核桃内果皮木质素生物合成途径关键基因研究进展
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摘要
木质素(Lignin)是陆生维管植物次生细胞壁中含量丰富的高分子有机化合物,与植物细胞壁内的纤维素和半纤维素交织在一起。木质素在核桃硬壳(内果皮)中含量丰富且是其构成的主要成分,含量约在60%以上,目前生产中核桃缝合线松弛以及核桃内果皮发育不全(露仁)现象成为制约新疆核桃优质高产的主要因素,新疆要实现核桃的优质高产以及木质素的充分合理的利用,需进一步了解木质素的结构、组成及关键基因表达调控。因此,重点对木质素结构、木质素生物合成过程及基因调控规律等方面分析基因调控规律,为从植物木质素合成及表达量方面改善新疆核桃品质奠定理论基础。
引文
[1]徐凯,夏玉芳,李允祥,等.贞丰核桃主要经济性状评价[J].种子,2016,35(04):118-121.
[2]田贝贝,高疆生,赵明,等.南疆核桃品种脂肪酸及氨基酸的营养评价[J].新疆农业科学,2009,46(05):970-974.
[3]李夕勃,赵书岗,土红霞,等.核桃坚果硬壳结构与木质素和纤维素相关性研究[J].湖北农业科学,2012,51(22):5076-5079.
[4]郭光艳,柏峰,刘伟,等.转录因子对木质素生物合成调控的研究进展[J/OL].中国农业科学,2015,48(07):1277-1287.(2015-04-03).
[5]赵书岗,赵悦平,王红霞,等.核桃坚果硬壳结构的影响因子[J].林业科学,2011,47(4):70-75.
[6]张永涛,张袅明,秦博妮,等.用于废水中重金属吸附的核桃壳吸附剂制备方法研究进展[J].延安大学学报:自然科学版,2014(4):69-73.
[7]Ghazi Mokri H S,Modirshahla N,Behnajady M A,et al.Adsorption of C.I.Acid R ed 97 dye from aqueous solution onto walnut shell:inetics,thermodynamics parameters,isotherms[J].Int J Environ Sci Tecchnol,2015(12):1401-1408.
[8]郑志锋,邹局春,陈浪,等.核桃壳木质素的1H-NMR分析[J].西北林学院学报,2007,22(2):131-133.
[9]李夕勃,赵书岗,土红霞,等.核桃坚果硬壳结构与木质素和纤维素相关性研究[J].湖北农业科学,2012,51(22):5076-5079.
[10]Kazmi M,Feroze N,Javed H,et al.Biosorption of cropper(II)on dry fruit by product:characterization,kinetic and equilibrium studies[J].Journal of the Chemical Society of Pakistan,2012,34(6):1356-1365.
[11]HAhmad Nauman Shah Saqib,Amir Waseem,Ather Farooq Khan,et al.Arsenic bioremediation by low cost materials derived from blue pine(Pinus wallichiana)and walnut Juglans regia[J].Ecological Engineering,2013,5:88-94.
[12]李荣华,张院民,张增强,等.农业废弃物核桃壳粉对Cr(VI)的吸附特征研究[J].农业环境科学学报,2009,28(8):1693-1700.
[13]许振,李云春,姜友军,等.核桃壳粉对水溶液中Pb-+的吸附[J].环境工程学报,2012,6(12):4504-4512.
[14]朱晨杰,张会岩,肖睿,等.木质纤维素高值化利用的研究进展[J].中国科学:化学,2015,45(05):454-478.
[15]葛少彬,刘敏,蔡昆争,等.硅介导稻瘟病抗性的生理机理[J/O L].中国农业科学,2014,47(02):240-251.
[16]罗华超,任世学,马艳丽,等.聚乙烯醇-碱木质素发泡材料的制备与性能[J].北京林业大学学报,2015,37(04):127-134.
[17]路瑶,魏贤勇,宗志敏,等.木质素的结构研究与应用[J].化学进展,2013,25(05):838-858.
[18]胡可,严雪锋,栗丹,等.沉默CCR和CAD基因培育低木质素含量转基因多年生黑麦草[J].草业学报,2013,22(05):72-83.
[19]Jorge R,Ana G,Lidia N,et al.Lignin Composition and Structure in Young versus Adult Eucalyptus globulus[J].Plants,2011,155:667-682.
