峨眉拟单性木兰B类基因的克隆及系统进化分析
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摘要
MADS-box基因是一类在花发育进程中起重要作用的基因。本研究通过同源克隆技术从峨眉拟单性木兰花芽中分离得到3个B-class MADS-Box基因的CDS全长,分别命名为PaomAP3、PaomPI1、PaomPI2,并对其进行生物信息学分析。结果表明,PaomAP3基因ORF(开放阅读框)长度为642 bp,编码214个氨基酸;PaomPI1基因ORF长度为633 bp,编码210个氨基酸;PaomPI2基因ORF长度为636 bp,编码211个氨基酸。氨基酸序列分析结果表明,三个基因均含有典型的MADS结构域及K结构域。PaomPI1、PaomPI2基因末端有典型的PI-derived保守基元,PaomAP3基因末端有Paleo AP3保守基元和PI-derived保守基元。木兰科植物在科内分类上存在很大争议,因此本研究利用MEGA6软件中最大简约法(MP)构建了部分木兰科植物B类基因的系统发育树,结果表明玉兰亚属与木兰亚属亲缘关系较远;拟单性木兰属独成一属,其与含笑属、玉兰亚属亲缘关系较近,而与木兰亚属亲缘关系较远。
MADS-Box gene is a kind of vital gene which plays an important role in flower development.In this study,3 B-class MADS-Box genes of CDS full length were isolated and cloned from the flower buds of Parakmeria omeiensis by using homologous cloning technique,named as PaomAP3,PaomPI1,PaomPI2,respectively.Meanwhile,bioinformatics analysis was carried out.The results showed that PaomAP3 contained a complete ORF(open reading frame) of 642 bp that encoded 214 amino acids;PaomPI1 contained a complete ORF of 633 bp that encoded 210 amino acids;PaomPI2 contained a complete ORF of 636 bp that encoded 211 amino acids.The results of amino acid sequence analysis showed that these 3 genes contained typical MADS domain and K domain.The PaomPI1 and PaomPI2 genes both had typical PI-derived conserved motifs,and the PaomAP3 gene ended with Paleo AP3 conserved motifs and PI-derived conserved motifs.There is a great deal of controversy about the classification of Magnoliaceae.Therefore,the phylogenetic tree of B-class genes in some Magnoliaceae plants was constructed by the maximum parsimony meth od(MP) in MEGA6 software.The results showed that the subgenus Yulania was genetically distinct from the subgenus Magnolia.The Parakmeria was a solitary genus with close relationship with Michelia L.and Subgenus Yulania,but far from Magnolia subgenus.
MADS-Box gene is a kind of vital gene which plays an important role in flower development.In this study,3 B-class MADS-Box genes of CDS full length were isolated and cloned from the flower buds of Parakmeria omeiensis by using homologous cloning technique,named as PaomAP3,PaomPI1,PaomPI2,respectively.Meanwhile,bioinformatics analysis was carried out.The results showed that PaomAP3 contained a complete ORF(open reading frame) of 642 bp that encoded 214 amino acids;PaomPI1 contained a complete ORF of 633 bp that encoded 210 amino acids;PaomPI2 contained a complete ORF of 636 bp that encoded 211 amino acids.The results of amino acid sequence analysis showed that these 3 genes contained typical MADS domain and K domain.The PaomPI1 and PaomPI2 genes both had typical PI-derived conserved motifs,and the PaomAP3 gene ended with Paleo AP3 conserved motifs and PI-derived conserved motifs.There is a great deal of controversy about the classification of Magnoliaceae.Therefore,the phylogenetic tree of B-class genes in some Magnoliaceae plants was constructed by the maximum parsimony meth od(MP) in MEGA6 software.The results showed that the subgenus Yulania was genetically distinct from the subgenus Magnolia.The Parakmeria was a solitary genus with close relationship with Michelia L.and Subgenus Yulania,but far from Magnolia subgenus.
