木本植物游离小孢子培养研究进展
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摘要
木本植物生长周期长,传统方法育种低效,且木本植物带菌系数高、体内含有较多易抑制培养物生长的酚类化合物等,使得木本植物再生体系建立困难,从而制约了利用生物技术进行遗传改良和相关功能基因的研究进展。植物游离小孢子培养是植物获得单倍体重要途径之一,通过诱导培养能再生成纯合的二倍体植株,不仅加快木本植物遗传改良,也为木本植物的苗木繁育、分子生物学、遗传学和形态发生学等研究提供稳定的材料。本综述主要介绍当前木本植物小孢子培养的一些关键步骤及其影响成败的主要因素、研究现状和存在问题,并展望了该技术在木本植物中的应用前景。
It is inefficient to use traditional breeding methods for woody plants because of their long growth cycles. Meanwhile, due to a high coefficient of woody plants contamination and containing more phenolic compounds in the body, which were easy to suppress the culture growth, so it is difficult to establish regeneration systems for most of woody plants. Thus, those factors restricted the research progress in genetic improvement and related functional genes of woody plants by using the biotechnology. Isolated microspore culture, as an important way to obtain haploid, which could generate homozygous diploid plant after induction culture, not only speeds up the genetic improvement of woody plants, but also provides stable research materials for seedling breeding,molecular biology, genetics and morphological genology of woody plants. In this review, we mainly introduced the basic steps and the key factors influencing the success in microspore culture of woody plants, the research status and existing problems, and prospected the application foreground of the technology in woody plants in the future.
It is inefficient to use traditional breeding methods for woody plants because of their long growth cycles. Meanwhile, due to a high coefficient of woody plants contamination and containing more phenolic compounds in the body, which were easy to suppress the culture growth, so it is difficult to establish regeneration systems for most of woody plants. Thus, those factors restricted the research progress in genetic improvement and related functional genes of woody plants by using the biotechnology. Isolated microspore culture, as an important way to obtain haploid, which could generate homozygous diploid plant after induction culture, not only speeds up the genetic improvement of woody plants, but also provides stable research materials for seedling breeding,molecular biology, genetics and morphological genology of woody plants. In this review, we mainly introduced the basic steps and the key factors influencing the success in microspore culture of woody plants, the research status and existing problems, and prospected the application foreground of the technology in woody plants in the future.
引文
Bueno M.A.,Pintos B.,Hofer M.,and Martin A.,2005,Proembryos induction from Olea europaea L.isolated microspore culture,Acta Physiol.Plant.,27(4):695-701
Chang K.J.,Tan D.G.,Li D.Q.,Zhen P.,and Zhang J.M.,2009,Applications of plant isolated microspores and microsporederived tissues in genetic transformation,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),25(15):16-21(常凯军,谭德冠,李定琴,郑鹏,张家明,2009,植物游离小孢子及其培养所获得的组织在遗传转化中的应用,中国农学通报,25(15):16-21)
Chen J.M.,2003,The exploitation of wild rare plant Nauclea officinalis,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),22(4):38-39(陈健妙,2003,珍稀野生植物乌檀的开发利用,中国野生植物资源,22(4):38-39)
