全波长扫描式多功能读数仪-分光光度法测定土壤中无机氮
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摘要
快速准确测定土壤中铵态氮、硝态氮含量对监测土壤肥力水平和生态环境,指导作物氮肥施用非常重要。选择30份土样,利用全波长扫描式多功能读数仪(酶标仪)结合靛酚蓝分光光度法、硫酸肼还原法测定土壤中铵态氮和硝态氮含量,探讨利用酶标仪测定土壤无机氮含量的可行性。结果显示,利用酶标仪测定土壤铵态氮、硝态氮含量与连续流动分析仪测定结果之间无明显差异,彼此间呈显著线性相关。铵态氮回归直线方程为Y(连续流动分析仪-NH_4~+-N)=0.997 6 X(酶标仪-NH_4~+-N)-0.012 3,相关系数R=0.961 9(n=30,P<0.01);硝态氮回归方程为Y(连续流动分析仪-NO_3~--N)=0.959 3 X(酶标仪-NO_3~--N)+0.021 9,相关系数R=0.964 0(n=30,P<0.01)。酶标仪测定铵态氮回收率在96.2%～108%,相对标准偏差在10%以内;硝态氮测定回收率为94.9%～110%,且相对标准偏差在5%以内,酶标仪测定土壤铵态氮和硝态氮方法检出限分别为0.068mg/L和0.028mg/L。酶标仪测定土壤无机氮速度快,精密度、准确度较高,消耗试剂少,可用于大批量土壤浸提液中铵态氮和硝态氮含量的快速分析。
It is important to quickly and accurately determine the ammonium and nitrate content in the soil for assessing soil fertility and ecological environment and for directing fertilizer recommendation.In this paper,the contents of NH_4~+-N、NO_3~--N in soil were determined through a microplate reader with indophenol-blue spectrophotometry and barium sulfate reduction method,respectively.We selected 30 soil samples for determination and compared with that of AA3 in order to establish method for soil inorganic nitrogen determination.The results of NH_4~+-N and NO_3~--N determined by the microplate reader and the continuous flow analyzer were compared. Regression equation for NH_4~+-N was established as Y(Continuous flow analyzer-NH_4~+-N)=0.997 6 X(Microplate reader-NH_4~+-N)-0.012 3 with correlation coefficient R=0.961 9(n=30,P<0.01).Regression equation for NO_3~--N was Y(Continuous flow analyzer-NO_3~--N)=0.959 3 X(Microplate reader-NO_3~--N)+0.021 9 with correlation coefficient R=0.9640(n =30,P<0.01).The recovery rates of NH_4~+-N and NO_3~--N concentrations measured by the microplate reader were 96.2%—108% and 94.9%—110%,respectively.The relative standard deviation(RSD)of proposed colorimetric method with microplate reader was less than 10% and 5%(n=6)for NH_4~+-N and NO_3~-N,respectively.The detection limits of NH_4~+-N and NO_3~--N content by the microplate reader were 0.068 mg/L and 0.028 mg/L,respectively.It is suggested that microplate reader with colorimetric method can be used for rapid analysis of ammonium and nitrate nitrogen content in soil extracts with high accuracy and precision.
It is important to quickly and accurately determine the ammonium and nitrate content in the soil for assessing soil fertility and ecological environment and for directing fertilizer recommendation.In this paper,the contents of NH_4~+-N、NO_3~--N in soil were determined through a microplate reader with indophenol-blue spectrophotometry and barium sulfate reduction method,respectively.We selected 30 soil samples for determination and compared with that of AA3 in order to establish method for soil inorganic nitrogen determination.The results of NH_4~+-N and NO_3~--N determined by the microplate reader and the continuous flow analyzer were compared. Regression equation for NH_4~+-N was established as Y(Continuous flow analyzer-NH_4~+-N)=0.997 6 X(Microplate reader-NH_4~+-N)-0.012 3 with correlation coefficient R=0.961 9(n=30,P<0.01).Regression equation for NO_3~--N was Y(Continuous flow analyzer-NO_3~--N)=0.959 3 X(Microplate reader-NO_3~--N)+0.021 9 with correlation coefficient R=0.9640(n =30,P<0.01).The recovery rates of NH_4~+-N and NO_3~--N concentrations measured by the microplate reader were 96.2%—108% and 94.9%—110%,respectively.The relative standard deviation(RSD)of proposed colorimetric method with microplate reader was less than 10% and 5%(n=6)for NH_4~+-N and NO_3~-N,respectively.The detection limits of NH_4~+-N and NO_3~--N content by the microplate reader were 0.068 mg/L and 0.028 mg/L,respectively.It is suggested that microplate reader with colorimetric method can be used for rapid analysis of ammonium and nitrate nitrogen content in soil extracts with high accuracy and precision.
