重金属累积对泽兰实蝇幼虫生长发育和保护酶的影响
|
摘要
为了弄清重金属通过寄主植物紫茎泽兰累积后对专性寄生天敌泽兰实蝇的生理影响,采用模拟Cd~(2+)、Pb~(2+)或Zn~(2+)污染的土壤培养法胁迫紫茎泽兰,让泽兰实蝇低龄幼虫寄生取食,利用原子吸收分光光度法测定泽兰实蝇老熟幼虫中Cd~(2+)、Pb~(2+)或Zn~(2+)的含量,同时测定分析泽兰实蝇老熟幼虫的体长、鲜重、能量物质和保护酶的变化。结果表明,实验处理的Cd~(2+)、Pb~(2+)或Zn~(2+)均可通过紫茎泽兰传递并累积到泽兰实蝇老熟幼虫体内,引起老熟幼虫一系列生理变化:长度和鲜重降低;总糖、总蛋白和总脂的含量降低,相应的热量值也降低;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等保护酶的活性降低。本研究为进一步阐明重金属污染对食物链各环节的影响提供了重要的理论依据。
In order to understand the physiological effects of heavy metals accumulated by the host plant Eupatorium adenophorum Spreng on Procecidochares utilis Stone,E.adenophorum was exposed to Cd~(2+),Pb~(2+)or Zn~(2+)respectively in pot experiments,and was parasitized by the low instar larvae of P.utilis.The contents of Cd~(2+),Pb~(2+)or Zn~(2+)in the mature larvae of P.utilis were determined by atomic absorption spectrophotometry.Meanwhile,the body length and fresh weight of the mature larvae were measured and analyzed,and the energy substance and protective enzymes in the mature larvae were measured and analyzed.The results showed that Cd~(2+),Pb~(2+)or Zn~(2+)under experimental concentrations were transferred from E.adenophorum to P.utilis,which brought to physiological changes of mature larvae.The body length and fresh weight of the mature larvae decreased.Meanwhile,the contents of energy substances(sugar,protein and lipid) decreased,and the corresponding heat value also decreases.The activities of protective enzymes(SOD,POD and CAT) decreased in the mature larvae.The findings will provide an important theoretical basis for further clarifying the effects of heavy metal pollution on all aspects of the food chain.
In order to understand the physiological effects of heavy metals accumulated by the host plant Eupatorium adenophorum Spreng on Procecidochares utilis Stone,E.adenophorum was exposed to Cd~(2+),Pb~(2+)or Zn~(2+)respectively in pot experiments,and was parasitized by the low instar larvae of P.utilis.The contents of Cd~(2+),Pb~(2+)or Zn~(2+)in the mature larvae of P.utilis were determined by atomic absorption spectrophotometry.Meanwhile,the body length and fresh weight of the mature larvae were measured and analyzed,and the energy substance and protective enzymes in the mature larvae were measured and analyzed.The results showed that Cd~(2+),Pb~(2+)or Zn~(2+)under experimental concentrations were transferred from E.adenophorum to P.utilis,which brought to physiological changes of mature larvae.The body length and fresh weight of the mature larvae decreased.Meanwhile,the contents of energy substances(sugar,protein and lipid) decreased,and the corresponding heat value also decreases.The activities of protective enzymes(SOD,POD and CAT) decreased in the mature larvae.The findings will provide an important theoretical basis for further clarifying the effects of heavy metal pollution on all aspects of the food chain.
引文
Bai XH,Gao HH,Meng QX,et al.Effects of long-term exposure to heavy metal Zn2+on development and reproduction of aphid,Sitobion avenae(Fabricius)[J].Journal of Northweast A&FUniversity(Nat.Sci.Ed.),2014,42(4):132-136.[白小惠,高欢欢,孟庆祥,等.重金属锌长期胁迫对麦长管蚜生长发育和繁殖的影响[J].西北农林科技大学学报(自然科学版),2014,42(4):132-136]
Barbehenn RV.Gut-based antioxidant enzymes in a polyphagous and a graminivorous grasshopper[J].Journal of Chemical Ecology,2002,28(7):1329-1347.
Bradford MM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J].Analytical Biochemistry,1976,72(12):248-254.
