PEG模拟干旱条件下木麻黄幼苗的生理变化
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摘要
以木麻黄Casuarina equisetifolia15个家系品种为材料,利用聚乙二醇6000(PEG-6000)模拟水分胁迫环境,设置三个胁迫强度处理(轻度、中度、重度胁迫),研究受胁迫2月龄木麻黄幼苗丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、脯氨酸含量的变化。结果表明,经干旱胁迫处理后,各家系幼苗叶片MDA含量随胁迫强度增大而增大,但增幅差异较大;不同干旱胁迫水平下,各家系SOD活性存在一定差异;重度胁迫条件下,除粤杂交家系外,其余家系脯氨酸含量与对照相比均为显著增加。采用主成分分析法进行抗旱性综合评价,表明MDA含量是反映水分胁迫最重要的指标。各指标综合分析结果表明,家系701、莆20、抗3、惠2、南山7和东山2抗旱性能力较好;东山短、601、59、A13抗旱能力一般;千头、粤杂交、抗1、龙4和平5抗旱能力较差。
The two month old seedlings of 15 families of Casuarina equisetifolia were treated with different concentrations of polyethylene glycol-6000(PEG-6000) to study the changes of malondialdehyde(MDA), superoxide dismutase(SOD) activity and proline content. Results indicated that MDA content of seedling leaves of each family increased with the increase of stress intensity after drought stress treatment, but the increase rate of each family was quite different. The SOD activity of each family was different under different drought stress levels, and showed different capacity response to drought resistance. With the severe stress treatment, except the Yue-hybrid, the proline content of other families increased significantly compared with control. The comprehensive evaluation of drought resistance by principal component analysis showed that MDA content was the most important index reflecting water stress. Based on comprehensive analysis, the drought-resistance of 701, Pu2, Kang3, Hui2, Nanshan7, Dongshan2 were strong, the drought-resistance of Dongshanduan, 601, 59, A13 were middle, but the drought-resistance of Yue-hybrid, Kang1, Long4, Ping5 were weak.
The two month old seedlings of 15 families of Casuarina equisetifolia were treated with different concentrations of polyethylene glycol-6000(PEG-6000) to study the changes of malondialdehyde(MDA), superoxide dismutase(SOD) activity and proline content. Results indicated that MDA content of seedling leaves of each family increased with the increase of stress intensity after drought stress treatment, but the increase rate of each family was quite different. The SOD activity of each family was different under different drought stress levels, and showed different capacity response to drought resistance. With the severe stress treatment, except the Yue-hybrid, the proline content of other families increased significantly compared with control. The comprehensive evaluation of drought resistance by principal component analysis showed that MDA content was the most important index reflecting water stress. Based on comprehensive analysis, the drought-resistance of 701, Pu2, Kang3, Hui2, Nanshan7, Dongshan2 were strong, the drought-resistance of Dongshanduan, 601, 59, A13 were middle, but the drought-resistance of Yue-hybrid, Kang1, Long4, Ping5 were weak.
引文
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[13]何开跃,李晓储,黄利斌,张永兵,胡晓健.干旱胁迫对木兰科5树种生理生化指标的影响[J].植物资源与环境学报,2004,13(4):20-23.
[14]陈立松,刘景辉.水分胁迫对荔枝叶片活性氧代谢的影响[J].园艺学报,1998,25(3):241-246.
[15]Arndt S K,Clifford S C,Wanek W.Physiological and morphological adaptation of the fruit tree Ziziphus rotundifolia in response to progressive drought stress[J].Tree Physiology,2001,21:705-715.
[16]李燕,孙明高,孔艳菊,薛立.皂角苗木对干旱胁迫的生理生化反应[J].华南农业大学学报,2006,27(3):66-69.
[17]赵琳,郎南军,温绍龙,郑科.云南干热河谷4种植物抗旱机理的研究[J].西部林业科学,2005,35(2):9-16.
[18]黄金塔.干旱胁迫下木麻黄15个无性系幼苗生理指标的变化[J].防护林科技,2011(4):32-35.
