丝状真菌遗传转化系统的最新研究进展
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摘要
随着大量丝状真菌基因组序列的公布,丝状真菌基因功能研究已然成为现今分子生物学的热点领域。真菌的遗传转化是研究基因功能的重要手段,研究者不断优化和开发出新的、高效的遗传转化方法,以满足更多真菌物种研究的需要。本综述总结了丝状真菌的遗传转化体系的最新研究进展,如遗传转化方法、选择标记的类型和转化系统的应用。
With the release of a large number of filamentous fungal genome sequences, the study on gene function of filamentous fungi has gradually become a hotspot field of current molecular biology. The fungi genetic transformation is an essential method for the research of gene functions. Researchers continue to optimize and develop new and efficient genetic transformation methods to meet the needs of more fungal species research. The latest research progress on genetic transformation system of filamentous fungi was summarized in this paper, such as genetic transformation methods, the type of selective markers and the application of the transformation system.
With the release of a large number of filamentous fungal genome sequences, the study on gene function of filamentous fungi has gradually become a hotspot field of current molecular biology. The fungi genetic transformation is an essential method for the research of gene functions. Researchers continue to optimize and develop new and efficient genetic transformation methods to meet the needs of more fungal species research. The latest research progress on genetic transformation system of filamentous fungi was summarized in this paper, such as genetic transformation methods, the type of selective markers and the application of the transformation system.
引文
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Han X.L.,Bai J.K.,Zhang W.,Zhang R.,and Sun G.Y.,2016,PEG-mediated transformation Colletotrichum fructicola,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),25(3):442-449(韩小路,白静科,张玮,张荣,孙广宇,2016,PEG介导的苹果果生刺盘孢Colletotrichum fructicola原生质体转化,西北农业学报,25(3):442-449)
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Huan Y.L.,Ye J.,Jiang X.L.,and Zhu C.X.,2007,Advance in genetic transformation system of fungi,Weishengwuxue Tongbao(Journal of Microbiology),34(6):1213-1217(黄亚丽,叶婧,蒋细良,朱昌雄,2007,真菌遗传转化系统的研究进展,微生物学通报,34(6):1213-1217)
Irie T.,Honda Y.,Watanabe T.,and Kuwahara M.,2001,Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA,Appl.Microbiol.Biot.,55(5):563-565
Katz M.E.,and Hynes M.J.,1989,Characterization of the amd R-controlled lamA and lamB genes of Aspergillus nidulans,Genetics,122(2):331-339
Kong D.Z.,Hua C.L.,Wang X.Z.,Liu B.C.,Zhu J.B.,and Wang A.Y.,2016,dGFP-GUS genes tagged of Verticillium dahliae of cotton in Xinjiang marking,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),53(1):1-8(孔德真,华东来,王秀珍,刘步仓,祝建波,王爱英,2016,dGFP-GUS基因标记新疆棉花黄萎病原菌的研究,新疆农业科学,53(1):1-8)
Kong H.M.,2008,Agrobacterium tumefaciens-mediated transfor mation of Magnaporthe grisea,Thesis for M.S.,Zhejiang University,Supervisor:WANG Y.Z,pp.5-6(孔慧敏,2008,农杆菌介导的稻瘟病菌转化及致病突变体的筛选,硕士学位论文,浙江大学,导师:王逸政,pp.5-6)
Li H.Y.,Pan C.Y.,Chen H.,Zhao C.J.,Lu G.D.,and Wang Z.H.,2003,Optimization of T-DNA insertional mutagenesis and analysis of mutants of Magnaporthe grisea,Shengwu Gongcheng Xuebao(Chinese Journal of Biotechnology),19(4):419-423(李宏宇,潘初沂,陈涵,赵长江,鲁国东,王宗华,2003,稻瘟病菌T-DNA插入方法优化及其突变体分析,生物工程学报,19(4):419-423)
Li J.,Yang J.K.,Liang L.M.,and Zhang K.Q.,2006,Advance in the research on genetic transformation system of filamentous fungi,Jiangxi Nongyedaxue Xuebao(Acta Agriculturae U-niversitatis Jiangxiensis),28(4):516-520(李娟,杨金奎,梁连铭,张克勤,2006,丝状真菌遗传转化系统研究进展,江西农业大学学报,28(4):516-520)
Li L.,Xue C.,Bruno K.,Nishimura M.,and Xu J.R.,2004,Two PAK kinase genes,CHM1 and MST20,have distinct functions in Magnaporthe grisea,Mol.Plant Microbe In.17(5):547-556
Li S.Q.,Yi X.N.,Jia Y.J.,and Zhang C.X.,2014,A simple and high-efficient electroporation method for Penicillium chrysogenum,Zhongguo Kangshengsu Zazhi(Chinese Journal of Antibiotics),39(9):651-653(李树强,仪修南,贾亚娟,张春晓,2014,产黄青霉简单高效遗传转化法---电激转化法,中国抗生素杂志,39(9):651-653)
Li W.,and Zhang Y.Z.,2005,Agrobacterium tumefaciens-mediated transformation of the white-rot basidiomycete,Phanerochaete chrysosporium,Weishengwu Xuebao(Acta Microbiologica Sinica),45(5):784-787(李维,张义正,2005,根癌农杆菌介导的白腐丝状真菌-黄孢原毛平革菌的转化,微生物学报,45(5):784-787)
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