龙匙片活血化瘀作用研究
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摘要
目的通过不同试验方法和动物模型对龙匙片活血化瘀的作用进行验证,为临床合理用药提供依据。方法采用在冰水中游泳后皮下注射肾上腺素(AD)的大鼠制造急性血瘀模型,观察龙匙片对血液流变学的影响;采用AD制造肠系膜急性微循环障碍大鼠模型,观察龙匙片对微循环障碍的改善作用;采用体外法,观察龙匙片对凝血酶原时间(PT)及活化部分凝血活酶时间(APTT)的影响。结果龙匙片可显著降低大鼠的血浆黏度、全血黏度、血纤维蛋白原含量、血沉及血小板黏附率,可抑制大鼠肠系膜微循环细动脉管径缩小、流速减慢、毛细血管开放量减少及流态改变,可延长PT和APTT。结论龙匙片可改善血液流变学和微循环障碍,具有活血化瘀作用。
Objective To verify the effect of Longchi Tablets on activating blood circulation to dissipate blood stasis by several methods and animal models,and provide the evidences for its clinical use. Methods The acute blood-stasis rats models were established by hypodermic injection of adrenergic( AD) after swimming in ice water,the effect of Longchi Tablets on hemorheology was observed. The mesenteric microcirculatory disturbance rats models were established by injected with adrenergic,the effect of Longchi Tablets on microcirculatory disturbance was observed. The effect of Longchi Tablets on PT and APTT was observed by in vitro method. Results Longchi Tablets could significantly reduce the plasma viscosity,whole blood viscosity,blood fibrinogen concentration,erythrocyte sedimentation and platelet adhesion rate of the rats,it could inhibit the narrowing of the arteriole diameter of the mesenteric microcirculation,slow blood flowing rate,reduction of open capillary and the change of the blood flow pattern in rats,and could prolong the PT and APTT. Conclusion Longchi Tablets can improve the hemorheology and microcirculatory disturbance,and have the ability of activating blood circulation to dissipate blood stasis.
Objective To verify the effect of Longchi Tablets on activating blood circulation to dissipate blood stasis by several methods and animal models,and provide the evidences for its clinical use. Methods The acute blood-stasis rats models were established by hypodermic injection of adrenergic( AD) after swimming in ice water,the effect of Longchi Tablets on hemorheology was observed. The mesenteric microcirculatory disturbance rats models were established by injected with adrenergic,the effect of Longchi Tablets on microcirculatory disturbance was observed. The effect of Longchi Tablets on PT and APTT was observed by in vitro method. Results Longchi Tablets could significantly reduce the plasma viscosity,whole blood viscosity,blood fibrinogen concentration,erythrocyte sedimentation and platelet adhesion rate of the rats,it could inhibit the narrowing of the arteriole diameter of the mesenteric microcirculation,slow blood flowing rate,reduction of open capillary and the change of the blood flow pattern in rats,and could prolong the PT and APTT. Conclusion Longchi Tablets can improve the hemorheology and microcirculatory disturbance,and have the ability of activating blood circulation to dissipate blood stasis.
引文
[1]杨威,郭秋平,郭健敏,等.701跌打镇痛膏活血化瘀实验研究[J].中国药理学通报,2014,30(6):885-886.
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[18]刘翠霞,吴基良,江南,等.甘草酸二铵对动物静脉血栓形成及凝血时间的影响[J].医药导报,2004,23(11):809-810.
[19]Sharma V,Agrawal R.In vivo antioxidant and hepatoprotective potential of Glycyrrhiza glabra extract on carbon tetra chloride(CCl4)induced oxidative-stress mediated hepatotoxicity[J].Int J Res Med Sci,2014,2(1):314-320.
[20]Jiang LL,Wang Q,Shen S,et al.Discovery of glycyrrhetinic acid as an orally active,direct inhibitor of blood coagulation factor xa[J].Thromb Res,2014,133(3):501-506.
