一株解磷细菌菌株的筛选、鉴定
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摘要
根据不同地区的生态环境,采用广泛性筛选,用解离目标矿物复筛方法,寻找开发出性能优异的具有良好应用前景的解磷细菌。采用该方法,从贵州铜仁万山钾矿产区分离培育出一株解磷菌株,命名为TK.1。通过细菌生理生化测试,形态学观察,16S r DNA同源性分析,NCBI数据库对比分析研究,该菌属于节杆菌属。TK.1菌株与商业菌巨大芽孢杆菌解离煤矸石的对比研究表明:TK.1菌株解磷指标及效果优于巨大芽孢杆菌,是一株性能优异的解磷细菌。新型解磷细菌的开发,使煤矸石有可能成为生产微生物矿物肥料的原料,解决煤矸石污染问题,对环境保护和可持续性发展具有重要意义。
Based on the ecological environment in different areas and extensive screening method,re-screening method of dissociation of the target mineral was adopted to find a phosphate-dissolving bacteria of excellent performance with potential application. By this method,a strain of phosphatesolubilizing bacterium was isolated from a potassium mineral area in Wanshan District of Tongren city in Guizhou province, named TK. 1. Through the bacterial physiological test, morphological observation,16 S r DNA sequence analysis,searching the NCBI database,it was concluded that the bacterium belonged to the genus Arthrobacter. According to the comparison dissociation of coal gangue of strain TK.1 to that of commercial Bacillus megaterium var. phosphaticum,it was obvious that the property and effect of releasing-phosphorus of strain TK.1 were better than the commercial bacillus. Therefore,strain TK.1 is an excellent phosphate solubilizing bacterium. The development of new type of phosphate-solubilizing bacteria makes it possible for coal gangue to be the raw material for the production of microbial mineral fertilizers,and to solve the problem of coal gangue pollution,which is of great significance to environmental protection and sustainable development.
Based on the ecological environment in different areas and extensive screening method,re-screening method of dissociation of the target mineral was adopted to find a phosphate-dissolving bacteria of excellent performance with potential application. By this method,a strain of phosphatesolubilizing bacterium was isolated from a potassium mineral area in Wanshan District of Tongren city in Guizhou province, named TK. 1. Through the bacterial physiological test, morphological observation,16 S r DNA sequence analysis,searching the NCBI database,it was concluded that the bacterium belonged to the genus Arthrobacter. According to the comparison dissociation of coal gangue of strain TK.1 to that of commercial Bacillus megaterium var. phosphaticum,it was obvious that the property and effect of releasing-phosphorus of strain TK.1 were better than the commercial bacillus. Therefore,strain TK.1 is an excellent phosphate solubilizing bacterium. The development of new type of phosphate-solubilizing bacteria makes it possible for coal gangue to be the raw material for the production of microbial mineral fertilizers,and to solve the problem of coal gangue pollution,which is of great significance to environmental protection and sustainable development.
引文
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[12]余贤美,沈奇宾,李炳龙,等.土壤解磷细菌分离和筛选方法的建立[J].热带作物学报,2008,29(3):321-325.
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[15]刘世昌,李彦芹,李凤超,等.一株海水氨氮降解菌的分离、筛选与鉴定[J].河北渔业,2017(4):11-13.
[2]钟传青,黄为一.提高磷矿粉肥效的生物学途径[J].化肥工业,2002,29(2):15-17.
[3]KHAN M S,ZAIDI A,WANIP A.Role of phosphate solubilizing microganisms in sustainable agriculture:a review[J].Agronomy for Sustainable Development,2007,27(1):29-43.
[4]梁绍芬,姜瑞波.解磷微生物肥料的作用和应用[J].土壤肥料,1994(2):46-48.
[5]曹建军,刘永娟,郭广礼.煤矸石的综合利用现状[J].环境污染治理技术与设备,2004,5(1):19-22.
[6]周翠红,常欣.煤矸石综合利用技术综述[J].选煤技术,2007(2):61-64.
[7]钟艳,杨艳梅,谢承卫.利用巨大芽孢杆菌处理高硫和低硫煤矸石制备肥料的研究[J].贵州师范学院学报,2015,31(12):18-25.
[8]袁向芬,谢承卫.利用巨大芽孢杆菌制备高硫煤矸石肥料[J].环境工程学报,2015,9(2):946-950.
[9]袁向芬,程帆,杨艳梅,谢承卫.两种细菌处理高硫煤矸石制备肥料的研究[J].硅酸盐通报,2014,33(2):302-307.
[10]中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985.
[11]LEE M J,JANG S J,LI X M,et al.Comparison of rpo B gene sequencing,16S rRNA gene sequencing,gyr B multiplex PCR,and the VITEK2 system for identification of Acinetobacter clinical isolates[J].Diagnostic Microbiology and Infectious Disease,2014,78(1):29-34.
[12]余贤美,沈奇宾,李炳龙,等.土壤解磷细菌分离和筛选方法的建立[J].热带作物学报,2008,29(3):321-325.
[13]SAITOU N,NEI M.The neighbor-joining method:a new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution,1987,4(4):406-425.
[14]东秀珠,季妙英.常见细菌鉴定手册[M].北京:科学出版社,2001:267-294.
[15]刘世昌,李彦芹,李凤超,等.一株海水氨氮降解菌的分离、筛选与鉴定[J].河北渔业,2017(4):11-13.