陕西省5个主栽桑树品种桑叶主要活性成分的含量及活性比较
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摘要
为了解陕西省地理环境对桑叶主要活性成分的影响,利用液相色谱-质谱联用法对陕西省主栽的5个桑树品种桑叶中的5种主要活性成分进行定性及定量分析,同时通过DPPH、FRAP法和α-葡萄糖苷酶抑制率检测法测试其抗氧化性能和糖苷酶抑制活性。结果表明,不同桑品种桑叶中的活性成分含量及其生物活性均有显著差异。育711号中的荞麦碱和1-脱氧野尻霉素(DNJ)含量均显著高于其他品种,质量分数分别达到1.84和0.78 mg/g;桐乡青桑叶中的绿原酸和异槲皮苷含量显著高于其他品种,而强桑1号桑叶中的芦丁含量显著高于其他品种,质量分数分别为6.08、1.78和1.46 mg/g。桐乡青桑叶提取物的FRAP抗氧化活性显著高于其他品种,荷叶白桑叶提取物的DPPH自由基清除能力显著高于其他品种,经主成分分析发现这2个桑品种桑叶提取物的抗氧化活性优于其他品种。农桑14号桑叶提取物的α-葡萄糖苷酶抑制率显著高于其他品种,达到了95.90%。桑叶中的绿原酸含量普遍较高,质量分数达到0.39%～0.61%,表明桑叶可以作为一种富含绿原酸的潜在植物资源加以利用。
In order to understand the influence of geographical environment in Shaanxi Province on main active components in mulberry leaf,5 active components in mulberry leaf of five major mulberry varieties planted in Shaanxi were qualitatively and quantitatively analyzed by liquid chromatography-mass spectrometry( HPLC-MS/MS). Meanwhile,DPPH,FRAP and α-glucosidase inhibitory rate assay were used to determine the antioxidant activity and glycosidase inhibitory activity. Results showed that the contents of active components in mulberry leaf from different mulberry varieties and their bioactivity was significantly different. The contents of fagomine and 1-deoxynojirimycin( DNJ) in mulberry leaf of Yu 711 were significantly higher than those in other varieties,reaching to 1. 84 and 0. 78 mg/g,respectively.The contents of chlorogenic acid and isoquercitrin in mulberry leaf of Tongxiangqing and the content of rutin in mulberry leaf of Qiangsang 1 were significantly higher than those in other varieties,being 6. 08,1. 78 and 1. 46 mg/g,respectively. FRAP antioxidant activity of Tongxiangqing mulberry leaf extract was significantly higher than that of other mulberry varieties,the same as to the DPPH free radical scavenging ability of Heyebai mulberry leaf extract. Principal component analysis showed that the antioxidant activities of mulberry leaf extract of these two varieties were better than those of other varieties. The α-glucosidase inhibitory rate of mulberry leaf extract of Nongsang 14 was 95. 90%,significantly higher than that of other mulberry varieties. The content of chlorogenic acid in mulberry leaf was generally high,which reached to 0. 39%-0. 61%. The results indicate that mulberry leaf can be used as a potential plant resource rich in chlorogenic acid.
In order to understand the influence of geographical environment in Shaanxi Province on main active components in mulberry leaf,5 active components in mulberry leaf of five major mulberry varieties planted in Shaanxi were qualitatively and quantitatively analyzed by liquid chromatography-mass spectrometry( HPLC-MS/MS). Meanwhile,DPPH,FRAP and α-glucosidase inhibitory rate assay were used to determine the antioxidant activity and glycosidase inhibitory activity. Results showed that the contents of active components in mulberry leaf from different mulberry varieties and their bioactivity was significantly different. The contents of fagomine and 1-deoxynojirimycin( DNJ) in mulberry leaf of Yu 711 were significantly higher than those in other varieties,reaching to 1. 84 and 0. 78 mg/g,respectively.The contents of chlorogenic acid and isoquercitrin in mulberry leaf of Tongxiangqing and the content of rutin in mulberry leaf of Qiangsang 1 were significantly higher than those in other varieties,being 6. 08,1. 78 and 1. 46 mg/g,respectively. FRAP antioxidant activity of Tongxiangqing mulberry leaf extract was significantly higher than that of other mulberry varieties,the same as to the DPPH free radical scavenging ability of Heyebai mulberry leaf extract. Principal component analysis showed that the antioxidant activities of mulberry leaf extract of these two varieties were better than those of other varieties. The α-glucosidase inhibitory rate of mulberry leaf extract of Nongsang 14 was 95. 90%,significantly higher than that of other mulberry varieties. The content of chlorogenic acid in mulberry leaf was generally high,which reached to 0. 39%-0. 61%. The results indicate that mulberry leaf can be used as a potential plant resource rich in chlorogenic acid.
