ICP-MS法测定鲜冬虫夏草中5种重金属的含量
|
摘要
目的:建立一种同时测定鲜冬虫夏草样品中铅、镉、砷、汞、铜5种重金属残留量的分析方法。方法:样品制备采用微波消解法,样品测定采用电感耦合等离子体质谱(ICP-MS)法。结果:5个重金属元素在各自质量浓度范围内线性关系良好(r>0.996);平均加样回收率为99.55%～121.73%(RSD <4.3%);所测15批鲜冬虫夏草(繁育品)样品中5种重金属含量均符合中国、美国和欧洲药典标准要求。结论:建立的方法准确可靠,可用于鲜冬虫夏草中5种重金属元素的含量测定。
Objective:To establish a method for determination of five heavy metal elements(Pb,Cd,As,Hg and Cu)in fresh Cordyceps by ICP-MS.Methods:The fresh Cordyceps sample solution was prepared by microwave digestion.The contents of five heavy metal elements were assayed by ICP-MS.Results:The five elements standard curves revealed a good linear relationship in test ranges(r>0.996).The recoveries were in the range of 99.55%～121.73%(RSD<4.3%).The contents of five heavy metal elements in 15 batches cultivated fresh Cordyceps were comply with Chinese pharmacopoeia,United States pharmacopoeia and European pharmacopoeia.Conclusion:The developed method is accurate and reliable,which can be used to assay five heavy metal elements in fresh Cordyceps.
Objective:To establish a method for determination of five heavy metal elements(Pb,Cd,As,Hg and Cu)in fresh Cordyceps by ICP-MS.Methods:The fresh Cordyceps sample solution was prepared by microwave digestion.The contents of five heavy metal elements were assayed by ICP-MS.Results:The five elements standard curves revealed a good linear relationship in test ranges(r>0.996).The recoveries were in the range of 99.55%～121.73%(RSD<4.3%).The contents of five heavy metal elements in 15 batches cultivated fresh Cordyceps were comply with Chinese pharmacopoeia,United States pharmacopoeia and European pharmacopoeia.Conclusion:The developed method is accurate and reliable,which can be used to assay five heavy metal elements in fresh Cordyceps.
引文
[1]国家药典委员会.中华人民共和国药典[M].一部.北京:中国医药科技出版社,2015:115.
[2]钱正明,李春红,李文庆,等.在线提取HPLC法测定两种虫草中腺苷和虫草素的含量[J].今日药学,2018,28(6):387-390.
[3]徐月启,张强.中药冬虫夏草的性状及显微鉴定研究[J].亚太传统医药,2014,10(11):10-11.
[4]郑依玲,梅全喜,李文佳,等.冬虫夏草的药用历史及现代服用方法探讨[J].中药材,2017,40(11):2722-2725.
[5]肖瑛,胡雪峰,陶盛昌,等.鲜冬虫夏草药理作用研究进展[J].亚太传统医药,2018,14(4):80-85.
[6]钱正明,李春红,李文庆,等.冬虫夏草蛋白图谱及干燥条件对超氧化物歧化酶活性影响[J].菌物学报,2016,35(4):424-432.
[7]云南省食品药品监督管理局.云南省中药饮片炮制规范[S].昆明:云南科技出版社,1986:297-298.
[8]四川省食品药品监督管理局.四川省中药饮片炮制规范[S].成都:四川人民出版社,2015:96-97.
[9]赵安琪.鲜药材的生意经[J].中国药店,2015(18):50.
[10]王钢力,金红宇,韩小萍,等.冬虫夏草药材的质量研究及存在问题[J].中草药,2008,39(1):115-118.
[11]刘杰,李耀磊,昝珂,等.冬虫夏草人工繁育品和野生冬虫夏草中5种重金属及有害元素含量的比较[J].中国药事,2016,30(9):912-918.
[12]周利,郝庆秀,王升,等.微波消解ICP-MS法对冬虫夏草不同部位5种重金属元素的分布研究[J].中国中药杂志,2017,42(15):2934-2938.
[13]彭柳,范开静,熊清平.牛黄解毒片重金属限量标准研究[J].亚太传统医药,2013,9(4):32-34.
