酸性染料比色法测定功劳木总生物碱的含量
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摘要
目的:采用酸性染料比色法测定功劳木中总生物碱的含量。方法:生物碱与酸性染料结合,生成有色离子对,用有机溶剂萃取,测定有机相中有色离子对特征波长处的吸光度,从而可计算其总生物碱含量。结果:显色后在415 nm处有最大吸收,盐酸小檗碱在20.4～61.2μg/mL范围内成线性关系,平均回收率为97.96%,RSD为4.1%(n=5)。结论:该方法灵敏度高、重复性好。
Subjective: Content quality evaluation of Caulis Mahonia and its total alkaloids. Method: Acid dye colorimetry, In pH=5.2 conditions, the alkaloids combined with acid dyes, which generated the ion absorption Spectra clearly redshift, the ion-pair can be quantified by organic solvent extraction, determination of ferrous ion in organic solvents on the characteristic wavelength of absorbance, which then turn off the determination of content of Total alkaloids from Caulis Mahonia, and recovery experiments. Results: The maximum absorption is 415 nm, Berberine hydrochloride as in 20.4～61.2 μg/mL range linear relationship, the average recovery was 97.96 %, RSD was 4.1 %(n=5). Conclusion: The method uses less high sensitivity and good reproducibility.
Subjective: Content quality evaluation of Caulis Mahonia and its total alkaloids. Method: Acid dye colorimetry, In pH=5.2 conditions, the alkaloids combined with acid dyes, which generated the ion absorption Spectra clearly redshift, the ion-pair can be quantified by organic solvent extraction, determination of ferrous ion in organic solvents on the characteristic wavelength of absorbance, which then turn off the determination of content of Total alkaloids from Caulis Mahonia, and recovery experiments. Results: The maximum absorption is 415 nm, Berberine hydrochloride as in 20.4～61.2 μg/mL range linear relationship, the average recovery was 97.96 %, RSD was 4.1 %(n=5). Conclusion: The method uses less high sensitivity and good reproducibility.
引文
[1]国家药典委员会.中华人民共和国药典2015年版一部[S].2015,86.
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[3]迟森森,韩丹,刘斌.宽筋藤总生物碱含量测定方法研究[J].西北药学杂志,2015,30(4):349-351.
[4]姚再明.鸡蛋参的总生物碱含量测定[J].华西药学杂志,1987(4):204.
[5]李小进.雷公藤总生物碱含量的测定[J].山西中医学院学报.2014,15(3):42-43.
[6]姚德中,闵会,何厚洪,等.不同产地浙贝母总生物碱含量测定与比较[J].中国现代应用药学,2014,31(10):1249-1250.
[2]国家中药管理局委员会.中华本草第三册[M].上海:上海科学技术出版社,1999:317.
[3]迟森森,韩丹,刘斌.宽筋藤总生物碱含量测定方法研究[J].西北药学杂志,2015,30(4):349-351.
[4]姚再明.鸡蛋参的总生物碱含量测定[J].华西药学杂志,1987(4):204.
[5]李小进.雷公藤总生物碱含量的测定[J].山西中医学院学报.2014,15(3):42-43.
[6]姚德中,闵会,何厚洪,等.不同产地浙贝母总生物碱含量测定与比较[J].中国现代应用药学,2014,31(10):1249-1250.