小鼠诺如病毒检测方法团体标准的编制
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摘要
随着生命科学的发展,对实验动物质量的要求也越来越高,实验动物标准的不断完善就变得尤为重要,小鼠诺如病毒在实验小鼠中有很高的感染率,小鼠感染后不仅对动物本身造成危害,对实验结果也会产生较大影响。我国的实验动物国家标准目前还未将小鼠诺如病毒纳入常规检查项目,我们参考国内外小鼠诺如病毒的最新研究成果及国外相关标准,编制了小鼠诺如病毒的团体标准。本文介绍了小鼠诺如病毒检测方法团体标准编制的背景、法律法规依据,对标准内容的设置进行了解释。本标准的编制完善了实验动物相关标准,为推动实验动物质量的提高,适应实验动物国际化发展的需求提供了技术支持。
With the increasing need for high-quality lab animals, the continuous improvement of lab animal standards has become particularly important. Murine norovirus(MNV) has a high infection rate in lab mice. MNV infection not only harms the animals themselves, but also has a great impact on the experimental results. The national standards for laboratory animals in China do not yet include murine norovirus in the list of routine examination items. We have compiled group standards for murine norovirus by referring to the latest research results on murine norovirus at home and abroad, along with relevant foreign standards. This paper introduces the background, and legal and regulatory basis for the preparation of murine norovirus group standards and explains the sets for the standard content. The establishment of such standards should improve the quality of lab animals. They can provide technical support for promoting improved quality of lab animals and adapting to the needs of the international development of lab animals.
With the increasing need for high-quality lab animals, the continuous improvement of lab animal standards has become particularly important. Murine norovirus(MNV) has a high infection rate in lab mice. MNV infection not only harms the animals themselves, but also has a great impact on the experimental results. The national standards for laboratory animals in China do not yet include murine norovirus in the list of routine examination items. We have compiled group standards for murine norovirus by referring to the latest research results on murine norovirus at home and abroad, along with relevant foreign standards. This paper introduces the background, and legal and regulatory basis for the preparation of murine norovirus group standards and explains the sets for the standard content. The establishment of such standards should improve the quality of lab animals. They can provide technical support for promoting improved quality of lab animals and adapting to the needs of the international development of lab animals.
引文
[1] Karst SM, Wobus CE, Lay M, et al.STAT1-dependent innate immunity to a Norwalk-like virus[J]. Science, 2003, 299: 1575-1578.
[2] Hsu CC, Riley LK, Wills HM, et al.Persistent infection with and serologic cross-reactivity of three novel murine novoviruses[J]. Comp Med,2006,56(4):247-251.
[3] Henderson KS. Murine norovirus, a recently discovered and highly prevalent viral agent of mice[J]. Lab Anim, 2008, 37(7): 314-320.
[4] Kitajima M, Oka T, Tohya Y, et al. Development of a broadly reactive nested transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence od murine noroviruses in laboratory mice in Japan[J]. Microbiol Immunol, 2009, 53:531-534.
[5] Kim JR, Seok SH, Kim DJ, et al. Prevalence of murine norovirus infection in Korean laboratory animal facilities[J]. Lab Anim Sci, 2010:1-12.
[6] Ohsugi T, Matsuura K, Kawabe S, et al. Natural infection of murine norovirus in conventional and specific pathogen-free laboratory mice[J]. Front Microbiol, 2013, 4, article 12:1-6.
[7] Mahler M, Kohl W. A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in Western Europe[J]. Lab Anim(NY), 2009, 38(5):161.
[8] 袁文,张钰,刘忠华,等. 广东省实验小鼠自然感染鼠诺如病毒的调查[J].中国比较医学杂志,2010,20(2):78-82.
[9] 高洁,贺争鸣. 小鼠诺如病毒荧光定量PCR检测方法的建立及初步应用[J]. 中国比较医学杂志,2016,26(12):70-76.
[10] Xiang ZG, Tian SN, Tong W, et al. MNV primarily surveillance by a recombin- ation VP1-derived ELISA in Beijing area in China[J]. J Immunol Methods, 2014:1-8.
[11] Randall RE, Goodbourn S. Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures[J]. J Gen Virol, 2008, 89(pt 1):1-47.
[12] Shortland A, Chettle J, Archer J, et al. Pathology caused by persistent murine norovirus infection[J]. J Gen Virol, 2014, 95(pt 2):413-422.
[13] Wobus CE, Karst SM, Thackray LB, et al. Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages[J]. PLoS Biol, 2004, 2(12):2076-2084.
[14] Ward JM, Wobus CE, Thackray LB, et al. Pathology of immunodeficient mice with naturally occurring murine norovirus infection[J]. Toxicol Pathol, 2006, 34(6):708-715.
[15] Hsu CC, Piotrowski SL, Meeker SM,et al. Histologic lesions induced by murine norovirus infection in laboratory mice[J]. Vet Pathol, 2016:1-10.
[16] Doom CM,Turula HM,Hill AB. Investigation of the impact of the common animal facility contaminant murine norovirus on experimental murine cytomegalovirus infection[J]. Virology, 2009, 392(2): 153-161.
[17] Lencioni KC, Seamons A, Treuting PM,et al. Murine norovirus: an intercurrent variable in a mouse model of bacteria-induced inflammatory bowel disease[J]. Comp Med, 2008, 58:522-533.