Deng BY,Yuan QS.The method of the improved even three benzene phenol determination of autoxidation superoxide dismutase activity[J].Progress in Biochemistry and Biophysics,1991,18(2):163.[邓碧玉,袁勤生.改良的连苯三酚自氧化测定超氧化物歧化酶活性的方法[J].生物化学和生物物理进展,1991,18(2):163]
Cao HM,Zheng LX,Wei HY.Effects of heavy metal Ni2+on the development and reproductive behavior of Ostrinia furnacalis(Lepidoptera:Pyralidae)[J].Acta Entomologica Sinica,2015,58(6):650-657.[曹红妹,郑丽霞,魏洪义.重金属Ni2+对亚洲玉米螟生长发育和生殖行为的影响[J].昆虫学报,2015,58(6):650-657]
Emre I,Kayis T,Coskun M,et al.Changes inantioxidative enzyme activity,glycogen,lipid,protein,and malondialdehyde content in cadmium-treated Galleria mellonella larvae[J].Annals of the Entomological Society of America,2013,106(3):371-377.
Fang H.Insect Biochemistry Analysis Method[M].Beijing:Agriculture Press,1989.[冯慧.昆虫生物化学分析法[M].北京:农业出版社,1989]
Gao X,Zhu JY,Wu GX,et al.Effects of dietary cadmium in larval stage on fecundity and gonad development of Boettcherisca peregrina(Diptera:Sarcophagidae)[J].Journal of Environmental Entomology,2011,33(1):24-29.[高熹,朱家颖,吴国星,等.幼虫期染镉对棕尾别麻蝇繁殖力及生殖腺发育的影响[J].环境昆虫学报,2011,33(1):24-29]
Graney RL,Giesy JPJ.Effects of long-term exposure to pentaclorophenol on free amino acid pool and energy reserves of the freshwater amphipod Gammarus pseudolimnaeus[J].Ecotoxicology and Environmental Safety,1986,12(3):233-251.
Li B,Zhang ZH.Accumulation of heavy metals research in Eupatorium adenophorum and analysis of ecological restore potential in Lannigou gold deposit of Guizhou Province[J].Gold,2008,29(8):47-50.[李冰,张朝晖.烂泥沟金矿区紫茎泽兰对重金属的富集特性及生态修复分析[J].黄金,2008,29(8):47-50]
Li LJ,Guo YP,Wu WL,et al.Toxic effects of Cd2+on protective enzyme systems in different developing stage of Oxya chinensis(Orthoptera:Acridoidae)[J].Journal of Agro-Environment Science,2010,29(8):1448-1454.[李丽君,郭亚平,武文丽,等.镉对中华稻蝗保护酶系统的毒性效应[J].农业环境科学学报,2010,29(8):1448-1454]
Liu LH,Xie SC,Zhang JH.Studies on the distribution,harmfulness and control of Eupatorium adenophorum Spreng[J].Acta Ecologica Sinica,1985,5(1):1-6.[刘伦辉,谢寿昌,张建华.紫茎泽兰在我国的分布、危害与防除途径的探讨[J].生态学报,1985,5(1):1-6]
Liu XW,Qi CM,Ou Yang CB,et al.Growth and absorption enrichment responses of Eupatorium adenophorum Spreng to lead,cadmium and their combined stress[J].Ecology and Environmental Sciences,2014,23(5):876-873.[刘小文,齐成媚,欧阳灿斌,等.Pb、Cd及其复合污染对紫茎泽兰生长及吸收富集特征的影响[J].生态环境学报,2014,23(5):876-873]
Ma S,Wu GX,Tan Q,et al.Host selection behavior of female adults of Procecidochares utilis(Diptera:Tephritidae)[J].Journal of Biosafety,2015,24(1):26-31.[马沙,吴国星,谭清,等.泽兰实蝇雌成虫的寄主选择行为[J].生物安全学报,2015,24(1):26-31]
Nakamatsu Y,Tanaka T.Venom of ectoparasitoid,Euplectrus sp.near plathypenae(Hymenoptera:Eulophidae)regulates the physiological state of Pseudaletia separata(Lepidoptera:Noctuidae)host as a food resource[J].Journal of Insect Physiology,2003,49(2):149-159.
Qing S.The history and status of the study oncrofton weed(Eupatorium adenophorum Spreng.)a worst worldwide weed[J].Journal of Wuhan Botanical Research,1998,16(4):366-372.[强胜.世界恶性杂草:紫茎泽兰研究的历史及现状[J].武汉植物学研究,1998,16(4):366-372]
Simon LM,Fatrai Z,Jonas DE,et al.Study of peroxide metabolism enzymes during the development of Phaseolus vulgaris[J].Biochemie und Physiologie der Pflanzen,1974,166(5):387-392.
