四磨汤口服液中3种成分人血浆蛋白结合率测定
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摘要
目的研究四磨汤口服液中去甲异波尔定、柚皮苷、新橙皮苷与人血浆蛋白的结合率。方法采用平衡透析法测定四磨汤口服液中去甲异波尔定、柚皮苷、新橙皮苷3种成分与人血浆蛋白的结合率,用高效液相色谱法(HPLC)测定透析内、外液中3种成分的质量浓度,计算血浆蛋白结合率。结果去甲异波尔定、柚皮苷和新橙皮苷的线性关系、精密度、提取回收率和稳定性等均符合方法学要求;在低、中、高3个浓度的四磨汤口服液中,去甲异波尔定与人血浆蛋白结合率分别为(73.09±1.79)%、(70.95±0.67)%、(79.07±3.43)%;柚皮苷与人血浆蛋白结合率分别为(72.44±1.45)%、(74.73±0.16)%、(80.83±2.69)%;新橙皮苷与人血浆蛋白结合率分别为(75.71±1.49)%、(76.03±0.39)%、(79.84±3.17)%。结论四磨汤口服液中去甲异波尔定、柚皮苷、新橙皮苷属于中高度结合型成分。
Objective To study the protein-binding rates of Norisoboldine,Naringin,Neohesperidin in Simotang oral liquid with human plasma proteins. Methods Equilibrium dialysis method was conducted to determine the binding rates of 3 components in Simotang oral liquid in vitro. An HPLC method for the determination of the 3 components both in inner and outer dialysis was established and then the protein-binding rates of the components with human plasma proteins were calculated. Results Linear relationship, precision, extraction recovery and stability of Norisoboldine,Naringin,Neohesperidin all complied with the methodological requirements. At the low,middle and high concentrations of Simotang oral liquid, the human plasma protein-binding rates were(73.09±1.79)%,(70.95±0.67)% and(79.07±3.43)% for Norisoboldine,(72.44±1.45)%,(74.73±0.16)% and(80.83±2.69) % for Naringin,(75.71±1.49)%,(76.03±0.39)% and(79.84±3.17)% for Neohesperidin. Conclusion Norisoboldine,Naringin,Neohesperidin in Simotang oral liquid has middle and high capacities in binding to human plasma proteins in vitro.
Objective To study the protein-binding rates of Norisoboldine,Naringin,Neohesperidin in Simotang oral liquid with human plasma proteins. Methods Equilibrium dialysis method was conducted to determine the binding rates of 3 components in Simotang oral liquid in vitro. An HPLC method for the determination of the 3 components both in inner and outer dialysis was established and then the protein-binding rates of the components with human plasma proteins were calculated. Results Linear relationship, precision, extraction recovery and stability of Norisoboldine,Naringin,Neohesperidin all complied with the methodological requirements. At the low,middle and high concentrations of Simotang oral liquid, the human plasma protein-binding rates were(73.09±1.79)%,(70.95±0.67)% and(79.07±3.43)% for Norisoboldine,(72.44±1.45)%,(74.73±0.16)% and(80.83±2.69) % for Naringin,(75.71±1.49)%,(76.03±0.39)% and(79.84±3.17)% for Neohesperidin. Conclusion Norisoboldine,Naringin,Neohesperidin in Simotang oral liquid has middle and high capacities in binding to human plasma proteins in vitro.
引文
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[10]黄秀旺,杨蕾,邓艳平,等.超滤法结合液质联用法测定姜黄素大鼠血浆蛋白结合率[J].海峡药学,2013,25(11):43-46.
[11]朱立静,何书芬,宗阳,等.交泰丸中4种生物碱与人血浆蛋白结合率的测定[J].中国医院药学杂志,2017,37(22):2222-2225.
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[13]刘睿,谢跃生,潘桂湘,等.药物血浆蛋白结合率测定方法的研究进展[J].天津中医药,2007,24(6):526-528.
[14]张倩,戴国梁,居文政,等.丹红注射液7种药效物质基础的人血浆蛋白结合率[J].中国药理学通报,2017,33(5):712-718.
[15]李季泓.柚皮苷的大鼠血浆蛋白结合率研究[J].实用药物与临床,2015,18(5):578-580.
[16]曾华平,陈红,陈丹,等.玳玳果黄酮降脂提取物效应组分血浆蛋白结合特性研究[J].中国中药杂志,2019,44(4):819-825.
[2]赵先平.四磨汤口服液对便秘小鼠肠道细菌多样性的影响[D].长沙:湖南中医药大学,2016.
[3]邓艳玲,肖新云,李丹丹,等.四磨汤口服液对脾虚便秘小鼠非特异性免疫功能的影响[J].中国微生态学杂志,2015,27(12):1373-1375.
[4]鲍慧敏.四磨汤口服液治疗肝郁气滞便秘型肠易激综合征的疗效分析[J].现代诊断与治疗,2013,24(19):4341.
[5]覃江萍,韦益.儿童营养不良与生存干预措施[J].中国卫生产业,2013,10(17):163-165.
[6]卢乾.去甲异波尔定通过cAMP-PKA-NFκB/Notch1途径抑制VEGF诱导内皮细胞迁移[A]//全国中药药理学会联合会学术交流大会论文摘要汇编[C].中国药理学会中药药理专业委员会,2012.
[7]EL-MARASY S A,ABDALLAH H M,EL-SHENAWY S M,et al.Anti-depressant effect of hesperidin in diabetic rats[J].Can J Physiol Pharmacol,2014,92(11):945-952.
[8]WU M,ZHANG H,ZHOU C,et al.Identification of the chemical constituents in aqueous extract of Zhi-Qiao and evaluation of its antidepressant effect[J].Molecules,2015,20(4):6925-6940.
[9]ZHANG X,HAN L,LIU J,et al.Pharmacokinetic study of 7compounds following oral administration of Fructus aurantii to depressive rats[J].Frontiers in Pharmacology,2018,9:131.
[10]黄秀旺,杨蕾,邓艳平,等.超滤法结合液质联用法测定姜黄素大鼠血浆蛋白结合率[J].海峡药学,2013,25(11):43-46.
[11]朱立静,何书芬,宗阳,等.交泰丸中4种生物碱与人血浆蛋白结合率的测定[J].中国医院药学杂志,2017,37(22):2222-2225.
[12]卢来春,蒋学华,杨俊毅,等.阿莫西林对格列美脲血浆蛋白结合率的影响[J].华西药学杂志,2003,18(4):246-250.
[13]刘睿,谢跃生,潘桂湘,等.药物血浆蛋白结合率测定方法的研究进展[J].天津中医药,2007,24(6):526-528.
[14]张倩,戴国梁,居文政,等.丹红注射液7种药效物质基础的人血浆蛋白结合率[J].中国药理学通报,2017,33(5):712-718.
[15]李季泓.柚皮苷的大鼠血浆蛋白结合率研究[J].实用药物与临床,2015,18(5):578-580.
[16]曾华平,陈红,陈丹,等.玳玳果黄酮降脂提取物效应组分血浆蛋白结合特性研究[J].中国中药杂志,2019,44(4):819-825.