引文
Chen B.L.,a nd Nooteboom H.P.,1993,Notes on magnoliaceaeⅢ:The magnoliaceae of China,Annals of the Missouri Botanical Garden,80(4):999-1104
Cheng S.,Bergh E.V.D.,and Zeng P.,2013,The tarenaya hassleriana genome provides insight into reproductive trait and genome evolution of crucifers,Plant Cell,25(8):2813-2830
Coen E.S.,and Meyerowitz E.M.,1991,The war of the whorls:genetic interactions controlling flower development,Nature,353:31-37
Colombo L.,Franken J.,Koetje E.,Went J.V.,Dons H.J.,Tunen A.J.,and Angenent G.C.,1995,The Petunia mads box gene FBP11 determines ovule identity,Plant Cell,7(11):1859-1868
Dandy J.E.,1927,The genera of manoliaceae,Kew Bulletin,7:264-275
Dandy J.E.,1964,Magnoliaceae,In:Hutchinson J.(ed.),The genera of flowering plants,Clarendron Press,Oxford,US,pp.50-57
Gong X.,Pan Y.Z.,and Yang Z.Y.,2001,The cross-compatibility of magnoliaceae,Zhiwu Fenlei Yu Ziyuan Xuebao(Acta Botanica Yunnanica),26(3):339-344(龚洵,潘跃芝,杨志云,2001,木兰科植物的杂交亲和性,植物分类与资源学报,26(3):339-344)
Guo C.C.,2012,Studies on the history,patterns and mechanisms of MADS-box gene evolution-concurrently on the conservation of orthologs,Thesis for M.S.,Graduate School of Chinese Academy of Sciences,Supervisor:Kong H.Z.,pp.8-10(国春策,2012,MADS-box基因进化的历史、式样和机制研究-兼论直系同源基因的保守性问题,硕士学位论文,中国科学院研究生院,导师:孔宏智,pp.8-10)
Honma T.,and Goto K.,2001,Complexes of mads-box proteins are sufficient to convert leaves into floral organs,Nature,409(6819):525-529
Hu X.S.,and Zheng W.J.,1951,Parakmeria,a new genus of Magnoliaceae of southwestern China,Zhongguo Kexueyuan Daxue Xuebao(Journal of University of Chinese Academy of Sciences),1(1):1-3(胡先骕,郑万钧,1951,拟克枺丽木,中国西南部木兰科一新属,中国科学院大学学报,1(1):1-3)Kim S.,Albert V.A.,Farris J.S.,Soltis P.S.,and Soltis D.E.,2004,Phylogeny and diversification of B-function mads-box genes in angiosperms:evolutionary and functional implications of a 260-million-year-old duplication,Am.J.Bot.,91(12):2102-2118Kim S.,Park C.W.,Kim Y.D.,and Suh Y.,2001,Phylogenetic relationships in family Magnoliaceae inferred from ndh F sequences,Am.J.Bot.,88(4):717-728Kramer E.M.,Dorit R.L.,and Irish V.F.,1998,Molecular evolution of genes controlling petal and stamen development:duplication and divergence within the APETALA3 and PISTI-LLATA MADS-box gene lineages,Genetics,149(2):765-783Krizek B.A.,and Fletcher J.C.,2005,Molecular mechanisms of flower development:an armchair guide,Nat.Rev.Genet.,6(9):688-698Krizek B.A.,and Meyerowitz E.M.,1996,The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the b class organ identity function,Development,122(1):11-22Li Y.Y.,Sima Y.K.,Fang B.,Guo L.Q.,Jiang H.,and Zhao W.S.,2003,Current situation and evaluation of natural resources of the priority protection wild plants in Yunnan province of China,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),25(2):181-191(李玉媛,司马永康,方波,郭立群,蒋宏,赵文书,2003,云南省国家重点保护野生植物资源的现状与评价,植物分类与资源学报,25(2):181-191)Lin Q.,Duan L.D.,Yuan Q.,Li M.H.,and Xie Y.H.,2006,Taxonomic revision of the genus Parakmeria Hu&Cheng(Magnoliaceae),Zhiwu Yanjiu(Bulletin of Botanical Research),26(5):527-531(林祁,段林东,袁琼,李明红,谢永红,2006,拟单性木兰属(木兰科)植物的分类学修订,植物研究,26(5):527-531)