Sun HX,Liu Y,Zhang GR.Effects of heavy metal pollution on insects[J].Acta Entomologica Sinica,2007,50(2):l78-185.[孙虹霞,刘颖,张古忍.重金属污染对昆虫生长发育的影响[J].昆虫学报,2007,50(2):l78-185]
Sun HX,Xia Q,Tang WC,et al.Regulation of energy reserves in the hemolymph of Spodoptera litura Fabricius larvae under nickel stress[J].Acta Entomologica Sinica,2010a,53(4):361-368.[孙虹霞,夏嫱,唐文成,等.Ni2+胁迫对斜纹夜蛾幼虫血淋巴中能量物质水平的适应性调节[J].昆虫学报,2010a,53(4):361-368]
Sun HX,Xia Q,Tang WC,et al.Effects of dietary nickel on the energy reserves in the hemolymph of different developmental stages of Spodoptera litura Fabricius[J].Acta Ecologica Sinica,2010b,30(12):3239-3246.[孙虹霞,夏嫱,唐文成,等.Ni2+对不同发育阶段斜纹夜蛾虫体血淋巴能量物质的影响[J].生态学报,2010b,30(12):3239-3246]
Tang QY,Feng MG.DPS Data Procession System for Practical Statistics[M].Beijing:Science Press,2002:43-92.[唐启义,冯明光.实用统计分析及其DPS数据统计分析系统[M].北京:科学出版社,2002:43-92]
Wang WY,Zhang CH.Mensuration and analysis of heavy metals in Eupatorium adenophorum from Shui Yindong gold deposit in Guizhou[J].Bulletin of Botanical Research,2008,28(6):760-763.[汪文云,张朝晖.贵州水银洞紫茎泽兰重金属元素测定与分析[J].植物研究,2008,28(6):760-763]
Wang JX,Gao X,Ma S,et al.Bioconcentration effects of Cd,Pb and Zn in soil-Eupatorium adenophorum Spreng-Procecidochares utilis Stone system[J].Chinese Journal of Eco-Agriculture,2013,21(7):877-882.[王吉秀,高熹,马沙,等.Cd、Pb和Zn在土壤-紫茎泽兰-泽兰实蝇系统中的生物富集效应研究[J].中国生态农业学报,2013,21(7):877-882]
Wang Y,Li LJ,Zhang YP,et al.Accumulation of chromium in Oxya chinensis and its effects on the antioxidant system[J].Journal of Agro-Environment Science,2010,29(12):2281-2286.[王跃,李丽君,张育平,等.铬在中华稻蝗体内的累积及对抗氧化系统的影响[J].农业环境科学学报,2010,29(12):2281-2286]
Wu GX,Gao X,Ye GY,et al.Effects of dietary copper on the growth,development and reproduction of Boettcherisca peregrina(Diptera:Sarcophagidae)in the parental generation and first filial generation[J].Acta Entomologica Sinica,2007,50(10):1042-1048.[吴国星,高熹,叶恭银,等.取食重金属铜对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J].昆虫学报,2007,50(10):1042-1048]
Wu GX,Ye GY,Hu C,et al.Cadmium effects on growth and development as well as reproduction of Boettcherisca peregrina in the parental generation and first filial generation[J].Journal of Zhejiang University(Agria&Life Sci.),2006,32(1):71-76.[吴国星,叶恭银,胡萃,等.重金属镉对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J].浙江大学学报(农业与生命科学版),2006,32(1):71-76]
Xia Q,Hu XJ,Shu YH,et al.Survival and development of Microplitis bicoloratus Chen on larvae of Snodontera litura Fabricius stressed by heave metal zinc[J].Acta Entomologica Sinica,2006,49(3):387-392.[夏嫱,胡新军,舒迎花,等.在受重金属Zn2+胁迫的斜纹夜蛾幼虫寄主上双斑侧沟茧蜂的生存和发育[J].昆虫学报,2006,49(3):387-392]
Xia Q,Sun HX,Hu XJ.Apoptosis of Spodoptera litura larval hemocytes induced by heavy metal zinc[J].Chinese Science Bulletin,2005,50(24):2856-2860.
