老鹳草微胶囊复合织物的制备及其药物释放机制
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摘要
为制备可持续释放,缓释时间较长的载药复合织物,采用界面聚合法,以聚乙烯醇为壁材,中药老鹳草水提取物为芯材制备得到老鹳草微胶囊。以水性聚氨酯为黏合剂,通过干法涂层将老鹳草微胶囊整理到纯棉针织物上制备得到复合织物。采用扫描电子显微镜、热重分析仪、紫外-可见光谱等对老鹳草微胶囊及复合织物的形貌、耐热性、缓释性进行测试与表征。结果表明:老鹳草微胶囊表面光滑,平均粒径为23.9μm,载药率为33.0%,在325℃以下具有较好的热稳定性;制备的老鹳草微胶囊具有缓释性能,其释放遵循非菲克扩散规律,在缓冲溶液中可持续释放24 h以上;复合织物中药物释放遵循同样的规律,在缓冲液中的释放时间有所延长。
In order to control the release speed of the drug in the composite fabric and prolong its release process,the Geranium wilfordii Maxim(GWM) extracts were encapsulated into polyvinyl alcohol(PVA)by interfacial polymerization.The GWM microcapsules were coated on cotton knits using water-borne polyurethane(PU) adhesive by direct coating.The morphology,particle size,thermal stabilityand slowrelease properties of the microcapsules and the morphology and slow-release properties of the composite fabric were studied using SEM,TG andultraviolet-visible(UV-VIS) spectrophotometer.The results indicate that the surface of GWM/PVA microcapsules is smooth,and the average particle size is 23.9 μm.The GWM/PVA microcapsules with a drug loading ratio of 33.0% show good thermal stability at 325 ℃.The release process following the non-fickian diffusion mechanism can last for more than 24 h in the buffer solution.The release process of the composite fabric follows the similar diffusion mechanism.The release time is prolonged in the buffer solution.
In order to control the release speed of the drug in the composite fabric and prolong its release process,the Geranium wilfordii Maxim(GWM) extracts were encapsulated into polyvinyl alcohol(PVA)by interfacial polymerization.The GWM microcapsules were coated on cotton knits using water-borne polyurethane(PU) adhesive by direct coating.The morphology,particle size,thermal stabilityand slowrelease properties of the microcapsules and the morphology and slow-release properties of the composite fabric were studied using SEM,TG andultraviolet-visible(UV-VIS) spectrophotometer.The results indicate that the surface of GWM/PVA microcapsules is smooth,and the average particle size is 23.9 μm.The GWM/PVA microcapsules with a drug loading ratio of 33.0% show good thermal stability at 325 ℃.The release process following the non-fickian diffusion mechanism can last for more than 24 h in the buffer solution.The release process of the composite fabric follows the similar diffusion mechanism.The release time is prolonged in the buffer solution.
引文
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[17]窦鹏,张华,张雯.盐酸青藤碱聚乙烯醇微胶囊的制备及体外释放机制考察[J].中国实验方剂学杂志,2012,18(24):49-52.DOU Peng,ZHANG Hua,ZHANG Wen.Optimization of preparation technology and investigation of in virto release mechanism of sinomenine hydrocholoridepolyvinyl alcohol microcapsules[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(24):49-52.
[2]CHAKKARAPANI P,SUBBIAH L,PALANISAMY S,et al.Encapsulation of methotrexate loaded magnetic microcapsules for magnetic drug targeting and controlled drug release[J].Journal of Magnetism and Magnetic Materials,2015,380:285-294.
[3]SHI F,ZHANG Y,YANG G,et al.Preparation of a micro/nanotechnology based multi-unit drug delivery system for a Chinese medicine Niuhuang Xingxiao Wan and assessment of its antitumor efficacy[J].International Journal of Pharmaceutics,2015,492:244-247.