引文
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[14] YANG Z,WANG Y,WANG Y,et al.Bioassay-guided screening and isolation of α-glucosidase and tyrosinase inhibitors from leaves of Morus alba[J].Food Chem,2012,131(2):617-625
[15] 刘颖,郭明晔,白根本.绿原酸的研究进展[J].中药材,2012,35(7):1180-1185
[16] 刘放,陈冰冰,吴小平.HPLC法测定菊花中绿原酸的含量[J].西北药学杂志,2010,25(5):350-352
[17] 贺胜,周杏子,何海,等.桑叶生物碱类成分研究概况[J].中国实验方剂学杂志,2015,21(13):222-226
[18] 陈玲玲,刘炜,陈建国,等.桑叶黄酮对糖尿病小鼠调节血糖的作用机制研究[J].中国临床药理学杂志,2010,26(11):835-838
[19] 孟庆海,殷秋忆,郭静,等.4种不同桑叶提取物降血糖作用的筛选[J].中成药,2014,36(6):1288-1291
[2] 韩世玉.桑树资源概况及其多元化利用[J].贵州农业科学,2006,34(3):118-121
[3] HU X Q,JIANG L,ZHANG J G,et al.Quantitative determination of 1-deoxynojirimycin in mulberry leaves from 132 varieties[J].Ind Crops Prod,2013,49(8):782-789
[4] 符继红,张丽静,孙莲.离子色谱法测定不同产地桑叶中的无机阴离子和有机酸[J].内蒙古中医药,2006,25(2):26-28
[5] 李明聪,杨丹,郭英,等.桑叶中黄酮类化学成分及药理作用研究进展[J].辽宁中医杂志,2012,39(2):377-380
[6] ASANO N,OSEKI K,TOMIOKA E,et al.N-containing sugars from Morus alba and their glycosidase inhibitory activities[J].Carbohydr Res,1994,259(2):243-255
[7] ZHANG Y L,LUO J G,WAN C X,et al.Geranylated 2-arylbenzofurans from Morus alba var Tatarica.and their α-glucosidase and protein tyrosine phosphatase 1B inhibitory activities[J].Fitoterapia,2014,92:116-126
[8] 中国农业科学院蚕业研究所.中国桑树栽培学[M].上海:上海科学技术出版社,1985:45-49
[9] 陈松,刘宏程,储一宁,等.12个桑树品种桑叶中的1-脱氧野尻霉素含量测定与分析[J].蚕业科学,2007,33(4):637-641
[10] 吴晓敏,韩利文,王希敏,等.不同产地新鲜紫色马铃薯中花色苷及总酚的含量测定[J].中国食物与营养,2014,20(5):24-26
[11] 柴建新,万茵,付桂明,等.杜仲叶总黄酮含量测定方法优化[J].中国食品学报,2013,13(4):225-230
[12] 杨虎,张生堂,高国强.玫瑰黄酮的提取及其清除DPPH自由基活性研究[J].食品科学,2012,33(24):152-155
[13] ZHANG D,LUO M,WANG W,et al.Variation of active constituents and antioxidant activity in pyrola [P. incarnata Fich.]from different sites in Northeast China[J].Food Chem,2013,141(3):2213-2219
[14] YANG Z,WANG Y,WANG Y,et al.Bioassay-guided screening and isolation of α-glucosidase and tyrosinase inhibitors from leaves of Morus alba[J].Food Chem,2012,131(2):617-625
[15] 刘颖,郭明晔,白根本.绿原酸的研究进展[J].中药材,2012,35(7):1180-1185
[16] 刘放,陈冰冰,吴小平.HPLC法测定菊花中绿原酸的含量[J].西北药学杂志,2010,25(5):350-352
[17] 贺胜,周杏子,何海,等.桑叶生物碱类成分研究概况[J].中国实验方剂学杂志,2015,21(13):222-226
[18] 陈玲玲,刘炜,陈建国,等.桑叶黄酮对糖尿病小鼠调节血糖的作用机制研究[J].中国临床药理学杂志,2010,26(11):835-838
[19] 孟庆海,殷秋忆,郭静,等.4种不同桑叶提取物降血糖作用的筛选[J].中成药,2014,36(6):1288-1291