Xie C,Wu JW,Guo G,et al.Effect of cadimum on energy materials in hemolymph of Musca domestica larvae[J].Journal of Environment&Health,2013,30(4):304-307.[谢春,吴建伟,国果,等.镉对家蝇幼虫血淋巴能量物质的影响[J].环境与健康杂志,2013,30(4):304-307]
Yang HM.The Toxicity Effect of Cd2+and Cr6+on Oxya chinensis[D].Taiyuan:Shanxi University Master Dissertation,2012.[杨慧敏.镉和铬对中华稻蝗毒性效应的研究[D].太原:山西大学硕士学位论文,2012]
Yang SY,Huang YJ,Zhang M,et al.Ecophysiological effects of heavy metals on insects[J].Acta Entomologica Sinica,2015,58(4):427-436.[杨世勇,黄永杰,张敏,等.重金属对昆虫的生态生理效应[J].昆虫学报,2015,58(4):427-436]
Yuan HX,Zhu F,Qin FJ,et al.Effects of cadmium exposure on the lipid peroxide content and the antioxidant enzyme activity and mRNA expression in the fat body in the silkworm,Bombyx mori[J].Acta Entomologica Sinica,2014,57(2):168-175.[袁红霞,褚峰,秦粉菊,等.镉胁迫对家蚕脂肪体脂质过氧化物含量及抗氧化酶活性和mRNA表达的影响[J].昆虫学报,2014,57(2):168-175]
Zhang YP.The Detoxification Mechanism of Grasshopper Oxya chinensis to Cadmium[D].Taiyuan:Shanxi University Doctor Dissertation,2012.[张育平.中华稻蝗对重金属镉的解毒机制研究[D].太原:山西大学博士学位论文,2012]
Zu YQ,Li Y,Chen JJ,et al.Hyperaccumulation of Pb,Zn and Cd in herbaceous grown on lead zinc mining area in Yunnan,China[J].Environment International,2005,31(5):755-762.
Barbehenn RV.Gut-based antioxidant enzymes in a polyphagous and a graminivorous grasshopper[J].Journal of Chemical Ecology,2002,28(7):1329-1347.
Bradford MM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J].Analytical Biochemistry,1976,72(12):248-254.
Deng BY,Yuan QS.The method of the improved even three benzene phenol determination of autoxidation superoxide dismutase activity[J].Progress in Biochemistry and Biophysics,1991,18(2):163.[邓碧玉,袁勤生.改良的连苯三酚自氧化测定超氧化物歧化酶活性的方法[J].生物化学和生物物理进展,1991,18(2):163]
Cao HM,Zheng LX,Wei HY.Effects of heavy metal Ni2+on the development and reproductive behavior of Ostrinia furnacalis(Lepidoptera:Pyralidae)[J].Acta Entomologica Sinica,2015,58(6):650-657.[曹红妹,郑丽霞,魏洪义.重金属Ni2+对亚洲玉米螟生长发育和生殖行为的影响[J].昆虫学报,2015,58(6):650-657]
Emre I,Kayis T,Coskun M,et al.Changes inantioxidative enzyme activity,glycogen,lipid,protein,and malondialdehyde content in cadmium-treated Galleria mellonella larvae[J].Annals of the Entomological Society of America,2013,106(3):371-377.
Fang H.Insect Biochemistry Analysis Method[M].Beijing:Agriculture Press,1989.[冯慧.昆虫生物化学分析法[M].北京:农业出版社,1989]
Gao X,Zhu JY,Wu GX,et al.Effects of dietary cadmium in larval stage on fecundity and gonad development of Boettcherisca peregrina(Diptera:Sarcophagidae)[J].Journal of Environmental Entomology,2011,33(1):24-29.[高熹,朱家颖,吴国星,等.幼虫期染镉对棕尾别麻蝇繁殖力及生殖腺发育的影响[J].环境昆虫学报,2011,33(1):24-29]
Graney RL,Giesy JPJ.Effects of long-term exposure to pentaclorophenol on free amino acid pool and energy reserves of the freshwater amphipod Gammarus pseudolimnaeus[J].Ecotoxicology and Environmental Safety,1986,12(3):233-251.
