DNA甲基化转移酶1与2,3,7,8-四氯二苯并对二噁英诱导小鼠腭裂的相关性研究
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摘要
目的:通过检测DNA甲基转移酶1(DNA methyltransferase 1,DNMT1)在四氯二苯并对二噁英(2,3,7,8-tetrachlorodibenzo-paradioxin,TCDD)诱导的腭裂小鼠体内的表达,探讨其与腭裂发病机制的相关性。方法:TCDD通过对腭部中嵴上皮细胞的毒性作用,导致胎鼠腭裂发生。应用TCDD(64μg/kg)处理胚胎处于第10.5天(E10.5)的C57BL/6J怀孕小鼠建模。分别获取E13.5、E14.5、E15.5和E17.5胎鼠的口腔组织,苏木精-伊红(hematoxylin-eosin staining,HE)染色检测胎鼠腭裂组织。逆转录-定量聚合酶链反应(reverse transcription-quantitative polymerase chain reaction,RT-qPCR)检测以上各组胎鼠DNMT1mRNA表达水平的变化,Western blot检测DNMT1蛋白表达水平的变化。结果:HE染色结果显示TCDD组腭裂发生率为98.25%,对照组无腭裂发生。RTqPCR结果显示TCDD组DNMT1mRNA水平与对照组比较显著增高,差异有统计学意义(P<0.01)。Western blot结果表明与对照组比较,TCDD组DNMT1蛋白表达水平显著增高,差异有统计学意义(P<0.01)。结论:在TCDD诱导胎鼠腭裂的形成过程中,DNMT1发挥重要的作用。
Objective:To investigate the correlation between DNMT1 and TCDD-induced cleft palate in mice by detecting the expression of DNMT1 in TCDD-induced cleft palate mice.Methods:TCDD could cause fetus rats cleft palate by toxic effects on palatal medial edge epithelial cells.C57BL/6Jpregnant mice were treated with TCDD(64μg/kg)at day 10.5(E10.5)to be the modeling.Oral tissues of E13.5,E14.5,E15.5,and E17.5fetal rats were obtained,and HE staining was used to detect the occurrence of fetal cleft palate.Reverse transcription quantitative polymerase chain reaction was used to detect the changes of mRNA expression in DNMT1 of fetal rats.Western blot was used to detect the expression level of DNMT1 protein.Results:HE staining showed that the incidence of cleft palate in group TCDD was 56/57,and there was no cleft palate in control group.RT-qPCR results showed that DNMT1 mRNA in TCDD group was significantly higher than that in control group(P<0.01).Western blot results showed that compared with the control group,the expression level of DNMT1 protein in TCDD group was significantly higher(P<0.01).Conclusion:Methyltransferase DNMT1 plays an important role in the process of TCDD induced cleft palate formation in fetal rats.
Objective:To investigate the correlation between DNMT1 and TCDD-induced cleft palate in mice by detecting the expression of DNMT1 in TCDD-induced cleft palate mice.Methods:TCDD could cause fetus rats cleft palate by toxic effects on palatal medial edge epithelial cells.C57BL/6Jpregnant mice were treated with TCDD(64μg/kg)at day 10.5(E10.5)to be the modeling.Oral tissues of E13.5,E14.5,E15.5,and E17.5fetal rats were obtained,and HE staining was used to detect the occurrence of fetal cleft palate.Reverse transcription quantitative polymerase chain reaction was used to detect the changes of mRNA expression in DNMT1 of fetal rats.Western blot was used to detect the expression level of DNMT1 protein.Results:HE staining showed that the incidence of cleft palate in group TCDD was 56/57,and there was no cleft palate in control group.RT-qPCR results showed that DNMT1 mRNA in TCDD group was significantly higher than that in control group(P<0.01).Western blot results showed that compared with the control group,the expression level of DNMT1 protein in TCDD group was significantly higher(P<0.01).Conclusion:Methyltransferase DNMT1 plays an important role in the process of TCDD induced cleft palate formation in fetal rats.
