桂枝茯苓胶囊耐缺血缺氧能力的实验研究
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摘要
目的探讨桂枝茯苓胶囊对缺血缺氧耐受力的影响。方法按体质量将小鼠随机分为空白对照组、阳性药物对照组(普萘洛尔)、桂枝茯苓胶囊大剂量组(0.93g/kg)、桂枝茯苓胶囊小剂量组(0.465g/kg),连续给药7d,采用密闭缺氧实验、断头致脑缺氧实验、亚硝酸钠致脑缺氧实验以及异丙肾上腺素致脑缺氧实验,记录小鼠耐缺氧存活时间;建立异丙肾上腺素致大鼠心肌缺血缺氧模型,并以桂枝茯苓胶囊进行治疗,探讨其对缺血缺氧的影响。结果与空白对照组相比,大剂量桂枝茯苓胶囊对密闭导致的缺氧存活时间和大、小剂量桂枝茯苓胶囊对断头致脑缺氧存活时间有延长趋势(P>0.05);小剂量桂枝茯苓胶囊对亚硝酸钠导致的缺氧存活时间、大剂量桂枝茯苓胶囊对异丙肾上腺素导致的缺氧存活时间具有显著延长作用(P<0.05)。与模型对照组相比,大、小剂量桂枝茯苓胶囊对异丙肾上腺素致大鼠心肌缺血缺氧坏死面积有显著降低作用(P<0.05)。结论桂枝茯苓胶囊对异丙肾上腺素导致的大鼠心肌缺血缺氧具有显著的保护作用。
Objective To investigate the protective effect of Gui Zhi Fuling Jiaonang on endurance to ischemia and hypoxia.Methods Mice were randomized into four groups:control group,positive drug control group,Gui Zhi Fuling Jiaonang high-dose group(0.93g/kg),and Gui Zhi Fuling Jiaonang low-dose group(0.465g/kg).All mice were treated with corresponding drugs for 7 days.The hypoxia mice model was established through hypoxia in the closed jars,cerebral anoxia by decapitation,poisoning with sodium nitrite and isoprenaline.Then the hypoxia-ischemia rat model was established by injecting isoproterenol.The anti-hypoxic effects were observed.Results Compared with control group,Gui Zhi Fuling Jiaonang high-dose group(0.93 g/kg)had a tendency to extend the survival time of mice model established through hypoxia in the closed jars;Gui Zhi Fuling Jiaonang highdose(0.93 g/kg)and low-dose(0.465 g/kg)groups had a tendency to extend the survival time of mice model established through cerebral anoxia by decapitation(P>0.05).Compared with that in control group,the survival time of mice in Gui Zhi Fuling Jiaonang low-dose group under poisoning with sodium nitrite and Gui Zhi Fuling Jiaonang high-dose group under poisoning with isoprenaline were significantly prolonged.Besides,Gui Zhi Fuling Jiaonang relieved myocardial tissue damage caused by ischemia and hypoxia(P<0.05).Conclusion Gui Zhi Fuling Jiaonang has an obviously protective effect on isoprenaline-induced hypoxia and myocardial ischemia.
Objective To investigate the protective effect of Gui Zhi Fuling Jiaonang on endurance to ischemia and hypoxia.Methods Mice were randomized into four groups:control group,positive drug control group,Gui Zhi Fuling Jiaonang high-dose group(0.93g/kg),and Gui Zhi Fuling Jiaonang low-dose group(0.465g/kg).All mice were treated with corresponding drugs for 7 days.The hypoxia mice model was established through hypoxia in the closed jars,cerebral anoxia by decapitation,poisoning with sodium nitrite and isoprenaline.Then the hypoxia-ischemia rat model was established by injecting isoproterenol.The anti-hypoxic effects were observed.Results Compared with control group,Gui Zhi Fuling Jiaonang high-dose group(0.93 g/kg)had a tendency to extend the survival time of mice model established through hypoxia in the closed jars;Gui Zhi Fuling Jiaonang highdose(0.93 g/kg)and low-dose(0.465 g/kg)groups had a tendency to extend the survival time of mice model established through cerebral anoxia by decapitation(P>0.05).Compared with that in control group,the survival time of mice in Gui Zhi Fuling Jiaonang low-dose group under poisoning with sodium nitrite and Gui Zhi Fuling Jiaonang high-dose group under poisoning with isoprenaline were significantly prolonged.Besides,Gui Zhi Fuling Jiaonang relieved myocardial tissue damage caused by ischemia and hypoxia(P<0.05).Conclusion Gui Zhi Fuling Jiaonang has an obviously protective effect on isoprenaline-induced hypoxia and myocardial ischemia.
引文
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[12]刘昊琛,邓忠,张忆雯,等.乙醇致家兔急性心衰模型的建立[J].西安交通大学学报(医学版),2012,33(4):435-438.
[13]GRIMM D,ELSNER D,SCHUNKERT H,et al.Development of heart failure following isoproterenol administration in the rat:role of the renin-angiotensin system[J].Cardiovasc Res,1998,37(1):91-100.
[14]刘新岩.枸杞多糖对异丙肾上腺素致心力衰竭大鼠的保护作用及其机制[J].宁夏医科大学学报,2011,33(7):601-604.
[2]李佳川,刘朋,刘晓帅,等.桂枝茯苓丸对垂体后叶素致大鼠急性心肌缺血的保护作用[J].西南民族大学学报(自然科学版),2013,39(3):327-329.
[3]杨学斌.浅析桂枝茯苓丸治疗脑血管病[J].光明中医,2008,23(8):1166-1167.
[4]陈主初.病理生理学[M].北京:人民卫生出版社,2005.152-165.
[5]张汝学,贾正平,李富盛,等.淫羊藿总黄酮对缺氧模型小鼠的抗缺氧作用[J].中药材,2009,11(32):1736-1738.
[6]WEST JB.The physiologic basis of high-altitude diseases[J].Ann Intern Med,2004,141(10):789-800.
[7]邹丽琰,傅延龄.桂枝蒸馏液对离体灌流鼠心缺血/再灌注损伤的保护作用[J].北京中医药大学学报,1997,20(2):22.
[8]徐幕鸽,魏凯峰.桂枝活血机理及配伍规律探析[J].中医药导报,2013,19(8):12-14.
[9]李永芳,杨梅,寇毅英,等.八味沉香散对异丙肾上腺素诱导的大鼠心肌缺血损伤的保护作用[J].中药药理与临床,2008,24(2):63-65.
[10]LU L,WU W,YAN J,et al.Adriamycin-induced autophagic cardiomyocyte death plays a pathogenic role in a rat model of heart failure[J].Int J Cardiol,2009,134(1):82-90.
[11]CHRISTIANSEN S,REDMANN K,SCHELD HH,et al.Adriamycin-induced cardiomyopathy in the dog—an appropriate model for research on partial left ventriculectomy?[J].J Heart Lung Transpl,2002,21(7):783-790.
[12]刘昊琛,邓忠,张忆雯,等.乙醇致家兔急性心衰模型的建立[J].西安交通大学学报(医学版),2012,33(4):435-438.
[13]GRIMM D,ELSNER D,SCHUNKERT H,et al.Development of heart failure following isoproterenol administration in the rat:role of the renin-angiotensin system[J].Cardiovasc Res,1998,37(1):91-100.
[14]刘新岩.枸杞多糖对异丙肾上腺素致心力衰竭大鼠的保护作用及其机制[J].宁夏医科大学学报,2011,33(7):601-604.