[20]王斌,张楠,闫冲冲,等.套袋对砀山酥梨果实石细胞发育及木质素代谢的影响[J].园艺学报,2013,40(03):531-539.
[21]宋修鹏,黄杏,莫凤连,等.甘蔗苯丙氨酸解氨酶基因(PAL)的克隆和表达分析[J].中国农业科学,2013,46(14):2856-2868.
[22]Korth,K L,Blount J W,Chen F,R asmussen,et al.Changes in Phenylpropanoid Metabolites Associated with Homology-dependent Silencing of Phenylalanine Ammonialyase and its Somatic R eversion in Tobacco[J].Physiol Plant 2001,111:137-143.
[23]邹丽红,张玉星.砂梨果肉褐变与酚类物质及相关酶活性的相关分析[J].果树学报,2012(6):1022-1026.
[24]Sewalt V J H,Ni W,Blount J W,et al.R educed lignin content and altered lignin composition in transgenic tobacco down-reg ulated in expression of L-pheny lalanine ammonia-yase or cinnamate 4-hy droxylase[J].Plant Physiol,1997,115(1):41-50.
[25]Blee K,Choi J W,Conneu.A P,et al.Antisense and sense expression of c DNA coding for CYP73A15,a classsⅡcinnamate 4-hydroxylase,leads to a delayed and reduced production of lignin in tobacco[J].Phytochemistry,2001,57(7):1159-1166.
[26]田晓明,颜立红,向光锋,等.植物4-香豆酸:辅酶A连接酶研究进展[J].生物技术通报,2017,33(04):19-26.
[27]曹运鹏,方志,李姝妹,等,砀山酥梨4CL基因家族的全基因组鉴定与分析[J].遗传2015,37(07):711-719.
[28]倪志勇,王娟,吕萌,等.棉花4-香豆酸辅酶A连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.
[29]黄满芬,王恒,方荣俊,等.桑树肉桂酸-4-轻化酶基因(MmC4H)的克隆及在不同桑品种间的表达差异[J].蚕业科学,2014,40(4):592-600.
[30]Tsuji Y,Chen F,Yasuda S,et al.Unexpected behavior of coniferin in lignin biosynthesis of Ginkgo biloba L.[J].Planta,2005,222:58-69.
[31]R eddy M S,Chen F,Shadle G,et al.Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa(Medicago sativa L.)[J].Proc Natl Acad Sci USA,2005,102(46):16573-16578.
[32]Coleman H D,Park J Y,Nair R,et al.R NAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism[J].Proc Natl Acad Sci USA105,2008,4501-4506.
[33]R alph J,Akiyama T,Coleman H D,et al.Effects on lignin structure of coumarate 3-hydroxylase downregulation in poplar[J].BioEnergy R esearch,2015,5(4):1009-1019.
[34]Vignols F,R igau J,Torres M.A.The brown midrib3(bm3)mutation in maize occurs in the gene encoding caffeic acid O-methyl-transferase[J].Plant Cell,1995,7(4):407-416.
[35]R astogi S,Dwivedi U N.Down-R egulation of Lignin Biosynthesis in Transgenic Leucaena leucocephala Harboring O-Methyltransferase Gene[J].Biotechnol Prog,2006,22(3):609-616.
[36]Dien B S,Miller D J,Hector R E,et al.Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition[J].Bioresource Technology,2011,102(11):6479-6486.
[37]Chen L,Auh C,bowling P,et al.Transgenic down-regulation of caffeic acid O-methyltransferase(CO MT)led to improved digestibility in tall fescue(Festuca arundlinacea)functional[J].Plant Biology,2004,31(3):1235-245.
[38]李波,倪志勇,王娟,等.木质素生物合成关键酶咖啡酸-O-甲基转移酶基因(COMT)的研究进展[J].分子植物育种,2010,1(8):117-124.
[39]Ye Z H,Zhong R,Morrison Iii W H,Himmelsbach D.S.Caffeoyl coenzyme A-O-methyltransferase and lignin iosynthesis[J].Phytochemistry,2001,57(7):1177-1185.
[40]Do C T,Pollet B,Thevenin J,et al.Both caffeoyl Coenzyme A3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin,flavonoids and sinapoyl malate biosynthesis in Arabidopsis[J].Planta,2007,226:1117-1129.
