云南30个水稻品种对水稻白叶枯病的抗性评价
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摘要
【目的】水稻白叶枯病是一种严重危害水稻的细菌性病害,培育抗性品种是治理该病害的重要途径。因此,明确云南水稻材料对检疫性病害水稻白叶枯病的抗性,可以为该病害的防治与监测、水稻栽培的合理布局和良好抗性资源的获取提供依据。【方法】采用剪叶接种法测定云南稻区30个品种对7个不同致病型白叶枯病菌的抗性。【结果】在供试的30个云南水稻品种中,2个品种(玉粳16和JS42糯稻)对7个不同致病型菌株均表现为抗性;15个品种对7个致病型菌株均表现感病;对HEN11、SCYC-6、YN7、YN11、FUJ、YN241和PX099等7个致病型菌株表现抗性的水稻品种分别占26.67%、16.67%、23.33%、13.33%、6.67%、10.00%和20.00%。此外,区试材料的抗性比例高于主栽品种,地方稻未发现抗性品种。【结论】现在生产上的大部分水稻品种对优势致病型病原菌入侵的抵抗能力降低甚至丧失。针对云南地区的优势致病小种FUJ筛选得到2个抗性品种:玉粳16和JS42糯稻。
【Aim】Rice bacterial blight is a serious disease of this major staple crop. Landraces often contain resistence genes useful for breeding,so testing such cultivarscan provide a basis for the prevention and control of rice diseases. 【Method】Resistance to 7different pathogenic Xanthomonas oryzae pv. oryzae strains of 30 local rice cultivars from Yunnan Province was tested by leaf inoculation. 【Result】Among the 30 tested rice cultivars,two showed resistance to all 7 pathogenic strains,while 15 cultivars were susceptible to all pathogenic strains tested. The measured levels of resistance to the 7 different pathogenic strains ranged from 6. 67% to26.67%. In addition,the resistance ratio of regional trial materials was higher than that of the main cultivars. No resistance was found in the local rice.【Conclusion】Most of the cultivars in production have reduced or lost ability to withstand the dominant pathogenic bacteria. Cv.s Yujing 16 and Nuodao JS42 were the resistant cultivars against the dominant pathogenic race FUJ in Yunnan Province.
【Aim】Rice bacterial blight is a serious disease of this major staple crop. Landraces often contain resistence genes useful for breeding,so testing such cultivarscan provide a basis for the prevention and control of rice diseases. 【Method】Resistance to 7different pathogenic Xanthomonas oryzae pv. oryzae strains of 30 local rice cultivars from Yunnan Province was tested by leaf inoculation. 【Result】Among the 30 tested rice cultivars,two showed resistance to all 7 pathogenic strains,while 15 cultivars were susceptible to all pathogenic strains tested. The measured levels of resistance to the 7 different pathogenic strains ranged from 6. 67% to26.67%. In addition,the resistance ratio of regional trial materials was higher than that of the main cultivars. No resistance was found in the local rice.【Conclusion】Most of the cultivars in production have reduced or lost ability to withstand the dominant pathogenic bacteria. Cv.s Yujing 16 and Nuodao JS42 were the resistant cultivars against the dominant pathogenic race FUJ in Yunnan Province.
引文
李定琴,钟巧芳,曾民,陈越,王波,程在全,2017.水稻抗白叶枯病基因定位、克隆及利用研究进展.中国稻米,23(5):19-27.
李栋,何美丹,吴丹,赵建发,何光亮,夏志辉,袁潜华,2018.海南普通野生稻对水稻白叶枯病的抗性鉴定.分子植物育种,16(3):832-839.
史波,吴云雨,陈浩,管昌红,刘凤权,张红生,鲍永美,2016.6省水稻主栽品种对白叶枯病菌的抗性鉴定.南京农业大学学报,39(3):349-357.
王明明,张帆,石英尧,卓大龙,胡丹丹,周永力,黎志康,2017.我国水稻微核心种质资源对白叶枯病抗性的鉴定和评价.植物遗传资源学报,18(4):771-777.