Chen J.M.,Liu L.,Liu Z.P.,Long X.H.,Zhen Q.S.,and Mao Y.Q.,2009,Comparison of seed oil physicochemical characteristics among three cultivars of Jatropha curcas L.,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),20(12):2884-2890(陈健妙,刘联,刘兆普,隆小华,郑青松,毛轶清,2009,三种麻疯树种子油脂理化特性比较,应用生态学报,20(12):2884-2890)
Dong L.G.,and Ding Y.L.,2017,Development of micro-and mega-spores and morphology of floral organ in Osmanthus fragrans'zao zi yin',Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),25(3):299-308(董立格,丁雨龙,2017,‘早籽银桂’大小孢子发育与花器形态变化,热带亚热带植物学报,25(3):299-308)
Herrera J.C.,Moreno L.G.,Acuna J.R.,Pena M.D.,and Osorio D.,2002,Colchicine-induced microspore embryogenesis in coffee,Plant Cell Tiss.Org.Cult.,71(1):89-92
Hoekstra S.,Zijderveld M.H.V.,Louwerse J.D.,Heidekamp F.,and van der Mark F.,1992,Anther and microspore culture of Hordeum vulgare L.cv.Igri,Plant Sci.,86(1):89-96
H觟fer M.,2004,In vitro androgenesis in apple-improvement of the induction phase,Plant Cell Rep.,22(6):365-370
H觟fer M.,Touraev A.,and Heberle-Bors E.,1999,Induction of embryogenesis from isolated apple microspores,Plant Cell Rep.,18(12):1012-1017
Hu C.X.,and Su X.L.,2003,Research situation and prospect of botanical pesticide insecticide Melia azedarach in China,Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),15(3):52-56(胡长效,苏新林,2003,我国植物质农药苦楝研究概况与展望,江西农业学报,15(3):52-56)
Hu T.,and Kasha K.J.,1999,A cytological study of pretreatments used to improve isolated microspore cultures of wheat(Triticum aestivum L.)cv.Chris,Genome,42(3):432-441
Hui Q.D.,and Wang J.F.,2012,Resources of the main woody medicinal plant in Jiangsu Provincee,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),31(1):59-61(惠秋娣,王久粉,2012,江苏省主要木本药用植物资源,中国野生植物资源,31(1):59-61)
Lichter R.,1982,Induction of haploid plants from isolated pollen of Brassica napus,Zeitschrift für Pflanzenphysiologie,105(5):427-434
Min Z.Y.,Dong L.,Li L.,Tang Y.Y.,Xiao L.,Li H.,and Sun X.W.,2013,Research progress on isolated microspore culture of Cucurbitaceous vegetables,Changjiang Shucai(Journal of Changjiang Vegetables),(12):6-9(闵子扬,童龙,李玲,王志伟,唐炎英,肖璐,李涵,孙小武,2013,葫芦科蔬菜游离小孢子培养研究进展,长江蔬菜,(12):6-9)
Pedroso M.C.,and Pais M.S.,1994,Induction of microspore embryogenesis in Camellia japonica cv.Elegans,Plant Cell Tiss.Org.Cult.,37(2):129-136
Qiao L.T.,and Gao J.,2013,Studies on relationship between size of flower buds and uninucleate microspore at periphery in Brassica rapa L.,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),50(4):620-624(乔丽桃,高杰,2013,芜菁花蕾长度与花粉小孢子单核靠边期关系的研究,新疆农业科学,50(4):620-624)
Siebel J.,and Pauls K.P.,1989,A comparison of anther and microspore culture as a breeding tool in Brassica napus,Theor.Appl.Genet.,78(4):473-479
Song Y.Q.,Yao B.H.,Zhang Z.L.,Tian C.F.,and Li L.L.,2012,Studies on microsporogenesis and its morphology change in'Niitaka'pear(Pyrus pyrifolia),Guoshu Xuebao(Journal of Fruit Science),29(6):1027-1030(宋宇琴,姚宝花,张绍铃,田彩芳,李六林,2012,‘新高’梨小孢子的发生及其形态变化的研究,果树学报,29(6):1027-1030)
Tan D.G.,Wu Y.,Sun X.P.,Fu L.L.,Ma S.,and Zhang J.M.,2011,Isolated microspore culture in Hevea brasiliensis Müll.Arg,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),32(5):840-844(谭德冠,吴煜,孙雪飘,付莉莉,马帅,张家明,2011,巴西橡胶树游离小孢子培养,热带作物学报,32(5):840-844)