[41]Guo D,Chen F,INoue K,et al.Downregulation of caffeic acid3-O-methyltransferase and caffeoyl CoA 3-O-methyl-transferase in transgenic alfalfa:impacts on lignin structure and implications for the biosynthesis of G and S lignin[J].Plant Cell,2001,13(1):73-88.
[42]胡丹,刘星贝,汪灿,等.不同抗倒性甜荞茎秆木质素合成关键酶基因的表达分析[J].中国农业科学,2015,48(09):1864-1872.
[43]谷振军,章怀云,张党权,等.赤桉CCoAO MT亚家族的基因克隆及可变剪接分析[J].林业科学,2014,50(05):62-69.
[44]Day A,Neutelings G,Nolin F,et al.Caffeoyl coenzyme A-o-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem[J],Plant Physiol.Bioch.,2009,47(1):9-19.
[45]魏建华,王彦珍,王宏芝,等.抑制CCoAO MT表达的转基因杨树的制浆性能[J].科学通报,2008,53(21):2612-2616.
[46]R alph J,Hatfield R.D,Piquemal J,et al.NMR characterization of altered lignins extracted from tobacco plants down-regu-lated for lignification enzymies cinnamyl-alcohol dehydroge-nase and cinnamoyl-CoAreductase[J].Proc.Natl.Acad.Sci.,1998,95(22):12803-12808.
[47]Leole J C,Dauwe R,Morreel K,et al.Downregulation of cinnamoyl-coenzyme A reductase in poplar:multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure[J].Plant Cell.2007,19(11):3669-3691.
[48]Tamasloukht B,Wong Q uai Lam M S,Martinez Y,et al.Characterization of a cinnamoyl-CoA reductase 1(CCR 1)mutant in maize:effects on lignification,fibre development,and global gene expression[J].Joumal of Experimental Botany,2011,62(11):3837-3848.
[49]胡可,严雪锋,栗丹,等.沉默CCR和CAD基因培育低木质素含量转基因多年生黑麦草[J].草业学报,2013,22(05):72-83.
[50]Thevenin J,Pollet B,Letarnec B.The simultaneous repression of CCR and CAD,two enzymes of the lignin biosynthetic pathway,results in sterility and dwarfism in Arabidopsis thaliana[J].Molecular Plant,2011,4(1):70-82.
[51]孔华,郭安平,郭运玲,等.木质素生物合成及转基因调控研究进展[J].热带农业工程,2009,33(5):47-53.
[52]Martínez-García P J.Crepeau M W,Puiu D,et al.The walnut(Juglans regia)genome sequence reveals diversity in genes coding for the biosynthesis of non-structural polyphenols[J].Plant J,2016,87(5):507 532.
[2]田贝贝,高疆生,赵明,等.南疆核桃品种脂肪酸及氨基酸的营养评价[J].新疆农业科学,2009,46(05):970-974.
[3]李夕勃,赵书岗,土红霞,等.核桃坚果硬壳结构与木质素和纤维素相关性研究[J].湖北农业科学,2012,51(22):5076-5079.
[4]郭光艳,柏峰,刘伟,等.转录因子对木质素生物合成调控的研究进展[J/OL].中国农业科学,2015,48(07):1277-1287.(2015-04-03).
[5]赵书岗,赵悦平,王红霞,等.核桃坚果硬壳结构的影响因子[J].林业科学,2011,47(4):70-75.
[6]张永涛,张袅明,秦博妮,等.用于废水中重金属吸附的核桃壳吸附剂制备方法研究进展[J].延安大学学报:自然科学版,2014(4):69-73.
[7]Ghazi Mokri H S,Modirshahla N,Behnajady M A,et al.Adsorption of C.I.Acid R ed 97 dye from aqueous solution onto walnut shell:inetics,thermodynamics parameters,isotherms[J].Int J Environ Sci Tecchnol,2015(12):1401-1408.
[8]郑志锋,邹局春,陈浪,等.核桃壳木质素的1H-NMR分析[J].西北林学院学报,2007,22(2):131-133.
[9]李夕勃,赵书岗,土红霞,等.核桃坚果硬壳结构与木质素和纤维素相关性研究[J].湖北农业科学,2012,51(22):5076-5079.
[10]Kazmi M,Feroze N,Javed H,et al.Biosorption of cropper(II)on dry fruit by product:characterization,kinetic and equilibrium studies[J].Journal of the Chemical Society of Pakistan,2012,34(6):1356-1365.
