海南普通野生稻对水稻白叶枯病的抗性鉴定
|
摘要
白叶枯病严重危害水稻的生产,防治白叶枯病的工作刻不容缓。目前最有效的防治措施是培育具有抗性的水稻新品种。但是来源于栽培稻的抗病基因抗谱窄、数量有限,因此从野生稻中发掘抗白叶枯病基因,对培育具有广谱抗性的水稻新品种具有重要意义。本研究通过接种4个菲律宾白叶枯病菌小种对海南普通野生稻进行抗性鉴定,同时利用目前克隆的已知抗性基因设计分子标记进行PCR分析,检测了其中的抗白叶枯病基因。结果表明,来自海南不同地区的15个普通野生稻居群均表现出对白叶枯病菲律宾小种一定的抗性。功能标记检测结果发现,15个居群的普通野生稻中含有Xa1或其同源基因,但均不含有xa5、xa13抗病基因;7个居群能够扩增出Xa21抗病基因条带;Xa27抗病基因条带能够在另外7个居群中被扩增出。研究结果为发掘具有抗白叶枯病特性的海南普通野生稻材料提供了新的理论参考依据。
Bacterial blight(BB) seriously harms the production of rice, and the work of preventing and controlling bacterial blight is urgent. At present, the most effective prevention and cure measure is to breed new rice varieties with resistance. However, the resistance genes of rice derived from cultivated rice are narrow and limited in quantity, so it is important to explore the gene of resistance to bacterial blight from wild rice for breeding new varieties with broad spectrum resistance. In this study, the resistance of common wild rice in Hainan was evaluated by inoculating with 4 Philippine races of BB. At the same time, we designed the molecular markers with the known resistance genes, and analyzed the anti-bacterial blight genes by PCR. The results showed that 15 wild rice varieties from different parts of Hainan had certain resistance to Philippine races of BB. According to functional marker detection, 15 wild rice varieties contained Xa1 or its homologous gene, but all did not contain resistance genes xa5 and xa13. 7 populations were able to amplify Xa21 disease resistant gene bands. Xa27 resistant gene bands could be amplified in 7 other populations. The results of this study could provide a new theoretical basis for the discovery of common wild rice plants with resistance to bacterial blight in Hainan.
Bacterial blight(BB) seriously harms the production of rice, and the work of preventing and controlling bacterial blight is urgent. At present, the most effective prevention and cure measure is to breed new rice varieties with resistance. However, the resistance genes of rice derived from cultivated rice are narrow and limited in quantity, so it is important to explore the gene of resistance to bacterial blight from wild rice for breeding new varieties with broad spectrum resistance. In this study, the resistance of common wild rice in Hainan was evaluated by inoculating with 4 Philippine races of BB. At the same time, we designed the molecular markers with the known resistance genes, and analyzed the anti-bacterial blight genes by PCR. The results showed that 15 wild rice varieties from different parts of Hainan had certain resistance to Philippine races of BB. According to functional marker detection, 15 wild rice varieties contained Xa1 or its homologous gene, but all did not contain resistance genes xa5 and xa13. 7 populations were able to amplify Xa21 disease resistant gene bands. Xa27 resistant gene bands could be amplified in 7 other populations. The results of this study could provide a new theoretical basis for the discovery of common wild rice plants with resistance to bacterial blight in Hainan.
引文
Amante-Bordeos A.,Sitch L.A.,Nelson R.,Dalmacio R.D.,Oliva N.P.,Aswidinnoor H.,and Leung H.,1992,Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice,Oryza sativa,Theor Appl.Genet.,84(3-4):345-354
E Z.G.,and Wang L.,2008,Discovery and utilization of favorable genes in wild rice,Yichuan(Heredita),30(11):1397-1405(鄂志国,王磊,2008,野生稻有利基因的发掘和利用,遗传,30(11):1397-1405)
