新疆昆仑雪菊水提物对小鼠肠道菌群的调节作用
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摘要
目的 研究新疆昆仑雪菊水提物对小鼠肠道菌群的影响。方法 C57BL/6小鼠,雄性,8周龄,40只,分为4个组(每组10只):高、中、低三个剂量组和空白对照组,给药14 d,收集给药前后小鼠粪样,16S rDNA实时荧光定量PCR法测定粪样中肠杆菌、肠球菌、乳杆菌和产气荚膜梭菌水平。结果 与空白对照组比较,中剂量组小鼠肠道粪样中乳杆菌显著增多(t=-2.503,P<0.05),低剂量组与高剂量组变化不显著;与空白对照组比较,其他组小鼠肠道中肠杆菌属、肠球菌属和产气荚膜梭菌水平均无显著变化。结论 参照《保健食品检验与评价技术规范—2003版》之"调节肠道菌群作用检验方法",新疆昆仑雪菊水提物对小鼠肠道菌群具有一定的调节作用。
Objective To investigate the influence of aqueous extract of Coriopsis ticntoria flowers from Xinjiang Kunlun Mountain on intestinal microflora in mice. Methods The mice were divided into the high dose group, medium dose group, low dose group and blank control group. Fecal samples were collected after 14 days of treatment. The 16 S rDNA real time quantitative PCR was used to detect the levels of Enterobacteriaceae, Enterococci, Lactobacilli and Clostridium perfringens. Results Compared with blank control group, the level of Lactobacilli in medium dose group increased significantly(t=-2.503, P<0.05). Conclusion The aqueous extract of Xinjiang Kunlun Coriopsis ticntoria flowers has a regulatory effect on the intestinal flora of mice.
Objective To investigate the influence of aqueous extract of Coriopsis ticntoria flowers from Xinjiang Kunlun Mountain on intestinal microflora in mice. Methods The mice were divided into the high dose group, medium dose group, low dose group and blank control group. Fecal samples were collected after 14 days of treatment. The 16 S rDNA real time quantitative PCR was used to detect the levels of Enterobacteriaceae, Enterococci, Lactobacilli and Clostridium perfringens. Results Compared with blank control group, the level of Lactobacilli in medium dose group increased significantly(t=-2.503, P<0.05). Conclusion The aqueous extract of Xinjiang Kunlun Coriopsis ticntoria flowers has a regulatory effect on the intestinal flora of mice.
引文
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[2] Wang Z,Klipfell E,Bennett BJ,et al.Gut flora metabolism of phosphatidylcholine promotes cardiovascula disease[J].Nature,2011,472(7341):57-63.
[3] Gupta SS,Mohammed MH,Ghosh TS,et al.Metagenome of the gut of a malnourished child[J].Gut Pathogens,2011,3(1):7-15.
[4] Chen X,D′Souza R,Hong ST.The role of gut microbiota in the gut-brain axis:Current challenges and perspectives[J].Protein & Cell,2013,4(6):403-414.
[5] Fei N,Zhao LP.An opportunistic pathogen isolated from the gut of an obese human causes obesity in germfree mice[J].ISME J,2013,7(4):880-884.
[6] Wang Y,Luo X,Mao X,et al.Gut microbiome analysis of type 2 diabetic patients from the Chinese minority ethnic groups the Uygurs and Kazaks[J].PLoS One,2017,12(3):1-15.
[7] ZHAO Haixia,LI Linlin,MAO Xinmin,et al.The relationship of Veillonella spp and C.coccoides subgroup levels in gut microbiota with type 2 diabetes mellitus in Uygurs and Kazaks of Xinjiang[J].Chin J Microecol,2015,27(1):6-9.(in Chinese) 赵海霞,李琳琳,毛新民,等.新疆维、哈两民族粪样中韦荣球菌属和梭菌属水平与2型糖尿病的相关性研究[J].中国微生态学杂志,2015,27(1):6-9.
[8] XIONG Jianhui,LI Linlin,WANG Ye,et al.The relationship of Atopobium cluster and Collinsella aerofaciens levels in gut microbiota with type 2 diabetes mellitus in Uygurs and Kazaks of Xinjiang[J].Chin J Microecol,2015,27(6):633-637.(in Chinese) 熊健会,李琳琳,王烨,等.新疆维、哈两民族粪样中奇异菌簇和产气柯林斯菌属水平与2型糖尿病的相关性研究[J].中国微生态学杂志,2015,27(6):633-637.
[9] QIN Le,XING Baiying.Progress on chemical constituents and pharmaceutical effects of Coreopsis tinctoria[J].Chem Indust Times,2017,31(4):36-38.(in Chinese) 秦乐,邢柏颖.昆仑雪菊化学成分及药理作用研究进展[J].化工时刊,2017,31(4):36-38.
[10] ZHANG Wenguang,LI Linlin,WANG Ye,et al.Effects of extracts from Xinjiang Kunlun Coreopsis tinctoria flowers on blood glucose in diabetic mice[J].Chin J Drug Appl & Monit,2015(2):82-84.(in Chinese) 张文广,李琳琳,王烨,等.新疆昆仑雪菊提取物对糖尿病小鼠血糖的影响[J].中国药物应用与检测,2015(2):82-84.
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[13] LI Yun,ZHOU Mingmei,GOU Xiaojun,et al.Effects of curcumin on gut microbiota of interval sleep deprivation rats[J].Chin Herbal Med,2016,47 (5):794-798.(in Chinese) 李云,周明眉,苟小军,等.姜黄素对间歇性睡眠剥夺大鼠特定肠道菌的影响[J].中草药,2016,47(5):794-798.
[14] GE Shaoyang,WANG Na,LIU Zhiling,et al.Effect of Bifidobacterium A6 on intestinal flora in healthy young people[J].China Dairy Cattle,2017(1):52-58.(in Chinese) 葛绍阳,王娜,刘治麟,等.双歧杆菌A6对健康青年人群肠道菌群的影响[J].中国奶牛,2017(1):52-58.
[15] Schwarzer M,Makki K,Storelli G,et al.Lactobacillus plantarum strain maintains growth of infant mice during chronic undernutrition[J].Science,2016,351(6275):854-857.
[16] Panigrahi P,Parida S,Nanda NC,et al.Corrigendum:A randomized synbiotic trial to prevent sepsis among infants in rural India[J].Nature,2017,548(7668):407.
[17] Sung V,D′Amico F,Cabana MD,et al.Lactobacillus reuteri to treat infant colic:A Meta-analysis[J].Pediatrics,2018,141(1):1-10.
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