北京昌平区46株副溶血性弧菌分子流行病学特征分析
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摘要
目的了解北京昌平区副溶血性弧菌临床菌株血清型、耐药性、毒力基因携带情况及分子特征。方法对2017年北京市昌平区分离到的46株副溶血性弧菌进行血清分型、药敏实验、毒力基因检测及脉冲场凝胶电泳分型(PFGE)分析。结果 46株副溶血性弧菌的主要血清型为O3:K6,PFGE分析得到37种PFGE带型,菌株之间的相似系数为59. 9%～100. 0%。本实验中的副溶血性弧菌除头孢唑啉外,对其他16种抗菌素敏感或完全敏感,临床菌株均携带tlh和tdh基因,不携带trh基因。结论昌平区引起腹泻的副溶血性弧菌主要为O3:K6血清型、毒力基因均为tdh(+)/tlh(+)/trh(-)、对多数抗生素敏感,PFGE带型呈高度多态性。
Objective To understand the serotypes,antibiotic resistances,virulence genes and molecular characteristics of Vibrio Parahaemolyticus in Changping district of Beijing. Methods 46 Vibrio Parahaemolyticus strains isolated from Changping district of Beijing in 2017 were analyzed with serotyping,antibiotics susceptibility test,virulence genes detection and pulsed-field gel electrophoresis. Results Among the 46 Vibrio Parahaemolyticus strains,O3 ∶ K6 was the dominant serotype. Thirty-seven PFGE patterns were found with the similarity coefficient from 59. 9% to 100. 0%. Besides cefazolin,Vibrio Parahaemolyticus strains were sensitivity or completely sensitive to the other 16 antibiotics. The gene tlh and tdh were found in all strains isolated from diarrheal cases of human,and the gene trh was not found. Conclusion The dominant serotype of Vibrio Parahaemolyticus strains isolated from diarrheal cases of human in Changping district of Beijing is O3∶ K6,and the strains have the virulence gene tlh and tdh. The strains show sensitivity to almost antibiotics and the PFGE patterns are a high polymorphism.
Objective To understand the serotypes,antibiotic resistances,virulence genes and molecular characteristics of Vibrio Parahaemolyticus in Changping district of Beijing. Methods 46 Vibrio Parahaemolyticus strains isolated from Changping district of Beijing in 2017 were analyzed with serotyping,antibiotics susceptibility test,virulence genes detection and pulsed-field gel electrophoresis. Results Among the 46 Vibrio Parahaemolyticus strains,O3 ∶ K6 was the dominant serotype. Thirty-seven PFGE patterns were found with the similarity coefficient from 59. 9% to 100. 0%. Besides cefazolin,Vibrio Parahaemolyticus strains were sensitivity or completely sensitive to the other 16 antibiotics. The gene tlh and tdh were found in all strains isolated from diarrheal cases of human,and the gene trh was not found. Conclusion The dominant serotype of Vibrio Parahaemolyticus strains isolated from diarrheal cases of human in Changping district of Beijing is O3∶ K6,and the strains have the virulence gene tlh and tdh. The strains show sensitivity to almost antibiotics and the PFGE patterns are a high polymorphism.
引文
[1] Banerjee SK,Kearney AK,Nadon CA,et al. Phenotypic and genotypic characterization of Canadian clinical isolates of vibrio parahaemolyticus collected from 2000 to 2009[J]. Journal of Clinical Microbiology,2014,52(4):1081-1088.
[2]张勇,赖植发,周海涛,等.副溶血性弧菌临床分离株血清型、毒力基因和PFGE分子分型特征分析[J].卫生研究,2013,42(4):619-624.
[3]闫鹏,高红,林文凭,等.副溶血性弧菌多位点序列分型方法的建立与应用[J].中国预防医学杂志,2016,17(2):103-107.
[4]王彦波,李颖,李湛,等. 2016年北京市顺义区食源性疾病主动监测实验室检测结果分析[J].预防医学情报杂志,2018,34(3):344-348.
[5]闻艳红,彭华,徐代庆,等. 2012-2016年北京市昌平区感染性腹泻病原菌检测结果分析[J].现代预防医学,2017,44(13):2444-2447,2452.
