不同亲疏水性质纳米氧化铝存在下铜对斜生栅藻毒性效应的研究
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摘要
纳米氧化铝是应用非常广泛的一类人工纳米材料。当它被释放到水环境中后,会与环境中原有的重金属发生相互作用并对其毒性产生影响。文章研究了不同亲疏水性质的纳米氧化铝存在下铜对斜生栅藻的毒性效应,测定了藻细胞的生长抑制、金属积累及超氧化物歧化酶、谷胱甘肽、丙二醛等生化指标的变化。结果表明,纳米氧化铝显著降低了铜离子对藻细胞的生长抑制,藻细胞内铜的积累量及氧化损伤降低。但不同亲疏水性质的纳米氧化铝之间并不存在显著差别。这可能是由于纳米氧化铝对水中的铜离子发生了吸附,间接降低了藻液中的不稳态铜的浓度,从而减轻了铜对藻细胞的毒害。而纳米氧化铝的不同亲疏水性质对吸附水中铜离子的能力没有显著的影响。
Nano-Al_2O_3 is a widely used artificial nanomaterial. When it is released into the aquatic environment,it would interact with the original water pollutants,especially the heavy metals,and may produce an impact on the toxicity effects of the metals. In current study, the copper toxicity in the presence of nano-Al_2O_3 with different surface properties( hydrophilicity and hydrophobicity) towards Scenedesmus obliquus was investigated. The growth inhibition,accumulated copper in Scenedesmus obliquus,as well as superoxide dismutase activity and concentration of glutathione and malondialdehyde in cells were determined. Results showed that the presence of nano-Al_2O_3 reduced the growth inhibition of Scenedesmus obliquus. The accumulation of copper and the level of oxidative stress in algae were also reduced in the presence of nano-Al_2O_3. Different surface properties of nano-Al_2O_3 showed no significant different effect on the toxicity of copper to Scenedesmus obliquus. The decreased toxicity could be attributed to the adsorption of copper onto Al_2O_3 nanoparticles and the subsequent decrease of labile copper concentration in water.
Nano-Al_2O_3 is a widely used artificial nanomaterial. When it is released into the aquatic environment,it would interact with the original water pollutants,especially the heavy metals,and may produce an impact on the toxicity effects of the metals. In current study, the copper toxicity in the presence of nano-Al_2O_3 with different surface properties( hydrophilicity and hydrophobicity) towards Scenedesmus obliquus was investigated. The growth inhibition,accumulated copper in Scenedesmus obliquus,as well as superoxide dismutase activity and concentration of glutathione and malondialdehyde in cells were determined. Results showed that the presence of nano-Al_2O_3 reduced the growth inhibition of Scenedesmus obliquus. The accumulation of copper and the level of oxidative stress in algae were also reduced in the presence of nano-Al_2O_3. Different surface properties of nano-Al_2O_3 showed no significant different effect on the toxicity of copper to Scenedesmus obliquus. The decreased toxicity could be attributed to the adsorption of copper onto Al_2O_3 nanoparticles and the subsequent decrease of labile copper concentration in water.
引文
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[32]KIM J M,BARRS O,MOREL F M M.Bioavailability and Electroreactivity of Zinc Complexed to Strong and Weak Organic Ligands[J].Environmental Science and Technology,2015,49(18):10 894-10 902.
[33]DEVEZ A,GOMEZ E,GILBIN R,et al.Assessment of Copper Bioavailability and Toxicity in Vineyard Runoff Waters by DPASV and Algal Bioassay[J].Science of the Total Environment,2005,348(1):82-92.
[34]HUANG S B,WANG Z J.Application of Anodic Stripping Voltammetry to Predict the Bioavailable/Toxic Concentration of Cu in Natural Water[J].Applied Geochemistry,2003,18(8):1 215-1 223.
[35]STAUBER J L,ANDRADE S,RAMIREZ M,et al.Copper Bioavailability in a Coastal Environment of Northern Chile:Comparison of Bioassay and Analytical Speciation Approaches[J].Marine Pollution Bulletin,2005,50(11):1 363-1 372.