Tang B.,Tao L.,Yang J.,Wu K.Y.,Zhang L.,and Deng Y.,2017,Optimization of high frequency embryo induction system for isolated microspore culture in Chinese cabbage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),38(10):1913-1920(唐兵,陶莲,卢松,杨静,吴康云,张丽,邓英,2017,白菜游离小孢子培养高频胚诱导技术体系优化,热带作物学报,38(10):1913-1920)
Touraev A.,Pfosser M.,and Heberle-Bors E.,2001,The microspore:a haploid multipurpose cell,Adv.Bot.Res.,35:53-109
Wang L.X.,Lin Z.L.,Bai X.G.,Lv G.L.,Fu J.,Yin F.Y.,Huang X.Q.,and Chen Z.Q.,2009,Recent advance in isolated microspores cultivation of rice,Shengwu Jishu(Biotechnology),19(6):92-95(王玲仙,蔺忠龙,白现广,吕广磊,付坚,殷富有,黄兴奇,程在全,2009,水稻游离小孢子培养最新研究进展,生物技术,19(6):92-95)
Wang S.S.,2008,Study on induction technique of microspore development and high embryo rate in microspore culture in cabbage(Brassica oleracea.var.capitata L.),Thesis for M.S.,Northwest A&F University,Supervisor:Zhang E.H.,pp.32-33(王莎莎,2008,甘蓝小孢子发育观察与小孢子培养中高出胚率的诱导技术研究,硕士学位论文,西北农林科技大学,导师:张恩慧,pp.32-33)
Wang Y.F.,Lu R.J.,and Huang J.H.,2014,Review and perspective on the application of anther and microspore culture in barley breeding,Zhiwu Shengli Xuebao(Plant Physiology Journal),50(12):1753-1758(王亦菲,陆瑞菊,黄剑华,2014,花药和小孢子培养技术应用于大麦育种的回顾与展望,植物生理学报,50(12):1753-1758)
Wei X.Y.,Zhang Q.K.,Jia M.J.,Hu F.,and Rao C.M.,2010,Microspore culture of Brassica napus L.in season and counter-season,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(4):731-734(韦献雅,张其坤,贾明娟,胡芳,饶春梅,2010,甘蓝型油菜正季与反季材料小孢子的培养,基因组学与应用生物学,29(4):731-734)
Yang Z.L.,Zeng X.Q.,Chen F.F.,and Li G.Y.,2017,Status of Camellia oleifera resources in Hainan Island,Jingjilin Yanjiu(Nonwood Forest Research),35(4):226-230(杨枝林,曾祥全,陈飞飞,黎国运,2017,海南岛油茶资源果实经济性状评价,经济林研究,35(4):226-230)
Yu W.J.,Hou X.L.,Zhao X.M.,and Liu Z.K.,2013,Isolated microspore culture and plant regeneration in Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee,Nanjing Nongye Daxue Xuebao(Journal of Nanjing Agricultural University),36(2):7-12(于文佳,侯喜林,赵晓嫚,刘照坤,2013,小菘菜游离小孢子培养和植株再生,南京农业大学学报,36(2):7-12)
Zeng X.L.,Fang S.G.,Zhu C.H.,and Zhong K.Q.,2014,Isolated microspore culture and plant regeneration in flowering Chinese cabbage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),35(12):2397-2402(曾小玲,方淑桂,朱朝辉,钟开勤,2014,不同基因型菜心游离小孢子培养和植株再生,热带作物学报,35(12):2397-2402)
Zhan Y.,Chen J.F.,and Malik A.A.,2009,Embryoid induction and plant regeneration of cucumber(Cucumis sativus L.)through microspore culture,Yuanyi Xuebao(Acta Horticulturae Sinica),36(2):221-226(詹艳,陈劲枫,Malik A.A.,2009,黄瓜游离小孢子培养诱导成胚和植株再生,园艺学报,36(2):221-226)
Zhao X.M.,2013,Study on the factors affecting microspore embryogenesis and quality of broccoli,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Li Y.,pp.22-23(赵晓嫚,2013,青花菜小孢子胚胎发生及品质影响因素研究,硕士学位论文,南京农业大学,导师:李英,pp.22-23)
Zhou J.,Jiang L.J.,Li C.Z.,and Ma Q.,2011,Relativity between Cornus wilsoniana microsporogenesis period and the form of floral organ'and morphologic observation of pollen grain,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),31(7):109-114(周娟,蒋丽娟,李昌珠,马倩,2011,光皮树小孢子发育与花器形态相关性及花粉粒超微结构观察,中南林业科技大学学报,31(7):109-114)
