水杨酸对感染卷叶病毒‘蛇龙珠’葡萄光合作用和果实品质的影响
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摘要
为了提高感染卷叶病毒的酿酒葡萄品质,试验以感染卷叶病毒的‘蛇龙珠’葡萄为试材,研究叶面喷施不同浓度水杨酸对葡萄叶片光合作用、果实品质的影响。结果表明:水杨酸处理不能显著抑制葡萄叶片中卷叶病毒的侵染,但可以提高叶片叶绿素含量和净光合速率,提高果实中转化酶活性,促进糖分积累;葡萄谢花后20 mg/L水杨酸处理显著提高了叶片叶绿素含量和1,5-二磷酸核酮糖羧化/加氧酶(Rbuisco)活性,叶片净光合速率显著升高,同时也显著提高了果实中转化酶活性,提升果实品质。因此,葡萄谢花后适宜浓度的水杨酸处理可以通过提高叶片光合能力和果实中糖分积累相关酶活性来提高感染卷叶病毒葡萄的果实品质。
Taking Vitis vinifera L. ‘Carbernet Gernischet' which infected grapevine leaf-roll associated virus III as materials,the effects of foliar sprayed salicylic acid after anthesis on the photosynthesis and berry quality were studies to increase berry quality. The results showed that the virus content in leaf was not significantly affected by salicylic acid treatment,but the chlorophyll content,net photosynthesis rate were increased,the invertase activities and sugar content in berry were increased too. 20 mg/L salicylic acid treatment after anthesis significantly improved chlorophyll content,Rubisco activities and net photosynthetic rate,invertase activities and berry quality in berry were significantly increased too.From above results,it is concluded that foliar sprayed of suitable concentration salicylic acid after anthesis can improve berry quality of infected grapevine leaf-roll associated virus III by regulated photosynthetic efficiency and activities of sugar accumulation related enzymes.
Taking Vitis vinifera L. ‘Carbernet Gernischet' which infected grapevine leaf-roll associated virus III as materials,the effects of foliar sprayed salicylic acid after anthesis on the photosynthesis and berry quality were studies to increase berry quality. The results showed that the virus content in leaf was not significantly affected by salicylic acid treatment,but the chlorophyll content,net photosynthesis rate were increased,the invertase activities and sugar content in berry were increased too. 20 mg/L salicylic acid treatment after anthesis significantly improved chlorophyll content,Rubisco activities and net photosynthetic rate,invertase activities and berry quality in berry were significantly increased too.From above results,it is concluded that foliar sprayed of suitable concentration salicylic acid after anthesis can improve berry quality of infected grapevine leaf-roll associated virus III by regulated photosynthetic efficiency and activities of sugar accumulation related enzymes.
引文
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[2]Vega A,Gutierrez R A,Pena-Neira A,et al.Compatible GLRa V-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera.Plant Mol Biol,2011,77:261-274.
[3]Gouveia P,Santos M T,Eiras-Dias J E,et al.Five phylogenetic groups identified in the coat protein gene of grapevine leaf roll-associated virus 3 obtained from Portuguese grapevine varieties.Arch Virol,2011,156:413-420.
[4]沙月霞,王国珍,樊仲庆.贺兰山东麓主栽葡萄品种病毒病鉴定及健康状况评价.西北农业学报,2010,19(12):188-193.
[5]单守明,平吉成,刘晶,等.6-BA对染卷叶病毒葡萄光合作用和果实品质的影响.北方园艺,2013(4):5-8.
[6]Pessarakli M.Handbook of photosynthesis(Second Edition).London:CRC Press,2005:169-451.
[7]江新华,谢江辉,王尉,等.水杨酸诱导香蕉抗枯萎病生理试验.中国果树,2006(6):39-42.
[8]朱红芳,高倩倩,李晓锋,等.外源水杨酸(SA)对不结球白菜根肿病的抗性诱导效应,上海农业学报,2017,33(1):63-68.
[9]汪尚,徐鹭芹,张亚仙,等.水杨酸介导植物抗病的研究进展.植物生理学报,2016,52(5):581-590.
[10]杨恕玲,单守明,巩传银,等.水杨酸对茶树休眠期茶树光合作用和抗冻性的影响.中国农学通报,2009,25(15):121-124.
[11]Lee W S,Fu S F,Li Z,et al.Salicylic acid treatment and expression of an RNA-dependent RNA polymerase1 transgene inhibit lethal symptoms and meristem invasion during tobacco mosaic virus infection in Nicotiana benthamiana.BMC Plant Biology,2016,16:15-29.
[12]Rodriguez M C,Conti G,Zavallo D,et al.TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection.BMC Plant Biology,2014,14:210-226.
[13]李卫芳,王忠,韩鹰,等.小麦Rubisco活化酶的纯化及其活性特性.中国农业科学,2002,35(8):929-933.
[14]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000,134-161.
[15]王永章,王小芳,张大鹏.苹果果实转化酶的种类和特性研究.中国农业大学学报,2001,6(5):9-14.
[16]王华.葡萄与葡萄酒实验技术操作规范.西安:西安地图出版社,1999.
[17]夏国海,张大鹏,贾文锁.IAA、GA和ABA对葡萄果实14C蔗糖输入与代谢的调控.园艺学报,2000,27(1):6-10.
[18]陈俊伟,张上隆,张良诚.果实中糖的运输、代谢与积累及其调控.植物生理与分子生物学学报,2004,30(1):1-10.