引文
[1]傅夏洲.中国中部人群非综合征型唇腭裂的遗传学分析[D].武汉大学,2014.
[2]邱蔚六.口腔颌面外科学[M].北京:人民卫生出版社,2006.360-363.
[3]张芳真,郑苍尚,邓永强,等.1320例唇腭裂患者临床统计分析[J].口腔医学研究,2008,24(1):82-84.
[4]多力昆·吾尔,木合塔尔·霍加,米热古丽·吾马尔,等.215例维吾尔族唇腭裂患者的回顾性分析[J].国际口腔医学杂志,2013,40(5):580-582.
[5] Beaty TH,Ruczinski I,Murray JC,et al.Evidence for geneenvironment interaction in a genome wide study of nonsyndromic cleft palate[J].Genet Epidemiol,2011,35(6):469-478.
[6] Msoure JA,Gupta BN,Zinkl JG,et al.Postnatal effects of maternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)[J].Environ Health Perspect,1973,5:81-85.
[7] Liu X,Qi J,Tao Y,et al.Correlation of proliferation,TGF-β3promoter methylation,and Smad signaling in MEPM cells during the development of ATRA-induced cleft palate[J].Reprod Toxicol,2016,61:1-9.
[8] Jaenisch R,Bird A.Epigenetic regulation of geneexpression:how the genome integrates intrinsic andenvironmental signals[J].Nat Genet,2003,33Suppl:245-254.
[9] Chi P,Allis CD,Wang GG.Covalent histone modificationsmiswritten,misinterpreted and mis-erased in humancancers[J].Nat Rev Cancer,2010,10(7):457-469.
[10] Reid MA,Dai Z,Locasale JW.The impact of cellularmetabolism on chromatin dynamics and epigenetics[J].Nat Cell Biol,2017,19(11):1298-1306.
[11]何苗,范晶,林劼.等.DNA甲基转移酶与甲基化CPG结合结构蛋白2在胃肠间质瘤的表达[J].检验医学与临床,2016,13(4):469-471.
[12] Hermann A,Goyal R,Jeltsch A.The Dnmt1DNA-(cytosineC5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites[J].J Biol Chem,2004,279(46):48350-48359.
[13]苍松,焦晓辉,王丽,等.中国人群非综合征性唇腭裂TGFA基因TaqⅠ多态性研究[J].哈尔滨医科大学学报,2002,36(5):369-371.
[14] Shi J,Song T,Jiao X,et al.Single-nucleotide polymorphisms(SNPs)of the IRF6and TFAP2Ain non-syndromic cleft lip with orwithout cleft palate(NSCLP)in a northern Chinese population[J].Biochem Biophys Res Commun,2011,410(4):732-736.
[15]焦晓辉,王丽,徐旭光.中国人群非综合征性唇腭裂与TGFβ3多态性的关系[J].哈尔滨医科大学学报,2003,37(5):415-418.
[16]傅豫川,金辉喜,李小丹,等.MEE细胞在鼠腭发育及腭裂形成过程中的分化与转归[J].中国口腔颌面外科杂志,2003,1(4):234-238.
[17]汪淼,黄洪章,侯劲松.小鼠体外胚胎腭器官培养模型的建立及全反式维甲酸致畸机制的研究[J].中华口腔医学研究杂志(电子版),2010,4(3):215-222.
[18] Gan LQ,Fu YX,Liu X,et al.Transforming growth factorbeta3expressionup-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice[J].Toxicol Ind Health,2009,25(7):473-478.
[19] Nogai H,Rosowski M,Grün J,et al.Follistatin antagonizes transforming growth factor-beta3-induced epithelial-mesenchymal transition in vitro:implications for murine palatal development supported by microarray analysis[J].Differentiation,2008,76(4):404-416.
[20] Ahmed S,Liu CC,Nawshad A.Mechanisms of palatal epithelialseam disintegration by transforming growth factor(TGF)β3[J].Developmental Biology,2007,309(2):193-207.
[21]侯志军.p63基因甲基化与非综合征性唇腭裂的相关性研究[D].青岛大学,2017.