吴宪,许晶,温嘉伟,刘文平,张佳环,王继春,2015.东北水稻白叶枯病菌株遗传多样性分析及品种对白叶枯病抗性评价.吉林农业大学学报,37(3):290-295.
肖素勤,殷富有,程在全,2017.云南野生稻资源及其保护.植物遗传资源学报,18(3):494-502.
徐海根,强胜,2004.中国外来入侵物种编目.北京:中国环境科学出版社.
徐颖,祝晓芬,周明国,2013.水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价.农药学学报,15(1):43-51.
杨雅云,张恩来,阿新祥,汤翠凤,张斐斐,陈丹,董超,吴坤,徐福荣,周英,祁春学,戴陆园,2014.云南高原粳稻白叶枯病菌的抗药性室内鉴定及其rpf C基因序列分析.中国水稻科学,28(6):665-674.
余腾琼,肖素勤,程在全,2016.云南野生稻和地方稻资源抗白叶枯病分析.植物保护学报,43(5):774-781.
俞咪娜,于俊杰,尹小乐,2014.2013年江苏省水稻区试品种抗细菌性病害的鉴定和评价.江苏农业科学,42(8):109-110.
袁筱萍,魏兴华,徐群,王一平,余汉勇,王彩红,2016.中国主栽品种对7个白叶枯病菌菌株的抗性分析.植物保护,42(4):169-173.
朱烨琳,梁晓宇,徐曙,候毅平,王建新,周明国,2014.农药学学报,16(2):125-131.
CHEN H,WANG S P,ZHANG Q F,2002.New gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63,an elite restorer line.Phytopathology,92(7):750-754.
IYER A S,MCCOUCH S R,2004.The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance.Molecular Plant-microbe Interactions,17(12):1348-1354.
KAUFFMAN H E,REDDY A P K,HSIEH S P Y,1973.Improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae.Plant Disease Reporter,57:537-541.
KUMAR A,GUHA A,BIMOLATA W,REDDY A R,LAHA GS,SUNDARAM R M,GHAZI I A,2013.Leaf gas exchange physiology in rice genotypes infected with bacterial blight:an attempt to link photosynthesis with disease severity and rice yield.Australian Journal of Crop Science,7(1):32.
LIU Q,YUAN M,ZHOU Y,LI X,WANG S,2011.A paralog of the Mt N3/saliva family recessively confers race-specific re-sistance to Xanthomonas oryzae in rice.Plant Cell and Envi-ronment,34(11):1958-1969.
LOS B,IRRI,1996.Standard evaluation system for rice.the Philippines:International Rice Research Institute.
SONG W Y,WANG G L,CHEN L L,HOLSTEN T,1995.Areceptor kinase-like protein encoded by the rice disease resistance gene,Xa21.Science,270:1804-1806.
SUN X,CAO Y,YANG Z,XU C,LI X,WANG S,2004.Xa26,a gene conferring resistance to Xanthomonas oryzae pv.oryzae in rice,encodes an LRR receptor kinase-like protein.Plant Journal,37(4):517-527.
VIKAL Y,BHATIA D,2017.Genetics and genomics of bacterial blight resistance in rice.London,UK:In Tech.
WANG C,ZHANG X,FAN Y,GAO Y,ZHU Q,ZHENG C,2015.XA23 is an executor R protein and confers broadspectrum disease resistance in rice.Molecular Plant,8(2):290-302.
YOSHIMURA A,MEW T W,KHUSH G S,1983.Inheritance of resistance to bacterial blight in rice cultivar Cas 209.Genetics,73(10):1409-1412.
李栋,何美丹,吴丹,赵建发,何光亮,夏志辉,袁潜华,2018.海南普通野生稻对水稻白叶枯病的抗性鉴定.分子植物育种,16(3):832-839.
