壮药牛大力质量标准的提高研究
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摘要
目的:提高壮药牛大力的质量标准。方法:对牛大力药材的显微鉴别、薄层鉴别、水分、总灰分、酸不溶性灰分、浸出物和含量测定等进行研究。结果:确定了牛大力根横切面和粉末显微特征;以高丽槐素为对照的薄层鉴别分离度好、重现性佳;10批牛大力样品水分8.0%～13.0%,总灰分1.9%～4.4%,酸不溶性灰分0.1%～1.0%,水溶性浸出物(热浸法)12.2%～32.9%,50%乙醇浸出物(热浸法)8.2%～19.4%;高丽槐素进样量在0.098～1.960μg范围内与峰面积呈现良好的线性关系(r=0.9999,n=6),平均回收率为99.06%,RSD%为1.59%(n=6)。结论:本研究所建立的方法操作简单、合理可行,可为提高和完善牛大力药材质量标准提供实验依据。
Objective: To improve the quality standard of Zhuang medicine Niudali(Millettia Speciosa Root).Methods: The tests of microscopic and TLC identification, the water and total ash and acid-insoluble ash inspections, extractum and content determination were carried out. Results: Microscopic characteristics of root cross-section and powder were confirmed; The TLC identification of the Maackiain had good degree of separation and reproducibility; The moisture contents were 8.0%-13.0%; total ash contents were 1.9%-4.4%;acid-insoluble ash contents were 0.1%-1.0%; water-soluble extract(hot dip method) contents were 12.2%-32.9%,50%; ethanol extract(hot dip method) contents were 8.2%-19.4%. The HPLC of the Maackiain of Beautiful millettia root was set up and Maackiain in the range of 0.098 μg-1.960 μg showed good linearrelationship(r=0.9999, n=6); the average recovery was 99.06%, and RSD was 1.59%(n=6). Conclusion: The experimental data and the established method can provide the experimental basis for the improvement of the quality standard of Niudali(Millettia Speciosa Root).
Objective: To improve the quality standard of Zhuang medicine Niudali(Millettia Speciosa Root).Methods: The tests of microscopic and TLC identification, the water and total ash and acid-insoluble ash inspections, extractum and content determination were carried out. Results: Microscopic characteristics of root cross-section and powder were confirmed; The TLC identification of the Maackiain had good degree of separation and reproducibility; The moisture contents were 8.0%-13.0%; total ash contents were 1.9%-4.4%;acid-insoluble ash contents were 0.1%-1.0%; water-soluble extract(hot dip method) contents were 12.2%-32.9%,50%; ethanol extract(hot dip method) contents were 8.2%-19.4%. The HPLC of the Maackiain of Beautiful millettia root was set up and Maackiain in the range of 0.098 μg-1.960 μg showed good linearrelationship(r=0.9999, n=6); the average recovery was 99.06%, and RSD was 1.59%(n=6). Conclusion: The experimental data and the established method can provide the experimental basis for the improvement of the quality standard of Niudali(Millettia Speciosa Root).
引文
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[3]黎瑞汝,陈占科,高珊,等.中药牛大力的研究进展[J].亚太传统医药,2010,6(12):165-167.
[4]韦玉燕,巫繁菁,曾海生,等.牛大力研究概况[J].广西科学院学报,2010,26(3):380-382.
[5]吴世芳,查和萍,范志勇,等.查氏膝痹方治疗膝关节骨性关节炎浅述[J].中医药导报,2014,20(3):5-8.
[6]陈旦妃,陈高峰,张念华,等.自拟固本安神汤治疗肺癌伴失眠的疗效观察[J].中医药导报,2017,23(11):65-67.
[7]张美艺,刘孟渊.刘孟渊教授治疗膝骨关节炎临床经验总结[J].中医药导报,2018,24(10):130-131.
[8]牛秀丽,张天鹏,石红,等.壮腰健肾小蜜丸的质量标准研究[J].河北中医药学报,2015,30(2):58-60.
[9]钟霞军,涂冬萍,王柳萍.牛大力的化学成分及药理作用研究进展[J].广西中医药大学学报,2015,18(3):73-75.
[10]陈黄保.甜牛大力和苦牛大力的生药研究[J].中草药,2001,32(9):843-845.
[11]禹建春,李美琴,徐冠,等.牛大力药材的鉴别研究[J].山东中医杂志,2012,31(12):897-898.
[12]吴鲁东,张戴英,余馥佳,等.牛大力的质量评价研究[J].今日药学,2015,25(2):90-92.
[13]刘若轩,欧倩雯,吕竹芬,等.牛大力饮片的质量标准研究[J].今日药学,2015,25(1):18-20.
[14]陈晨,刘积光,赵震宇,等.海南牛大力质量控制标准研究[J].食品工业,2016,37(8):289-293.
[15]王春华,王勇,王英,等.RP-HPLC法测定牛大力药材中高丽槐素的含量[J].药物分析杂志,2010,30(9):1735-1737.
[16]李锐,陈国彪.HPLC法测定牛大力中高丽槐素的含量[J].中国药事,2011,25(2):124-125,140.
[17]陈勇,谢臻,巫繁菁,等.HPLC测定广西牛大力药材中芒柄花素和高丽槐素的含量[J].世界科学技术-中医药现代化,2013,15(2):260-263.
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