[2]邱蔚六.口腔颌面外科学[M].北京:人民卫生出版社,2006.360-363.
[3]张芳真,郑苍尚,邓永强,等.1320例唇腭裂患者临床统计分析[J].口腔医学研究,2008,24(1):82-84.
[4]多力昆·吾尔,木合塔尔·霍加,米热古丽·吾马尔,等.215例维吾尔族唇腭裂患者的回顾性分析[J].国际口腔医学杂志,2013,40(5):580-582.
[5] Beaty TH,Ruczinski I,Murray JC,et al.Evidence for geneenvironment interaction in a genome wide study of nonsyndromic cleft palate[J].Genet Epidemiol,2011,35(6):469-478.
[6] Msoure JA,Gupta BN,Zinkl JG,et al.Postnatal effects of maternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)[J].Environ Health Perspect,1973,5:81-85.
[7] Liu X,Qi J,Tao Y,et al.Correlation of proliferation,TGF-β3promoter methylation,and Smad signaling in MEPM cells during the development of ATRA-induced cleft palate[J].Reprod Toxicol,2016,61:1-9.
[8] Jaenisch R,Bird A.Epigenetic regulation of geneexpression:how the genome integrates intrinsic andenvironmental signals[J].Nat Genet,2003,33Suppl:245-254.
[9] Chi P,Allis CD,Wang GG.Covalent histone modificationsmiswritten,misinterpreted and mis-erased in humancancers[J].Nat Rev Cancer,2010,10(7):457-469.
[10] Reid MA,Dai Z,Locasale JW.The impact of cellularmetabolism on chromatin dynamics and epigenetics[J].Nat Cell Biol,2017,19(11):1298-1306.
[11]何苗,范晶,林劼.等.DNA甲基转移酶与甲基化CPG结合结构蛋白2在胃肠间质瘤的表达[J].检验医学与临床,2016,13(4):469-471.
[12] Hermann A,Goyal R,Jeltsch A.The Dnmt1DNA-(cytosineC5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites[J].J Biol Chem,2004,279(46):48350-48359.
[13]苍松,焦晓辉,王丽,等.中国人群非综合征性唇腭裂TGFA基因TaqⅠ多态性研究[J].哈尔滨医科大学学报,2002,36(5):369-371.
[14] Shi J,Song T,Jiao X,et al.Single-nucleotide polymorphisms(SNPs)of the IRF6and TFAP2Ain non-syndromic cleft lip with orwithout cleft palate(NSCLP)in a northern Chinese population[J].Biochem Biophys Res Commun,2011,410(4):732-736.
[15]焦晓辉,王丽,徐旭光.中国人群非综合征性唇腭裂与TGFβ3多态性的关系[J].哈尔滨医科大学学报,2003,37(5):415-418.
[16]傅豫川,金辉喜,李小丹,等.MEE细胞在鼠腭发育及腭裂形成过程中的分化与转归[J].中国口腔颌面外科杂志,2003,1(4):234-238.
[17]汪淼,黄洪章,侯劲松.小鼠体外胚胎腭器官培养模型的建立及全反式维甲酸致畸机制的研究[J].中华口腔医学研究杂志(电子版),2010,4(3):215-222.
[18] Gan LQ,Fu YX,Liu X,et al.Transforming growth factorbeta3expressionup-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice[J].Toxicol Ind Health,2009,25(7):473-478.
[19] Nogai H,Rosowski M,Grün J,et al.Follistatin antagonizes transforming growth factor-beta3-induced epithelial-mesenchymal transition in vitro:implications for murine palatal development supported by microarray analysis[J].Differentiation,2008,76(4):404-416.
[20] Ahmed S,Liu CC,Nawshad A.Mechanisms of palatal epithelialseam disintegration by transforming growth factor(TGF)β3[J].Developmental Biology,2007,309(2):193-207.
[21]侯志军.p63基因甲基化与非综合征性唇腭裂的相关性研究[D].青岛大学,2017.