[2]WANG H H,WICK R L,XING B S.Toxicity of Nanoparticulate and Bulk Zn O,Al2O3and Ti O2to the Nematode Caenorhabditis Elegans.[J].Environmental Pollution,2009,157(4):1 171-1 177.
[3]MESARIA T,SEPAI K,PIAZZA V,et al.Effects of Nano Carbon Black and Single-Layer Graphene Oxide on Settlement,Survival and Swimming Behaviour of Amphibalanus Amphitrite Larvae[J].Chemistry and Ecology,2013,29(7):643-652.
[4]SCHWARZENBACH R P,EGLI T,HOFSTETTER TB,et al.Global Water Pollution and Human Health[J].Annual Review of Environment and Resources,2010,35(1):109-136.
[5]LIU L L,FAN W H,LU H T,et al.Effects of the Interaction Between Ti O2with Different Percentages of Exposed{001}Facets and Cu2+on Biotoxicity in Daphnia Magna[J].Scientific Reports,2015,5:111-121.
[6]YAN C Z,YANG F,WANG Z S,et al.Changes in Arsenate Bioaccumulation,Subcellular Distribution,Depuration,and Toxicity in Artemia Salina Nauplii in the Presence of Titanium Dioxide Nanoparticles[J].Environmental Science:Nano,2017,4(6):1 365-1 376.
[7]TAN L Y,HUANG B,XU S,et al.Aggregation Reverses the Carrier Effects of Ti O2Nanoparticles on Cadmium Accumulation in the Waterflea Daphnia Magna[J].Environmental Science and Technology,2017,51(2):932-939.
[8]KREUTZER S,MARTIN L,TRIBOLO C,et al.Environmental Dose Rate Determination Using a Passive Dosimeter:Techniques and Workflow forα-Al2O3:C Chips[J].Geochronometria,2018,45(1):56-67.
[9]HUSSAIN M Z,KHAN U,JANGID R,et al.Hardness and Wear Analysis of Cu/Al2O3Composite for Application in EDM Electrode[J]IOP Conference Series:Materials Science and Engineering,2018,310(1):12-44.
[10]SARKAR D,REDDY B S,BASU B.Implementing Statistical Modeling Approach Towards Development of Ultrafine Grained Bioceramics:Case of Zr O2-Toughened Al2O3[J].Journal of the American Ceramic Society,2018,101(3):1333-1343.
[11]RADWAN A B,MOHAMED A M A.New Electrospun Polystyrene/Al2O3Nanocomposite Superhydrophobic Coatings,Synthesis,Characterization and Application[J].Coatings,2018,8(2):65-81.
[12]尚兴记,裴广斌,王晓卡.纳米氧化铝的制备方法及市场应用分析[J].中国化工贸易,2017,9(13):194-196.SHANG Xingji,PEI Guangbin,WANG Xiaoka.Production and Market Application of Nano-Al2O3[J].China Chemical Trade,2017,9(13):194-196.
[13]ZA??SKA-RADZIWI??M,DOSKOCZ N.DNAChanges in Pseudomonas Putida Induced by Aluminum Oxide Nanoparticles Using RAPD Analysis[J].Desalination and Water Treatment,2016,57(3):1 573-1 581.
[14]龙奕,刘珊珊,王萌等.纳米Al2O3和Cd联合暴露对铜锈环棱螺体内Cd的生物积累和抗氧化酶活性的影响[J].生态毒理学报,2015,10(2):216-223.LONG Yi,LIU Shanshan.,WANG Meng,et al.Effects of Cd and Al2O3-NPs Co-Exposure on Bioaccumulation of Cd and Antioxidase Enzyme Activities in Bellamya Aeroginosa[J],Asia Journal of Ecotoxicology,2015,10(2):216-223.
[15]LI X M,ZHOU S Y,FAN W H.Effect of NanoAl2O3on the Toxicity and Oxidative Stress of Copper Towards Scenedesmus Obliquus[J].International Journal of Environmental Research&Public Health,2016,13(6):129-137.
[16]SWAYAMPRAVA D,SUNANDAN P,BHUVANESH-WARI M,et al.Toxic Effect of Cr(VI)in Presence of n-Ti O2and n-Al2O3Particles Towards Freshwater Microalgae[J].Aquatic Toxicology,2014,146:28-37.
