baumannii is associated with decreased expression of genes encod-ing the porins CarO and OprD-like[J].Int J Antimicrob Agents,2011,38:548-549.
[11]Bialek-Davenet S,Lavigne J P,Guyot K,et al.Differential contribution of AcrAB and OqxAB efflux pumps to multidrug resistance and virulence in Klebsiella pneumoniae[J].J Antimicrob Chemother,2015,70(1):81-88.
[12]Sun J,Deng Z,Yan A.Bacterial multidrug efflux pumps:mechanisms,physiology and pharmacological exploitations[J].Biochem Biophys Res Commun,2014,453(2):254-267.
[13]Ardebili A,Talebi M,Azimi L,et al.Effect of efflux pump inhibitor carbonyl cyanide 3-chlorophenylhydrazone on the minimum inhibitory concentration of ciprofloxacin in Acinetobacter baumannii clinical isolates[J].Jundishapur J Microbiol,2014,7(1):e8691.
[14]白国强,李昂,段美丽.鲍曼不动杆菌的耐药机制及抗生素治疗研究进展[J].山东医药,2014,54(4):90-98.
[15]Posadas D M,Martin F A,Sabio Y,et al.The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence[J].Infect Immun,2007,75:379-389.
[16]Lavigne J P,Sotto A,Nicolas-Chanoine M H,et al.Membrane permeability,apivotal func-tion involved in antibiotic resistance and virulence in Enterobacter aero-genes clinical isolates[J].Clin Microbiol Infect,2012,18:539-545.
[17]Beutlich J,Jahn S,Malorny B,et al.Antimicrobial resistance and virulence determinants in European Salmonella genomic island 1-positive Salmonella enterica isolates from different origins[J].Appl Environ Microbiol,2011,77:5655-5664.
[18]李多云,余治健,徐俊,等.利奈唑胺体外诱导粪肠球菌耐药株23SrRNA V区基因突变位点分析[J].中国病原生物学杂志,2014,4(9):316-375.
[19]Gutierrez B,Escudero J A,San Millan A,et al.Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases[J].Antimicrob Agents Chemother,2012,56:2335-2341.
[20]李科,张德纯,张名均,等.多药耐药纹带棒杆菌的重复序列PCR分型与耐药机制的研究[J].中华医院感染学杂志,2015,5(25):965-977.
[21]Habeeb M A,Haque A,Iversen A,et al.Occurrence of virulence genes,16 SrRNA methylases,and plasmid-mediated quinolone resistance genes in CTX-M-producing Escherichia coli from Pakistan[J].Eur J Clin Microbiol Infect Dis,2014,33(3):399-409.
[22]邓琼,徐群,飞刘洋,等.肺炎克雷伯菌喹诺酮类药物耐药表型与PMQR基因携带相关性研究[J].中华医院感染学杂志,2016,18(26):4081-4083.
[23]Pérez-Llarena F J,Fernández A,Zamorano L,et al.Characterization of a novel IMP-28metallo-β-lactamase from a Spanish Klebsiella oxytocaclinical isolate[J].Antimicrob Agents Chemother,2012,56(8):4540-4543.
[24]Emaneini M,Bigverdi R,Kalantar D,et al.Distribution of genes encoding tetracycline resistance and aminoglycoside modifying enzymes in Staphylococcus aureus strains isolated from a burn center[J].Ann Burns Fire Disasters,2013,26(2):76-80.
[25]刘珍,阙凤霞,李嫄渊,等.寒沙门菌质粒pRST98对细菌生物膜形成能力及毒力的影响[J].检验医学2015,30(10):1033-1038.
[26]Gupta P,Sothiselvam S,Vázquez-Laslop N,et al.Deregulation of translation due to post-transcriptional modification of rRNA explains why erm genes are inducible[J].Nat Commun,2013,4:1984.
[27]Rice L B.Mechanisms of resistance and clinical relevance of resistance toβ-lactams,glycopeptides,and fluoroquinolones[J].Mayo Clin Proc,2012,87(2):198-208.
[28]李军,陈桂林.产ESBLs大肠埃希菌和肺炎克雷伯菌的耐药性分析及分布[J].河北医药,2016,18(38):2857-2891.
[29]刘泽世,呼瑞,张毅,等.非多重耐药及多重耐药鲍曼不动杆菌外排泵及其表达的研究[DB/OL].http://www.cnki.net/kcms/detail/61.1399.R.20161010.1814.014.
[30]Smani Y,López-Rojas R,Domínguez-Herrera J,et al.In vitro and in vivo reduced fitness and virulence in ciprofloxacin-resistant Acinetobacter baumannii[J].Clin Microbiol Infect,2012,18(1):E1-4.
[31]Tran Q T,Nawaz M S,Deck J,et al.Detection of type III secretion system virulence and mutations in gyrA and parC genes among quinolone-resistant strains of Pseudomonas aeruginosa isolated from imported shrimp[J].Foodborne Pathog Dis,2011,8(3):451-453.
[32]Wong-Beringer A,Wiener-Kronish J,Lynch S,et al.Comparison of type III secretion system virulence among fluoroquinolone-susceptible and-resistant clinical isolates of Pseudomonas aeruginosa[J].Clin Microbiol Infect,2008,14(4):330-336.
[33]Beceiro A,Tomás M,Bou G.Antimicrobial resistance and virulence:a successful or deleterious association in the bacterial world?[J].Clin Microbiol Rev,2013,26(2):185-230.