[11]HAhmad Nauman Shah Saqib,Amir Waseem,Ather Farooq Khan,et al.Arsenic bioremediation by low cost materials derived from blue pine(Pinus wallichiana)and walnut Juglans regia[J].Ecological Engineering,2013,5:88-94.
[12]李荣华,张院民,张增强,等.农业废弃物核桃壳粉对Cr(VI)的吸附特征研究[J].农业环境科学学报,2009,28(8):1693-1700.
[13]许振,李云春,姜友军,等.核桃壳粉对水溶液中Pb-+的吸附[J].环境工程学报,2012,6(12):4504-4512.
[14]朱晨杰,张会岩,肖睿,等.木质纤维素高值化利用的研究进展[J].中国科学:化学,2015,45(05):454-478.
[15]葛少彬,刘敏,蔡昆争,等.硅介导稻瘟病抗性的生理机理[J/O L].中国农业科学,2014,47(02):240-251.
[16]罗华超,任世学,马艳丽,等.聚乙烯醇-碱木质素发泡材料的制备与性能[J].北京林业大学学报,2015,37(04):127-134.
[17]路瑶,魏贤勇,宗志敏,等.木质素的结构研究与应用[J].化学进展,2013,25(05):838-858.
[18]胡可,严雪锋,栗丹,等.沉默CCR和CAD基因培育低木质素含量转基因多年生黑麦草[J].草业学报,2013,22(05):72-83.
[19]Jorge R,Ana G,Lidia N,et al.Lignin Composition and Structure in Young versus Adult Eucalyptus globulus[J].Plants,2011,155:667-682.
[20]王斌,张楠,闫冲冲,等.套袋对砀山酥梨果实石细胞发育及木质素代谢的影响[J].园艺学报,2013,40(03):531-539.
[21]宋修鹏,黄杏,莫凤连,等.甘蔗苯丙氨酸解氨酶基因(PAL)的克隆和表达分析[J].中国农业科学,2013,46(14):2856-2868.
[22]Korth,K L,Blount J W,Chen F,R asmussen,et al.Changes in Phenylpropanoid Metabolites Associated with Homology-dependent Silencing of Phenylalanine Ammonialyase and its Somatic R eversion in Tobacco[J].Physiol Plant 2001,111:137-143.
[23]邹丽红,张玉星.砂梨果肉褐变与酚类物质及相关酶活性的相关分析[J].果树学报,2012(6):1022-1026.
[24]Sewalt V J H,Ni W,Blount J W,et al.R educed lignin content and altered lignin composition in transgenic tobacco down-reg ulated in expression of L-pheny lalanine ammonia-yase or cinnamate 4-hy droxylase[J].Plant Physiol,1997,115(1):41-50.
[25]Blee K,Choi J W,Conneu.A P,et al.Antisense and sense expression of c DNA coding for CYP73A15,a classsⅡcinnamate 4-hydroxylase,leads to a delayed and reduced production of lignin in tobacco[J].Phytochemistry,2001,57(7):1159-1166.
[26]田晓明,颜立红,向光锋,等.植物4-香豆酸:辅酶A连接酶研究进展[J].生物技术通报,2017,33(04):19-26.
[27]曹运鹏,方志,李姝妹,等,砀山酥梨4CL基因家族的全基因组鉴定与分析[J].遗传2015,37(07):711-719.
[28]倪志勇,王娟,吕萌,等.棉花4-香豆酸辅酶A连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.
[29]黄满芬,王恒,方荣俊,等.桑树肉桂酸-4-轻化酶基因(MmC4H)的克隆及在不同桑品种间的表达差异[J].蚕业科学,2014,40(4):592-600.
[30]Tsuji Y,Chen F,Yasuda S,et al.Unexpected behavior of coniferin in lignin biosynthesis of Ginkgo biloba L.[J].Planta,2005,222:58-69.
[31]R eddy M S,Chen F,Shadle G,et al.Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa(Medicago sativa L.)[J].Proc Natl Acad Sci USA,2005,102(46):16573-16578.
[32]Coleman H D,Park J Y,Nair R,et al.R NAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism[J].Proc Natl Acad Sci USA105,2008,4501-4506.