Chang K.J.,Tan D.G.,Li D.Q.,Zhen P.,and Zhang J.M.,2009,Applications of plant isolated microspores and microsporederived tissues in genetic transformation,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),25(15):16-21(常凯军,谭德冠,李定琴,郑鹏,张家明,2009,植物游离小孢子及其培养所获得的组织在遗传转化中的应用,中国农学通报,25(15):16-21)
Chen J.M.,2003,The exploitation of wild rare plant Nauclea officinalis,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),22(4):38-39(陈健妙,2003,珍稀野生植物乌檀的开发利用,中国野生植物资源,22(4):38-39)
Chen J.M.,Liu L.,Liu Z.P.,Long X.H.,Zhen Q.S.,and Mao Y.Q.,2009,Comparison of seed oil physicochemical characteristics among three cultivars of Jatropha curcas L.,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),20(12):2884-2890(陈健妙,刘联,刘兆普,隆小华,郑青松,毛轶清,2009,三种麻疯树种子油脂理化特性比较,应用生态学报,20(12):2884-2890)
Dong L.G.,and Ding Y.L.,2017,Development of micro-and mega-spores and morphology of floral organ in Osmanthus fragrans'zao zi yin',Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),25(3):299-308(董立格,丁雨龙,2017,‘早籽银桂’大小孢子发育与花器形态变化,热带亚热带植物学报,25(3):299-308)
Herrera J.C.,Moreno L.G.,Acuna J.R.,Pena M.D.,and Osorio D.,2002,Colchicine-induced microspore embryogenesis in coffee,Plant Cell Tiss.Org.Cult.,71(1):89-92
Hoekstra S.,Zijderveld M.H.V.,Louwerse J.D.,Heidekamp F.,and van der Mark F.,1992,Anther and microspore culture of Hordeum vulgare L.cv.Igri,Plant Sci.,86(1):89-96
H觟fer M.,2004,In vitro androgenesis in apple-improvement of the induction phase,Plant Cell Rep.,22(6):365-370
H觟fer M.,Touraev A.,and Heberle-Bors E.,1999,Induction of embryogenesis from isolated apple microspores,Plant Cell Rep.,18(12):1012-1017
Hu C.X.,and Su X.L.,2003,Research situation and prospect of botanical pesticide insecticide Melia azedarach in China,Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),15(3):52-56(胡长效,苏新林,2003,我国植物质农药苦楝研究概况与展望,江西农业学报,15(3):52-56)
Hu T.,and Kasha K.J.,1999,A cytological study of pretreatments used to improve isolated microspore cultures of wheat(Triticum aestivum L.)cv.Chris,Genome,42(3):432-441
Hui Q.D.,and Wang J.F.,2012,Resources of the main woody medicinal plant in Jiangsu Provincee,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),31(1):59-61(惠秋娣,王久粉,2012,江苏省主要木本药用植物资源,中国野生植物资源,31(1):59-61)
Lichter R.,1982,Induction of haploid plants from isolated pollen of Brassica napus,Zeitschrift für Pflanzenphysiologie,105(5):427-434
Min Z.Y.,Dong L.,Li L.,Tang Y.Y.,Xiao L.,Li H.,and Sun X.W.,2013,Research progress on isolated microspore culture of Cucurbitaceous vegetables,Changjiang Shucai(Journal of Changjiang Vegetables),(12):6-9(闵子扬,童龙,李玲,王志伟,唐炎英,肖璐,李涵,孙小武,2013,葫芦科蔬菜游离小孢子培养研究进展,长江蔬菜,(12):6-9)
Pedroso M.C.,and Pais M.S.,1994,Induction of microspore embryogenesis in Camellia japonica cv.Elegans,Plant Cell Tiss.Org.Cult.,37(2):129-136
Qiao L.T.,and Gao J.,2013,Studies on relationship between size of flower buds and uninucleate microspore at periphery in Brassica rapa L.,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),50(4):620-624(乔丽桃,高杰,2013,芜菁花蕾长度与花粉小孢子单核靠边期关系的研究,新疆农业科学,50(4):620-624)
Siebel J.,and Pauls K.P.,1989,A comparison of anther and microspore culture as a breeding tool in Brassica napus,Theor.Appl.Genet.,78(4):473-479