Hutin M.,Sabot F.,Ghesquière A.,Koebnik R.,and Szurek B.2015,A knowledge-based molecular screen uncovers a broad spectrum ossweet14,resistance allele to bacteria blight from wild rice,Plant J.,84(4):694-703
Li D.Q.,Chen L.,Li W.J.,Ke X.,Yu T.Q.,Li E X.,Huang X.Q.and Chen Z.Q.,2015,Identification of bacterial blight resistance gene in Yunnan wild rice,Zuowu Xuebao(Acta Agronomica Sinica),41(3):386-393(李定琴,陈玲,李维蛟,柯学,余腾琼,李娥贤,黄兴奇,程在全,2015,云南3种野生稻中抗白叶枯病基因的鉴定,作物学报,41(3):386-393)
Li W.J.,Chen L.,Yin F.Y.,Zhang D.Y.,and Chen Z.Q.,2015,Identification of resistanceg gene Xa21 in Yunnan wild rice,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(1):113-118(李维蛟,陈玲,殷富有,张敦宇,程在全,2015,云南野生稻抗白叶枯病类Xa21基因的鉴定,植物遗传资源学报,16(1):113-118)
Mo W.L.,Zhang Z.Y.,Zhao S.,Liu J.L.,Duan C.J.,and Feng J.X.,2011,Construction of X002193 gene mutant in Xanthomonas Oryzae pv.Oryzae and analysis of its virulence and extracellular polysaccharide production,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),30(5):564-570(莫伟兰,张子宇,赵帅,刘君梁,段承杰,冯家勋,2011,水稻白叶枯病菌基因xoo2193的突变体构建及其毒力和胞外多糖分析,基因组学与应用生物学,30(5):564-570)
Qin B.X.,Liu C.,Jiao X.Z.,Huang D.H.,Zhang Y.X.,Ma Z.F.,Feng J.X.,Cen Z.L.,Liu F.,Qiu Y.F.,and Li R.B.,2014,I-dentification and evaluation of Guangxi wild rice(Oryza rufipogon Griff)resources with wide spectrum resistance to bacterial blight,Nanfang Nongye Xuebao(Journal of Southern Agriculture),45(9):1527-1531(覃宝祥,刘驰,焦晓真,黄大辉,张月雄,马增凤,冯家勋,岑贞陆,刘芳,邱永福,李容柏,2014,广西普通野生稻白叶枯病广谱抗源的鉴定与评价,南方农业学报,45(9):1527-1531)
Song W.Y.,Wang G.L.,Chen L.L.,Kim H.S.,Pi L.Y.,Holsten T.,Gardner J.,Wang B.,Zhai W.X.,Zhu L.H.,Fauquet C.,and Ronald P.,1995,A receptor kinase-like protein encoded by the rice disease resistance gene,Xa21,Science,270(5243):1804-1806
Tang Q.J.,Wang X.N.,Xiong H.Y.,Chen J.X.,Rui K.,Yun Y.,2013,Investigation and identification of resistance to rice blast in Oryza rufipogon Griff.indigenous to Hainan Province,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),14(5):821-825(唐清杰,王效宁,熊怀阳,陈健晓,芮凯,云勇,2013,海南普通野生稻稻瘟病抗性资源调查和鉴定,植物遗传资源学报,14(5):821-825)
Wang C.L.,Zhang X.P.,Fan Y.L.,Gao Y.,Zhu Q.L.,Zheng C.K.Qin T.F.,Li Y.Q.,Che J.Y.,Zhang M.W.,Yang B.,Liu Y.G.and Zhao K.J.,2015,Xa23 is an executor rprotein and confers broad-spectrum disease resistance in rice,Molecular Plant,8(2):290-302
Xia Z.H.,Han F.,Gao L.F.,Yuan Q.H.,Zhai W.X.,Liu D.,and Luo Y.H.,2009,Application of functional marker to identify genes for bacterial blight resistance in Oryza rufipogon,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),23(6):653-656(夏志辉,韩飞,高利芬,袁潜华,翟文学,刘迪罗越华,2009,利用功能标记鉴定普通野生稻中的白叶枯病抗性基因,中国水稻科学,23(6):653-656)
Yun Y.,Tang Q.J.,Yan X.W.,Meng W.D.,Wang X.N.,and Lin YZ.,2015,Field survey and conservation of wild rice resources in Hainan Province,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(4):715-719(云勇,唐清杰,严小微,孟卫东,王效宁,林尤珍,2015,海南野生稻资源调查收集与保护,植物遗传资源学报,16(4):715-719)
Zhang H.H.,Liu R.,Guo H.B.,and Li Y.J.,2009,Advancement on mining and utilization of elite genes in Oryza officinalis wall,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),25(19):42-45(张欢欢,刘蕊,郭海滨,李亚娟,2009,药用野生稻有利基因发掘与利用研究进展,中国农学通报,25(19):42-45)
Zhang Q.,Wang C.L.,Zhao K.J.,Yang W.C.,Qiao F.,Zhou Y.L.,Jiang Q.X.,and Liu G.C.,2002,Development of near is ogenic line CBB23 with a new resistance gene to bacterial blight in rice and its application,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),16(3):206-210(章琦,王春连,赵开军,杨文才,乔枫,周永力,江祺祥,刘古春,2002,携有抗白叶枯病新基因Xa23水稻近等基因系的构建及应用,中国水稻科学,16(3):206-210)
E Z.G.,and Wang L.,2008,Discovery and utilization of favorable genes in wild rice,Yichuan(Heredita),30(11):1397-1405(鄂志国,王磊,2008,野生稻有利基因的发掘和利用,遗传,30(11):1397-1405)
