我国大米碳氮稳定同位素比率特征及溯源应用
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摘要
利用元素分析仪-稳定同位素比率质谱仪检测我国由北到南6个省的10种大米的δ13C和δ15N值。结果显示,我国大米的δ13C值为-28.3‰～-26.0‰,平均值为-27.0±0.8‰,以-27.9‰～-26.3‰出现的频率较高。δ15N值为1.1‰～4.1‰,均值为2.4±1.0‰,以1.7‰～3.4‰出现的频率较高。我国南方大米的δ13C值低于北方大米,但南北方大米的δ15N均值基本相同且小于4‰,符合施化肥大米的特征。根据国内外大米的δ13C和δ15N特异性,以δ13C和δ15N为统计变量,采用Q型系统聚类方法对大米归类溯源。欧式距离<4时,日本大米的3个群集与我国大米的1个群集区分明显;欧式距离<3时,美国大米的2个群集与我国大米的1个群集区分显著。基于大米的δ13C和δ15N特性能够对其产地进行溯源。
引文
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[3]CHUNG I M,KIM J K,PRABAKARAN M,et al.Authenticity of rice(Oryza sativa L.)geographical origin based on analysis of C,N,O and S stable isotope ratios:a preliminary case report in Korea,China and Philippine[J].Journal of the Science of Food and Agriculture,2016,96(7):2 433-2 439.
[4]SUZUKI Y,CHIKARAISHI Y,OGAWA N O,et al.Geographical origin of polished rice based on multiple element and stable isotope analyses[J].Food Chemistry,2008,109(2):470-475.
[5]KORENAGA T,MUSASHI M,NAKASHITA R,et al.Statistical analysis of rice samples for compositions of multiple light elements(H,C,N and O)and their stable isotopes[J].Analytical Sciences,2010,26(8):873-878.
[6]CHEN T,ZHAO Y,ZHANG W,et al.Variation of the light stable isotopes in the superior and inferior grains of rice(Oryza sativa L.)with different geographical origins[J].Food Chemistry,2016,209:95-98.
[7]邵圣枝,陈元林,张永志,等.稻米中同位素与多元素特征及其产地溯源PCA-LDA判别[J].核农学报,2015,29(1):119-127.
[8]CHUNG I M,KIM J K,LEE K J,et al.Geographic authentication of Asian rice(Oryza sativa L.)using multielemental and stable isotopic data combined with multivariate analysis[J].Food Chemistry,2018,240:840-849.
[9]KUKUSAMUDE C,KONGSRI S.Elemental and isotopic profiling of Thai jasmine rice(Khao Dawk Mali 105)for discrimination of geographical origins in Thung Kula Rong Hai area,Thailand[J].Food Control,2018,91:357-364.
[10]MARIOTTI A.Atmospheric nitrogen is a reliable standard for natural15N abundance measurements[J].Nature,1983,303:685-687.
[11]隋方功,王运华.植物碳素营养研究中碳同位素示踪技术的应用及进展[J].莱阳农学院学报,2001,18(2):107-111.
[12]刘贤赵,张勇,宿庆,等.现代陆生植物碳同位素组成对气候变化的响应研究进展[J].地球科学进展,2014,29(12):1 341-1 354.
[13]王国安,韩家懋.C3植物碳同位素在旱季和雨季中的变化[J].海洋地质与第四纪地质,2001,21(4):43-47.
[14]ROGERS K M.Nitrogen isotopes as a screening tool to determine the growing regimen of some organic and nonorganic supermarket produce from New Zealand[J].Journal of Agricultural and Food Chemistry,2008,56(11):4 078-4 083.
[15]BATEMAN A S,KELLY S D,WOOLFE M.Nitrogen isotope composition of organically and conventionally grown crops[J].Journal of Agricultural&Food Chemistry,2007,55(7):2 664-2 670.
[16]CHOI W J,ARSHAD M A,CHANG S X,et al.Grain15N of crops applied with organic and chemical fertilizers in a four year rotation[J].Plant and Soil,2006,284(1-2):165-174.
[17]CRAINE J M,ELMORE A J,AIDARR M P M,et al.Global patterns of foliar nitrogen isotopes and their relationships with climate,mycorrhizal fungi,foliar nutrient concentrations,and nitrogen availability[J].New Phytologist,2009,183(4):980-992.
[18]MARTINELLI L A,PICCOLO M C,TOWNSEND AR,et al.Nitrogen stable isotopic composition of leaves and soil:tropical versus temperate forests[J].Biogeochemistry,1999,46(1):45-65.!!!!!!!!!!!!!!!!!!!!!!!