辅助生殖技术实验室环境和空气质量的控制
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摘要
体外受精(IVF)实验室作为辅助生殖技术(ART)的重要组成部分,被誉为生殖中心的"心脏",对IVF的结局至关重要。辅助生殖实验室必须建立严格的质量控制与管理体系,并以提高ART活产率和安全性为根本,以规范科学为标准。实验室环境和空气质量是影响ART结局的关键因素之一,本文主要介绍了影响配子和胚胎发育的实验室环境因素及其控制方法。
In vitro fertilization(IVF) laboratory, which is known as the heart of the reproductive medical center, is an important part of assisted reproductive technology(ART) closely related to the IVF outcome. IVF laboratory must establish a strict quality control and management system based on the live birth rate and security and standard by criterion and scientific knowledge. Laboratory environment and air quality is one of the key factors contribute to successful assisted reproduction technique outcomes. This review describes several important factors and management methods of laboratory environment which would affect the development of gametes and embryos.
In vitro fertilization(IVF) laboratory, which is known as the heart of the reproductive medical center, is an important part of assisted reproductive technology(ART) closely related to the IVF outcome. IVF laboratory must establish a strict quality control and management system based on the live birth rate and security and standard by criterion and scientific knowledge. Laboratory environment and air quality is one of the key factors contribute to successful assisted reproduction technique outcomes. This review describes several important factors and management methods of laboratory environment which would affect the development of gametes and embryos.
引文
[1]Olofsson JI,Banker MR,Sjoblom LP.Quality management systems for your in vitro fertilization clinic’s laboratory:Why bother?[J].Hum Reprod Sci,2013,6(1):3-8.DOI:10.4103/0974-1208.112368.
[2]Wale PL,Gardner DK.The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction[J].Hum Reprod Update,2016,22(1):2-22.DOI:10.1093/humupd/dmv034.
[3]Boone WR,Johnson JE,Locke AJ,et al.Control of air quality in an assisted reproductive technology laboratory[J].Fertil Steril,1999,71(1):150-154.
[4]Cohen J,Gilligan A,Esposito W,et al.Ambient air and its potential effects on conception in vitro[J].Hum Reprod,1997,12(8):1742-1749.
[5]Cohen J,Gilligan A,Willadsen S.Culture and quality control of embryos[J].Hum Reprod,1998,13 Suppl 3:137-147.
[6]Hall J,Gilligan A,Schimmel T,et al.The origin,effects and control of air pollution in laboratories used for human embryo culture[J].Hum Reprod,1998,13 Suppl 4:146-155.
[7]Khoudja RY,Xu Y,Li T,et al.Better IVF outcomes following improvements in laboratory air quality[J].J Assist Reprod Genet,2013,30(1):69-76.DOI:10.1007/s10815-012-9900-1.
[8]Legro RS,Sauer MV,Mottla GL,et al.Effect of air quality on assisted human reproduction[J].Hum Reprod,2010,25(5):1317-1324.DOI:10.1093/humrep/deq021.
[9]Heitmann RJ,Hill MJ,James AN,et al.Live births achieved via IVF are increased by improvements in air quality and laboratory environment[J].Reprod Biomed Online,2015,31(3):364-371.DOI:10.1016/j.rbmo.2015.04.011.
[10]韦相才,王景园,李庆坤,等.空气质量对体外受精-胚胎移植妊娠率的影响[J].中国优生与遗传杂志,2004,12(3):124-125.
[11]毕媛,田琳,姜宙,等.不同空气质量指数期间体外受精-胚胎移植助孕结局[J].生殖医学杂志,2018,27(2):181-184.
[12]Magli MC,Van den Abbeel E,Lundin K,et al.Revised guidelines for good practice in IVF laboratories[J].Hum Reprod,2008,23(6):1253-1262.DOI:10.1093/humrep/den068.