引文
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[17]温云杰,李桂花,黄金莉,等.连续流动分析仪与自动凯氏定氮仪测定小麦秸秆全氮含量之比较[J].中国土壤与肥料(Soil and Fertilizer Sciences),2015(6):146-151.
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[19]武娟,章明洪.用AA3型连续流动分析仪测定复混肥料中氨态氮的方法研究[J].化肥工业(Chemical Fertilizer Industry),2008,35(3):27-31.
[2]李永梅,杜彩琼,林春苗,等.铵态氮肥施入土壤中的转化[J].云南农业大学学报(Journal of Yunnan Agricultural University),2003,18(1):26-29.
[3]胡晓霞,丁洪,张玉树,等.菜地氮素循环及其环境效应研究进展[J].中国农学通报(Chinese Agricultural Science Bulletin),2010,26(10):287-294.
[4]LIU Y N,LI Y C,PENG Z P,et al.Effects of different nitrogen fertilizer management practices on wheat yields and N2O emissions from wheat fields in North China[J].Journal of Integrative Agriculture,2015,14(6):1184-1191.
[5]环境保护部.水质氨氮的测定蒸馏-中和滴定法:HJ 537-2009[S].北京:中国环境科学出版社,2009.
[6]环境保护部.水质氨氮的测定纳氏试剂分光光度法:HJ 535-2009[S].北京:中国环境科学出版社,2009.
[7]环境保护部.水质氨氮的测定水杨酸分光光度法:HJ 536-2009[S].北京:中国环境科学出版社,2009.
[8]苏伟波,杨张青.2种纳氏比色法检测土壤铵态氮的对比[J].中国农学通报(Chinese Agricultural Science Bulletin),2015,31(15):211-214.
[9]环境保护部.水质氨氮的测定连续流动-水杨酸分光光度法:HJ 665-2013[S].北京:中国环境科学出版社,2013.
[10]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2008.
[11]李颖.酚二磺酸分光光度法测定硝态氮影响因素研究[J].广东化工(Guangdong Chemical Industry),2015,42(7):176-178.
[12]陈岩,孟晓宏,田晓军.土壤硝态氮三种测定方法介绍与比较[J].内蒙古水利(Inner Mongolia Water Conservancy),2011(5):164-166.
[13]ROZAN T F,LUTHER G W.An anion chromatography/ultraviolet detection method to determine nitrite,nitrate,and sulfide concentrations in saline(pore)waters[J].Marine Chemistry,2002,77:1-6.
[14]冯江.酚二磺酸光度法和紫外分光光度法分析水中硝酸盐氮的比对[J].环境科学导刊(Environmental Science Guide),2007,26(B06):97-99.
[15]刘建伟,陈敏,王永霞.硫酸肼还原法测定水中硝酸盐氮[J].中国卫生工程学(Chinese Journal of Public Health Engineering),2002,1(3):175-176.
[16]卫生部.食品卫生检验方法,理化部分总则:GB/T5009.1-2003[S].北京:中国标准出版社,2004.
[17]温云杰,李桂花,黄金莉,等.连续流动分析仪与自动凯氏定氮仪测定小麦秸秆全氮含量之比较[J].中国土壤与肥料(Soil and Fertilizer Sciences),2015(6):146-151.
[18]叶祥盛,赵竹青.流动注射法与国标法测定土壤硝态氮含量的比较[J].湖北农业科学(Hubei Agricultural Sciences),2011,50(4):698-699,707.
[19]武娟,章明洪.用AA3型连续流动分析仪测定复混肥料中氨态氮的方法研究[J].化肥工业(Chemical Fertilizer Industry),2008,35(3):27-31.