金合欢醇和烟碱对苹果黄蚜联合毒杀作用
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摘要
以苹果黄蚜AphiscitricolavanderGoot为试虫,对金合欢醇和烟碱的联合杀蚜活性进行了测定,以期为开发植物源杀蚜剂产品奠定基础。室内毒力测定采用微量点滴法;田间药效试验参照国家标准进行。金合欢醇和烟碱混用具有明显的杀蚜增效作用,在最佳质量配比(金合欢醇:烟碱=4.82:1)下,共毒系数(CTC)达151.63;经过对溶剂、表面活性剂等助剂的筛选,研制出6%烟碱·金合欢醇可溶液剂,配方为金合欢醇(5%)、烟碱(1%)、表面活性剂(10%)、溶剂(84%),质量检测符合可溶液剂国家质量标准;田间药效试验表明,以126.0 g a.i./hm~2常量喷雾,药后7 d对小麦上的麦长管蚜Sitobion avenae Fabricius和麦二叉蚜Schizaphis graminum(Rondani)混合种群的防效达87.80%,以115.5 g a.i./hm~2常量喷雾,药后7d对苹果黄蚜的防效在81%以上。可见,金合欢醇和烟碱复配具有显著的杀蚜增效作用,具有进一步开发潜力。
For the development of new botanical aphicides, the joint aphidicidal action of farnesol and nicotine against Aphis citricolavander Goot was evaluated using bioassay by topical application and field trial according to the China national standard. The mixture of farnesol and nicotine showed significant synergy effect against A. citricolavander, with a co-toxicity coefficient(CTC) of 151.63 at the optimal ratio of farnesol to nicotine(4.82:1, w/w). Based on the result of solvents and surfactants screening, a 6% farnesol·nicotine soluble concentrate(6% F·N SL) was developed, which consisted of 5% farnesol, 1% nicotine, 10% surfactants, and 84% solvents, with quality measuring up with the National SL Quality Standard. Field trials showed a 87.80% control efficiency by 126.0 g a.i./hm~2 of 6% F·N SL against the mixed populations of Sitobion avenae Fabricius and Schizaphis graminum(Rondani) at 7 d post spraying, and an above 81% control efficiency by 115.5 g a.i./hm~2 of 6% F·N SL against Aphis citricolavander Goot at 7 d post spraying. Thus, the mixture of farnesol and nicotine has a significant synergy effect against aphids and is of potential to be further developed as a botanical pesticide.
For the development of new botanical aphicides, the joint aphidicidal action of farnesol and nicotine against Aphis citricolavander Goot was evaluated using bioassay by topical application and field trial according to the China national standard. The mixture of farnesol and nicotine showed significant synergy effect against A. citricolavander, with a co-toxicity coefficient(CTC) of 151.63 at the optimal ratio of farnesol to nicotine(4.82:1, w/w). Based on the result of solvents and surfactants screening, a 6% farnesol·nicotine soluble concentrate(6% F·N SL) was developed, which consisted of 5% farnesol, 1% nicotine, 10% surfactants, and 84% solvents, with quality measuring up with the National SL Quality Standard. Field trials showed a 87.80% control efficiency by 126.0 g a.i./hm~2 of 6% F·N SL against the mixed populations of Sitobion avenae Fabricius and Schizaphis graminum(Rondani) at 7 d post spraying, and an above 81% control efficiency by 115.5 g a.i./hm~2 of 6% F·N SL against Aphis citricolavander Goot at 7 d post spraying. Thus, the mixture of farnesol and nicotine has a significant synergy effect against aphids and is of potential to be further developed as a botanical pesticide.
引文
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[2]冯磊,黄晓磊,乔格侠.福建省蚜虫类昆虫多样性研究[J].动物分类学报,2004,29(4):666-674.
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[6]Will T,Vilcinskas A.Aphid-proof plants:biotechnology-based approaches for aphid control[J].Advance in Biochemical Engineering/Biotechnology,2013(136):179-203.
[7]Bala A E A,Delorme R,Kollmann A,et al.Insecticidal activity of daphnane diterpenes from Lasiosiphon Kraussianus(Meisn)(Thymelaeaceae)roots[J].Pesticide Science,1999,55(7):745-750.
[8]Copping L G,Duke S O.Natural products that have been used commercially as crop protection agents[J].Pest Management Science,2007,63(6):524-554.
[9]邹华娇.9%辣椒碱·烟碱微乳剂防治菜青虫和菜蚜效果试验[J].植物保护,2002,28(1):45-47.
[10]霍鑫,穆荣娟,何军,等.苦豆子碱和烟碱的联合杀蚜作用[J].昆虫学报,2014,57(5):557-563.