Li B,Zhang ZH.Accumulation of heavy metals research in Eupatorium adenophorum and analysis of ecological restore potential in Lannigou gold deposit of Guizhou Province[J].Gold,2008,29(8):47-50.[李冰,张朝晖.烂泥沟金矿区紫茎泽兰对重金属的富集特性及生态修复分析[J].黄金,2008,29(8):47-50]
Li LJ,Guo YP,Wu WL,et al.Toxic effects of Cd2+on protective enzyme systems in different developing stage of Oxya chinensis(Orthoptera:Acridoidae)[J].Journal of Agro-Environment Science,2010,29(8):1448-1454.[李丽君,郭亚平,武文丽,等.镉对中华稻蝗保护酶系统的毒性效应[J].农业环境科学学报,2010,29(8):1448-1454]
Liu LH,Xie SC,Zhang JH.Studies on the distribution,harmfulness and control of Eupatorium adenophorum Spreng[J].Acta Ecologica Sinica,1985,5(1):1-6.[刘伦辉,谢寿昌,张建华.紫茎泽兰在我国的分布、危害与防除途径的探讨[J].生态学报,1985,5(1):1-6]
Liu XW,Qi CM,Ou Yang CB,et al.Growth and absorption enrichment responses of Eupatorium adenophorum Spreng to lead,cadmium and their combined stress[J].Ecology and Environmental Sciences,2014,23(5):876-873.[刘小文,齐成媚,欧阳灿斌,等.Pb、Cd及其复合污染对紫茎泽兰生长及吸收富集特征的影响[J].生态环境学报,2014,23(5):876-873]
Ma S,Wu GX,Tan Q,et al.Host selection behavior of female adults of Procecidochares utilis(Diptera:Tephritidae)[J].Journal of Biosafety,2015,24(1):26-31.[马沙,吴国星,谭清,等.泽兰实蝇雌成虫的寄主选择行为[J].生物安全学报,2015,24(1):26-31]
Nakamatsu Y,Tanaka T.Venom of ectoparasitoid,Euplectrus sp.near plathypenae(Hymenoptera:Eulophidae)regulates the physiological state of Pseudaletia separata(Lepidoptera:Noctuidae)host as a food resource[J].Journal of Insect Physiology,2003,49(2):149-159.
Qing S.The history and status of the study oncrofton weed(Eupatorium adenophorum Spreng.)a worst worldwide weed[J].Journal of Wuhan Botanical Research,1998,16(4):366-372.[强胜.世界恶性杂草:紫茎泽兰研究的历史及现状[J].武汉植物学研究,1998,16(4):366-372]
Simon LM,Fatrai Z,Jonas DE,et al.Study of peroxide metabolism enzymes during the development of Phaseolus vulgaris[J].Biochemie und Physiologie der Pflanzen,1974,166(5):387-392.
Sun HX,Liu Y,Zhang GR.Effects of heavy metal pollution on insects[J].Acta Entomologica Sinica,2007,50(2):l78-185.[孙虹霞,刘颖,张古忍.重金属污染对昆虫生长发育的影响[J].昆虫学报,2007,50(2):l78-185]
Sun HX,Xia Q,Tang WC,et al.Regulation of energy reserves in the hemolymph of Spodoptera litura Fabricius larvae under nickel stress[J].Acta Entomologica Sinica,2010a,53(4):361-368.[孙虹霞,夏嫱,唐文成,等.Ni2+胁迫对斜纹夜蛾幼虫血淋巴中能量物质水平的适应性调节[J].昆虫学报,2010a,53(4):361-368]
Sun HX,Xia Q,Tang WC,et al.Effects of dietary nickel on the energy reserves in the hemolymph of different developmental stages of Spodoptera litura Fabricius[J].Acta Ecologica Sinica,2010b,30(12):3239-3246.[孙虹霞,夏嫱,唐文成,等.Ni2+对不同发育阶段斜纹夜蛾虫体血淋巴能量物质的影响[J].生态学报,2010b,30(12):3239-3246]
Tang QY,Feng MG.DPS Data Procession System for Practical Statistics[M].Beijing:Science Press,2002:43-92.[唐启义,冯明光.实用统计分析及其DPS数据统计分析系统[M].北京:科学出版社,2002:43-92]
Wang WY,Zhang CH.Mensuration and analysis of heavy metals in Eupatorium adenophorum from Shui Yindong gold deposit in Guizhou[J].Bulletin of Botanical Research,2008,28(6):760-763.[汪文云,张朝晖.贵州水银洞紫茎泽兰重金属元素测定与分析[J].植物研究,2008,28(6):760-763]