[13]Van Voorhis BJ,Thomas M,Surrey ES,et al.What do consistently high-performing in vitro fertilization programs in the U.S.do?[J].Fertil Steril,2010,94(4):1346-1349.DOI:10.1016/j.fertnstert.2010.06.048.
[14]Mortimer D,Cohen J,Mortimer ST,et al.Cairo consensus on the IVF laboratory environment and air quality:report of an expert meeting[J].Reprod Biomed Online,2018,2,pii:S1472-6483(18)30089-0.DOI:10.1016/j.rbmo.2018.02.005.
[15]Cohen J,Alikani M,Gilligan A,et al.Setting up an ART laboratory[M]//Gardner DK,Weissman A,Howles CM,et al.Textbook of Assisted Reproductive Techniques.4t ed.Boca Raton FL:CRC Press,2012:1-8.
[16]Mortimer D.A critical assessment of the impact of the European Union Tissues and Cells Directive(2004)on laboratory practices in assisted conception[J].Reprod Biomed Online,2005,11(2):162-176.
[17]Esteves SC,Bento FC.Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive[J].Reprod Biomed Online,2013,26(1):9-21.DOI:10.1016/j.rbmo.2012.10.010.
[18]Esteves SC,Bento FC.Implementation of cleanroom technology in cleanroom technology in reproductive laboratories:the question is not why but how[J].Reprod Biomed Online,2016,32(1):9-11.DOI:10.1016/j.rbmo.2015.09.014.
[19]Hall J,Gilligan A,Schimmel T,et al.The origin,effects and control of air pollution in laboratories used for human embryo culture[J].Hum Reprod,1998,13 Suppl 4:146-155.
[20]Mehta JG,Varghese AC.Gases for embryo culture and volatile organic compounds in incubators[M]//Esteves SC,Varghese AC,Worrilow KC.Clean Room Technology in ART Clinics:A Practical Guide.Boca Raton FL:CRC Press,2016:99-118.
[21]Swain JE.Decisions for the IVF laboratory:comparative analysis of embryo culture incubators[J].Reprod Biomed Online,2014,28(5):535-547.DOI:10.1016/j.rbmo.2014.01.004.
[22]Merton JS,Vermeulen ZL,Otter T,et al.Carbon-activated gas filtration during in vitro culture increased pregnancy rate following transfer of in vitro-produced bovine embryos[J].Theriogenology,2007,1567(7):1233-1238.
[23]Swain JE,Cabrera L,Xu X,et al.Microdrop preparation factors influence culture-media osmolality,which can impair mouse embryo preimplantation development[J].Reprod Biomed Online,2012,24(2):142-147.DOI:10.1016/j.rbmo.2011.10.008.
[24]徐维海,王慧红,周文静,等.胚胎实验室挥发性有机化合物的处理[J].浙江预防医学,2014,26(5):538-540.
[2]Wale PL,Gardner DK.The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction[J].Hum Reprod Update,2016,22(1):2-22.DOI:10.1093/humupd/dmv034.
[3]Boone WR,Johnson JE,Locke AJ,et al.Control of air quality in an assisted reproductive technology laboratory[J].Fertil Steril,1999,71(1):150-154.
[4]Cohen J,Gilligan A,Esposito W,et al.Ambient air and its potential effects on conception in vitro[J].Hum Reprod,1997,12(8):1742-1749.
[5]Cohen J,Gilligan A,Willadsen S.Culture and quality control of embryos[J].Hum Reprod,1998,13 Suppl 3:137-147.
[6]Hall J,Gilligan A,Schimmel T,et al.The origin,effects and control of air pollution in laboratories used for human embryo culture[J].Hum Reprod,1998,13 Suppl 4:146-155.
[7]Khoudja RY,Xu Y,Li T,et al.Better IVF outcomes following improvements in laboratory air quality[J].J Assist Reprod Genet,2013,30(1):69-76.DOI:10.1007/s10815-012-9900-1.
