苹果糖转运蛋白基因MdSWEET1在番茄中异源表达提高其耐盐性
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摘要
克隆得到苹果糖转运蛋白SWEET(sugars will eventually be exported transporters)基因MdSWEET1(MDP0000237435),组织表达分析发现该基因主要在苹果的茎和花中表达,相对定量分析发现其对NaCl、PEG、H_2O_2和ABA等均有响应。在番茄中异位表达MdSWEET1可提高植株耐盐性,并且可溶性糖含量,特别是蔗糖和果糖含量提高。
Sugars are important energy source and signaling molecule in plant,and play important roles in plant growth and development as well as biotic and abiotic stresses. Sugar cannot be transported independently across the plant biofilm system and needs the assistance of corresponding sugar transporters. SWEET(sugars will eventually be exported transporters)is a class of sugar transporters,it can carry out bidirectional transport of sugar in plants,and participate in many important physiological processes of plant growth and development by regulating the transport,distribution and storage of sugar compounds in plants. In this study,we cloned a gene named MdSWEET1. Expression analysis suggested that MdSWEET1 expressed mainly in stems and flowers and can respond to various abiotic stresses including NaCl、PEG、H_2 O_2 and ABA. Ectopic expressing MdSWEET1 in tomato enhanced salt tolerance and accumulated more soluble sugar especially sucrose and fructose.
Sugars are important energy source and signaling molecule in plant,and play important roles in plant growth and development as well as biotic and abiotic stresses. Sugar cannot be transported independently across the plant biofilm system and needs the assistance of corresponding sugar transporters. SWEET(sugars will eventually be exported transporters)is a class of sugar transporters,it can carry out bidirectional transport of sugar in plants,and participate in many important physiological processes of plant growth and development by regulating the transport,distribution and storage of sugar compounds in plants. In this study,we cloned a gene named MdSWEET1. Expression analysis suggested that MdSWEET1 expressed mainly in stems and flowers and can respond to various abiotic stresses including NaCl、PEG、H_2 O_2 and ABA. Ectopic expressing MdSWEET1 in tomato enhanced salt tolerance and accumulated more soluble sugar especially sucrose and fructose.
引文
Baker R F,Leach K A,Braun D M.2012.SWEET as sugar:new sucrose effluxers in plants.Molecular Plant,5:766-768.
Blum A,Mayer J,Gozlan G.1983.Association between plant production and some physiolocal components of drought resistance in wheat.Plant Cell&Environment,6:219-225.
Chardon F,Bedu M,Calenge F,Klemens P A,Spinner L,Clement G,Chietera G,Leran S,Ferrand M,Lacombe B,Loudet O,Dinant S,Bellini C,Neuhuas H E,Daniel V F,Krapp A.2013.Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis.Current Biology,23:697-702.
Chen L Q.2012.Sucrose efflux mediated by SWEET proteins as a key step for phloem transport.Science,335:207-211.
Chen L Q,Hou B H,Lalonde S.2010.Sugar transporters for intercellular exchange and nutrition of pathogens.Nature,468:527-532.
Chen L Q,Lin I W,Qu X Q,Sosso D,Mcfarlane H E,Londo?o A,Samuels A L,Frommer W B.2015.A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo.The Plant Cell,27:607.
Gill P K,Sharma A D,Singh P,Bhullar S S.2003.Changes in germination,growth and soluble sugar contents of Sorghum bicolor(L.)Moench seeds under various abiotic stresses.Plant Growth Regulation,40:157-162.
Hajlaoui H,Ayeb N E,Garrec J P,Denden M.2010.Differential effects of salt stress on osmotic adjustment and solutes allocation on the basis of root and leaf tissue senescence of two silage maize(Zea mays L.)varieties.Industrial Crops and Products,31:122-130.
Hir R L,Spinner L,Klemens P A W,Chakraborti D,Marco F D,Vilaine F O,Wolff N,Lemoine R,Porcheron B,Gery C,Teoule E,Chabout S,Mouille G,Neuhaus H E,Dinant S,Bellini C.2015.Disruption of the sugar transporters At SWEET11 and At SWEET12 affects vascular development and freezing tolerance in Arabidopsis.Molecular Plant,8:1687-1690.