[19]黎燕琼,郑绍伟,陈泓,刘军,吴永波.林木抗旱性研究及其进展[J].世界林业研究,2007,20(1):10-15.
[20]冯慧芳,薛立,任向荣,傅静丹,郑卫国.4种阔叶幼苗对PEG模拟干旱的生理响应[J].生态学报,2011,31(2):371-382.
[21]德英,赵来喜,穆怀彬.PEG-6000渗透胁迫下应用电导法对披碱草属种质幼苗抗旱性初步研究[J].中国农学通报,2010,26(24):173-178.
[22]李凤珍,马晓岗.PEG处理下青海栽培小麦萌发期及幼苗抗旱性研究[J].中国农学通报,2011,27(21):44-48.
[2]仲崇禄,张勇.我国木麻黄的引种培育和经营[J].林业科技开发,2003,17(2):3-5.
[3]聂森,张勇,仲崇禄,叶功富.福建沿海木麻黄速生抗性无性系选育[J].福建林学院学报,2014,32(4):300-304.
[4]李茂谨.短枝木麻黄国际种源试验和早期选择[J].热带亚热带植物学报,2018,26(5):597-603.
[5]武冲,张勇,唐树梅,仲崇禄.盐胁迫对木麻黄种子萌发的影响[J].种子,2010,29(4):30-32.
[6]张水松,谭芳林,林武星,陈胜,李永林.海岸带风口沙地木麻黄无性系造林与根系生长特性[J].林业科学,2005,41(1):85-90.
[7]高琼,陈晓阳,杜金友,郝霞.不同种和种源胡枝子的耐旱性差异研究[J].北华大学学报(自然科学版),2005,6(3):257-260.
[8]韩蕊莲,李丽霞,梁宗锁,薛智德,杜正科.干旱胁迫下沙棘膜脂过氧化保护体系研究[J].西北林学院学报,2002,17(4):1-5.
[9]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:115-116.
[10]杨建伟,韩蕊莲,刘淑明,梁宗锁.不同土壤水分下杨树的蒸腾变化及抗旱适应性研究[J].西北林学院学报,2004,19(3):7-10.
[11]黄少伟,谢维辉.实用SAS编程与林业试验数据分析[M].广州:华南理工大学出版社,2001:171-177.
[12]李志刚,刘威,林彰文,顾继光.芦笋在PEG模拟干旱条件下的生理生化变化[J].生态科学,2006,25(1):21-24.
[13]何开跃,李晓储,黄利斌,张永兵,胡晓健.干旱胁迫对木兰科5树种生理生化指标的影响[J].植物资源与环境学报,2004,13(4):20-23.
[14]陈立松,刘景辉.水分胁迫对荔枝叶片活性氧代谢的影响[J].园艺学报,1998,25(3):241-246.
[15]Arndt S K,Clifford S C,Wanek W.Physiological and morphological adaptation of the fruit tree Ziziphus rotundifolia in response to progressive drought stress[J].Tree Physiology,2001,21:705-715.
[16]李燕,孙明高,孔艳菊,薛立.皂角苗木对干旱胁迫的生理生化反应[J].华南农业大学学报,2006,27(3):66-69.
[17]赵琳,郎南军,温绍龙,郑科.云南干热河谷4种植物抗旱机理的研究[J].西部林业科学,2005,35(2):9-16.
[18]黄金塔.干旱胁迫下木麻黄15个无性系幼苗生理指标的变化[J].防护林科技,2011(4):32-35.
[19]黎燕琼,郑绍伟,陈泓,刘军,吴永波.林木抗旱性研究及其进展[J].世界林业研究,2007,20(1):10-15.
[20]冯慧芳,薛立,任向荣,傅静丹,郑卫国.4种阔叶幼苗对PEG模拟干旱的生理响应[J].生态学报,2011,31(2):371-382.
[21]德英,赵来喜,穆怀彬.PEG-6000渗透胁迫下应用电导法对披碱草属种质幼苗抗旱性初步研究[J].中国农学通报,2010,26(24):173-178.
[22]李凤珍,马晓岗.PEG处理下青海栽培小麦萌发期及幼苗抗旱性研究[J].中国农学通报,2011,27(21):44-48.