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[12]李毅平,姜在阶.外源蜕皮激素和保幼激素类似物对长角血蜱生殖的影响[J].寄生虫与医学昆虫学报,1995(4):235-242.
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[16]张宗炳.杀虫药剂的毒力测定:原理·方法·应用[M].北京:科学出版社,1988.
[17]Sun Y P,Johnson E R.Analysis of joint action of insecticides against house flies[J].Journal of Economic Entomology,1960,53(5):887-892.
[18]GB/T1601-93.农药pH值的测定方法[S].1993.
[19]GB/T19136-2003.农药热贮稳定性测定方法[S].2003.
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[21]骆焱平,宋薇薇.农药制剂加工技术[M].北京:化学工业出版社,2015.
[22]GB/T 17980.79-2004.杀虫剂防治小麦蚜虫田间药效试验准则[S].2004.
[23]GB/T 17980.9-2000.农药田间药效试验准则(一)杀虫剂防治果树蚜虫[S].2000.
[24]吴文君.植物杀虫剂苦皮藤素V作用靶标和作用机理研究进展[J].农药,2016,55(8):547-550.
[25]IRAC Mode of Action Classification Scheme Version:8.3[M].Insecticide Resistance Action Committee,2017,6.
[26]刘雨晴,范毅,于立芹,等.天然苦皮藤素和天然除虫菊素混配对三种蚜虫的毒力及田间防效[J].植物保护,2014,40(2):175-178.
[27]Millar N S,Denholm I.Nicotinic acetylcholine receptors:targets for commercially important insecticides[J].Invert Neurosci,2007,7(1):53-66.
[28]Mayoral J G,Nouzova M,Navare A.NADP+-dependent farnesol dehydrogenase,a corpora allata enzyme involved in juvenile hormone synthesis[J].Proceeding of the National Academy of Science of the United States of America,2009,106(50):21091-21096.
[29]Sen S E,Sperry A E,Childress M.Juvenile hormone biosynthesis in moths:synthesis and evaluation of farnesol homologs as alternate substrates of farnesol oxidase[J].Insect Biochemistry and Molecular Biology,2003,33(6):601-607.
[30]Tak J H,Isman M B.Enhanced cuticular penetration as the mechanism for synergy of insecticidal constituents of rosemary essential oil in Trichoplusia ni[J].Scientific Reports,2015(5):12690.
[31]Enayati A A,Ranson H,Hemingway J.Insect glutathione transferases and insecticide resistance[J].Insect Molecular Biology,2005,14(1):3-8.
[32]刘畅,王芳,张蓉,等.乳浆大戟植物杀虫水乳剂对枸杞蚜虫的毒力测定及田间防效[J].农药,2014,53(9):680-682.
[33]Ku C M,Lin J Y.Farnesol,a sesquiterpene alcohol in essential oils,ameliorates serum allergic antibody titres and lipid profiles in ovalbumin-challenged mice[J].Allergologia Et Immunopathologia,2016,44(2):149-159.
[34]Wang F J,Liu Z H,Zhang D,et al.In vitro activity of farnesol against vaginal Lactobacillus spp.[J].European Journal of Obstetrics and Gynecology,2017(212):25-29.
[35]Lapczynski A,Bhatia S P,Letizia C S,et al.Fragrance material review on farnesol[J].Food and Chemical Toxicology,2008(46):149-156.
[2]冯磊,黄晓磊,乔格侠.福建省蚜虫类昆虫多样性研究[J].动物分类学报,2004,29(4):666-674.
[3]Nault L.Arthropod transmission of plant viruses:a new synthesis[J].Annals of the Entomological Society of American,1997,90(5):521-541.
[4]唐平华,陈国平,朱明库,等.蚜虫防治技术研究进展[J].植物保护,2013,39(2):5-12.
[5]Ahmad M,Arif M I.Susceptibility of Pakistani population of cotton aphid Aphis gossypii(Homoptera:Aphididae)to endosulfan,organophosphorus and carbamate insecticides[J].Crop Protection,2008,27(3-5):523-531.
[6]Will T,Vilcinskas A.Aphid-proof plants:biotechnology-based approaches for aphid control[J].Advance in Biochemical Engineering/Biotechnology,2013(136):179-203.
[7]Bala A E A,Delorme R,Kollmann A,et al.Insecticidal activity of daphnane diterpenes from Lasiosiphon Kraussianus(Meisn)(Thymelaeaceae)roots[J].Pesticide Science,1999,55(7):745-750.
[8]Copping L G,Duke S O.Natural products that have been used commercially as crop protection agents[J].Pest Management Science,2007,63(6):524-554.