Kidokoro S,Watanabe K,Ohori T,Moriwaki T,Maruyama K,Mizoi J,Myint P,Fujita Y,Sekita S,Shinozaki K.2015.Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression.The Plant Journal,81:505-518.
Klemens P A W,Patzke K,Deitmer J,Spinner L,Hir R L,Catherine B C,Bedu M,Chardon F,Krapp A,Neuhaus H E.2013.Overexpression of the vacuolar sugar carrier At SWEET16 modifies germination,growth,and stress tolerance in Arabidopsis.Plant Physiology,163:1338-1352.
Krasensky J,Jonak C.2012.Drought,salt,and temperature stress-induced metabolic rearrangements and regulatory networks.Journal of Experimental Botany,63:1593-1608.
Liu X Z,Zhang Y,Yang C,Tian Z H,Li J X.2016.At SWEET4,a hexose facilitator,mediates sugar transport to axial sinks and affects plant development.Scientific Reports,6:24563.
Lu J,Liu X,Ma Q J,Kang H,Liu Y J,Hao Y J,You C X.2019.Molecular cloning and functional characterization of the Aluminum activated malate transporter gene Md ALMT14.Scientia Horticulturae,244:208-217.
Nayer M,Reza H.2008.Drought-induced accumulation of soluble sugars and proline in two maize varieties.WASJ,3:448-453.
Ohto M A,Onai K,Furukawa Y,Aoki E,Araki T,Nakamura K.2001.Effects of sugar on vegetative development and floral transition in Arabidopsis.Plant Physiology,127:252-261.
Pattanagul W,Thitisaksakul M.2008.Effect of salinity stress on growth and carbohydrate metabolism in three rice(Oryza sativa L.)cultivars differing in salinity tolerance.Journal of Experimental Biology,46:10.
Prado F E,Boero C,Gallardo M,González J A.2000.Effect of NaC l on germination,growth,and soluble sugar content in Chenopodium quinoa Willd.Seeds.Botanical Bulletin of Academia Sinica,41(1):27-34.
Qi Chun-fang,Wang Ting,Liu Xia-yu,Shi Xiao-wen,Yu Zhi-qin,Liu Shi-fang,Liang Jian-ping,Jia Xiao-yun.2018.Study on improvement of tomato genetic transformation and screening and identification.Acta Agricultrae Boreali-occidentalis Sinica,27:554-561.(in Chinese)祁春芳,王婷,刘霞宇,石晓雯,于治芹,刘世芳,梁建萍,贾小云.2018.番茄遗传转化与筛选鉴定的改进研究.西北农业学报,27:554-561.
Rae A,Perroux J M,Grof C P L.2005.Sucrose partitioning between vascular bundles and storage parenchyma in the sugarcane stem:a potential role for the Sh SUT1 sucrose transporter.Planta,220(6):817-825.
Rahnama H,Ebrahimzadeh H.2004.The effect of Na Cl on proline accumulation in potato seedlings and calli.Acta Physiologiae Plantarum,26:263-270.
Ranganayakulu G S,Veeranagamallaiah G.Sudhakar C.2013.Effect of salt stress on osmolyte accumulation in two groundnut cultivars(Arachis hypogaea L.)with contrasting salt tolerance.African Journal of Plant Science,7:586-592.
Rolland F,Baena-Gonzalez E,Sheen J.2006.Sugar sensing and signaling in plants:conserved and novel mechanisms.Annual Review of Plant Biology.57(1):675-709.
Rosa M,Hilal M,González J A,Prado F E.2004.Changes in soluble carbohydrates and related enzymes induced by low temperature during early developmental stages of quinoa(Chenopodium quinoa)seedlings.Journal of Plant Physiology,161:683-689.
Shabala S.2013.Learning from halophytes:physiological basis and strategies to improve abiotic stress tolerance in crops.Ann Bot-London,112:1209-1221.
Singh M,Kumar J,Singh S,Singh V P,Prasad S M.2015.Roles of osmoprotectants in improving salinity and drought tolerance in plants:a review.Reviews Environmental Science and Biology,14:407-426.
