金黄色葡萄球菌表面蛋白Ebh致病性的研究进展
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摘要
细菌感染一直以来都是全球医学专家共同面临的一个难题,特别是当越来越多种类的耐药致病菌的出现。金黄色葡萄球菌既是共生菌又是人类病原体,是引起人类感染性疾病的重要病原菌之一,能够引起局部化脓感染,包括肺炎、伪膜性肠炎、心包炎,甚至败血症、脓毒症等全身感染。有研究发现,在金黄色葡萄球菌表面存在着一种巨蛋白,称为Ebh(ECMbinding protein homologue)。Ebh作用于生物膜的黏附、聚集,与其致病性密切相关。该文将对其进行综述。
Bacterial infections have long been a problem faced by medical experts around the world,especially as more and more types of resistant pathogens emerge.Staphylococcus aureus is both a commensal and a human pathogen,and is one of the important pathogens causing human infectious diseases.It can cause localized purulent infections,including pneumonia,pseudomembranous colitis,pericarditis,and even systemic infections such as sepsis and sepsis.Studies have found that there is a giant protein on the surface of Staphylococcus aureus,called Ebh(ECMbinding protein homologue).Ebh acts on the adhesion and aggregation of biofilms and is closely related to its pathogenicity.This article will review the following sections.
Bacterial infections have long been a problem faced by medical experts around the world,especially as more and more types of resistant pathogens emerge.Staphylococcus aureus is both a commensal and a human pathogen,and is one of the important pathogens causing human infectious diseases.It can cause localized purulent infections,including pneumonia,pseudomembranous colitis,pericarditis,and even systemic infections such as sepsis and sepsis.Studies have found that there is a giant protein on the surface of Staphylococcus aureus,called Ebh(ECMbinding protein homologue).Ebh acts on the adhesion and aggregation of biofilms and is closely related to its pathogenicity.This article will review the following sections.
引文
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[5] CROSBY H A,SCHLIEVERT P M,MERRIMAN J A,et al.The Staphylococcus aureus global regulator mgrA modulates clumping and virulence by controlling surface protein expression[J].PLoS Pathogens,2016,12(5):e1005604.
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[12] 李可可,魏莲花,陈晓青,等.金黄色葡萄球菌细胞外基质结合蛋白表达基因ebh检测及分子流行特征[J].中华医院感染学杂志,2018,28(16):2459-2462,2469.LI Keke,WEI Lianhua,CHEN Xiaoqing,et al.Distribution of the extracellular matrix-binding protein encoding gene EBh and its molecular epidemic characteristics in Staphylococcus aureus strains[J].Journal of Nosocomial Infections,2018,28(16):2459-2462,2469.
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[18] MANNA A C,INGAVALE S S,MALONEY M B,et al.Identification of sarV(SA2062),a new transcriptional regulator,is repressed by sarA and mgrA(SA0641) and involved in the regulation of autolysis in Staphylococcus aureus[J].Journal of Bacteriology,2004,186(16):5267-5280.
[19] CHEN P R,BAE T,WILLIAMS W A,et al.An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus[J].Nature Chemical Biology,2006,2(11):591-595.
[20] 何春燕,刘庆中.金黄色葡萄球菌小菌落突变株研究进展及其临床意义[J].中国感染与化疗杂志,2017,17(4):468-473.HE Chunyan,LIU Qingzhong.Research updates and the clinical implications of Staphylococcus aureus small colony variants[J].Chinese Journal of Infection and Chemoterapy,2017,17(4):468-473.
[21] KIM H K,FALUGI F,MISSIAKAS D M,et al.Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion during Staphylococcus aureus infection[J].Proc Natl Acad Sci USA,2016,113(20):5718-5723.
[22] KAJL B C,BECKER K,LFFLER B.Clinical significance and pathogenesis of Staphylococcal small colony variants in persistent infections[J].Clinical Microbiology Reviews,2016,29(2):401-427.
[23] MELTER O,RADOJEVI B.Small colony variants of Staphylococcus aureusr-eview[J].Folia Microbiologica,2010,55(6):548-558.
[24] GARCIA L G,LEMAIRE S,KAHL B C,et al.Antibiotic activity against small-colony variants of Staphylococcus aureus:review of in vitro,animal and clinical data[J].Journal of Antimicrobial Chemotherapy,2013,68(7):1455-1464.
[25] TAN N C,COOKSLEY C M,ROSCIOLI E,et al.Small-colony variants and phenotype switching of intracellular Staphylococcus aureus in chronic rhinosinusitis[J].Allergy,2014,69(10):1364-1371.
[2] GEOGHEGAN J A,FOSTER T J.Cell wall-anchored surface proteins of staphylococcus aureus:many proteins,multiple functions[J].Current Topics in Microbiology & Immunology,2015,409:95-120.
[3] KAISER M L,THOMPSON D J,MALINOSKI D,et al.Epidemiology and risk factors for hospital-acquired methicillin-resistant Staphylococcus aureus among burn patients[J].Journal of Burn Care & Research,2011,32(3):429-434.
[4] CLARKE S R,HARRIS L G,RICHARDS R G,et al.Analysis of Ebh,a 1.1-megadalton cell wall-associated fibronectin-binding protein of Staphylococcus aureus[J].Infection & Immunity,2002,70(12):6680.
