肠通对小鼠便秘的有效性研究
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摘要
目的研究肠通对小鼠便秘的有效性。方法用复方地芬诺酯灌胃制备小鼠便秘模型100只,用0.9%NS灌胃制备空白组模型20只,分为肠通高剂量组(10 mg/只)、肠通中剂量组(5 mg/只)、肠通低剂量组(2.5 mg/只)、阳性对照组、模型组和空白组共6组,每组20只。肠通高剂量组、中剂量组、低剂量组用肠通与墨汁等体积混合进行灌胃,0.1 ml/只;阳性对照组将杜密克与墨汁等体积混合灌胃,67 mg/只,0.1 ml/只;模型组和空白组用墨汁灌胃,0.1 ml/只。灌胃25 min后,每组脱颈处死10只测定墨汁推进率,取小肠标本做病理检查。每组存留的10只小鼠观察灌胃后首粒排黑便时间、6 h内排黑便粒数、粪便含水量、有无死亡。结果 (1)肠通对便秘小鼠墨汁推进率的影响:各组小鼠小肠总长度比较,差异无统计学意义(P>0.05);空白组墨汁推进长度、墨汁推进率均大于其余各组,肠通高、中、低剂量组均大于模型组,差异有统计学意义(P<0.05)。(2)肠通对便秘小鼠排便情况的影响:空白组首粒排便时间短于其余各组,差异有统计学意义(P<0.05);模型组6 h排便粒数、排便含水量均少于其余各组,差异有统计学意义(P<0.05)。(3)各组小鼠均未死亡。(4)病理学:各组小肠绒毛排列整齐,结构完整。结论肠通能有效治疗小鼠便秘。
Objective To study the effectiveness of Changtong on constipation in mice. Methods A total of 100 mice constipation models were prepared by intragastric administration of compound diphenoxylate. 20 mice in the blank group were prepared by intragastric administration of 0.9%NS. They were divided into high-dose group(10 mg/mouse),Intestinal medium dose group(5 mg/mouse), intestinal low dose group(2.5 mg/mouse),positive control group, the model group and the blank group, 20 mice in each group. The high-dose group, the middle-dose group and the low-dose group were intragastrically administered with an equal volume of intestinal and ink, 0.1 ml/mouse; the positive control group was mixed with Dumilk and ink in the same volume, 67 mg/mouse. 0.1 ml/mouse; model group and blank group were intragastrically administered with 0.1 ml/mouse. After 25 minutes of gavage, 10 mice in each group were sacrificed to measure the ink propellation rate, and the small intestine specimens were taken for pathological examination. 10 mice in each group were observed for the first squatting time after gavage, the number of black stools in 6 h, the moisture content of the feces, and the presence or absence of death. Results(1)The effect of Changtong on the ink propelling rate of constipation mice: There was no significant difference in the total length of small intestine between the groups(P>0.05); the ink propulsion length and ink propulsion rate of the blank group were larger than the other groups, The high, medium and low dose groups were larger than the model group,the difference was statistically significant(P<0.05).(2)The effect of Changtong on the defecation of constipation mice: The first defecation time in the blank group was shorter than the other groups, the difference was statistically significant(P<0.05); The number of defecation and defecation water content in the model group were lower than those in the other groups at 6 h,the difference was statistically significant( P<0.05).(3)None of the mice in each group died.(4)Pathology: The villi of the small intestines were arranged neatly and the structure was complete. Conclusion Changtong can effectively treat constipation in mice.
Objective To study the effectiveness of Changtong on constipation in mice. Methods A total of 100 mice constipation models were prepared by intragastric administration of compound diphenoxylate. 20 mice in the blank group were prepared by intragastric administration of 0.9%NS. They were divided into high-dose group(10 mg/mouse),Intestinal medium dose group(5 mg/mouse), intestinal low dose group(2.5 mg/mouse),positive control group, the model group and the blank group, 20 mice in each group. The high-dose group, the middle-dose group and the low-dose group were intragastrically administered with an equal volume of intestinal and ink, 0.1 ml/mouse; the positive control group was mixed with Dumilk and ink in the same volume, 67 mg/mouse. 0.1 ml/mouse; model group and blank group were intragastrically administered with 0.1 ml/mouse. After 25 minutes of gavage, 10 mice in each group were sacrificed to measure the ink propellation rate, and the small intestine specimens were taken for pathological examination. 10 mice in each group were observed for the first squatting time after gavage, the number of black stools in 6 h, the moisture content of the feces, and the presence or absence of death. Results(1)The effect of Changtong on the ink propelling rate of constipation mice: There was no significant difference in the total length of small intestine between the groups(P>0.05); the ink propulsion length and ink propulsion rate of the blank group were larger than the other groups, The high, medium and low dose groups were larger than the model group,the difference was statistically significant(P<0.05).(2)The effect of Changtong on the defecation of constipation mice: The first defecation time in the blank group was shorter than the other groups, the difference was statistically significant(P<0.05); The number of defecation and defecation water content in the model group were lower than those in the other groups at 6 h,the difference was statistically significant( P<0.05).(3)None of the mice in each group died.(4)Pathology: The villi of the small intestines were arranged neatly and the structure was complete. Conclusion Changtong can effectively treat constipation in mice.
