泌尿生殖道支原体采用固体联合液体培养新方案的实验研究
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摘要
目的探讨支原体采用固体联合液体培养的最优组合方案。方法收集2016年3月~6月泌尿生殖道标本961例,采用固体(培养基)与液体(培养液)平行培养支原体,48 h后判读结果,筛选假阳性培养液,取20μl转种固体培养,48 h后再次判读结果。统计分析数据,对比固体与液体不同组合培养方案的优缺点。结果原代培养中固体培养阳性率UU为38.7%(372/961),MH为7.8%(75/961);液体培养阳性率UU为45.27%(435/961),MH为7.08%(68/961)。两种方法培养UU差异率为9.47%(91/961),差异有统计学意义(X~2=43.61,P<0.05);MH差异率为3.02%(29/961),差异无统计学意义(X~2=1.24,P>0.05)。转种固体培养与原代固体培养差异率为53.5%(46/86)。总体(原代+转种)固体培养阳性率UU为41.9%(403/961),MH为8.6%(83/961);与单次(原代或转种)固体培养相比,敏感度高。总体固体培养与单独液体培养的差异率UU为6.24%(60/961),MH为3.43%(33/961),差异有统计学意义(UU:X~2=17.067,P<0.05;MH:X~2=5.94,P<0.05)。结论支原体采用"两种方法平行原代培养,继而假阳性转种固体培养,以总体固体培养为最终结果"的培养方案,兼顾固体培养特异度高的优点与液体培养敏感度高的优点,可以提高培养准确度。
Objective To investigate the best experimental scheme of Mycoplasma solid culture combined with liquid culture.Methods A total of 961 samples of urogenital tract excretion were collected from March 2016 to June 2016. Both solid culture and liquid culture were performed for detection of Mycoplasma,false positive broths were picked out after 48 h,20 μl of each one was transformed to solid media for subculture, final results were read after 48 h. The experimental data was analyzed to find an optimal combination culture scheme. Results The positive rate of solid culture was 38. 7%(372/961) for UU and 7. 8%(75/961) for MH. The positive rate of liquid culture was 45. 27%(435/961) for UU and 7. 08%(68/961)for MH. The different rate between both methods was 9. 47%(91/961) for UU(X~2=43. 61,P<0. 005),and 3. 02%(29/961) for MH(X~2=1. 24,P>0. 05). The different rate was 53. 5%(46/86) between primary solid culture and subculture.The positive rate of total(primary solid culture and subculture) solid culture was 41. 9%(403/961) for UU and 8. 6%(83/961) for MH, which produced higher sensitivity than single(primary solid culture or subculture) solid culture. The different rate between the total solid culture and the liquid method was 6. 24%(60/961) for UU(X~2=17. 067,P<0. 05),and 3. 43%(33/961) for MH(X~2=5. 94,P<0. 05). Conclusion Perform both solid culture and liquid culture for Mycoplasma, pick out those false positive broths for subculture. Total solid culture could be more reliable as final result,for which could combine specificity of solid culture with sensitivity of liquid culture.
Objective To investigate the best experimental scheme of Mycoplasma solid culture combined with liquid culture.Methods A total of 961 samples of urogenital tract excretion were collected from March 2016 to June 2016. Both solid culture and liquid culture were performed for detection of Mycoplasma,false positive broths were picked out after 48 h,20 μl of each one was transformed to solid media for subculture, final results were read after 48 h. The experimental data was analyzed to find an optimal combination culture scheme. Results The positive rate of solid culture was 38. 7%(372/961) for UU and 7. 8%(75/961) for MH. The positive rate of liquid culture was 45. 27%(435/961) for UU and 7. 08%(68/961)for MH. The different rate between both methods was 9. 47%(91/961) for UU(X~2=43. 61,P<0. 005),and 3. 02%(29/961) for MH(X~2=1. 24,P>0. 05). The different rate was 53. 5%(46/86) between primary solid culture and subculture.The positive rate of total(primary solid culture and subculture) solid culture was 41. 9%(403/961) for UU and 8. 6%(83/961) for MH, which produced higher sensitivity than single(primary solid culture or subculture) solid culture. The different rate between the total solid culture and the liquid method was 6. 24%(60/961) for UU(X~2=17. 067,P<0. 05),and 3. 43%(33/961) for MH(X~2=5. 94,P<0. 05). Conclusion Perform both solid culture and liquid culture for Mycoplasma, pick out those false positive broths for subculture. Total solid culture could be more reliable as final result,for which could combine specificity of solid culture with sensitivity of liquid culture.
引文
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[2]Kim JK.Epidemiological trends of sexually transmitted infections among women in Cheonan,South Korea,2006~2012[J].J Microbiol Biotechnol,2013,23(10):1484-1490.
[3]张红升,韩晶.支原体液体培养法假阳性原因探讨[J].检验医学,2012,27(4):316-318.Zhang HS,Han J.The discussion of false positive of broth culture for Mycoplasma[J].Laboratory Medicine,2012,27(4):316-318.
[4]潘新娣,施前锋,邵伟芳.支原体属固体与液体培养相结合在临床感染诊断中的应用[J].中华医院感染学杂志,2013,23(4):949-951.Pan XD,Shi QF,Shao WF.Mycoplasma solid culture combined with liquid culture in clinic diagnosis of infections[J].Chin J Nosocomiol,2013,23(4):949-951.
[5]周运恒,马红霞,曹广亚,等.两种培养方法检测解脲脲原体及其菌落形态学的比较研究[J].检验医学,2013,28(5):362-365.Zhou YH,Ma HX,Cao GY,et al.Comparison study on the determination of colony morphology of Ureaplasma urealyticum by two kinds of culture media[J].Laboratory Medicine,2013,28(5):362-365.