长链非编码RNA LINC00152在喉鳞状细胞癌中的表达及其临床意义
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摘要
目的:探讨长链非编码RNA LINC00152在喉鳞状细胞癌(LSCC)中的表达及其与临床病理特征的相关性。方法:通过实时荧光定量PCR的方法,检测LINC00152在LSCC细胞系以及36对LSCC癌组织与配对癌旁正常组织中的相对表达水平,并分析其表达水平与患者年龄、吸烟、饮酒、病理分化程度、临床分期及颈部淋巴结转移的相关性。结果:LINC00152在LSCC细胞系中呈高表达,且在癌组织中的相对表达水平明显高于配对癌旁正常组织(P=0.006),其表达水平与患者病理分化程度(P=0.032)、临床分期(P=0.044)相关,与患者年龄、吸烟、饮酒及颈部淋巴结转移无明显相关性(P>0.05)。结论:LINC00152在LSCC中表达增高,其有可能作为LSCC临床诊断、治疗和预后的标志物。
Objective:The aim of this study is to explore the relative expression level of LINC00152 in laryngeal squamous cell carcinoma(LSCC)and its clinical significance.Method:The relative expression levels of LINC00152 in LSCC cell lines and 36 paired LSCC specimens were measured by qRT-PCR method.And the correlations between the expression level of LINC00152 and the clinical features derived from LSCC patients were analyzed and compared through the independent sample t-test.Result:The relative expression level of LINC00152 was over-expressed in LSCC cell lines and cancerous tissues than that in paired adjacent normal tissues,and the difference was statistically significant(P=0.006).Even the associations between LINC00152 expression level and clinicopathological features(P=0.044 for clinical stage,P=0.032 for pathological differentiation degree)were significantly.Conclusion:LINC00152 is highly expressed in LSCC and it may become a new tumor marker for the diagnosis and prognosis of LSCC.
Objective:The aim of this study is to explore the relative expression level of LINC00152 in laryngeal squamous cell carcinoma(LSCC)and its clinical significance.Method:The relative expression levels of LINC00152 in LSCC cell lines and 36 paired LSCC specimens were measured by qRT-PCR method.And the correlations between the expression level of LINC00152 and the clinical features derived from LSCC patients were analyzed and compared through the independent sample t-test.Result:The relative expression level of LINC00152 was over-expressed in LSCC cell lines and cancerous tissues than that in paired adjacent normal tissues,and the difference was statistically significant(P=0.006).Even the associations between LINC00152 expression level and clinicopathological features(P=0.044 for clinical stage,P=0.032 for pathological differentiation degree)were significantly.Conclusion:LINC00152 is highly expressed in LSCC and it may become a new tumor marker for the diagnosis and prognosis of LSCC.
引文
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[3]LI M,NING J,LI Z,et al.LINC00152promotes the growth and invasion of oral squamous cell carcinoma by regulating miR-139-5p[J].Onco Targets Ther,2018,11:6295-6304.
[4]SUN Z,GUO X,ZANG M,et al.Long non-coding RNA LINC00152promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR-497/BDNF axis[J].J Cell Physiol,2019,234:1336-1345.
[5]REON B J,TAKAO REAL KARIA B,KIRAN M,et al.LINC00152Promotes Invasion through a 3'-Hairpin Structure and Associates with Prognosis in Glioblastom[J].Mol Cancer Res,2018,16:1470-1482.
[6]CANCER GENOME ATLAS RESEARCH NET-WORK.Comprehensive genomic characterization defines human glioblastoma genes and core pathways[J].Nature,2008,455:1061-1068.
[7]葛珊珊,吴勇延,高伟,等.长链非编码RNALINC00460在喉鳞状细胞癌中的表达及意义[J].临床耳鼻咽喉头颈外科杂志,2018,32(1):18-22.
[8]SHI X,SUN M,LIU H,et al.Long non-coding RNAs:a new frontier in the study of human diseases[J].Cancer Lett,2013,339:159-166.
[9]ZHAO L,CAO H,CHI W,et al.Expression profile analysis identifies the long non-coding RNA landscape and the potential carcinogenic functions of LINC00668in laryngeal squamous cell carcinoma[J].Gene,2019,687:47-55.
[10]YU J,LIU Y,GUO C,et al.Upregulated long noncoding RNA LINC00152expression is associated with progression and poor prognosis of tongue squamous cell carcinoma[J].J Cancer,2017,8:523-530.
[11]HU X L,WANG J,HE W,et al.Down-regulation of lncRNA Linc00152suppressed cell viability,invasion,migration,and epithelial to mesenchymal transition,and reversed chemo-resistance in breast cancer cells[J].Eur Rev Med Pharmacol Sci,2018,22:3074-3084.
[12]ZHANG J,YIN M,HUANG J,et al.Long noncoding RNA LINC00152as a novel predictor of lymph node metastasis and survival in human cancer:a systematic review and meta-analysis[J].Clin Chim Acta,2018,483:25-32.
[13]MIAO C,ZHAO K,ZHU J,et al.Clinicopathological and Prognostic Role of Long Noncoding RNALinc00152in Various Human Neoplasms:Evidence from Meta-Analysis[J].Biomed Res Int,2017,2017:6010721.