表皮生长因子和表皮生长因子受体与大鼠自然妊娠和早产的关系
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摘要
目的探讨表皮生长因子(EGF)和表皮生长因子受体(EGFR)与大鼠自然妊娠和早产的关系。方法将16只成年雌性SD大鼠分为中孕组、晚孕组、妊娠组和早产组,每组4只。比较4组血清和羊水的EGF水平及胎盘EGFR的相对表达量,并分析其相关性。结果妊娠组血清EGF水平均高于其他3组(均P <0. 05),而早产组和中孕组羊水EGF水平均低于晚孕组和妊娠组(均P <0. 05)。妊娠组胎盘EGFR相对表达量均高于其他3组(均P <0. 05)。血清EGF水平、羊水EGF水平及胎盘EGFR表达量三者之间均呈正相关(P <0. 05)。结论 EGF和EGFR水平均随着大鼠孕期延长逐渐升高,当EGF和EGFR水平在孕晚期出现下降,则可能预示着早产。EGF和EGFR可能可以作为自然妊娠的观测指标和预防早产的生物学标志物。
Objective To investigate the relationship between epidermal growth factor( EGF) and epidermal growth factor receptor( EGFR) with natural pregnancy and preterm birth in rats. Methods Sixteen adult female SD rats were divided into mid-pregnancy group,late-pregnancy group,pregnancy group,or premature group,with 4 rats in each group. The EGF levels of serum,amniotic fluid,and the relative expression level of placental EGFR were compared between four groups,and their correlations were analyzed. Results Serum EGF level was higher in pregnancy group than in the other three groups( all P < 0. 05),the EGF level of amniotic fluid was lower in the preterm group and mid-pregnancy group than in the late-pregnancy group and the pregnancy group,respectively( all P < 0. 05). The relative expression level of placental EGFR was higher in the pregnancy group than in the other three groups( all P < 0. 05).Positive correlation was found among the serum EGF level,EGF level of amniotic fluid and placental EGFR( P < 0. 05).Conclusion EGF and EGFR levels gradually increase with gestation prolonging,and decline of EGF and EGFR levels in the late-pregnancy probably indicates premature birth. Therefore,EGF and EGFR may be used as observation indices for natural pregnancy and biomarkers for preventing preterm birth.
Objective To investigate the relationship between epidermal growth factor( EGF) and epidermal growth factor receptor( EGFR) with natural pregnancy and preterm birth in rats. Methods Sixteen adult female SD rats were divided into mid-pregnancy group,late-pregnancy group,pregnancy group,or premature group,with 4 rats in each group. The EGF levels of serum,amniotic fluid,and the relative expression level of placental EGFR were compared between four groups,and their correlations were analyzed. Results Serum EGF level was higher in pregnancy group than in the other three groups( all P < 0. 05),the EGF level of amniotic fluid was lower in the preterm group and mid-pregnancy group than in the late-pregnancy group and the pregnancy group,respectively( all P < 0. 05). The relative expression level of placental EGFR was higher in the pregnancy group than in the other three groups( all P < 0. 05).Positive correlation was found among the serum EGF level,EGF level of amniotic fluid and placental EGFR( P < 0. 05).Conclusion EGF and EGFR levels gradually increase with gestation prolonging,and decline of EGF and EGFR levels in the late-pregnancy probably indicates premature birth. Therefore,EGF and EGFR may be used as observation indices for natural pregnancy and biomarkers for preventing preterm birth.
引文
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[14] Allen WR,Gower S,Wilsher S. Localisation of epidermal growth factor(EGF),its specific receptor(EGF-R)and aromatase at the materno-fetal interface during placentation in the pregnant mare[J]. Placenta,2017,50:53-59.
[15] Armant DR,Fritz R,Kilburn BA,et al. Reduced expression of the epidermal growth factor signaling system in preeclampsia[J]. Placenta,2015,36(3):270-278.
[16] Villanueva-García D,Wang KT,Nielsen HC,et al. Expression of specific protein kinase C(PKC)isoforms and ligand-specific activation of PKCalpha in late gestation fetal lung[J]. Exp Lung Res,2007,33(3/4):185-196.