Song Y.Q.,Yao B.H.,Zhang Z.L.,Tian C.F.,and Li L.L.,2012,Studies on microsporogenesis and its morphology change in'Niitaka'pear(Pyrus pyrifolia),Guoshu Xuebao(Journal of Fruit Science),29(6):1027-1030(宋宇琴,姚宝花,张绍铃,田彩芳,李六林,2012,‘新高’梨小孢子的发生及其形态变化的研究,果树学报,29(6):1027-1030)
Tan D.G.,Wu Y.,Sun X.P.,Fu L.L.,Ma S.,and Zhang J.M.,2011,Isolated microspore culture in Hevea brasiliensis Müll.Arg,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),32(5):840-844(谭德冠,吴煜,孙雪飘,付莉莉,马帅,张家明,2011,巴西橡胶树游离小孢子培养,热带作物学报,32(5):840-844)
Tang B.,Tao L.,Yang J.,Wu K.Y.,Zhang L.,and Deng Y.,2017,Optimization of high frequency embryo induction system for isolated microspore culture in Chinese cabbage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),38(10):1913-1920(唐兵,陶莲,卢松,杨静,吴康云,张丽,邓英,2017,白菜游离小孢子培养高频胚诱导技术体系优化,热带作物学报,38(10):1913-1920)
Touraev A.,Pfosser M.,and Heberle-Bors E.,2001,The microspore:a haploid multipurpose cell,Adv.Bot.Res.,35:53-109
Wang L.X.,Lin Z.L.,Bai X.G.,Lv G.L.,Fu J.,Yin F.Y.,Huang X.Q.,and Chen Z.Q.,2009,Recent advance in isolated microspores cultivation of rice,Shengwu Jishu(Biotechnology),19(6):92-95(王玲仙,蔺忠龙,白现广,吕广磊,付坚,殷富有,黄兴奇,程在全,2009,水稻游离小孢子培养最新研究进展,生物技术,19(6):92-95)
Wang S.S.,2008,Study on induction technique of microspore development and high embryo rate in microspore culture in cabbage(Brassica oleracea.var.capitata L.),Thesis for M.S.,Northwest A&F University,Supervisor:Zhang E.H.,pp.32-33(王莎莎,2008,甘蓝小孢子发育观察与小孢子培养中高出胚率的诱导技术研究,硕士学位论文,西北农林科技大学,导师:张恩慧,pp.32-33)
Wang Y.F.,Lu R.J.,and Huang J.H.,2014,Review and perspective on the application of anther and microspore culture in barley breeding,Zhiwu Shengli Xuebao(Plant Physiology Journal),50(12):1753-1758(王亦菲,陆瑞菊,黄剑华,2014,花药和小孢子培养技术应用于大麦育种的回顾与展望,植物生理学报,50(12):1753-1758)
Wei X.Y.,Zhang Q.K.,Jia M.J.,Hu F.,and Rao C.M.,2010,Microspore culture of Brassica napus L.in season and counter-season,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(4):731-734(韦献雅,张其坤,贾明娟,胡芳,饶春梅,2010,甘蓝型油菜正季与反季材料小孢子的培养,基因组学与应用生物学,29(4):731-734)
Yang Z.L.,Zeng X.Q.,Chen F.F.,and Li G.Y.,2017,Status of Camellia oleifera resources in Hainan Island,Jingjilin Yanjiu(Nonwood Forest Research),35(4):226-230(杨枝林,曾祥全,陈飞飞,黎国运,2017,海南岛油茶资源果实经济性状评价,经济林研究,35(4):226-230)
Yu W.J.,Hou X.L.,Zhao X.M.,and Liu Z.K.,2013,Isolated microspore culture and plant regeneration in Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee,Nanjing Nongye Daxue Xuebao(Journal of Nanjing Agricultural University),36(2):7-12(于文佳,侯喜林,赵晓嫚,刘照坤,2013,小菘菜游离小孢子培养和植株再生,南京农业大学学报,36(2):7-12)
Zeng X.L.,Fang S.G.,Zhu C.H.,and Zhong K.Q.,2014,Isolated microspore culture and plant regeneration in flowering Chinese cabbage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),35(12):2397-2402(曾小玲,方淑桂,朱朝辉,钟开勤,2014,不同基因型菜心游离小孢子培养和植株再生,热带作物学报,35(12):2397-2402)
Zhan Y.,Chen J.F.,and Malik A.A.,2009,Embryoid induction and plant regeneration of cucumber(Cucumis sativus L.)through microspore culture,Yuanyi Xuebao(Acta Horticulturae Sinica),36(2):221-226(詹艳,陈劲枫,Malik A.A.,2009,黄瓜游离小孢子培养诱导成胚和植株再生,园艺学报,36(2):221-226)
Zhao X.M.,2013,Study on the factors affecting microspore embryogenesis and quality of broccoli,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Li Y.,pp.22-23(赵晓嫚,2013,青花菜小孢子胚胎发生及品质影响因素研究,硕士学位论文,南京农业大学,导师:李英,pp.22-23)
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