宫颈高级别病变和宫颈癌患者HPV感染状况及基因型分布
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摘要
目的分析宫颈高级别病变和宫颈癌患者HPV感染状况及基因型分布特点。方法采用PCR-反向点杂交法对200例宫颈高级别病变和宫颈癌患者进行HPV基因分型检测。结果检出HPV亚型共计20种,包括16种高危型HPV和4种低危型HPV。其中,HPV 16的感染最常见(55.50%),其次为HPV 52(17.00%)、HPV 58(17.00%)、HPV 33(10.50%)、HPV 18(8.00%)、HPV 31(4.50%)。HPV单一感染140例;其中,HPV 16为80例(57.14%),HPV 58为18例(12.86%)。宫颈上皮内瘤变(CIN)2、CIN3及宫颈癌患者的HPV单一感染率和多重感染率比较差异均无统计学意义(P>0.05)。结论宫颈高级别病变及宫颈癌患者HPV感染的主要亚型依次为HPV 16、HPV 52、HPV 58、HPV 33、HPV 18和HPV 31;九价疫苗对宫颈癌防控意义更大,但仍需扩大样本量进一步调查研究。
Objective To analyze the infection status and genotype distribution of human papillomavirus(HPV) in the patients with high-grade cervical lesions and cervical cancer.MethodsThe genotype of HPV in 200 patients with high-grade cervical lesions and cervical cancer was analyzed by PCR-reverse dot hybridization.Results A total of 20 HPV subtypes was detected,including 16 high-risk HPVs and 4 low-risk HPVs.Statistical analysis showed that HPV 16 was the main infection(55.50%),which was followed by HPV 52(17.00%),HPV 58(17.00%),HPV 33(10.50%),HPV 18(8.00%) and HPV 31(4.50%).Of 140 cases with single HPV infection,HPV 16 occurred in 80 cases(57.14%) and HPV 58 occurred in 18 cases(12.86%).There was no significant difference in the single and multiple infection among the patients with CIN2,CIN3 and cervical cancer(P>0.05).Conclusion The main subtypes of HPV infection in the patients with high-grade cervical lesions and cervical cancer were in an order of HPV 16>HPV 52>HPV 58>HPV 33>HPV 18>HPV 31.The 9-valent HPV vaccine may be important for the prevention and control of cervical cancer,but which needs extensive samples for further investigation.
Objective To analyze the infection status and genotype distribution of human papillomavirus(HPV) in the patients with high-grade cervical lesions and cervical cancer.MethodsThe genotype of HPV in 200 patients with high-grade cervical lesions and cervical cancer was analyzed by PCR-reverse dot hybridization.Results A total of 20 HPV subtypes was detected,including 16 high-risk HPVs and 4 low-risk HPVs.Statistical analysis showed that HPV 16 was the main infection(55.50%),which was followed by HPV 52(17.00%),HPV 58(17.00%),HPV 33(10.50%),HPV 18(8.00%) and HPV 31(4.50%).Of 140 cases with single HPV infection,HPV 16 occurred in 80 cases(57.14%) and HPV 58 occurred in 18 cases(12.86%).There was no significant difference in the single and multiple infection among the patients with CIN2,CIN3 and cervical cancer(P>0.05).Conclusion The main subtypes of HPV infection in the patients with high-grade cervical lesions and cervical cancer were in an order of HPV 16>HPV 52>HPV 58>HPV 33>HPV 18>HPV 31.The 9-valent HPV vaccine may be important for the prevention and control of cervical cancer,but which needs extensive samples for further investigation.
引文
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[10] 万晓春,杨慧娟,周晓燕,等.高危型HPV亚型及亚型组合检测宫颈癌及高级别宫颈上皮内瘤变的比较研究[J].中国癌症杂志,2014,24(5):342-348.
[2] 中华预防医学会妇女保健分会.子宫颈癌综合防控指南[M].北京:人民卫生出版社,2017:2-5.
[3] Wang YY,Li L,Wei S,et al.Human papillomavirus(HPV) infection in women participating in cervical cancer screening from 2006 to 2010 in Shenzhen City,South China[J].Asian Pac J Cancer Prev,2013,14(12):7483-7487.
[4] 赵颖.1454例女性感染高危型HPV基因亚型的分析[J].中国城乡企业卫生,2018,11(11):88-89.
[5] Ortiz A,Oliver G.On the use of the overlapping area matrix for image segmentation evaluation:a survey and new performance measures[J].Pattern Recogn Lett,2006,27(16):1916-1926.
[6] Peng RP,Li HM,Chang H,et al.Prevalence and genotype distribution of cervical human papillomavirus infection among female sex workers in Asia:a systematic literature review and meta-analysis[J].Sex Health,2012,9(2):113-119.
[7] 智艳芳,李肖甫,班振英,等.河南地区女性HPV感染及基因型分布研究[J].中华肿瘤防治杂志,2014,21(9):660-664.
[8] 杜宏,索兰草,刘红贤,等.甘肃地区女性宫颈HPV感染的现状研究[J].暨南大学学报(自然科学与医学版),2015,36(1):40-45.
[9] 冯余宽,彭英,朱联,等.四川地区HPV亚型及多重感染与宫颈癌前病变的关系初探[J].四川大学学报(医学版),2015,46(3):422-425.
[10] 万晓春,杨慧娟,周晓燕,等.高危型HPV亚型及亚型组合检测宫颈癌及高级别宫颈上皮内瘤变的比较研究[J].中国癌症杂志,2014,24(5):342-348.