抗骨髓炎片对家兔慢性骨髓炎的改善作用研究
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摘要
目的观察抗骨髓炎片(KST)对家兔慢性骨髓炎(CO)的改善作用。方法将金黄色葡萄球菌注入家兔右胫骨骨髓腔内建立CO模型,将CO家兔分为模型组、庆大霉素(60 mg/kg)组及KST高、低剂量(480、240 mg/kg)组,另设假手术组,ig给药。术后第2、4、6周对右胫骨行X-线检查并做诊断分型,采血进行白细胞计数,取血清检测C-反应蛋白(CRP)含量,术后第6周取右胫骨检测核因子κB(NF-κB)的阳性表达。结果 KST高、低剂量组术后第2、4、6周的X-片诊断分型与模型组比较均显著改善,有统计学意义(P<0.05、0.01);与模型组比较,KST高、低剂量组的白细胞计数、血清CRP含量、胫骨组织NF-κB表达在第2、4、6周均显著降低(P<0.05、0.01)。结论 KST对CO家兔具有保护作用,其机制可能与抑制致病菌、缓解炎症反应、下调炎性反应调控因子NF-κB等环节有关。
Objective To observe the protective effect of Kanggusuiyan Tablet(KST) against rabbits with chronic osteomyelitis(CO). Methods CO model of rabbit was achieved by intra-shinbone marrow injection of Staphylococcus aureus. CO rabbits were divided randomly into five groups, including sham group, model group, KST high and low dose(480 and 240 mg/kg, respectively)group and gentamicin(60 mg/kg) group. Drugs were administered orally daily for six weeks. The X-ray of right shinbone in rabbits were evaluated and graded, leucocyte count and level of C-reactive protein(CRP) in serum were examined on the 2nd, 4th and 6th week post-injection, respectively. At the end of the experiment, all the rabbits were sacrificed and the expression of NF-κB in right shinbone was determined by immunohistochemistry. Results The grading of X-ray in KST groups(480 and 240 mg/kg) were significantly improved compared with that of model group on the 2nd, 4th and 6th week, respectively(P < 0.05 or P < 0.01).Comparing to those of model group, leucocyte count and sera CRP levels in KST groups were significantly reduced on the 2nd, 4th and 6th week, respectively(P < 0.01). KST(480 and 240 mg/kg) significantly decreased the expression of NF-κB in shinbone compared with that of model group(P < 0.01). Conclusion The protective effect of KST against CO seems to be associated with inhibition of pathogenic bacteria, improvement of inflammation and downregulation of NF-κB, etc.
Objective To observe the protective effect of Kanggusuiyan Tablet(KST) against rabbits with chronic osteomyelitis(CO). Methods CO model of rabbit was achieved by intra-shinbone marrow injection of Staphylococcus aureus. CO rabbits were divided randomly into five groups, including sham group, model group, KST high and low dose(480 and 240 mg/kg, respectively)group and gentamicin(60 mg/kg) group. Drugs were administered orally daily for six weeks. The X-ray of right shinbone in rabbits were evaluated and graded, leucocyte count and level of C-reactive protein(CRP) in serum were examined on the 2nd, 4th and 6th week post-injection, respectively. At the end of the experiment, all the rabbits were sacrificed and the expression of NF-κB in right shinbone was determined by immunohistochemistry. Results The grading of X-ray in KST groups(480 and 240 mg/kg) were significantly improved compared with that of model group on the 2nd, 4th and 6th week, respectively(P < 0.05 or P < 0.01).Comparing to those of model group, leucocyte count and sera CRP levels in KST groups were significantly reduced on the 2nd, 4th and 6th week, respectively(P < 0.01). KST(480 and 240 mg/kg) significantly decreased the expression of NF-κB in shinbone compared with that of model group(P < 0.01). Conclusion The protective effect of KST against CO seems to be associated with inhibition of pathogenic bacteria, improvement of inflammation and downregulation of NF-κB, etc.
引文
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[13]杨明炜,陆付耳,徐丽君,等.紫花地丁对耐甲氧西林金黄色葡萄球菌耐药质粒的消除作用[J].中西医结合研究, 2009, 1(1):27-28.
[14]陈柳萌,曹树稳,余燕影.白花蛇舌草-半枝莲药对提取物抑菌活性部位研究[J].时珍国医国药, 2011, 22(1):93-95.