Liu Y.H.,1984,A preliminary study on the taxonomy of the family magnoliaceae,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),22(2):88-107(刘玉壶,1984,木兰科分类系统的初步研究,植物分类学报,22(2):88-107)
Liu Y.H.,Xia N.H.,and Yang H.Q.,1995,The origin,evolution and phytogeography of magnoliaceae,Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropicak),3(4):1-12(刘玉壶,夏念和,杨惠秋,1995,木兰科的起源、进化和地理分布,热带亚热带植物学报,3(4):1-12)
Ma G.Y.,Liu H.C.,Zou Q.C.,Zhu K.Y.,Zhou J.Y.,Zhan J.,and Tian D.Q.,2014,Cloning and expression analysis of b class gene Aa PI from Anthurium andraeanum,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(5):950-955(马广莹,刘慧春,邹清成,朱开元,周江华,詹菁,田丹青,2014,红掌花发育b类基因Aa PI的克隆及表达分析,分子植物育种,12(5):950-955)
Mcgonigle B.,Bouhidel K.,and Irish V.F.,1996,Nuclear localization of the Arabidopsis APETALA3 and PISTILLATAhomeotic gene products depends on their simultaneous expression,Genes Dev.,10(14):1812-1821
Messenguy F.,and Dubois E.,2003,Role of MADS box proteins and their cofactors in combinatorial control of gene expression and cell development,Gene,316(1):1-21
Nie Z.L.,Wen J.,Azuma H.,Qiu Y.L.,Sun H.,Meng Y.,Sun W.B.,and Zimmer E.A.,2008,Phylogenetic and biogeographic complexity of Magnoliaceae in the northern hemisphere inferred from three nuclear data set,Mol.Phylogenet Evol.,48(3):1027-1040
Nooteboom H.P.,1985,Notes on magnoliaceae with a revision of pachylarnax and elmerrillia and the malesian species of manglietia and michelia,Blumea-Biodiversity,Evolution and Biogeography of Plants,31(1):65-121
Pan H.C.,Shi S.H.,and Jin H.,1999,Phylogeny of Parakmeria(Magnoliaceae)and its related taxa inferred from the mat Kgene sequence,Zhongshan Daxue Xuebao(Acta Scientiarum Naturalium Universitatis Sunyatseni(Natural Science Edition)),38(3):63-67(潘恒昶,施苏华,金虹,1999,拟单性木兰属近缘植物mat K基因的序列分析,中山大学学报(自然科学版),38(3):63-67)
Riechmann J.L.,Wang M.,and Meyerowitz E.M.,1996,Dnabinding properties of Arabidopsis mads domain homeotic proteins APETALA1,APETALA3,PISTILLATA and AG-AMOUS,Nucleic Acids Res.,24(16):3134-3141
Rounsley S.D.,Ditta G.S.,and Yanofsky M.F.,1995,Diverse roles for mads box genes in Arabidopsis development,Plant Cell,7(8):1259-1269
Shi S.,Jin H.,Zhong Y.,He X.,Huang Y.,Tan F.,and Boufford D.E.,2000,Phylogenetic relationships of the magnoliaceae inferred from cpdna mat K sequences,Theor.Appl.Genet.,101(5):925-930
Sima Y.K.,Lu S.G.,Han M.Y.,Ma H.F.,Xu T.,Xu L.,Hao J.B.,Li D.,and Chen S.Y.,2012,Advances in Magnoliaceae systematic research,Xibu Linye Kexue(Journal of West China Forestry Science),41(1):116-127(司马永康,陆树刚,韩明跃,马慧芬,徐涛,徐亮,郝佳波,李丹,陈少瑜,2012,木兰科系统分类学研究动态,西部林业科学,41(1):116-127)
Stellari G.M.,Jaramillo M.A.,and Kramer E.M.,2004,Evolution of the APETALA3 and PISTILLATA lineages of mads-boxcontaining genes in the basal angiosperms,Mol.Biol.Evol.,21(3):506-519
Theissen G.,2001,Development of floral organ identity:stories from the MADS house,Curr.Opin.Plant Biol.,4(1):75-85