Siringam K,Juntawong N,Cha-um S,Kirdmanee C.2011.Salt stress induced ion accumulation,ion homeostasis,membrane injury and sugar contents in salt-sensitive rice(Oryza sativa L.spp.indica)roots under osmotic conditions.African Journal of Biotechnology,10:1340-1346.
Sun M X,Huang X Y,Yang J,Guan Y F,Yang Z N.2013.Arabidopsis RPG1 is important for primexine deposition and functions redundantly with RPG2 for plant fertility at the late reproductive stage.Plant Reproduction,26:83-91.
Talbot N J.2010.Raiding the sweet shop.Nature,468:527-532.
Wang Yong-zhang,Zhang Da-peng.2000.Regulating effects of ethylene on carbohydrate metabolism in apple fruit during the ripening period.Acta Horticulturae Sinica,27:391-395.(in Chinese)王永章,张大鹏.2000.乙烯对成熟期新红星苹果果实碳水化合物代谢的调控.园艺学报,27:391-395.
Wang Hai-bo,Chen Xue-sen,Xin Pei-gang,Zhang Xiao-yan,Ci Zhi-juan,Shi Jun,Zhang Hong.2007.Study on sugar and acid constituents in several early apple cultivars and evaluation of their flavor quality.Journal of Fruit Science,24(4):513-516.(in Chinese)王海波,陈学森,辛培刚,张晓燕,慈志娟,石俊,张红.2007.几个早熟果品种果实糖酸组分及风味品质的评价.果树学报,24(4):513-516.
Zhao Shi-jie,Xu Chang-cheng,Zou Qi,Meng Qing-wei.1994.Improvements of method for measurement of malondialdehyde in plant tissues.Plant Physiol Commun,30:207-210.(in Chinese)赵世杰,许长成,邹琦,孟庆伟.1994.植物组织中丙二醛测定方法的改进.植物生理学通讯,30:207-210.
Zhang X X,Shi Z Q,Tian Y J,Zhou Q,Cai J,Dai T B,Cao W X,Pu H C,Jiang D.2016.Salt stress increases content and size of glutenin macropolymers in wheat grain.Food Chemistry,197:516-521.
Zhao X Y,Bian X Y,Li Z X,Wang X W,Yang C J,Liu G F,Jiang J,Kentbayev Y,Kentbayeva B,Yang C P.2014.Genetic stability analysis of introduced Betula pendula,Betula kirghisorum,and Betula pubescens families in saline-alkali soil of northeastern China.Scandinavian Journal of Forest Research,29:639-649.
Zhou A,Ma H,Feng S,Gong S,Wang J G.2018a.A novel sugar transporter from Dianthus spiculifolius,Ds SWEET12,affects sugar metabolism and confers osmotic and oxidative stress tolerance in Arabidopsis.International Journal of Molecular Siences,19(2):497.
Zhou A,Ma H,Feng S,Gong S,Wang J G.2018b.Ds SWEET17,a tonoplast-localized sugar transporter from Dianthus spiculifolius,affects sugar metabolism and confers multiple stress tolerance in Arabidopsis.International Journal of Molecular Siences,19(6):1564.
Blum A,Mayer J,Gozlan G.1983.Association between plant production and some physiolocal components of drought resistance in wheat.Plant Cell&Environment,6:219-225.
Chardon F,Bedu M,Calenge F,Klemens P A,Spinner L,Clement G,Chietera G,Leran S,Ferrand M,Lacombe B,Loudet O,Dinant S,Bellini C,Neuhuas H E,Daniel V F,Krapp A.2013.Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis.Current Biology,23:697-702.
Chen L Q.2012.Sucrose efflux mediated by SWEET proteins as a key step for phloem transport.Science,335:207-211.
Chen L Q,Hou B H,Lalonde S.2010.Sugar transporters for intercellular exchange and nutrition of pathogens.Nature,468:527-532.
Chen L Q,Lin I W,Qu X Q,Sosso D,Mcfarlane H E,Londo?o A,Samuels A L,Frommer W B.2015.A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo.The Plant Cell,27:607.