Hutin M.,Sabot F.,Ghesquière A.,Koebnik R.,and Szurek B.2015,A knowledge-based molecular screen uncovers a broad spectrum ossweet14,resistance allele to bacteria blight from wild rice,Plant J.,84(4):694-703
Li D.Q.,Chen L.,Li W.J.,Ke X.,Yu T.Q.,Li E X.,Huang X.Q.and Chen Z.Q.,2015,Identification of bacterial blight resistance gene in Yunnan wild rice,Zuowu Xuebao(Acta Agronomica Sinica),41(3):386-393(李定琴,陈玲,李维蛟,柯学,余腾琼,李娥贤,黄兴奇,程在全,2015,云南3种野生稻中抗白叶枯病基因的鉴定,作物学报,41(3):386-393)
Li W.J.,Chen L.,Yin F.Y.,Zhang D.Y.,and Chen Z.Q.,2015,Identification of resistanceg gene Xa21 in Yunnan wild rice,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(1):113-118(李维蛟,陈玲,殷富有,张敦宇,程在全,2015,云南野生稻抗白叶枯病类Xa21基因的鉴定,植物遗传资源学报,16(1):113-118)
Mo W.L.,Zhang Z.Y.,Zhao S.,Liu J.L.,Duan C.J.,and Feng J.X.,2011,Construction of X002193 gene mutant in Xanthomonas Oryzae pv.Oryzae and analysis of its virulence and extracellular polysaccharide production,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),30(5):564-570(莫伟兰,张子宇,赵帅,刘君梁,段承杰,冯家勋,2011,水稻白叶枯病菌基因xoo2193的突变体构建及其毒力和胞外多糖分析,基因组学与应用生物学,30(5):564-570)
Qin B.X.,Liu C.,Jiao X.Z.,Huang D.H.,Zhang Y.X.,Ma Z.F.,Feng J.X.,Cen Z.L.,Liu F.,Qiu Y.F.,and Li R.B.,2014,I-dentification and evaluation of Guangxi wild rice(Oryza rufipogon Griff)resources with wide spectrum resistance to bacterial blight,Nanfang Nongye Xuebao(Journal of Southern Agriculture),45(9):1527-1531(覃宝祥,刘驰,焦晓真,黄大辉,张月雄,马增凤,冯家勋,岑贞陆,刘芳,邱永福,李容柏,2014,广西普通野生稻白叶枯病广谱抗源的鉴定与评价,南方农业学报,45(9):1527-1531)
Song W.Y.,Wang G.L.,Chen L.L.,Kim H.S.,Pi L.Y.,Holsten T.,Gardner J.,Wang B.,Zhai W.X.,Zhu L.H.,Fauquet C.,and Ronald P.,1995,A receptor kinase-like protein encoded by the rice disease resistance gene,Xa21,Science,270(5243):1804-1806
Tang Q.J.,Wang X.N.,Xiong H.Y.,Chen J.X.,Rui K.,Yun Y.,2013,Investigation and identification of resistance to rice blast in Oryza rufipogon Griff.indigenous to Hainan Province,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),14(5):821-825(唐清杰,王效宁,熊怀阳,陈健晓,芮凯,云勇,2013,海南普通野生稻稻瘟病抗性资源调查和鉴定,植物遗传资源学报,14(5):821-825)
Wang C.L.,Zhang X.P.,Fan Y.L.,Gao Y.,Zhu Q.L.,Zheng C.K.Qin T.F.,Li Y.Q.,Che J.Y.,Zhang M.W.,Yang B.,Liu Y.G.and Zhao K.J.,2015,Xa23 is an executor rprotein and confers broad-spectrum disease resistance in rice,Molecular Plant,8(2):290-302
Xia Z.H.,Han F.,Gao L.F.,Yuan Q.H.,Zhai W.X.,Liu D.,and Luo Y.H.,2009,Application of functional marker to identify genes for bacterial blight resistance in Oryza rufipogon,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),23(6):653-656(夏志辉,韩飞,高利芬,袁潜华,翟文学,刘迪罗越华,2009,利用功能标记鉴定普通野生稻中的白叶枯病抗性基因,中国水稻科学,23(6):653-656)
Yun Y.,Tang Q.J.,Yan X.W.,Meng W.D.,Wang X.N.,and Lin YZ.,2015,Field survey and conservation of wild rice resources in Hainan Province,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(4):715-719(云勇,唐清杰,严小微,孟卫东,王效宁,林尤珍,2015,海南野生稻资源调查收集与保护,植物遗传资源学报,16(4):715-719)
Zhang H.H.,Liu R.,Guo H.B.,and Li Y.J.,2009,Advancement on mining and utilization of elite genes in Oryza officinalis wall,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),25(19):42-45(张欢欢,刘蕊,郭海滨,李亚娟,2009,药用野生稻有利基因发掘与利用研究进展,中国农学通报,25(19):42-45)
Zhang Q.,Wang C.L.,Zhao K.J.,Yang W.C.,Qiao F.,Zhou Y.L.,Jiang Q.X.,and Liu G.C.,2002,Development of near is ogenic line CBB23 with a new resistance gene to bacterial blight in rice and its application,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),16(3):206-210(章琦,王春连,赵开军,杨文才,乔枫,周永力,江祺祥,刘古春,2002,携有抗白叶枯病新基因Xa23水稻近等基因系的构建及应用,中国水稻科学,16(3):206-210)