引文
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[17]范保瑞,张悦,刘红玉,等.国内药食同源的产生与应用[J].医学研究与教育,2018,35(6):52-64.
[18]韦海潮.杜密克治疗功能性便秘的临床研究[J].临床研究,2013,11(32):66-67.
[19]Khodadad A,Sabbaghian M.Role of synbiotics in the treatment of childhood constipation:a double-blind randomized placebo controlled trial[J].Iran J Pediatr,2010,20(4):387-392.
[20]庄梅兴.中医辨证治疗老年性便秘疗效观察[J].临床医药文献电子杂志,2018,5(14):35-36.
[21]Dziedzic K,Szwengiel A,Górecka D,et al.Effect of Wheat Dietary Fiber Particle Size during Digestion In Vitro on Bile Acid, Faecal Bacteria and Short-Chain Fatty Acid Content[J].Plant Foods for Human Nutrition,2016,71(2):1-7.
[2]刘瑞丽,刘佃温,楚慧.通秘丸治疗小鼠实热证便秘的药效学研究[J].中医学报,2015,30(11):1636-1638.
[3]丁晓洁,董正平,孙喜灵.《中药成方制剂》治疗便秘方剂的组方规律与优势病证分析[J].时珍国医国药,2018,29(2):476-479.
[4]常婧舒,李怡.老年便秘的研究进展简述[A]//中华中医药学会老年病分会.中华中医药学会第十二次中医药防治老年病学术研讨会暨老年病防治科研进展学习班会议论文集[C].2014.
[5]陈庆亮,单成钢,朱京斌,等.药食同源食品起源与行业现状分析[J].黑龙江农业科学,2011(7):114-116.
[6]贾红慧,袁洁,李强华,等.实验条件对复方地芬诺酯小鼠便秘模型的影响[J].中药药理与临床,2008,24(4):70-72.
[7]韩春卉,李燕俊,李业鹏,等.车前谷粉对便秘模型小鼠润肠通便作用的研究[J].中国预防医学杂志,2003,04:31-33.
[8]中华人民共和国卫生部.保健食品检验与评价技术规范[S].2003.
[9]杨圣洁,徐月新,潘佳丽.中医治疗老年性便秘临床研究概况[J].甘肃中医药大学学报,2018,35(2):106-109.
[10]徐国会,萧枫,宋花玲,等.老年慢性功能性便秘康复干预的效果[J].中国老年学杂志,2018,38(15):82-84.
[11]李中国.老年性便秘的中医药治疗[J].中医临床研究,2018,10(16):133-135.
[12]陈万般,王子坪.乳果糖口服液联合美常安治疗老年性便秘的临床研究[J].包头医学院学报,2016,32(5):57-58.
[13]辛兴涛,刘璋,张丙贵,等.中西医结合治疗老年性便秘的效果研究[J].临床医学研究与实践,2017,2(3):120,122.
[14]戴小桃,吴咏莉.中药内服配合敷脐疗法治疗老年性便秘的临床观察[J].云南中医中药杂志,2018,39(11):50-51.
[15]张继良.针灸推拿治疗老年性便秘31例临床观察[J].临床医药文献电子杂志,2018,5(21):84.
[16]束樱子,侯勇,张珺.中医药治疗老年性便秘研究进展[J].中医药临床杂志,2018,30(4):761-764.
[17]范保瑞,张悦,刘红玉,等.国内药食同源的产生与应用[J].医学研究与教育,2018,35(6):52-64.
[18]韦海潮.杜密克治疗功能性便秘的临床研究[J].临床研究,2013,11(32):66-67.
[19]Khodadad A,Sabbaghian M.Role of synbiotics in the treatment of childhood constipation:a double-blind randomized placebo controlled trial[J].Iran J Pediatr,2010,20(4):387-392.
[20]庄梅兴.中医辨证治疗老年性便秘疗效观察[J].临床医药文献电子杂志,2018,5(14):35-36.
[21]Dziedzic K,Szwengiel A,Górecka D,et al.Effect of Wheat Dietary Fiber Particle Size during Digestion In Vitro on Bile Acid, Faecal Bacteria and Short-Chain Fatty Acid Content[J].Plant Foods for Human Nutrition,2016,71(2):1-7.