[21]李宏力,李玉文,刘天龙,等.Z-没药甾酮对急性血瘀模型大鼠凝血和血管内皮功能的改善作用及其机制研究[J].中国药房,2016,27(19):2615-2617.
[2]王琳琳,丁安伟.赤芍总苷对大鼠血瘀证模型的影响[J].南京中医药大学学报,2011,27(6):552-555.
[3]丘芬,金钊,闫守霞,等.虎杖五种酒制饮片水提物的活血化瘀作用研究[J].时珍国医国药,2013,24(12):2903-2904.
[4]李伟霞,黄美艳,唐于平,等.大鼠急性血瘀模型造模方法的研究与评价[J].中国药理学通报,2011,27(12):1761-1765.
[5]刘培,宿树兰,周卫,等.香附四物汤与四物汤对急性血瘀模型大鼠血液流变性及卵巢功能的影响[J].中国实验方剂学杂志,2010,16(8):124-127.
[6]陈奇.中药药理研究方法学(第二版)[M].北京:人民卫生出版社,2005:199-205..
[7]孙兰,李家春,周军,等.桂枝茯苓胶囊对肾上腺素诱导大鼠实验性肠系膜微循环障碍的改善研究[J].现代药物与临床,2015,30(1):8-12.
[8]邓奕辉,邓婧溪,陈懿,等.滋阴活血解毒超微中药方对脑缺血大鼠凝血酶原和蛋白酶激活受体-1 mRNA表达的影响[J].中华中医药杂志,2011,26(12):2816-2820.
[9]钟宇富,刘国洪,杜英娟,等.月之汤饮对实验大鼠血液流变性及凝血功能的影响[J].安徽医药,2016,20(2):236-239.
[10]Liu G,Zhong Y,Du Y,et al.Study on the lactation-promoting Function Experiment of Yue-nai-tang Decoction in Pregnant Female Rats[J].Medicinal Plant,2014,5(3):37-40.
[11]徐叔云,卞如濂,陈修,等.药理实验方法学[M].第3版.北京:人民卫生出版社,2002:1119-1122.
[12]林桂平,卢春颜,邝巧芬,等.复方丹参注射液抗心肌缺血及活血化瘀作用的实验研究[J].中国中医药科技,2014,21(3):251-253.
[13]Shigematsu N.Effect of administration with the extract of GS leaves on lipid metabolism in rats[J].Biological and Pharmaceutical Bulletin,2001,24(6):713-716.
[14]向金莲,程睿,张路晗.血竭的活血和止血作用研究[J].华西药学杂志,2000,15(6):430-434.
[15]胡迎庆,宫飘,屠鹏飞.龙血树属植物化学成分及生物活性研究进展[J].国外医学(植物药分册),2000,15(1):5-8.
[16]周志宏,王锦亮,杨崇仁.剑叶血竭素国产血竭中一个新的二聚查耳酮[J].药学学报,2001,36(3):200-204.
[17]李丹,惠瑞,胡永武,等.血竭提取物促进成纤维细胞增殖及胶原分泌[J].中国组织工程研究,2014,18(46):7437-7441.
[18]刘翠霞,吴基良,江南,等.甘草酸二铵对动物静脉血栓形成及凝血时间的影响[J].医药导报,2004,23(11):809-810.
[19]Sharma V,Agrawal R.In vivo antioxidant and hepatoprotective potential of Glycyrrhiza glabra extract on carbon tetra chloride(CCl4)induced oxidative-stress mediated hepatotoxicity[J].Int J Res Med Sci,2014,2(1):314-320.
[20]Jiang LL,Wang Q,Shen S,et al.Discovery of glycyrrhetinic acid as an orally active,direct inhibitor of blood coagulation factor xa[J].Thromb Res,2014,133(3):501-506.
[21]李宏力,李玉文,刘天龙,等.Z-没药甾酮对急性血瘀模型大鼠凝血和血管内皮功能的改善作用及其机制研究[J].中国药房,2016,27(19):2615-2617.