[18] Lencioni KC, Drivdahl R, Seamons A, et al. Lack of effect of murine norovirus infection on a mouse model of bacteria-induced colon cancer[J]. Comp Med, 61(3):219-226.
[19] Kim YG, Park JH, Reimer T, et al. Viral infection augments Nod1/2 signaling to potentiate lethality associated with secondary bacterial infections[J]. Cell Host Microbe, 2011, 9:496-507.
[20] Thepaut M, Grandjean T, Hober D, et al. Protective role of murine norovirus against Pseudomonas aeruginosa acute pneumonia[J]. Vet Res, 2015, 46:91.
[21] Mahler M, Berard M, Feinstein R, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units[J]. Lab Animals, 2014, 48(3):178-192.
[22] 国家质量监督检验检疫总局,国家标准化管理委员会,民政部. 团体标准管理规定(试行)[OL]. http://m.hexun.com/news/2017-12-26/192093452.html,2017.
[23] 李晓波,付瑞,岳秉飞,等. 鼠诺如病毒的体外分离与鉴定[J].中国病毒病杂志,2011,1(4):293-296.
[2] Hsu CC, Riley LK, Wills HM, et al.Persistent infection with and serologic cross-reactivity of three novel murine novoviruses[J]. Comp Med,2006,56(4):247-251.
[3] Henderson KS. Murine norovirus, a recently discovered and highly prevalent viral agent of mice[J]. Lab Anim, 2008, 37(7): 314-320.
[4] Kitajima M, Oka T, Tohya Y, et al. Development of a broadly reactive nested transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence od murine noroviruses in laboratory mice in Japan[J]. Microbiol Immunol, 2009, 53:531-534.
[5] Kim JR, Seok SH, Kim DJ, et al. Prevalence of murine norovirus infection in Korean laboratory animal facilities[J]. Lab Anim Sci, 2010:1-12.
[6] Ohsugi T, Matsuura K, Kawabe S, et al. Natural infection of murine norovirus in conventional and specific pathogen-free laboratory mice[J]. Front Microbiol, 2013, 4, article 12:1-6.
[7] Mahler M, Kohl W. A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in Western Europe[J]. Lab Anim(NY), 2009, 38(5):161.
[8] 袁文,张钰,刘忠华,等. 广东省实验小鼠自然感染鼠诺如病毒的调查[J].中国比较医学杂志,2010,20(2):78-82.
[9] 高洁,贺争鸣. 小鼠诺如病毒荧光定量PCR检测方法的建立及初步应用[J]. 中国比较医学杂志,2016,26(12):70-76.
[10] Xiang ZG, Tian SN, Tong W, et al. MNV primarily surveillance by a recombin- ation VP1-derived ELISA in Beijing area in China[J]. J Immunol Methods, 2014:1-8.
[11] Randall RE, Goodbourn S. Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures[J]. J Gen Virol, 2008, 89(pt 1):1-47.
[12] Shortland A, Chettle J, Archer J, et al. Pathology caused by persistent murine norovirus infection[J]. J Gen Virol, 2014, 95(pt 2):413-422.
[13] Wobus CE, Karst SM, Thackray LB, et al. Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages[J]. PLoS Biol, 2004, 2(12):2076-2084.
[14] Ward JM, Wobus CE, Thackray LB, et al. Pathology of immunodeficient mice with naturally occurring murine norovirus infection[J]. Toxicol Pathol, 2006, 34(6):708-715.
[15] Hsu CC, Piotrowski SL, Meeker SM,et al. Histologic lesions induced by murine norovirus infection in laboratory mice[J]. Vet Pathol, 2016:1-10.
[16] Doom CM,Turula HM,Hill AB. Investigation of the impact of the common animal facility contaminant murine norovirus on experimental murine cytomegalovirus infection[J]. Virology, 2009, 392(2): 153-161.
[17] Lencioni KC, Seamons A, Treuting PM,et al. Murine norovirus: an intercurrent variable in a mouse model of bacteria-induced inflammatory bowel disease[J]. Comp Med, 2008, 58:522-533.
[18] Lencioni KC, Drivdahl R, Seamons A, et al. Lack of effect of murine norovirus infection on a mouse model of bacteria-induced colon cancer[J]. Comp Med, 61(3):219-226.
[19] Kim YG, Park JH, Reimer T, et al. Viral infection augments Nod1/2 signaling to potentiate lethality associated with secondary bacterial infections[J]. Cell Host Microbe, 2011, 9:496-507.
[20] Thepaut M, Grandjean T, Hober D, et al. Protective role of murine norovirus against Pseudomonas aeruginosa acute pneumonia[J]. Vet Res, 2015, 46:91.
[21] Mahler M, Berard M, Feinstein R, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units[J]. Lab Animals, 2014, 48(3):178-192.
[22] 国家质量监督检验检疫总局,国家标准化管理委员会,民政部. 团体标准管理规定(试行)[OL]. http://m.hexun.com/news/2017-12-26/192093452.html,2017.
[23] 李晓波,付瑞,岳秉飞,等. 鼠诺如病毒的体外分离与鉴定[J].中国病毒病杂志,2011,1(4):293-296.