[17]刘洪梅,曹云霞.表皮生长因子及其受体与早期自然流产相关性研究[J].安徽预防医学杂志,2006,12(3):161-163.
[18]何国珍.表皮生长因子与难免流产的关系探讨[J].广西中医学院学报,2005,8(3):22-23.
[19]王少为,李巨,尚立新,等.表皮生长因子及其受体与流产关系的探讨[J].中国实用妇科与产科杂志,1997,13(5):285-286.
[20] Cui L,Shu C,Liu Z,et al. The expression of serum s EGFR,s Flt-1,s Endoglin and PLGF in preeclampsia[J]. Pregnancy Hypertens,2018,13:127-132.
[2]温岩,孙晓艳,孙晓峰.多指标联合筛查在早产预测中的应用及评价[J].中国实验诊断学,2011,15(3):433-435.
[3] Haram K,Mortensen JH,Wollen AL. Preterm delivery:an overview[J]. Acta Obstet Gynecol Scand,2003,82(8):687-704.
[4] Martin JA,Hamilton BE,Ventura SJ,et al. Births:final data for 2011[J]. Natl Vital Stat Rep,2013,62(1):1-69,72.
[5]董微,张建迎,韩玉环.早产预测指标及其检查时机的研究[J].国际妇产科学杂志,2009,36(5):391-393,397.
[6]莫玉俏.早产预测指标分析的概况[J].医学综述,2012,18(23):3 991-3 993.
[7] H9gberg U. The world health report 2005:"make every mother and child count"-including Africans[J]. Scand J Public Health,2005,33(6):409-411.
[8] Howse JL. March of dimes commitment to solving the problem of prematurity[J]. Paediatr Perinat Epidemiol,2001,15(Suppl 2):1-2.
[9] Ma L,Wang AH,Frieda L,et al. Effect of epidermal growth factor and dexamethasone on fetal rat lung development[J]. Chin Med J(Engl),2009,122(17):2 013-2 016.
[10] Carpenter G. Epidermal growth factor:biology and mechanism of action[J]. Birth Defects Orig Artic Ser,1980,16(2):61-72.
[11] Fu YY,Gao WL,Chen M,et al. Prostasin regulates human placental trophoblast cell proliferation via the epidermal growth factor receptor signaling pathway[J]. Hum Reprod,2010,25(3):623-632.
[12] Hofmann GE,Abramowicz JS. Epidermal growth factor(EGF)concentrations in amniotic fluid and maternal urine during pregnancy[J]. Acta Obstet Gynecol Scand,1990,69(3):217-221.
[13] Takeuchi M,Seki M,Furukawa E,et al. Improvement of implantation potential in mouse blastocysts derived from IVF by combined treatment with prolactin,epidermal growth factor and 4-hydroxyestradiol[J]. Mol Hum Reprod,2017,23(8):557-570.
[14] Allen WR,Gower S,Wilsher S. Localisation of epidermal growth factor(EGF),its specific receptor(EGF-R)and aromatase at the materno-fetal interface during placentation in the pregnant mare[J]. Placenta,2017,50:53-59.
[15] Armant DR,Fritz R,Kilburn BA,et al. Reduced expression of the epidermal growth factor signaling system in preeclampsia[J]. Placenta,2015,36(3):270-278.
[16] Villanueva-García D,Wang KT,Nielsen HC,et al. Expression of specific protein kinase C(PKC)isoforms and ligand-specific activation of PKCalpha in late gestation fetal lung[J]. Exp Lung Res,2007,33(3/4):185-196.
[17]刘洪梅,曹云霞.表皮生长因子及其受体与早期自然流产相关性研究[J].安徽预防医学杂志,2006,12(3):161-163.
[18]何国珍.表皮生长因子与难免流产的关系探讨[J].广西中医学院学报,2005,8(3):22-23.
[19]王少为,李巨,尚立新,等.表皮生长因子及其受体与流产关系的探讨[J].中国实用妇科与产科杂志,1997,13(5):285-286.
[20] Cui L,Shu C,Liu Z,et al. The expression of serum s EGFR,s Flt-1,s Endoglin and PLGF in preeclampsia[J]. Pregnancy Hypertens,2018,13:127-132.