[4]许时婴,张晓鸣,夏书芹.微胶囊技术:原理与应用[M].北京:化学工业出版社,2006:170-175.XU Shiying,ZHANG Xiaoming,XIA Shuqin.Microcapsule Technology:Theory and Application[M].Beijing:Chemical Industry Press,2006:170-175.
[5]COSTA P.An alternative method to the evaluation of similarity factor in dissolution testing[J].International Journal of Pharmaceutics,2001,220:77-83.
[6]JAIN A,JAIN S K.In vitro release kinetics model fitting of liposomes:an insight[J].Chemistry&Physics of Lipids,2016,201:28-40.
[7]HUANBUTTA K,NERNPLOD T,AKKARA MONGKOLPORN P,et al.Design of porous EudragitL beads for floating drug delivery by wax removal technique[J].Asian Journal of Pharmaceutical Sciences,2016,12(3):227-234.
[8]贺海波,熊超,郭力城,等.ITS2序列鉴定多基原药材老鹳草[J].中国药学杂志,2015(17):1505-1511.HE Haibo,XIONG Chao,GUO Licheng,et al.Identification of Geranii herbae using ITS2 sequence[J].Chinese Journal of Pharmaceuticals,2015(17):1505-1511
[9]周庆颂,孙若飞,李笑然,等.不同产地老鹳草中有效成分的测定[J].华西药学杂志,2015,30(4):465-468.ZHOU Qingsong,SUN Ruofei,LI Xiaoran,et al.Determination of Components in Geranii Herba form the different origins[J].West China Journal Pharmaceutical Sciences,2015,30(4):465-468.
[10]LIU D,MA Y,WANG Y,et al.One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on crosslinked 12%agarose gel[J].Journal of Separation Science,2011,34(9):995-998.
[11]MANSUR H S,SADAHIRA C M,SOUZA A N,et al.FTIR spectroscopy characterization of poly(vinyl alcohol)hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde[J].Materials Science and Engineering:C,2008,28(4):539-548.
[12]HUANG Y,ZHANG H,WAN X,et al.Carbon nanotube enhanced double-walled phase-change microcapsules for thermal energy storage[J].Journal of Materials Chemistry,2017,5(16):7482-7493.
[13]赵雪.壳聚糖胍盐酸盐的制备及其在羊毛织物抗菌整理中的应用[J].纺织学报,2015,36(4):92-96.ZHAO Xue.Preparation of chitosan guanidine hydrochloride and application thereof in antimicrobial finishing of wool fabric[J].Journal of Textile Research,2015,36(4):92-96.
[14]黄益,王权威,孟一丁,等.苎麻织物的柠檬酸/多元醇抗皱整理[J].纺织学报,2017,38(5):104-109.HUANG Yi,WANG Quanwei,MENG Yiding,et al.Anit-crease finishing of ramie fabrics with citric acid polyol[J].Journal of Textile Research,2017,38(5):104-109.
[15]陈默,王志伟,胡长鹰,等.湿度对聚乙烯醇膜阻隔性能的影响[J].高分子材料科学与工程,2009(8):61-63.CHEN Mo,WANG Zhiwei,HU Changying,et al.Effect of relative humidity on barrier properties of polyvinyl alcohol films[J].Polymer Materials Science and Engineering,2009(8):61-63.
[16]张维,李秋瑾,张健飞.层层组装微胶囊的制备及其缓释性能[J].纺织学报,2015,36(3):58-62.ZHANG Wei,LI Qiujin,ZHANG Jianfei,Preparation and controlled release of microcapsules via layer-by-layer assembly[J].Journal of Textile Research,2015,36(3):58-62.
[17]窦鹏,张华,张雯.盐酸青藤碱聚乙烯醇微胶囊的制备及体外释放机制考察[J].中国实验方剂学杂志,2012,18(24):49-52.DOU Peng,ZHANG Hua,ZHANG Wen.Optimization of preparation technology and investigation of in virto release mechanism of sinomenine hydrocholoridepolyvinyl alcohol microcapsules[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(24):49-52.