复方丹参注射液对大肠埃希菌致小鼠睾丸细胞凋亡的作用
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摘要
目的建立雄性小鼠生殖系统的大肠埃希菌逆行性感染动物模型,探讨复方丹参注射液对该模型中睾丸细胞凋亡的抑制作用。方法建立雄性小鼠生殖系统的大肠埃希菌逆行性感染动物模型后,腹腔注射复方丹参注射液,7 d后观察小鼠的精子数量、精子成活率、精子畸形率的改变;用TUNEL方法检测睾丸组织的凋亡指数;用Western blot检测Fas和Fasl的表达水平。结果高剂量的复方丹参注射液,可明显升高大肠埃希菌生殖系统感染小鼠的精子数量、精子成活率,降低精子畸形率;而且睾丸的细胞凋亡指数、Fas和Fasl的表达水平也明显降低。结论复方丹参注射液可抑制大肠埃希菌生殖系统逆行感染后睾丸细胞的凋亡,并对睾丸的生精功能具有保护作用。
Objective To investigate the inhibitory effect of compound salvia injection on apoptosis of testis induced by escherichia coli retrograde infection in mice.Methods After the model of retrograde infection of escherichia coli in genital system of mice was established,compound salvia injection was intraperitoneally injected.The amount of sperms,survival rate of sperms and rate of sperm deformation were observed on 7thday.Apoptotic index in testis was detected by TUNEL,and the expressions of Fas and Fasl was analyzed by Western blot.Results After the genital system of mice was infected by escherichia coli,compound salvia injection obviously increased the amount and survival rate of sperms,and reduced the rate of sperm deformation,as well as decreased apoptotic index and the levels of Fas and Fasl.Conclusion Compound salvia injection can inhibit the apoptosis of testis induced by escherichia coli retrograde infection in mice,and protect the spermatogenic function of testis.
Objective To investigate the inhibitory effect of compound salvia injection on apoptosis of testis induced by escherichia coli retrograde infection in mice.Methods After the model of retrograde infection of escherichia coli in genital system of mice was established,compound salvia injection was intraperitoneally injected.The amount of sperms,survival rate of sperms and rate of sperm deformation were observed on 7thday.Apoptotic index in testis was detected by TUNEL,and the expressions of Fas and Fasl was analyzed by Western blot.Results After the genital system of mice was infected by escherichia coli,compound salvia injection obviously increased the amount and survival rate of sperms,and reduced the rate of sperm deformation,as well as decreased apoptotic index and the levels of Fas and Fasl.Conclusion Compound salvia injection can inhibit the apoptosis of testis induced by escherichia coli retrograde infection in mice,and protect the spermatogenic function of testis.
引文
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[11]陈建珍,叶蓓.复方丹参注射液对大鼠脑缺血再灌注损伤细胞凋亡的影响[J].中国老年学杂志,2011,31(14):2711-2733.
[12]蒋赞利,茅祖斌,吴小涛,等.复方丹参注射液对脊髓损伤大鼠细胞凋亡的影响[J].中国临床康复,2003,7(23):3182-3183.
[13]黄清春,黄伟毅,沈鹰.复方丹参注射液对CIA大鼠滑膜细胞凋亡及关节功能的影响[J].现代康复,2011,5(10):58-59.
[2]蒋红梅,逯惠敏,刘莲.复方丹参注射液抗睾丸缺血再灌注损伤的实验研究[J].时珍国医国药,2010,21(1):85-86.
[3]黄霆,王林辉,孙颖浩.头孢他啶联合复方丹参片治疗急性附睾炎32例[J].实用医学杂志,2011,27(3):479-482.
[4]王庆银,姚庆强.丹参提取液体外抑菌活性研究[J].食品与药品,2010,12(5):184-187.
[5]朱嘉蓉,罗厚蔚.丹参酮ⅡA的抑菌活性研究[J].中国药科大学学报,2004,35(4):368-370.
[6]于爱莲,李忠培,邓文,等.大肠埃希菌感染诱导小鼠生精细胞凋亡的研究[J].中国病原生物学杂志,2008,3(12):889-902.
[7]Fowler JE Jr,Mariano M.Bacterial infection and male infertility:absence of immunoglobulin A with specificity for common Escherichia coli 0-serotypes in seminal fluid of infertile men[J].J Urol,1983,130(1):171-174.
[8]Lee EW,Seo J,Jeong M,et al.The roles of FADD in extrinsic apoptosis and necroptosis[J].BMB Rep,2012,45(9):496-508.
[9]Celik-Ozenci C,Sahin Z,Ustunel I,et al.The Fas system may have a role in male reproduction[J].Fertil Steril,2006,85(Suppl 1):1168-1178.
[10]陈永新,吕林,赵玉慧,等.复方丹参注射液联合黄芪注射液治疗充血性心力衰竭的临床观察[J].中国中医基础医学杂志,2005,11(6):468.
[11]陈建珍,叶蓓.复方丹参注射液对大鼠脑缺血再灌注损伤细胞凋亡的影响[J].中国老年学杂志,2011,31(14):2711-2733.
[12]蒋赞利,茅祖斌,吴小涛,等.复方丹参注射液对脊髓损伤大鼠细胞凋亡的影响[J].中国临床康复,2003,7(23):3182-3183.
[13]黄清春,黄伟毅,沈鹰.复方丹参注射液对CIA大鼠滑膜细胞凋亡及关节功能的影响[J].现代康复,2011,5(10):58-59.