[6]舒高林,彭华,闻艳红,等. 2015年北京市昌平区副溶血性弧菌临床菌株分子特征及耐药性研究[J].现代预防医学,2017,44(4):743-745,768.
[7]张兰荣,张扬,周景林,等.通州区水产品污染副溶血性弧菌和其引起腹泻情况的调查研究[J].中国卫生检验杂志,2011,21(3):749-751.
[8]鞠长燕,潘伟光,黄锐敏,等. 2010-2013年广东省深圳市南山区腹泻患者沙门菌血清学和脉冲场凝胶电泳分析[J].疾病监测,2015,30(1):30-34.
[9]陈黎,方晔,陈建才.义乌市33株副溶血性弧菌脉冲场凝胶电泳分子分型结果[J].上海预防医学,2015,27(3):134-136.
[10]效拟,李柏生,刘谢,等.广东省2013年副溶血弧菌暴发与散发菌株的病原学特征[J].中华流行病学杂志,2014,35(12):1379-1383.
[11]李薇薇,梅玲玲,唐震,等. 2007-2009年中国副溶血性弧菌临床分离株分子特征分析[J].中华预防医学杂志,2014,48(1):44-52.
[12]陈海丽,周海健,宰淑蓓,等. 2010-2012年上海市两家哨点医院门诊腹泻病例副溶血性弧菌感染状况及菌株分子特征分析[J].中华预防医学杂志,2015,49(3):233-236.
[13]吴青,韩海红,余东敏,等.北京市水产品污染与感染病例中副溶血性弧菌血清型和毒力基因型的比较研究[J].中国食品卫生杂志,2015,27(4):363-367.
[2]张勇,赖植发,周海涛,等.副溶血性弧菌临床分离株血清型、毒力基因和PFGE分子分型特征分析[J].卫生研究,2013,42(4):619-624.
[3]闫鹏,高红,林文凭,等.副溶血性弧菌多位点序列分型方法的建立与应用[J].中国预防医学杂志,2016,17(2):103-107.
[4]王彦波,李颖,李湛,等. 2016年北京市顺义区食源性疾病主动监测实验室检测结果分析[J].预防医学情报杂志,2018,34(3):344-348.
[5]闻艳红,彭华,徐代庆,等. 2012-2016年北京市昌平区感染性腹泻病原菌检测结果分析[J].现代预防医学,2017,44(13):2444-2447,2452.
[6]舒高林,彭华,闻艳红,等. 2015年北京市昌平区副溶血性弧菌临床菌株分子特征及耐药性研究[J].现代预防医学,2017,44(4):743-745,768.
[7]张兰荣,张扬,周景林,等.通州区水产品污染副溶血性弧菌和其引起腹泻情况的调查研究[J].中国卫生检验杂志,2011,21(3):749-751.
[8]鞠长燕,潘伟光,黄锐敏,等. 2010-2013年广东省深圳市南山区腹泻患者沙门菌血清学和脉冲场凝胶电泳分析[J].疾病监测,2015,30(1):30-34.
[9]陈黎,方晔,陈建才.义乌市33株副溶血性弧菌脉冲场凝胶电泳分子分型结果[J].上海预防医学,2015,27(3):134-136.
[10]效拟,李柏生,刘谢,等.广东省2013年副溶血弧菌暴发与散发菌株的病原学特征[J].中华流行病学杂志,2014,35(12):1379-1383.
[11]李薇薇,梅玲玲,唐震,等. 2007-2009年中国副溶血性弧菌临床分离株分子特征分析[J].中华预防医学杂志,2014,48(1):44-52.
[12]陈海丽,周海健,宰淑蓓,等. 2010-2012年上海市两家哨点医院门诊腹泻病例副溶血性弧菌感染状况及菌株分子特征分析[J].中华预防医学杂志,2015,49(3):233-236.
[13]吴青,韩海红,余东敏,等.北京市水产品污染与感染病例中副溶血性弧菌血清型和毒力基因型的比较研究[J].中国食品卫生杂志,2015,27(4):363-367.