Wang JX,Gao X,Ma S,et al.Bioconcentration effects of Cd,Pb and Zn in soil-Eupatorium adenophorum Spreng-Procecidochares utilis Stone system[J].Chinese Journal of Eco-Agriculture,2013,21(7):877-882.[王吉秀,高熹,马沙,等.Cd、Pb和Zn在土壤-紫茎泽兰-泽兰实蝇系统中的生物富集效应研究[J].中国生态农业学报,2013,21(7):877-882]
Wang Y,Li LJ,Zhang YP,et al.Accumulation of chromium in Oxya chinensis and its effects on the antioxidant system[J].Journal of Agro-Environment Science,2010,29(12):2281-2286.[王跃,李丽君,张育平,等.铬在中华稻蝗体内的累积及对抗氧化系统的影响[J].农业环境科学学报,2010,29(12):2281-2286]
Wu GX,Gao X,Ye GY,et al.Effects of dietary copper on the growth,development and reproduction of Boettcherisca peregrina(Diptera:Sarcophagidae)in the parental generation and first filial generation[J].Acta Entomologica Sinica,2007,50(10):1042-1048.[吴国星,高熹,叶恭银,等.取食重金属铜对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J].昆虫学报,2007,50(10):1042-1048]
Wu GX,Ye GY,Hu C,et al.Cadmium effects on growth and development as well as reproduction of Boettcherisca peregrina in the parental generation and first filial generation[J].Journal of Zhejiang University(Agria&Life Sci.),2006,32(1):71-76.[吴国星,叶恭银,胡萃,等.重金属镉对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J].浙江大学学报(农业与生命科学版),2006,32(1):71-76]
Xia Q,Hu XJ,Shu YH,et al.Survival and development of Microplitis bicoloratus Chen on larvae of Snodontera litura Fabricius stressed by heave metal zinc[J].Acta Entomologica Sinica,2006,49(3):387-392.[夏嫱,胡新军,舒迎花,等.在受重金属Zn2+胁迫的斜纹夜蛾幼虫寄主上双斑侧沟茧蜂的生存和发育[J].昆虫学报,2006,49(3):387-392]
Xia Q,Sun HX,Hu XJ.Apoptosis of Spodoptera litura larval hemocytes induced by heavy metal zinc[J].Chinese Science Bulletin,2005,50(24):2856-2860.
Xie C,Wu JW,Guo G,et al.Effect of cadimum on energy materials in hemolymph of Musca domestica larvae[J].Journal of Environment&Health,2013,30(4):304-307.[谢春,吴建伟,国果,等.镉对家蝇幼虫血淋巴能量物质的影响[J].环境与健康杂志,2013,30(4):304-307]
Yang HM.The Toxicity Effect of Cd2+and Cr6+on Oxya chinensis[D].Taiyuan:Shanxi University Master Dissertation,2012.[杨慧敏.镉和铬对中华稻蝗毒性效应的研究[D].太原:山西大学硕士学位论文,2012]
Yang SY,Huang YJ,Zhang M,et al.Ecophysiological effects of heavy metals on insects[J].Acta Entomologica Sinica,2015,58(4):427-436.[杨世勇,黄永杰,张敏,等.重金属对昆虫的生态生理效应[J].昆虫学报,2015,58(4):427-436]
Yuan HX,Zhu F,Qin FJ,et al.Effects of cadmium exposure on the lipid peroxide content and the antioxidant enzyme activity and mRNA expression in the fat body in the silkworm,Bombyx mori[J].Acta Entomologica Sinica,2014,57(2):168-175.[袁红霞,褚峰,秦粉菊,等.镉胁迫对家蚕脂肪体脂质过氧化物含量及抗氧化酶活性和mRNA表达的影响[J].昆虫学报,2014,57(2):168-175]
Zhang YP.The Detoxification Mechanism of Grasshopper Oxya chinensis to Cadmium[D].Taiyuan:Shanxi University Doctor Dissertation,2012.[张育平.中华稻蝗对重金属镉的解毒机制研究[D].太原:山西大学博士学位论文,2012]
Zu YQ,Li Y,Chen JJ,et al.Hyperaccumulation of Pb,Zn and Cd in herbaceous grown on lead zinc mining area in Yunnan,China[J].Environment International,2005,31(5):755-762.