胃癌组织中Vav1蛋白表达与胃癌临床生物学行为及预后的关系
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摘要
目的:探讨胃癌组织中Vav1蛋白表达与肿瘤临床生物学行为及预后的关系,并对Vav1蛋白与转移相关蛋白MMP-2、TIMP-1的关系进行分析。方法:采用免疫组织化学方法检测Vav1、MMP-2、TIMP-1蛋白在胃癌及癌旁组织中的表达,收集患者的临床资料并进行随访。结果:Vav1、MMP-2蛋白在胃癌组织中的阳性表达率高于癌旁组织(均P<0.01),TIMP-1蛋白在胃癌组织中表达低于癌旁组织(P<0.01)。Vav1蛋白与肿瘤分化程度、浸润深度、淋巴结转移、TNM分期相关(P<0.05)。Vav1阳性表达患者5年生存率为26.26%,明显低于阴性表达患者(50.82%)(均P<0.05)。Cox比例风险回归显示,Vav1阳性表达是影响患者预后的独立危险因素(P<0.05)。Vav1与MMP-2存在正相关关系(P<0.05)。结论:Vav1与胃癌的侵袭转移有关,Vav1阳性表达是胃癌预后差的标志。
Objective: To explore the relationship between the expression of Vav1 protein and clinical biological behavior,prognosis of gastric cancer,and to analysize of the relationship among Vav1 protein MMP-2 and TIMP-1 of the metastasis associated protein. Methods: Expressions of Vav1,MMP-2,TIMP-1 proteins in gastric cancer and paracancerous tissues were tested by immunohistochemistry. Data of clinicopathological characteristics and follow-up were also collected. Results: The expressions of Vav1,MMP-2 in gastric cancer were higher than in paracancerous tissue( all P < 0. 01),whereas expression of TIMP-1 was lower in gastric cancer than in paracancerous tissues( P <0. 01). The expression of Vav1 in gastric cancer was correlated with the tumor differentiation,tumor infiltration depth,lymph node metastasis and TNM stage( all P < 0. 05). Five years survival rate in patients with Vav1 positive was 26. 26%,and the negative was 50. 82%( P < 0. 05). Cox proportional hazard regression showed that the positive expression of Vav1 was an independent risk factor affecting the prognosis of patients( P < 0. 05). The expression of Vav1 was positive correlated with MMP-2( P < 0. 05). Conclusion: Vav1 is involved in the invasion,metastasis of gastric cancer,and positive expression of Vav1 may be an independent risk factor for the prognosis of patients with gastric cancer.
Objective: To explore the relationship between the expression of Vav1 protein and clinical biological behavior,prognosis of gastric cancer,and to analysize of the relationship among Vav1 protein MMP-2 and TIMP-1 of the metastasis associated protein. Methods: Expressions of Vav1,MMP-2,TIMP-1 proteins in gastric cancer and paracancerous tissues were tested by immunohistochemistry. Data of clinicopathological characteristics and follow-up were also collected. Results: The expressions of Vav1,MMP-2 in gastric cancer were higher than in paracancerous tissue( all P < 0. 01),whereas expression of TIMP-1 was lower in gastric cancer than in paracancerous tissues( P <0. 01). The expression of Vav1 in gastric cancer was correlated with the tumor differentiation,tumor infiltration depth,lymph node metastasis and TNM stage( all P < 0. 05). Five years survival rate in patients with Vav1 positive was 26. 26%,and the negative was 50. 82%( P < 0. 05). Cox proportional hazard regression showed that the positive expression of Vav1 was an independent risk factor affecting the prognosis of patients( P < 0. 05). The expression of Vav1 was positive correlated with MMP-2( P < 0. 05). Conclusion: Vav1 is involved in the invasion,metastasis of gastric cancer,and positive expression of Vav1 may be an independent risk factor for the prognosis of patients with gastric cancer.
引文
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[8]FERNANDEZ-ZAPICO M E,GONZALEZ-PAZ N C,WEISS E,et al.Ectopic expression of Vav1 reveals an unexpected role in pancreatic cancer tumorigenesis[J].Cancer Cell,2005,7(1):39-49.
[9]齐瑶,李润美,于津浦,等.Vav1蛋白在非小细胞肺癌浸润T淋巴细胞中的表达及意义[J].中国免疫学杂志,2013,29(3):279-282.
[2]MANES T D,POBER J S.TCR-driven transendothelial migration of human effector memory CD4 T cells involves Vav,Rac,and myosinⅡA[J].J Immunol,2013,190(7):3079-3088.
[3]LAZER G,KATZAV S.Guanine nucleotide exchange factors for Rho Gtpases:good therapeutic targets for cancer therapy?[J].Cell Signal,2011,23(6):969-979.
[4]聂鲁愚,朱海涛,谢敏.胃癌患者外周血骨髓干细胞水平变化的初步研究[J].东南大学学报:医学版,2016,35(2):226-228.
[5]姚远,李艳,赵鸿梅.MTA1在胃癌细胞系和胃癌组织中的表达定位以及临床意义[J].东南大学学报:医学版,2012,31(2):158-161.
[6]KATZAV S.Flesh and blood:The story of Vav1,a gene that signals in hematopoietic cells but can be transforming in human malignancies[J].Cancer Lett,2007,255(2):241-254.
[7]SEBBAN S,FARAGO M,GASHAI D,et al.Vav1 fine tunes p53control of apoptosis versus proliferation in breast cancer[J].PLos One,2013,8(1):e54321.
[8]FERNANDEZ-ZAPICO M E,GONZALEZ-PAZ N C,WEISS E,et al.Ectopic expression of Vav1 reveals an unexpected role in pancreatic cancer tumorigenesis[J].Cancer Cell,2005,7(1):39-49.
[9]齐瑶,李润美,于津浦,等.Vav1蛋白在非小细胞肺癌浸润T淋巴细胞中的表达及意义[J].中国免疫学杂志,2013,29(3):279-282.