[14]谢凯,杨洋,薛汝增,等.ICP-OES法测定薄荷脑中重金属元素的含量[J].今日药学,2018,28(6):379-381.
[15]姜涛,施枝江,陈林明,等.ICP-MS法测定枸杞子5种金属元素的含量分析[J].亚太传统医药,2018,14(8):41-44.
[16]国家药典委员会.中华人民共和国药典[M].四部.北京:中国医药科技出版社,2015:205-207.
[17]韩小丽,张小波,郭兰萍,等.中药材重金属污染现状的统计分析[J].中国中药杂志,2008,33(18):2041-2048.
[18]郭伟.中药中的重金属及其检测和去除方法[J].天津中医药,2010,27(4):351-352.
[19]郭兰萍,周利,王升,等.《中医药-中药材重金属限量》ISO国际标准下中药材重金属污染现状与分析[J].科技导报,2017,35(11):91-98.
[20]THE UNITED STATES PHARMACOPEIAL CONVEN-TIOn.USP40-NS35[S].2016:440.
[21]EDQM.EP9.0[S].Strasbourg:Council of Europe,2017:819.
[2]钱正明,李春红,李文庆,等.在线提取HPLC法测定两种虫草中腺苷和虫草素的含量[J].今日药学,2018,28(6):387-390.
[3]徐月启,张强.中药冬虫夏草的性状及显微鉴定研究[J].亚太传统医药,2014,10(11):10-11.
[4]郑依玲,梅全喜,李文佳,等.冬虫夏草的药用历史及现代服用方法探讨[J].中药材,2017,40(11):2722-2725.
[5]肖瑛,胡雪峰,陶盛昌,等.鲜冬虫夏草药理作用研究进展[J].亚太传统医药,2018,14(4):80-85.
[6]钱正明,李春红,李文庆,等.冬虫夏草蛋白图谱及干燥条件对超氧化物歧化酶活性影响[J].菌物学报,2016,35(4):424-432.
[7]云南省食品药品监督管理局.云南省中药饮片炮制规范[S].昆明:云南科技出版社,1986:297-298.
[8]四川省食品药品监督管理局.四川省中药饮片炮制规范[S].成都:四川人民出版社,2015:96-97.
[9]赵安琪.鲜药材的生意经[J].中国药店,2015(18):50.
[10]王钢力,金红宇,韩小萍,等.冬虫夏草药材的质量研究及存在问题[J].中草药,2008,39(1):115-118.
[11]刘杰,李耀磊,昝珂,等.冬虫夏草人工繁育品和野生冬虫夏草中5种重金属及有害元素含量的比较[J].中国药事,2016,30(9):912-918.
[12]周利,郝庆秀,王升,等.微波消解ICP-MS法对冬虫夏草不同部位5种重金属元素的分布研究[J].中国中药杂志,2017,42(15):2934-2938.
[13]彭柳,范开静,熊清平.牛黄解毒片重金属限量标准研究[J].亚太传统医药,2013,9(4):32-34.
[14]谢凯,杨洋,薛汝增,等.ICP-OES法测定薄荷脑中重金属元素的含量[J].今日药学,2018,28(6):379-381.
[15]姜涛,施枝江,陈林明,等.ICP-MS法测定枸杞子5种金属元素的含量分析[J].亚太传统医药,2018,14(8):41-44.
[16]国家药典委员会.中华人民共和国药典[M].四部.北京:中国医药科技出版社,2015:205-207.
[17]韩小丽,张小波,郭兰萍,等.中药材重金属污染现状的统计分析[J].中国中药杂志,2008,33(18):2041-2048.
[18]郭伟.中药中的重金属及其检测和去除方法[J].天津中医药,2010,27(4):351-352.
[19]郭兰萍,周利,王升,等.《中医药-中药材重金属限量》ISO国际标准下中药材重金属污染现状与分析[J].科技导报,2017,35(11):91-98.
[20]THE UNITED STATES PHARMACOPEIAL CONVEN-TIOn.USP40-NS35[S].2016:440.
[21]EDQM.EP9.0[S].Strasbourg:Council of Europe,2017:819.