Vandenbussche M.,Theissen G.,Vande Peer Y.,and Gerats T.,2003,Structural diversification and neo-functionalization during floral mads-box gene evolution by C-terminal frameshift mutations,Nucleic Acids Res.,31(15):4401-4409
Wang Y.L.,Li Y.,Zhang S.Z.,and Yu X.S.,2006,The unility of mat K gene in the phylogenetic analysis of the genus Magnolia,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),23(2):211-223(王亚玲,李勇,张寿洲,余兴生,2006,用mat K序列分析探讨木兰属植物的系统发育关系,植物分类学报,23(2):211-223)
Xu F.X.,2002,Study on pollen morphology of Parakmeria lotungensis,Guangxi Zhiwu(Guihaia),22(2):157-159(徐凤霞,2002,乐东拟单性木兰花粉形态观察,广西植物,22(2):157-159)
Xu F.X.,and Wu Q.G.,2002,Chalazal region morphology on the endotesta of Magnoliaceous seeds and its systematic significance,Zhiwu Fenlei Xuebao(Acta Phytotaxonomica Sinica),40(3):260-270(徐凤霞,吴七根,2002,木兰科种子内种皮合点区形态及其系统学意义,植物分类学报,40(3):260-270)
Xu F.X.,Chen Z.Y.,and Zhang D.X.,2000,A cladistic analysis of Magnoliaceae,Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),8(3):207-214(徐凤霞,陈忠毅,张奠湘,2000,木兰科的分支分析,热带亚热带植物学报,8(3):207-214)
Yang Y.,and Jack T.,2004,Defining subdomains of the k domain important for protein-protein interactions of plant mads proteins,Plant Mol.Biol.,55(1):45-59
Zeng Y.,Hu J.Y.,and Li Z.J.,2001,MADS-Box genes in the evolution of flower development,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),37(4):281-287(曾英,胡金勇,李志坚,2001,植物MADS盒基因与花器官的进化发育,植物生理学通讯,37(4):281-287)
Zhao J.H.,Jia Q.,Jin H.T.,and Sheng Y.M.,2014,Evolution analysis of the chloroplast genes atp B and rbc L of Magnolia,Changchun Shifan Daxue Xuebao(Journal of Changchun Normal University),(12):71-74(赵晶华,贾倩,金洪涛,盛彦敏,2014,木兰叶绿体atp B和rbc L基因的系统进化分析,长春师范大学学报,(12):71-74)
Cheng S.,Bergh E.V.D.,and Zeng P.,2013,The tarenaya hassleriana genome provides insight into reproductive trait and genome evolution of crucifers,Plant Cell,25(8):2813-2830
Coen E.S.,and Meyerowitz E.M.,1991,The war of the whorls:genetic interactions controlling flower development,Nature,353:31-37
Colombo L.,Franken J.,Koetje E.,Went J.V.,Dons H.J.,Tunen A.J.,and Angenent G.C.,1995,The Petunia mads box gene FBP11 determines ovule identity,Plant Cell,7(11):1859-1868
Dandy J.E.,1927,The genera of manoliaceae,Kew Bulletin,7:264-275
Dandy J.E.,1964,Magnoliaceae,In:Hutchinson J.(ed.),The genera of flowering plants,Clarendron Press,Oxford,US,pp.50-57
Gong X.,Pan Y.Z.,and Yang Z.Y.,2001,The cross-compatibility of magnoliaceae,Zhiwu Fenlei Yu Ziyuan Xuebao(Acta Botanica Yunnanica),26(3):339-344(龚洵,潘跃芝,杨志云,2001,木兰科植物的杂交亲和性,植物分类与资源学报,26(3):339-344)
Guo C.C.,2012,Studies on the history,patterns and mechanisms of MADS-box gene evolution-concurrently on the conservation of orthologs,Thesis for M.S.,Graduate School of Chinese Academy of Sciences,Supervisor:Kong H.Z.,pp.8-10(国春策,2012,MADS-box基因进化的历史、式样和机制研究-兼论直系同源基因的保守性问题,硕士学位论文,中国科学院研究生院,导师:孔宏智,pp.8-10)
Honma T.,and Goto K.,2001,Complexes of mads-box proteins are sufficient to convert leaves into floral organs,Nature,409(6819):525-529