Gill P K,Sharma A D,Singh P,Bhullar S S.2003.Changes in germination,growth and soluble sugar contents of Sorghum bicolor(L.)Moench seeds under various abiotic stresses.Plant Growth Regulation,40:157-162.
Hajlaoui H,Ayeb N E,Garrec J P,Denden M.2010.Differential effects of salt stress on osmotic adjustment and solutes allocation on the basis of root and leaf tissue senescence of two silage maize(Zea mays L.)varieties.Industrial Crops and Products,31:122-130.
Hir R L,Spinner L,Klemens P A W,Chakraborti D,Marco F D,Vilaine F O,Wolff N,Lemoine R,Porcheron B,Gery C,Teoule E,Chabout S,Mouille G,Neuhaus H E,Dinant S,Bellini C.2015.Disruption of the sugar transporters At SWEET11 and At SWEET12 affects vascular development and freezing tolerance in Arabidopsis.Molecular Plant,8:1687-1690.
Kidokoro S,Watanabe K,Ohori T,Moriwaki T,Maruyama K,Mizoi J,Myint P,Fujita Y,Sekita S,Shinozaki K.2015.Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression.The Plant Journal,81:505-518.
Klemens P A W,Patzke K,Deitmer J,Spinner L,Hir R L,Catherine B C,Bedu M,Chardon F,Krapp A,Neuhaus H E.2013.Overexpression of the vacuolar sugar carrier At SWEET16 modifies germination,growth,and stress tolerance in Arabidopsis.Plant Physiology,163:1338-1352.
Krasensky J,Jonak C.2012.Drought,salt,and temperature stress-induced metabolic rearrangements and regulatory networks.Journal of Experimental Botany,63:1593-1608.
Liu X Z,Zhang Y,Yang C,Tian Z H,Li J X.2016.At SWEET4,a hexose facilitator,mediates sugar transport to axial sinks and affects plant development.Scientific Reports,6:24563.
Lu J,Liu X,Ma Q J,Kang H,Liu Y J,Hao Y J,You C X.2019.Molecular cloning and functional characterization of the Aluminum activated malate transporter gene Md ALMT14.Scientia Horticulturae,244:208-217.
Nayer M,Reza H.2008.Drought-induced accumulation of soluble sugars and proline in two maize varieties.WASJ,3:448-453.
Ohto M A,Onai K,Furukawa Y,Aoki E,Araki T,Nakamura K.2001.Effects of sugar on vegetative development and floral transition in Arabidopsis.Plant Physiology,127:252-261.
Pattanagul W,Thitisaksakul M.2008.Effect of salinity stress on growth and carbohydrate metabolism in three rice(Oryza sativa L.)cultivars differing in salinity tolerance.Journal of Experimental Biology,46:10.
Prado F E,Boero C,Gallardo M,González J A.2000.Effect of NaC l on germination,growth,and soluble sugar content in Chenopodium quinoa Willd.Seeds.Botanical Bulletin of Academia Sinica,41(1):27-34.
Qi Chun-fang,Wang Ting,Liu Xia-yu,Shi Xiao-wen,Yu Zhi-qin,Liu Shi-fang,Liang Jian-ping,Jia Xiao-yun.2018.Study on improvement of tomato genetic transformation and screening and identification.Acta Agricultrae Boreali-occidentalis Sinica,27:554-561.(in Chinese)祁春芳,王婷,刘霞宇,石晓雯,于治芹,刘世芳,梁建萍,贾小云.2018.番茄遗传转化与筛选鉴定的改进研究.西北农业学报,27:554-561.
Rae A,Perroux J M,Grof C P L.2005.Sucrose partitioning between vascular bundles and storage parenchyma in the sugarcane stem:a potential role for the Sh SUT1 sucrose transporter.Planta,220(6):817-825.
Rahnama H,Ebrahimzadeh H.2004.The effect of Na Cl on proline accumulation in potato seedlings and calli.Acta Physiologiae Plantarum,26:263-270.
