5株植物根际促生菌对紫花苜蓿生长和品质的影响
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摘要
以前期研究中分离的5株植物根际促生菌(PGPR)为试验材料,采用盆栽试验的方式,将植物根际促生菌制剂接种到紫花苜蓿幼苗的土壤中,测定不同处理下苜蓿株高、茎粗等生长指标以及粗蛋白、粗脂肪和粗纤维等品质指标的变化,研究其对紫花苜蓿(Medicago sativa)生长和品质的影响。结果表明:选用的5株植物根际促生菌均能够有效促进紫花苜蓿生长,改善品质,但不同菌株的促生效果不同。促生菌株处理后,紫花苜蓿的株高、茎粗分别增加了11.2%~75.2%和4.1%~22.2%;粗蛋白、粗脂肪含量分别增加了6.0%~20.1%、3.8%~12.7%,酸性洗涤纤维(ADF)和中性洗涤纤维(NDF)含量分别降低了1.8%~3.8%、1.4%~4.4%。以菌株Gnyt1促生效果最佳,具有进一步开发利用的潜力。
Five plant rhizosphere growth promoting bacteria(PGPR)isolated from previous research were used as materials to study their effects on alfalfa growth and quality by pot experiment,and the plant height,stem diameter,and contents of crude protein,ether extract and crude fiber were determined under different treatments,in order to provide a basis for utilizing PGPR bacterial fertilizer to obtain high quality and high yield alfalfa in arid and semiarid areas.The results showed that these 5 PGPR strains could promote alfalfa growth and improve quality effectively.However,the effect of different strains varied.The plant height and stem diameter of alfalfa were increased by 11.2%to 75.2%and 4.1%to 22.2%respectively,and the contents of crude protein and ether extract were increased by 6.0%to 20.1%and 3.8%to 12.7%respectively.The contents of acid detergent fiber(ADF)and neutral detergent fiber(NDF)were decreased by1.8%to 3.8%,1.4%to 4.4%respectively.The strain Gnyt1 performed the best effect and shown the potential for further development and utilization.
Five plant rhizosphere growth promoting bacteria(PGPR)isolated from previous research were used as materials to study their effects on alfalfa growth and quality by pot experiment,and the plant height,stem diameter,and contents of crude protein,ether extract and crude fiber were determined under different treatments,in order to provide a basis for utilizing PGPR bacterial fertilizer to obtain high quality and high yield alfalfa in arid and semiarid areas.The results showed that these 5 PGPR strains could promote alfalfa growth and improve quality effectively.However,the effect of different strains varied.The plant height and stem diameter of alfalfa were increased by 11.2%to 75.2%and 4.1%to 22.2%respectively,and the contents of crude protein and ether extract were increased by 6.0%to 20.1%and 3.8%to 12.7%respectively.The contents of acid detergent fiber(ADF)and neutral detergent fiber(NDF)were decreased by1.8%to 3.8%,1.4%to 4.4%respectively.The strain Gnyt1 performed the best effect and shown the potential for further development and utilization.
引文
[1]刘芳,景戍旋,胡健,等.镉污染和接种丛枝菌根真菌对紫花苜蓿生长和氮吸收的影响[J].草业学报,2017,26(2):69-77.
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[15]韩华雯,孙丽娜,姚拓,等.苜蓿根际有益菌接种剂对苜蓿生长特性影响的研究[J].草地学报,2013,21(2):353-359.
[16]冯瑞章,姚拓,周万海,等.溶磷菌对燕麦生物量及植株氮、磷含量的影响[J].水土保持学报,2009,23(2):188-192.
[17]刘青海,姚拓,马从,等.6株溶磷菌和4株固氮菌混合培养条件的研究[J].草原与草坪,2011,31(6):1-6.
[18]李建宏.优良植物根际促生菌Bacillus mycoides Gnyt1特性研究及全基因组测序分析[D].兰州:甘肃农业大学,2017.
[19]王亚东.北京地区滴灌条件下不同灌水量对秋播紫花苜蓿冬前生长的影响[D].北京:北京林业大学,2016.
[20]甘肃农业大学.草原生态化学实验指导[M].北京:农业出版社,1987.
[21]马骢毓.民勤退耕区次生草地土壤微生物多样性研究及优势植物根际促生菌资源筛选[D].兰州:甘肃农业大学,2017.
[22]J W K loepper,C M Ryu,S Zhang.Rhizo-sphere bacteria help plants tolerate abiotic stress[J].Trends in plant Science,2009,14:1-4.
[23]李玉娥,姚拓,荣良燕.溶磷菌溶磷和分泌IAA特性及对苜蓿生长的影响[J].草地学报,2010,18(1):84-88.