Hu X.S.,and Zheng W.J.,1951,Parakmeria,a new genus of Magnoliaceae of southwestern China,Zhongguo Kexueyuan Daxue Xuebao(Journal of University of Chinese Academy of Sciences),1(1):1-3(胡先骕,郑万钧,1951,拟克枺丽木,中国西南部木兰科一新属,中国科学院大学学报,1(1):1-3)Kim S.,Albert V.A.,Farris J.S.,Soltis P.S.,and Soltis D.E.,2004,Phylogeny and diversification of B-function mads-box genes in angiosperms:evolutionary and functional implications of a 260-million-year-old duplication,Am.J.Bot.,91(12):2102-2118Kim S.,Park C.W.,Kim Y.D.,and Suh Y.,2001,Phylogenetic relationships in family Magnoliaceae inferred from ndh F sequences,Am.J.Bot.,88(4):717-728Kramer E.M.,Dorit R.L.,and Irish V.F.,1998,Molecular evolution of genes controlling petal and stamen development:duplication and divergence within the APETALA3 and PISTI-LLATA MADS-box gene lineages,Genetics,149(2):765-783Krizek B.A.,and Fletcher J.C.,2005,Molecular mechanisms of flower development:an armchair guide,Nat.Rev.Genet.,6(9):688-698Krizek B.A.,and Meyerowitz E.M.,1996,The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the b class organ identity function,Development,122(1):11-22Li Y.Y.,Sima Y.K.,Fang B.,Guo L.Q.,Jiang H.,and Zhao W.S.,2003,Current situation and evaluation of natural resources of the priority protection wild plants in Yunnan province of China,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),25(2):181-191(李玉媛,司马永康,方波,郭立群,蒋宏,赵文书,2003,云南省国家重点保护野生植物资源的现状与评价,植物分类与资源学报,25(2):181-191)Lin Q.,Duan L.D.,Yuan Q.,Li M.H.,and Xie Y.H.,2006,Taxonomic revision of the genus Parakmeria Hu&Cheng(Magnoliaceae),Zhiwu Yanjiu(Bulletin of Botanical Research),26(5):527-531(林祁,段林东,袁琼,李明红,谢永红,2006,拟单性木兰属(木兰科)植物的分类学修订,植物研究,26(5):527-531)
Liu Y.H.,1984,A preliminary study on the taxonomy of the family magnoliaceae,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),22(2):88-107(刘玉壶,1984,木兰科分类系统的初步研究,植物分类学报,22(2):88-107)
Liu Y.H.,Xia N.H.,and Yang H.Q.,1995,The origin,evolution and phytogeography of magnoliaceae,Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropicak),3(4):1-12(刘玉壶,夏念和,杨惠秋,1995,木兰科的起源、进化和地理分布,热带亚热带植物学报,3(4):1-12)
Ma G.Y.,Liu H.C.,Zou Q.C.,Zhu K.Y.,Zhou J.Y.,Zhan J.,and Tian D.Q.,2014,Cloning and expression analysis of b class gene Aa PI from Anthurium andraeanum,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(5):950-955(马广莹,刘慧春,邹清成,朱开元,周江华,詹菁,田丹青,2014,红掌花发育b类基因Aa PI的克隆及表达分析,分子植物育种,12(5):950-955)
Mcgonigle B.,Bouhidel K.,and Irish V.F.,1996,Nuclear localization of the Arabidopsis APETALA3 and PISTILLATAhomeotic gene products depends on their simultaneous expression,Genes Dev.,10(14):1812-1821
Messenguy F.,and Dubois E.,2003,Role of MADS box proteins and their cofactors in combinatorial control of gene expression and cell development,Gene,316(1):1-21
Nie Z.L.,Wen J.,Azuma H.,Qiu Y.L.,Sun H.,Meng Y.,Sun W.B.,and Zimmer E.A.,2008,Phylogenetic and biogeographic complexity of Magnoliaceae in the northern hemisphere inferred from three nuclear data set,Mol.Phylogenet Evol.,48(3):1027-1040