Ranganayakulu G S,Veeranagamallaiah G.Sudhakar C.2013.Effect of salt stress on osmolyte accumulation in two groundnut cultivars(Arachis hypogaea L.)with contrasting salt tolerance.African Journal of Plant Science,7:586-592.
Rolland F,Baena-Gonzalez E,Sheen J.2006.Sugar sensing and signaling in plants:conserved and novel mechanisms.Annual Review of Plant Biology.57(1):675-709.
Rosa M,Hilal M,González J A,Prado F E.2004.Changes in soluble carbohydrates and related enzymes induced by low temperature during early developmental stages of quinoa(Chenopodium quinoa)seedlings.Journal of Plant Physiology,161:683-689.
Shabala S.2013.Learning from halophytes:physiological basis and strategies to improve abiotic stress tolerance in crops.Ann Bot-London,112:1209-1221.
Singh M,Kumar J,Singh S,Singh V P,Prasad S M.2015.Roles of osmoprotectants in improving salinity and drought tolerance in plants:a review.Reviews Environmental Science and Biology,14:407-426.
Siringam K,Juntawong N,Cha-um S,Kirdmanee C.2011.Salt stress induced ion accumulation,ion homeostasis,membrane injury and sugar contents in salt-sensitive rice(Oryza sativa L.spp.indica)roots under osmotic conditions.African Journal of Biotechnology,10:1340-1346.
Sun M X,Huang X Y,Yang J,Guan Y F,Yang Z N.2013.Arabidopsis RPG1 is important for primexine deposition and functions redundantly with RPG2 for plant fertility at the late reproductive stage.Plant Reproduction,26:83-91.
Talbot N J.2010.Raiding the sweet shop.Nature,468:527-532.
Wang Yong-zhang,Zhang Da-peng.2000.Regulating effects of ethylene on carbohydrate metabolism in apple fruit during the ripening period.Acta Horticulturae Sinica,27:391-395.(in Chinese)王永章,张大鹏.2000.乙烯对成熟期新红星苹果果实碳水化合物代谢的调控.园艺学报,27:391-395.
Wang Hai-bo,Chen Xue-sen,Xin Pei-gang,Zhang Xiao-yan,Ci Zhi-juan,Shi Jun,Zhang Hong.2007.Study on sugar and acid constituents in several early apple cultivars and evaluation of their flavor quality.Journal of Fruit Science,24(4):513-516.(in Chinese)王海波,陈学森,辛培刚,张晓燕,慈志娟,石俊,张红.2007.几个早熟果品种果实糖酸组分及风味品质的评价.果树学报,24(4):513-516.
Zhao Shi-jie,Xu Chang-cheng,Zou Qi,Meng Qing-wei.1994.Improvements of method for measurement of malondialdehyde in plant tissues.Plant Physiol Commun,30:207-210.(in Chinese)赵世杰,许长成,邹琦,孟庆伟.1994.植物组织中丙二醛测定方法的改进.植物生理学通讯,30:207-210.
Zhang X X,Shi Z Q,Tian Y J,Zhou Q,Cai J,Dai T B,Cao W X,Pu H C,Jiang D.2016.Salt stress increases content and size of glutenin macropolymers in wheat grain.Food Chemistry,197:516-521.
Zhao X Y,Bian X Y,Li Z X,Wang X W,Yang C J,Liu G F,Jiang J,Kentbayev Y,Kentbayeva B,Yang C P.2014.Genetic stability analysis of introduced Betula pendula,Betula kirghisorum,and Betula pubescens families in saline-alkali soil of northeastern China.Scandinavian Journal of Forest Research,29:639-649.
Zhou A,Ma H,Feng S,Gong S,Wang J G.2018a.A novel sugar transporter from Dianthus spiculifolius,Ds SWEET12,affects sugar metabolism and confers osmotic and oxidative stress tolerance in Arabidopsis.International Journal of Molecular Siences,19(2):497.
Zhou A,Ma H,Feng S,Gong S,Wang J G.2018b.Ds SWEET17,a tonoplast-localized sugar transporter from Dianthus spiculifolius,affects sugar metabolism and confers multiple stress tolerance in Arabidopsis.International Journal of Molecular Siences,19(6):1564.