[24]Molla M.Microbial mineralization of organic phosphate in soil[J].Plant and soil,1991,78:393-399.
[25]蒙海涛.不同秋眠类型苜蓿根系中内源激素含量动态研究[D].杨陵:西北农林科技大学,2008.
[26]Vessey J K,Heisinger K G.Effect of Penicillium bilaii inoculation and phosphorus fertilisation on root and shoot parameters of field-grown pea Canadian[J].Journal of Plant Science,2001,81(3):361-366.
[27]徐济春,林钊沐,罗微,等.矿质营养对光合作用影响的研究进展[J].安徽农学通报,2007(7):23-25.
[2]J Koher,F Caravaca,ARoldan.An AM fungus and a PGPR intensify the adverse effects of salinity of rhizosphere soil aggregates of Lactuca sativa[J].Soil Biology&biochemistry,2010,42:429-434.
[3]宁清同,王丽香.我国农业化肥污染防治制度初探[J].行政与法,2012(11):74-79.
[4]Malik K A,bilal R.Association of nitrogen-fixing plant growth promoting rhizobacterria(PGPR)with kallar grass and rice[J].Plant and soil,1997,94(2):37-34.
[5]农业部.到2020年化肥使用量零增长行动方案[Z].农发[2015]2号文件,2015-03-18.
[6]常娜,张雪娇,马璐璐,等.微生物菌剂对小麦生长及土传病害预防效果的影响[J].作物杂志,2017,35(1):155-160.
[7]管鹏.施用PGPR菌肥对不同土壤条件下的苜蓿生长及品质的影响[D].哈尔滨:哈尔滨师范大学,2014.
[8]纪忠东.微生物菌剂助力土壤环境的改善[J].营销界(农资与市场),2017(3):37-39.
[9]李丹.有机微生物菌肥对黄瓜生长发育、产量和品质的影响[J].现代园艺,2017(3):18
[10]张伟娜,朱先志,刘莉等.地恩地微生物菌肥对烤烟品种CF225产量及质量的影响[J].现代农业科技,2017(20):11-13.
[11]郑明燕.复合微生物液肥对小辣椒幼苗生长及产量的影响[D].郑州:河南农业大学,2009.
[12]刘青海.六株溶磷菌与四株固氮菌互作效应及其菌剂对苜蓿促生效果研究[D].兰州:甘肃农业大学,2011.
[13]马文彬,姚拓,王国基,等.根际促生菌筛选及其接种剂对箭筈豌豆生长影响的研究[J].草业学报,2014,23(5):241-248.
[14]葛诚.微生物肥料生产及其产业化[M].北京:化学工业出版社,2007.
[15]韩华雯,孙丽娜,姚拓,等.苜蓿根际有益菌接种剂对苜蓿生长特性影响的研究[J].草地学报,2013,21(2):353-359.
[16]冯瑞章,姚拓,周万海,等.溶磷菌对燕麦生物量及植株氮、磷含量的影响[J].水土保持学报,2009,23(2):188-192.
[17]刘青海,姚拓,马从,等.6株溶磷菌和4株固氮菌混合培养条件的研究[J].草原与草坪,2011,31(6):1-6.
[18]李建宏.优良植物根际促生菌Bacillus mycoides Gnyt1特性研究及全基因组测序分析[D].兰州:甘肃农业大学,2017.
[19]王亚东.北京地区滴灌条件下不同灌水量对秋播紫花苜蓿冬前生长的影响[D].北京:北京林业大学,2016.
[20]甘肃农业大学.草原生态化学实验指导[M].北京:农业出版社,1987.
[21]马骢毓.民勤退耕区次生草地土壤微生物多样性研究及优势植物根际促生菌资源筛选[D].兰州:甘肃农业大学,2017.
[22]J W K loepper,C M Ryu,S Zhang.Rhizo-sphere bacteria help plants tolerate abiotic stress[J].Trends in plant Science,2009,14:1-4.
[23]李玉娥,姚拓,荣良燕.溶磷菌溶磷和分泌IAA特性及对苜蓿生长的影响[J].草地学报,2010,18(1):84-88.
[24]Molla M.Microbial mineralization of organic phosphate in soil[J].Plant and soil,1991,78:393-399.
[25]蒙海涛.不同秋眠类型苜蓿根系中内源激素含量动态研究[D].杨陵:西北农林科技大学,2008.
[26]Vessey J K,Heisinger K G.Effect of Penicillium bilaii inoculation and phosphorus fertilisation on root and shoot parameters of field-grown pea Canadian[J].Journal of Plant Science,2001,81(3):361-366.
[27]徐济春,林钊沐,罗微,等.矿质营养对光合作用影响的研究进展[J].安徽农学通报,2007(7):23-25.