Nooteboom H.P.,1985,Notes on magnoliaceae with a revision of pachylarnax and elmerrillia and the malesian species of manglietia and michelia,Blumea-Biodiversity,Evolution and Biogeography of Plants,31(1):65-121
Pan H.C.,Shi S.H.,and Jin H.,1999,Phylogeny of Parakmeria(Magnoliaceae)and its related taxa inferred from the mat Kgene sequence,Zhongshan Daxue Xuebao(Acta Scientiarum Naturalium Universitatis Sunyatseni(Natural Science Edition)),38(3):63-67(潘恒昶,施苏华,金虹,1999,拟单性木兰属近缘植物mat K基因的序列分析,中山大学学报(自然科学版),38(3):63-67)
Riechmann J.L.,Wang M.,and Meyerowitz E.M.,1996,Dnabinding properties of Arabidopsis mads domain homeotic proteins APETALA1,APETALA3,PISTILLATA and AG-AMOUS,Nucleic Acids Res.,24(16):3134-3141
Rounsley S.D.,Ditta G.S.,and Yanofsky M.F.,1995,Diverse roles for mads box genes in Arabidopsis development,Plant Cell,7(8):1259-1269
Shi S.,Jin H.,Zhong Y.,He X.,Huang Y.,Tan F.,and Boufford D.E.,2000,Phylogenetic relationships of the magnoliaceae inferred from cpdna mat K sequences,Theor.Appl.Genet.,101(5):925-930
Sima Y.K.,Lu S.G.,Han M.Y.,Ma H.F.,Xu T.,Xu L.,Hao J.B.,Li D.,and Chen S.Y.,2012,Advances in Magnoliaceae systematic research,Xibu Linye Kexue(Journal of West China Forestry Science),41(1):116-127(司马永康,陆树刚,韩明跃,马慧芬,徐涛,徐亮,郝佳波,李丹,陈少瑜,2012,木兰科系统分类学研究动态,西部林业科学,41(1):116-127)
Stellari G.M.,Jaramillo M.A.,and Kramer E.M.,2004,Evolution of the APETALA3 and PISTILLATA lineages of mads-boxcontaining genes in the basal angiosperms,Mol.Biol.Evol.,21(3):506-519
Theissen G.,2001,Development of floral organ identity:stories from the MADS house,Curr.Opin.Plant Biol.,4(1):75-85
Vandenbussche M.,Theissen G.,Vande Peer Y.,and Gerats T.,2003,Structural diversification and neo-functionalization during floral mads-box gene evolution by C-terminal frameshift mutations,Nucleic Acids Res.,31(15):4401-4409
Wang Y.L.,Li Y.,Zhang S.Z.,and Yu X.S.,2006,The unility of mat K gene in the phylogenetic analysis of the genus Magnolia,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),23(2):211-223(王亚玲,李勇,张寿洲,余兴生,2006,用mat K序列分析探讨木兰属植物的系统发育关系,植物分类学报,23(2):211-223)
Xu F.X.,2002,Study on pollen morphology of Parakmeria lotungensis,Guangxi Zhiwu(Guihaia),22(2):157-159(徐凤霞,2002,乐东拟单性木兰花粉形态观察,广西植物,22(2):157-159)
Xu F.X.,and Wu Q.G.,2002,Chalazal region morphology on the endotesta of Magnoliaceous seeds and its systematic significance,Zhiwu Fenlei Xuebao(Acta Phytotaxonomica Sinica),40(3):260-270(徐凤霞,吴七根,2002,木兰科种子内种皮合点区形态及其系统学意义,植物分类学报,40(3):260-270)
Xu F.X.,Chen Z.Y.,and Zhang D.X.,2000,A cladistic analysis of Magnoliaceae,Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),8(3):207-214(徐凤霞,陈忠毅,张奠湘,2000,木兰科的分支分析,热带亚热带植物学报,8(3):207-214)
Yang Y.,and Jack T.,2004,Defining subdomains of the k domain important for protein-protein interactions of plant mads proteins,Plant Mol.Biol.,55(1):45-59
Zeng Y.,Hu J.Y.,and Li Z.J.,2001,MADS-Box genes in the evolution of flower development,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),37(4):281-287(曾英,胡金勇,李志坚,2001,植物MADS盒基因与花器官的进化发育,植物生理学通讯,37(4):281-287)
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