[5] CROSBY H A,SCHLIEVERT P M,MERRIMAN J A,et al.The Staphylococcus aureus global regulator mgrA modulates clumping and virulence by controlling surface protein expression[J].PLoS Pathogens,2016,12(5):e1005604.
[6] ETZ H,MINH D B,HENICS T,et al.Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99(10):6573-6578.
[7] SAKAMOTO S,TANAKA Y I,TANAKA I,et al.Electron microscopy and computational studies of Ebh,a giant cell-wall-associated protein from Staphylococcus aureus[J].Biochemical & Biophysical Research Communications,2008,376(2):261-266.
[8] KAHL B C,HEESEMANN J.Impact of Staphylococcus aureus on the pathogenesis of chronic cystic fibrosis lung disease[J].International Journal of Medical Microbiology,2010,300(8):514-519.
[9] WALKER J N,CROSBY H A,SPAULDING A R,et al.(2013) The Staphylococcus aureus ArlRS two-component system is a novel regulator of agglutination and pathogenesis[J].PLoS Pathog,2013,9(12):e1003819.
[10] PAHARIK A E,HORSWILL A R.The Staphylococcal biofilm:adhesins,regulation,and host response[J].Microbiology Spectrum,2016,4(2):1-48.
[11] CHENG A G,MISSIAKAS D,SCHNEEWIND O.The giant protein Ebh is a determinant of Staphylococcus aureus cell size and complement resistance[J].Journal of Bacteriology,2014,196(5):971-981.
[12] 李可可,魏莲花,陈晓青,等.金黄色葡萄球菌细胞外基质结合蛋白表达基因ebh检测及分子流行特征[J].中华医院感染学杂志,2018,28(16):2459-2462,2469.LI Keke,WEI Lianhua,CHEN Xiaoqing,et al.Distribution of the extracellular matrix-binding protein encoding gene EBh and its molecular epidemic characteristics in Staphylococcus aureus strains[J].Journal of Nosocomial Infections,2018,28(16):2459-2462,2469.
[13] CHRISTNER M,FRANKE G C,SCHOMMER N N,et al.The giant extracellular matrix-binding protein of Staphylococcus epidermidis mediates biofilm accumulation and attachment to fibronectin[J].Molecular Microbiology,2010,75(1):187-207.
[14] TENNIFER N W,HEIDI A C,ADAM R S,et al.The Staphylococcus aureus ArIRS two-component system is a novel regulator of agglutination and pathogenesis[J].PLoS Pathog,2013,9(12):e1003819.
[15] 罗东,万腊根,陈开森.金黄色葡萄球菌促凝因子研究进展[J].实验与检验医学,2017,35(3):293-296.LUO Dong,WAN Lagen,CHEN Kaisen.Research progress of coagulating factors of Staphylococcus aureus[J].Experimental and Laboratory Medicine,2017,35(3):293-296.
[16] TONG S Y,DAVIS J S,EICHENBERGER E,et al.Staphylococcus aureus infections:epidemiology,pathophysiology,clinical manifestations,and management[J].Clinical Microbiology Reviews,2015,28(3):603-661.
[17] JI yindu.Methicillin-resistant Staphylococcus aureus(MRSA) protocols[M].New Jersey:Totowa,Humana Press Inc:2017.
[18] MANNA A C,INGAVALE S S,MALONEY M B,et al.Identification of sarV(SA2062),a new transcriptional regulator,is repressed by sarA and mgrA(SA0641) and involved in the regulation of autolysis in Staphylococcus aureus[J].Journal of Bacteriology,2004,186(16):5267-5280.
[19] CHEN P R,BAE T,WILLIAMS W A,et al.An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus[J].Nature Chemical Biology,2006,2(11):591-595.
[20] 何春燕,刘庆中.金黄色葡萄球菌小菌落突变株研究进展及其临床意义[J].中国感染与化疗杂志,2017,17(4):468-473.HE Chunyan,LIU Qingzhong.Research updates and the clinical implications of Staphylococcus aureus small colony variants[J].Chinese Journal of Infection and Chemoterapy,2017,17(4):468-473.
[21] KIM H K,FALUGI F,MISSIAKAS D M,et al.Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion during Staphylococcus aureus infection[J].Proc Natl Acad Sci USA,2016,113(20):5718-5723.
[22] KAJL B C,BECKER K,LFFLER B.Clinical significance and pathogenesis of Staphylococcal small colony variants in persistent infections[J].Clinical Microbiology Reviews,2016,29(2):401-427.
[23] MELTER O,RADOJEVI B.Small colony variants of Staphylococcus aureusr-eview[J].Folia Microbiologica,2010,55(6):548-558.
[24] GARCIA L G,LEMAIRE S,KAHL B C,et al.Antibiotic activity against small-colony variants of Staphylococcus aureus:review of in vitro,animal and clinical data[J].Journal of Antimicrobial Chemotherapy,2013,68(7):1455-1464.
[25] TAN N C,COOKSLEY C M,ROSCIOLI E,et al.Small-colony variants and phenotype switching of intracellular Staphylococcus aureus in chronic rhinosinusitis[J].Allergy,2014,69(10):1364-1371.