摘要
提出了一种利用智能手机测定维生素B_(12)含量的分光光度法,利用智能手机光线感应器及相关软件"RGB调色板"和"phyphox"等对不同浓度维生素B_(12)的透光强度进行测量,数据处理后得到相应的吸光度值,发现其与维生素B_(12)的浓度在4.0~96.0μg/mL范围内呈现良好的线性关系。此方法具有简单、快捷等优点,可应用于药剂中维生素B_(12)含量的测定。
The method of determination of vitamin B_(12) in tablets was established by using two smart phones and the related apps "RGB palette"and "phyphox".The concentration of vitamin B_(12) could be measured through the light intensity and calculated the corresponding value of absorbance which could draw the calibration curve, the calibration curve was linear within the range of 4.0~96.0 μg/mL for vitamin B_(12).This method was simple and quick, which could be applied to the determination of vitamin B_(12) in pharmaceuticals.
引文
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