[8]Legro RS,Sauer MV,Mottla GL,et al.Effect of air quality on assisted human reproduction[J].Hum Reprod,2010,25(5):1317-1324.DOI:10.1093/humrep/deq021.
[9]Heitmann RJ,Hill MJ,James AN,et al.Live births achieved via IVF are increased by improvements in air quality and laboratory environment[J].Reprod Biomed Online,2015,31(3):364-371.DOI:10.1016/j.rbmo.2015.04.011.
[10]韦相才,王景园,李庆坤,等.空气质量对体外受精-胚胎移植妊娠率的影响[J].中国优生与遗传杂志,2004,12(3):124-125.
[11]毕媛,田琳,姜宙,等.不同空气质量指数期间体外受精-胚胎移植助孕结局[J].生殖医学杂志,2018,27(2):181-184.
[12]Magli MC,Van den Abbeel E,Lundin K,et al.Revised guidelines for good practice in IVF laboratories[J].Hum Reprod,2008,23(6):1253-1262.DOI:10.1093/humrep/den068.
[13]Van Voorhis BJ,Thomas M,Surrey ES,et al.What do consistently high-performing in vitro fertilization programs in the U.S.do?[J].Fertil Steril,2010,94(4):1346-1349.DOI:10.1016/j.fertnstert.2010.06.048.
[14]Mortimer D,Cohen J,Mortimer ST,et al.Cairo consensus on the IVF laboratory environment and air quality:report of an expert meeting[J].Reprod Biomed Online,2018,2,pii:S1472-6483(18)30089-0.DOI:10.1016/j.rbmo.2018.02.005.
[15]Cohen J,Alikani M,Gilligan A,et al.Setting up an ART laboratory[M]//Gardner DK,Weissman A,Howles CM,et al.Textbook of Assisted Reproductive Techniques.4t ed.Boca Raton FL:CRC Press,2012:1-8.
[16]Mortimer D.A critical assessment of the impact of the European Union Tissues and Cells Directive(2004)on laboratory practices in assisted conception[J].Reprod Biomed Online,2005,11(2):162-176.
[17]Esteves SC,Bento FC.Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive[J].Reprod Biomed Online,2013,26(1):9-21.DOI:10.1016/j.rbmo.2012.10.010.
[18]Esteves SC,Bento FC.Implementation of cleanroom technology in cleanroom technology in reproductive laboratories:the question is not why but how[J].Reprod Biomed Online,2016,32(1):9-11.DOI:10.1016/j.rbmo.2015.09.014.
[19]Hall J,Gilligan A,Schimmel T,et al.The origin,effects and control of air pollution in laboratories used for human embryo culture[J].Hum Reprod,1998,13 Suppl 4:146-155.
[20]Mehta JG,Varghese AC.Gases for embryo culture and volatile organic compounds in incubators[M]//Esteves SC,Varghese AC,Worrilow KC.Clean Room Technology in ART Clinics:A Practical Guide.Boca Raton FL:CRC Press,2016:99-118.
[21]Swain JE.Decisions for the IVF laboratory:comparative analysis of embryo culture incubators[J].Reprod Biomed Online,2014,28(5):535-547.DOI:10.1016/j.rbmo.2014.01.004.
[22]Merton JS,Vermeulen ZL,Otter T,et al.Carbon-activated gas filtration during in vitro culture increased pregnancy rate following transfer of in vitro-produced bovine embryos[J].Theriogenology,2007,1567(7):1233-1238.
[23]Swain JE,Cabrera L,Xu X,et al.Microdrop preparation factors influence culture-media osmolality,which can impair mouse embryo preimplantation development[J].Reprod Biomed Online,2012,24(2):142-147.DOI:10.1016/j.rbmo.2011.10.008.
[24]徐维海,王慧红,周文静,等.胚胎实验室挥发性有机化合物的处理[J].浙江预防医学,2014,26(5):538-540.