[17]MCNEIL S E.Nanoparticle Therapeutics:A Personal Perspective[J].Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology,2010,1(3):264-271.
[18]SHEN Y,WANG G X,SHEN N,et al.Advances of Research of Hydrophilic/Hydrophobic Surface Effect on Cell Biologic Behaviors in Vitro[J].Journal of Biomedical Engineering,2011,28(6):1 237-1 241.
[19]FAN W H,LIU L L,PENG R S,et al.High Bioconcentration of Titanium Dioxide Nanoparticles in Daphnia Magna Determined by Kinetic Approach[J].Science of the Total Environment,2016,569-570:1 224-1 231.
[20]LU H T,DONG H F,FAN W H,et al.Aging and Behavior of Functional Ti O2Nanoparticles in Aqueous Environment[J].Journal of Hazardous Materials,2016,32(5):113-119.
[21]HUANG Y T,SU C P.High Lipid Content and Productivity of Microalgae Cultivating Under Elevated Carbon Dioxide[J].International Journal of Environmental Science and Technology,2013,11(3):703-710.
[22]YANG W W,LI Y,MIAO A J,et al.Cd2+Toxicity as Affected by Bare Ti O2Nanoparticles and Their Bulk Counterpart[J].Ecotoxicology and Environmental Safety,2012,85(3):44-51.
[23]WILLIAMS P M.Zeta Potential[M].Berlin,Heidelberg:Springer Berlin Heidelberg,2016.
[24]FLORENCE T M.Electrochemical Approaches to Trace Element Speciation in Waters.A Review[J].Analyst,1986,111(5):489-505.
[25]SADIQ I M,PAKRASHI S,CHANDRASEKARAN N,et al.Studies on Toxicity of Aluminum Oxide(Al2O3)Nanoparticles to Microalgae Species:Scenedesmus sp.and Chlorella sp.[J].Journal of Nanoparticle Research,2011,13(8):3 287-3 299.
[26]HARTMANN N B,KAMMER F V D,HOFMANN T,et al.Algal Testing of Titanium Dioxide NanoparticlesTesting Considerations,Inhibitory Effects and Modification of Cadmium Bioavailability[J].Toxicology,2010,269(2):190-197.
[27]QIN H W.,CHEN L F,LU N,et al.Toxic Effects of Enrofloxacin on Scenedesmus Obliquus[J].Frontiers of Environtmental Science and Engineering,2012,6(1):107-116.
[28]NEL A.,XIA T.,MADLER L,et al.Toxic Potential of Materials at the Nanolevel[J].Science,2006,311(5761):622-627.
[29]ALLEN H E,HANSEN D J.The Importance of Trace Metal Speciation to Water Quality Criteria[J].Water Environment Research,1996,68(1):42-54.
[30]ALLEN H E.The Significance of Trace Metal Speciation for Water,Sediment and Soil Quality Criteria and Standards[J].Science of the Total Environment,1993,134(5):23-45.
[31]BATLEY G E,APTE S C,STAUBER J L.Speciation and Bioavailability of Trace Metals in Water:Progress Since 1982[J].Australian Journal of Chemistry,2004,57(10):903-919.
[32]KIM J M,BARRS O,MOREL F M M.Bioavailability and Electroreactivity of Zinc Complexed to Strong and Weak Organic Ligands[J].Environmental Science and Technology,2015,49(18):10 894-10 902.
[33]DEVEZ A,GOMEZ E,GILBIN R,et al.Assessment of Copper Bioavailability and Toxicity in Vineyard Runoff Waters by DPASV and Algal Bioassay[J].Science of the Total Environment,2005,348(1):82-92.
[34]HUANG S B,WANG Z J.Application of Anodic Stripping Voltammetry to Predict the Bioavailable/Toxic Concentration of Cu in Natural Water[J].Applied Geochemistry,2003,18(8):1 215-1 223.
[35]STAUBER J L,ANDRADE S,RAMIREZ M,et al.Copper Bioavailability in a Coastal Environment of Northern Chile:Comparison of Bioassay and Analytical Speciation Approaches[J].Marine Pollution Bulletin,2005,50(11):1 363-1 372.