史波,吴云雨,陈浩,管昌红,刘凤权,张红生,鲍永美,2016.6省水稻主栽品种对白叶枯病菌的抗性鉴定.南京农业大学学报,39(3):349-357.
王明明,张帆,石英尧,卓大龙,胡丹丹,周永力,黎志康,2017.我国水稻微核心种质资源对白叶枯病抗性的鉴定和评价.植物遗传资源学报,18(4):771-777.
吴宪,许晶,温嘉伟,刘文平,张佳环,王继春,2015.东北水稻白叶枯病菌株遗传多样性分析及品种对白叶枯病抗性评价.吉林农业大学学报,37(3):290-295.
肖素勤,殷富有,程在全,2017.云南野生稻资源及其保护.植物遗传资源学报,18(3):494-502.
徐海根,强胜,2004.中国外来入侵物种编目.北京:中国环境科学出版社.
徐颖,祝晓芬,周明国,2013.水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价.农药学学报,15(1):43-51.
杨雅云,张恩来,阿新祥,汤翠凤,张斐斐,陈丹,董超,吴坤,徐福荣,周英,祁春学,戴陆园,2014.云南高原粳稻白叶枯病菌的抗药性室内鉴定及其rpf C基因序列分析.中国水稻科学,28(6):665-674.
余腾琼,肖素勤,程在全,2016.云南野生稻和地方稻资源抗白叶枯病分析.植物保护学报,43(5):774-781.
俞咪娜,于俊杰,尹小乐,2014.2013年江苏省水稻区试品种抗细菌性病害的鉴定和评价.江苏农业科学,42(8):109-110.
袁筱萍,魏兴华,徐群,王一平,余汉勇,王彩红,2016.中国主栽品种对7个白叶枯病菌菌株的抗性分析.植物保护,42(4):169-173.
朱烨琳,梁晓宇,徐曙,候毅平,王建新,周明国,2014.农药学学报,16(2):125-131.
CHEN H,WANG S P,ZHANG Q F,2002.New gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63,an elite restorer line.Phytopathology,92(7):750-754.
IYER A S,MCCOUCH S R,2004.The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance.Molecular Plant-microbe Interactions,17(12):1348-1354.
KAUFFMAN H E,REDDY A P K,HSIEH S P Y,1973.Improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae.Plant Disease Reporter,57:537-541.
KUMAR A,GUHA A,BIMOLATA W,REDDY A R,LAHA GS,SUNDARAM R M,GHAZI I A,2013.Leaf gas exchange physiology in rice genotypes infected with bacterial blight:an attempt to link photosynthesis with disease severity and rice yield.Australian Journal of Crop Science,7(1):32.
LIU Q,YUAN M,ZHOU Y,LI X,WANG S,2011.A paralog of the Mt N3/saliva family recessively confers race-specific re-sistance to Xanthomonas oryzae in rice.Plant Cell and Envi-ronment,34(11):1958-1969.
LOS B,IRRI,1996.Standard evaluation system for rice.the Philippines:International Rice Research Institute.
SONG W Y,WANG G L,CHEN L L,HOLSTEN T,1995.Areceptor kinase-like protein encoded by the rice disease resistance gene,Xa21.Science,270:1804-1806.
SUN X,CAO Y,YANG Z,XU C,LI X,WANG S,2004.Xa26,a gene conferring resistance to Xanthomonas oryzae pv.oryzae in rice,encodes an LRR receptor kinase-like protein.Plant Journal,37(4):517-527.
VIKAL Y,BHATIA D,2017.Genetics and genomics of bacterial blight resistance in rice.London,UK:In Tech.
WANG C,ZHANG X,FAN Y,GAO Y,ZHU Q,ZHENG C,2015.XA23 is an executor R protein and confers broadspectrum disease resistance in rice.Molecular Plant,8(2):290-302.
YOSHIMURA A,MEW T W,KHUSH G S,1983.Inheritance of resistance to bacterial blight in rice cultivar Cas 209.Genetics,73(10):1409-1412.