[9]邹华娇.9%辣椒碱·烟碱微乳剂防治菜青虫和菜蚜效果试验[J].植物保护,2002,28(1):45-47.
[10]霍鑫,穆荣娟,何军,等.苦豆子碱和烟碱的联合杀蚜作用[J].昆虫学报,2014,57(5):557-563.
[11]Sperry A E,Sen S E.Farnesol oxidation in insects:evidence that the biosynthesis of insect juvenile hormone is mediated by a specific alcohol oxidase[J].Insect Biochemistry and Molecular Biology,2001,31(2):171-178.
[12]李毅平,姜在阶.外源蜕皮激素和保幼激素类似物对长角血蜱生殖的影响[J].寄生虫与医学昆虫学报,1995(4):235-242.
[13]Perri D,Gorosito N,Fernandez P,et al.Plant-based compounds with potential as push-pull stimuli to manage behavior of leaf-cutting ants[J].Entomologia Experimentalis et Applicata,2017,163(2):150-159.
[14]吴文君.植物化学保护实验技术导论[M].西安:陕西科学技术出版社,1988.
[15]Mansour N,Eldefrawi M,Toppozada A,et al.Toxicological studies on the Egyptian cotton leaf worm,Prodenia litura.VI.Potentiation and antagonism of organophosphorus and carbamate insecticides[J].Journal of Economic Entomology,1966,59(3):307-311.
[16]张宗炳.杀虫药剂的毒力测定:原理·方法·应用[M].北京:科学出版社,1988.
[17]Sun Y P,Johnson E R.Analysis of joint action of insecticides against house flies[J].Journal of Economic Entomology,1960,53(5):887-892.
[18]GB/T1601-93.农药pH值的测定方法[S].1993.
[19]GB/T19136-2003.农药热贮稳定性测定方法[S].2003.
[20]GB/T19137-2003.农药冷贮稳定性测定方法[S].2003.
[21]骆焱平,宋薇薇.农药制剂加工技术[M].北京:化学工业出版社,2015.
[22]GB/T 17980.79-2004.杀虫剂防治小麦蚜虫田间药效试验准则[S].2004.
[23]GB/T 17980.9-2000.农药田间药效试验准则(一)杀虫剂防治果树蚜虫[S].2000.
[24]吴文君.植物杀虫剂苦皮藤素V作用靶标和作用机理研究进展[J].农药,2016,55(8):547-550.
[25]IRAC Mode of Action Classification Scheme Version:8.3[M].Insecticide Resistance Action Committee,2017,6.
[26]刘雨晴,范毅,于立芹,等.天然苦皮藤素和天然除虫菊素混配对三种蚜虫的毒力及田间防效[J].植物保护,2014,40(2):175-178.
[27]Millar N S,Denholm I.Nicotinic acetylcholine receptors:targets for commercially important insecticides[J].Invert Neurosci,2007,7(1):53-66.
[28]Mayoral J G,Nouzova M,Navare A.NADP+-dependent farnesol dehydrogenase,a corpora allata enzyme involved in juvenile hormone synthesis[J].Proceeding of the National Academy of Science of the United States of America,2009,106(50):21091-21096.
[29]Sen S E,Sperry A E,Childress M.Juvenile hormone biosynthesis in moths:synthesis and evaluation of farnesol homologs as alternate substrates of farnesol oxidase[J].Insect Biochemistry and Molecular Biology,2003,33(6):601-607.
[30]Tak J H,Isman M B.Enhanced cuticular penetration as the mechanism for synergy of insecticidal constituents of rosemary essential oil in Trichoplusia ni[J].Scientific Reports,2015(5):12690.
[31]Enayati A A,Ranson H,Hemingway J.Insect glutathione transferases and insecticide resistance[J].Insect Molecular Biology,2005,14(1):3-8.
[32]刘畅,王芳,张蓉,等.乳浆大戟植物杀虫水乳剂对枸杞蚜虫的毒力测定及田间防效[J].农药,2014,53(9):680-682.
[33]Ku C M,Lin J Y.Farnesol,a sesquiterpene alcohol in essential oils,ameliorates serum allergic antibody titres and lipid profiles in ovalbumin-challenged mice[J].Allergologia Et Immunopathologia,2016,44(2):149-159.
[34]Wang F J,Liu Z H,Zhang D,et al.In vitro activity of farnesol against vaginal Lactobacillus spp.[J].European Journal of Obstetrics and Gynecology,2017(212):25-29.
[35]Lapczynski A,Bhatia S P,Letizia C S,et al.Fragrance material review on farnesol[J].Food and Chemical Toxicology,2008(46):149-156.