摘要
本研究为探讨辣椒素(CAP)对大鼠胃缺血再灌注损伤(GI-R)的作用及机制。成年雄性健康SD大鼠40只,随机分为4组,每组10只,A组(空白对照组),B组(手术组),C组(CAP组),D组(CAP+手术组)。给予A组、B组大鼠灌胃生理盐水10 m L/kg/d,C组、D组大鼠灌胃CAP1 mg/kg/d。4周后B组、D组于GI-R模型建立后24小时处死大鼠,收集胃液测定胃酸总酸度,肉眼计数胃黏膜损伤指数,行胃窦组织病理学观察,检测胃黏膜辣椒素受体(TRPV1)、降钙素基因相关肽(CGRP)的表达,检测胃窦组织丙二醛(MDA)、超氧化物歧化酶(SOD)的水平。结果显示A组、C组胃黏膜未见损伤;与B组相比,D组胃黏膜损伤程度显著减轻(P<0.05),胃酸总酸度显著降低(P<0.05),MDA值显著降低(P<0.05),SOD值显著升高(P<0.05),TRPV1、CGRP表达显著增多(P<0.05)。实验结果表明预先给予CAP 1 mg/kg/d连续灌胃4周具有预防缺血再灌注致大鼠胃黏膜损伤的作用,其机制可能与CAP上调TRPV1、CGRP在胃内的表达、减轻氧自由基损伤有关。
This study was to explore the effect and mechanism of capsaicin on gastric ischemia-reperfusion injury in rats.Specific methods were as follows: 40 SD rats were randomly divided into 4 groups with 10 rats in each group,group A( control group),group B( operation group),group C( CAP group) and group D( CAP + operation group). Rats in group A and group B were treated with intragastric administration of normal saline 10 ml/kg/d,group C and group D with intragastric administration of CAP1 mg/kg/d. After 4 weeks,the rats in group B and group D were killed 24 hours later after the establishment of GI-R model. Last,astric juice was collected to determine the total acidity of gastric acid,counted the gastric mucosal injury index,observed the gastric mucosa pathological injury,and detected the expression of TRPV1、CGRP、MDA、SOD.Results show that: the gastric mucosa of group A and C had no damage. Compared with group B,the gastric mucosa injury were significantly reduced( P < 0. 05),total acidity decreased significantly( P < 0. 05),MDA decreased significantly( P < 0. 05),SOD increased significantly( P < 0. 05) and the expression of TRPV1、CGRP increased significantly( P < 0. 05) in group D. Experimental results show that: it could prevent that GI-R induced acute gastric mucosal injury in rats by pretreated with CAP 1 mg/kg/d for 4 weeks. The mechanism may be related to the up regulation of TRPV1 and CGRP expression in the stomach and the reduction of oxygen free radical damage by CAP.
引文
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