[33]R alph J,Akiyama T,Coleman H D,et al.Effects on lignin structure of coumarate 3-hydroxylase downregulation in poplar[J].BioEnergy R esearch,2015,5(4):1009-1019.
[34]Vignols F,R igau J,Torres M.A.The brown midrib3(bm3)mutation in maize occurs in the gene encoding caffeic acid O-methyl-transferase[J].Plant Cell,1995,7(4):407-416.
[35]R astogi S,Dwivedi U N.Down-R egulation of Lignin Biosynthesis in Transgenic Leucaena leucocephala Harboring O-Methyltransferase Gene[J].Biotechnol Prog,2006,22(3):609-616.
[36]Dien B S,Miller D J,Hector R E,et al.Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition[J].Bioresource Technology,2011,102(11):6479-6486.
[37]Chen L,Auh C,bowling P,et al.Transgenic down-regulation of caffeic acid O-methyltransferase(CO MT)led to improved digestibility in tall fescue(Festuca arundlinacea)functional[J].Plant Biology,2004,31(3):1235-245.
[38]李波,倪志勇,王娟,等.木质素生物合成关键酶咖啡酸-O-甲基转移酶基因(COMT)的研究进展[J].分子植物育种,2010,1(8):117-124.
[39]Ye Z H,Zhong R,Morrison Iii W H,Himmelsbach D.S.Caffeoyl coenzyme A-O-methyltransferase and lignin iosynthesis[J].Phytochemistry,2001,57(7):1177-1185.
[40]Do C T,Pollet B,Thevenin J,et al.Both caffeoyl Coenzyme A3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin,flavonoids and sinapoyl malate biosynthesis in Arabidopsis[J].Planta,2007,226:1117-1129.
[41]Guo D,Chen F,INoue K,et al.Downregulation of caffeic acid3-O-methyltransferase and caffeoyl CoA 3-O-methyl-transferase in transgenic alfalfa:impacts on lignin structure and implications for the biosynthesis of G and S lignin[J].Plant Cell,2001,13(1):73-88.
[42]胡丹,刘星贝,汪灿,等.不同抗倒性甜荞茎秆木质素合成关键酶基因的表达分析[J].中国农业科学,2015,48(09):1864-1872.
[43]谷振军,章怀云,张党权,等.赤桉CCoAO MT亚家族的基因克隆及可变剪接分析[J].林业科学,2014,50(05):62-69.
[44]Day A,Neutelings G,Nolin F,et al.Caffeoyl coenzyme A-o-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem[J],Plant Physiol.Bioch.,2009,47(1):9-19.
[45]魏建华,王彦珍,王宏芝,等.抑制CCoAO MT表达的转基因杨树的制浆性能[J].科学通报,2008,53(21):2612-2616.
[46]R alph J,Hatfield R.D,Piquemal J,et al.NMR characterization of altered lignins extracted from tobacco plants down-regu-lated for lignification enzymies cinnamyl-alcohol dehydroge-nase and cinnamoyl-CoAreductase[J].Proc.Natl.Acad.Sci.,1998,95(22):12803-12808.
[47]Leole J C,Dauwe R,Morreel K,et al.Downregulation of cinnamoyl-coenzyme A reductase in poplar:multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure[J].Plant Cell.2007,19(11):3669-3691.
[48]Tamasloukht B,Wong Q uai Lam M S,Martinez Y,et al.Characterization of a cinnamoyl-CoA reductase 1(CCR 1)mutant in maize:effects on lignification,fibre development,and global gene expression[J].Joumal of Experimental Botany,2011,62(11):3837-3848.
[49]胡可,严雪锋,栗丹,等.沉默CCR和CAD基因培育低木质素含量转基因多年生黑麦草[J].草业学报,2013,22(05):72-83.
[50]Thevenin J,Pollet B,Letarnec B.The simultaneous repression of CCR and CAD,two enzymes of the lignin biosynthetic pathway,results in sterility and dwarfism in Arabidopsis thaliana[J].Molecular Plant,2011,4(1):70-82.
[51]孔华,郭安平,郭运玲,等.木质素生物合成及转基因调控研究进展[J].热带农业工程,2009,33(5):47-53.
[52]Martínez-García P J.Crepeau M W,Puiu D,et al.The walnut(Juglans regia)genome sequence reveals diversity in genes coding for the biosynthesis of non-structural polyphenols[J].Plant J,2016,87(5):507 532.