温经汤中6种水溶性活性成分的含量测定
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摘要
目的建立高效液相色谱法同时测定温经汤中绿原酸、隐绿原酸、甘草酸、桂皮醛、丹皮酚和吴茱萸碱的含量。方法采用Waters Xselect HSS T3色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱,设置流速为0.8 mL·min~(-1),柱温为25℃,进行全波长扫描。结果绿原酸、隐绿原酸、甘草酸铵、桂皮醛、丹皮酚、吴茱萸碱分别在30.02～150.08,30.07～150.36,68.82～344.08,10.10～50.50,10.21～51.06,10.18～50.90μg·mL~(-1)范围内浓度与峰面积呈良好线性关系,相关系数分别为0.999 9,0.999 8,0.999 6,0.999 9,0.999 5,0.999 5。结论该方法准确简便,稳定可靠,可用于温经汤名方开发的质量控制。
Objective To establish a method for the content determination of six water-soluble ingredients(chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid, cinnamaldehyde, paeonol and evodiamine) in Wenjing decoction by HPLC. Methods The Waters Xselect HSS T3 column(4.6 mm×250 mm, 5 μm) was used, with acetoniteile-0.1% formic acid solution as the mobile phase for gradient elution. The temperature of column was 25 ℃ at a flow rate of 0.8 mL·min~(-1) with full UV wavelength scan. Results Under the above chromatographic conditions, chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid ammonium salt, cinnamaldehyde, paeonol and evodiamine showed good linear relationship within the ranges of 30.02-150.08,30.07-150.36,68.82-344.08,10.10-50.50,10.21-51.06 and 10.18-50.90 μg·mL~(-1), respectively. The correlation coefficient was 0.999 9, 0.999 8, 0.999 6, 0.999 9, 0.999 5 and 0.999 5, respectively. Conclusion This method is accurate, simple, stable and reliable, and it could be taken as an effective method for controlling the quality of Wenjing decoction development.
Objective To establish a method for the content determination of six water-soluble ingredients(chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid, cinnamaldehyde, paeonol and evodiamine) in Wenjing decoction by HPLC. Methods The Waters Xselect HSS T3 column(4.6 mm×250 mm, 5 μm) was used, with acetoniteile-0.1% formic acid solution as the mobile phase for gradient elution. The temperature of column was 25 ℃ at a flow rate of 0.8 mL·min~(-1) with full UV wavelength scan. Results Under the above chromatographic conditions, chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid ammonium salt, cinnamaldehyde, paeonol and evodiamine showed good linear relationship within the ranges of 30.02-150.08,30.07-150.36,68.82-344.08,10.10-50.50,10.21-51.06 and 10.18-50.90 μg·mL~(-1), respectively. The correlation coefficient was 0.999 9, 0.999 8, 0.999 6, 0.999 9, 0.999 5 and 0.999 5, respectively. Conclusion This method is accurate, simple, stable and reliable, and it could be taken as an effective method for controlling the quality of Wenjing decoction development.
引文
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[13] 裴贵珍,郭鑫,张雪峰,等.HPLC法同时测定六味能消胶囊中木香烃内酯、去氢木香烃内酯大黄素、大黄酚的含量[J].药物分析杂志,2015,35(2):241-245.
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[15] 薛斐然,周贝.日本汉方制剂对我国经典名方注册监管的启示[J].世界科学技术:中医药现代化,2017,19(4):587-589.
[16] 林晓斐.古代经典名方遴选原则公开征求意见[J].中医药管理杂志,2017,25(6):163.
[17] 陈畅,程锦堂,刘安.经典名方研发策略[J].中国中药杂志,2017,42(9):1814-1818.
[2] 徐丁洁,张碧溦,杜晨光,等.金匮温经汤对虚寒证原发性痛经患者微炎症状态的影响[J].云南中医学院学报,2016,39(3):64-67.
[3] 曾革凤,闵国斌,刘晓华.温经汤加减治疗原发性痛经临床观察[J].新中医,2015,47(3):171-172.
[4] 袁鹏飞,刘焕,尚明英,等.桂枝汤中9种主要成分含量的测定和不同煎煮方式的比较研究[J].中国药房,2016,27(6):801-805.
[5] 刘红波,唐志书,张婷,等.牡丹皮中丹皮酚的提取及包合工艺研究[J].中南药学,2016,14(2):166-169.
[6] 陈华,于波涛,金伟华,等.咽炎灵颗粒中药材的薄层色谱鉴别和甘草酸铵的测定[J].华西药学杂志,2016,31(1):79-81.
[7] 刘湘丹,蔡嘉洛,邹君华,等.吴茱萸及其炮制品中吴茱萸碱、吴茱萸次碱和柠檬苦素成分含量对比分析[J].中医药导报,2016,22(3):39-41.
[8] LI Y,SHEN D,TANG X,et al. Chlorogenic acid prevents isoproterenol-induced hypertrophy in neonatal rat myocytes[J]. Toxicol Lett,2014,226(3):257-263.
[9] OBOH G,AGUNLOYE O M,AKINYEMI A J,et al.Comparative study on the inhibitory effect of caffeic and chlorogenic acids on key enzymes linked to Alzheimer's disease and some pro-oxidant induced oxidative stress in rats' brain-in vitro[J]. Neurochem Res,2013,38(2):413-419.
[10] MA Y,GAO M,LIU D.Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice[J]. Pharm Res,2015,32(4):1200-1209.
[11] 孙世发.名方配伍分析及应用[M].北京:人民卫生出版社,2002:483-486.
[12] 昝珂,黄莉莉,过立农,等.基于特征图谱及多指标成分含量的冬虫夏草野生与人工繁育品比较研究[J].中国中药杂志,2017,42(20):3957-3962.
[13] 裴贵珍,郭鑫,张雪峰,等.HPLC法同时测定六味能消胶囊中木香烃内酯、去氢木香烃内酯大黄素、大黄酚的含量[J].药物分析杂志,2015,35(2):241-245.
[14] 刘筱筱,夏忠庭,何毅,等.远志UPLC多指标成分的测定及指纹图谱研究[J].中草药,2016,47(12):2167-2174.
[15] 薛斐然,周贝.日本汉方制剂对我国经典名方注册监管的启示[J].世界科学技术:中医药现代化,2017,19(4):587-589.
[16] 林晓斐.古代经典名方遴选原则公开征求意见[J].中医药管理杂志,2017,25(6):163.
[17] 陈畅,程锦堂,刘安.经典名方研发策略[J].中国中药杂志,2017,42(9):1814-1818.