[15]郑险峰,张金艳,邵淑娟,等.白头翁对金黄色葡萄球菌和产气肠杆菌抑菌作用的研究[J].牡丹江师范学院学报:自然科学版, 2011,(2):29-30.
[16] Lin K H, Wang F L, Wu M S, et al. Serum procalcitonin and C-reactive protein levels as markers of bacterial infection in patients with liver cirrhosis:a systematic review and meta-analysis[J]. Diagn Microbiol Infect Dis,2014, 80(1):72-78.
[17] Pasparakis M. Role of NF-κB in epithelial biology[J].Immunol Rev, 2012, 246(1):346-358.
[18] Shandley S, Matthews K P, Cox J, et al. Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis[J]. J Orthop Res, 2012, 30(2):203-208.
[19]黄磊,许长鹏,贾俊杰,等.核因子-κB必需调节蛋白结合区多肽在兔胫骨骨髓炎模型中的抗炎效应研究[J].中华创伤骨科杂志, 2014, 16(5):415-420.
[2] Koury K L, Hwang J S, Sirkin M. The antibiotic nail in the treatment of long bone infection:technique and results[J]. Orthop Clin North Am, 2017, 48(2):155-165.
[3]韩宗其,左国营,郝小燕.中药成分联合抗生素抗MRSA作用的研究进展[J].中草药, 2012, 43(12):187-195.
[4]顾卓珺,顾维凤,吴飞跃,等.医院感染病原菌中药与抗菌药物联合治疗的研究[J].中华医院感染学杂志,2015, 25(10):2236-2238.
[5]田殿兴.急慢性化脓性骨髓炎的临床初步研究[A]//2008年中医外科学术年会论文集[C].济南:2008.
[6] Strelkov N S, Kiryanov N A, Shklyaev P O, et al.Pathologic morphology of acute experimental osteomyelitis[J]. Bosn J Basic Med Sci, 2013, 13(3):153-157.
[7]李素那,钱丽旗,郑彬丽,等.中药复方托毒生髓方治疗慢性骨髓炎的实验研究[J].感染·炎症·修复, 2012, 13(2):79-82.
[8] Kalinka J, Hachmeister M, Geraci J, et al.Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation[J]. Int J Med Microbiol, 2014, 304(8):1038-1049.
[9] Ulu?M, Ayaz C, Celen M K. A case report and literature review:osteomyelitis caused by community-associated methicillin resistant Staphylococcus aureus[J]. J Infect Dev Ctries, 2011, 5(12):896-900.
[10]刘振云.中医三联疗法治疗慢性骨髓炎200例[J].湖南中医杂志, 2014, 30(8):95-96.
[11]崔玉莹,王宪贝,李玉坤,等.金银花、连翘水煎液对金黄色葡萄球菌的抑制作用研究[J].光明中医, 2017, 32(18):2637-2638.
[12]霍佳楠,姜晓文,许晶,等.蒲公英水提物对体外金黄色葡萄球菌生物膜形成的影响[J].中兽医医药杂志,2015,(1):12-15.
[13]杨明炜,陆付耳,徐丽君,等.紫花地丁对耐甲氧西林金黄色葡萄球菌耐药质粒的消除作用[J].中西医结合研究, 2009, 1(1):27-28.
[14]陈柳萌,曹树稳,余燕影.白花蛇舌草-半枝莲药对提取物抑菌活性部位研究[J].时珍国医国药, 2011, 22(1):93-95.
[15]郑险峰,张金艳,邵淑娟,等.白头翁对金黄色葡萄球菌和产气肠杆菌抑菌作用的研究[J].牡丹江师范学院学报:自然科学版, 2011,(2):29-30.
[16] Lin K H, Wang F L, Wu M S, et al. Serum procalcitonin and C-reactive protein levels as markers of bacterial infection in patients with liver cirrhosis:a systematic review and meta-analysis[J]. Diagn Microbiol Infect Dis,2014, 80(1):72-78.
[17] Pasparakis M. Role of NF-κB in epithelial biology[J].Immunol Rev, 2012, 246(1):346-358.
[18] Shandley S, Matthews K P, Cox J, et al. Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis[J]. J Orthop Res, 2012, 30(2):203-208.
[19]黄磊,许长鹏,贾俊杰,等.核因子-κB必需调节蛋白结合区多肽在兔胫骨骨髓炎模型中的抗炎效应研究[J].中华创伤骨科杂志, 2014, 16(5):415-420.