正交实验优选萹蓄中绿原酸的提取工艺
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摘要
[目的]对萹蓄绿原酸的提取方法进行优选,确定最佳提取工艺。[方法]采用正交实验表L_8(2~7)安排实验。[结果]提取溶媒对萹蓄绿原酸百分含量影响最大。[结论]确定萹蓄中绿原酸的最佳提取工艺是A_2B_2C_2D_2,即加50%甲醇超声提取,提取次数为2次,每次1 h。
[Objective]To determine and select the best extaction process for chlorogenic acid in Polygonum aviculare L. the best process was selected. [Methods]Using the L_8(2~7) orthogonal table. [Results]The extracting solution is the significant factor affecting the extraction process. [Conclusion]The best extracting process is A_2B_2C_2D_2, that is adding 50% Methyl alcohol supersonic extraction 2 times, and 1 h for each time.
[Objective]To determine and select the best extaction process for chlorogenic acid in Polygonum aviculare L. the best process was selected. [Methods]Using the L_8(2~7) orthogonal table. [Results]The extracting solution is the significant factor affecting the extraction process. [Conclusion]The best extracting process is A_2B_2C_2D_2, that is adding 50% Methyl alcohol supersonic extraction 2 times, and 1 h for each time.
引文
[1] 邓仕任,朱夏敏,王鑫,等.高效液相色谱法测定萹蓄中绿原酸含量[J].广州化工,2016,44(11):152-153.
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[3] 于兆海,孙结.萹蓄的化学成分研究[J].中草药,2002(6):498-499.
[4] 荣志芬,张慰青.复方止咳冲剂中甘草酸和绿原酸的高效液相色谱法测定[J].中草药,1995(4):181-182.
[5] 刘传安,邹盛勤.RP-HPLC法测定陆英叶中绿原酸含量的研究[J].安徽农业科学,2005,33(12):2334-2335.
[6] 张艳焱,张天伦,梁光义,等.黄褐毛忍冬不同炮制品中绿原酸的HPLC法含量测定[J].中草药,2003,34(8):696-697.
[2] 张蓉,魏希颖.扁蓄体外抑菌作用及其绿原酸含量测定[J].陕西农业科学,2010,56(6):64-66.
[3] 于兆海,孙结.萹蓄的化学成分研究[J].中草药,2002(6):498-499.
[4] 荣志芬,张慰青.复方止咳冲剂中甘草酸和绿原酸的高效液相色谱法测定[J].中草药,1995(4):181-182.
[5] 刘传安,邹盛勤.RP-HPLC法测定陆英叶中绿原酸含量的研究[J].安徽农业科学,2005,33(12):2334-2335.
[6] 张艳焱,张天伦,梁光义,等.黄褐毛忍冬不同炮制品中绿原酸的HPLC法含量测定[J].中草药,2003,34(8):696-697.