不同干燥工艺对参蒲盆炎颗粒浸膏中芍药苷、虎杖苷和延胡索乙素的影响
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摘要
目的从指标性化学成分量变化的角度,考察不同干燥方式和条件对参蒲盆炎颗粒浸膏质量的影响,为干燥工艺的优选提供方法依据。方法参蒲盆炎颗粒浸膏分别采用带式真空干燥、喷雾干燥、板式减压干燥、常压干燥4种不同方式进行干燥,测定各干燥样品含水量及芍药苷、虎杖苷和延胡索乙素的量,分别采用方差分析、聚类分析与主成分分析方法比较各组样品中3种指标成分量的差异、聚类情况,确定与干燥工艺参数相关性最大的化学成分。结果不同干燥方式样品含水量差异小,芍药苷、虎杖苷、延胡索乙素的量存在较大差异,以带式真空干燥样品中各成分量最高,喷雾干燥次之,100℃常压干燥样品最低。其中,带式真空干燥、喷雾干燥对样品指标性成分破坏较小且聚为一类,而板式减压干燥、常压干燥对样品指标成分破坏较大,并各自聚为一类。芍药苷、虎杖苷和延胡索乙素的量对4种干燥工艺的参数变化均较为敏感,在干燥过程中应重点监测这几类物质的量变化。结论干燥方式和条件的改变对参蒲盆炎颗粒浸膏质量的影响不容忽视,指标性化学成分定量测定可作为评价参蒲盆炎颗粒浸膏干燥工艺优选的一种快速检测方法。
Objective From the viewpoint of content changes of chemical composition, the effects of different drying methods and conditions on the quality of extracts from Shenpu Penyan Granule(SPG) were investigated. Evidence was provided for the optimization of dessication technology. Methods Extracts from SPG were dried by belt vacuum drying, spray drying, plank decompression drying, and drying under normal pressure, respectively. The moisture capacity and contents of peoniflorin, polygonin and tetrahydropulmatine in samples by different drying methods were determined. The content differences of three indexes chemical constituents and the clustering conditions in different groups of samples were compared by analysis of variance and cluster analysis. The maximal correlation between the components and drying technology parameters was determined by principal component analysis. Results The moisture capacity of different dry extracts were similar to each other. The contents of peoniflorin, polygonin, and tetrahydropulmatine in samples by different drying methods were obviously different. Those components in samples prepared by belt vacuum drying were the highest, those by spray drying were the second highest, and those by drying under normal pressure at 100 ℃were the lowest. Cujus, the index chemical constituents in samples were destroyed minorly by belt vacuum drying and spray drying, while those were destroyed majorly by plank decompression drying and drying under normal pressure. The samples prepared by belt vacuum drying and spray drying were classified in the same group, while those prepared by plank decompression drying and drying under normal pressure were classified respectively in different groups. The contents of peoniflorin, polygonin, and tetrahydropulmatine were sensitive to the technology parameters change of four kinds drying methods, it was important to monitor the content change of the materies. Conclusion The effects of different drying methods and conditions on the quality of extracts from SPG should not be ignored. Determination of the index chemical constituents is a rapid detection method to evaluate the optimization of drying technology for extracts from SPG.
Objective From the viewpoint of content changes of chemical composition, the effects of different drying methods and conditions on the quality of extracts from Shenpu Penyan Granule(SPG) were investigated. Evidence was provided for the optimization of dessication technology. Methods Extracts from SPG were dried by belt vacuum drying, spray drying, plank decompression drying, and drying under normal pressure, respectively. The moisture capacity and contents of peoniflorin, polygonin and tetrahydropulmatine in samples by different drying methods were determined. The content differences of three indexes chemical constituents and the clustering conditions in different groups of samples were compared by analysis of variance and cluster analysis. The maximal correlation between the components and drying technology parameters was determined by principal component analysis. Results The moisture capacity of different dry extracts were similar to each other. The contents of peoniflorin, polygonin, and tetrahydropulmatine in samples by different drying methods were obviously different. Those components in samples prepared by belt vacuum drying were the highest, those by spray drying were the second highest, and those by drying under normal pressure at 100 ℃were the lowest. Cujus, the index chemical constituents in samples were destroyed minorly by belt vacuum drying and spray drying, while those were destroyed majorly by plank decompression drying and drying under normal pressure. The samples prepared by belt vacuum drying and spray drying were classified in the same group, while those prepared by plank decompression drying and drying under normal pressure were classified respectively in different groups. The contents of peoniflorin, polygonin, and tetrahydropulmatine were sensitive to the technology parameters change of four kinds drying methods, it was important to monitor the content change of the materies. Conclusion The effects of different drying methods and conditions on the quality of extracts from SPG should not be ignored. Determination of the index chemical constituents is a rapid detection method to evaluate the optimization of drying technology for extracts from SPG.
引文
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[21]金城.基于理化-生物关联检测的中药(板蓝根)生产过程含量控制方法初步研究[D].北京:中国人民解放军军事医学科学院,2009.
[22]王强.中药制剂工艺有无实质性改变的早期快速检(II)-不同干燥工艺大黄水提物理化性质和生物活性考察[D].北京:中国人民解放军军事医学科学院,2008.
[23]刘长龙,李云霄,钱俊,等.响应面法优化十味盆安颗粒真空带式干燥工艺研究[J].中草药,2015,46(13):1914-1919.
[2]丁冬梅,张振海,蒋艳荣,等.丹参酮IIA喷雾干燥粉体药剂学性质的研究[J].中草药,2014,45(10):1398-1401.
[3]周扬,刘力,徐德生,等.纳米喷雾干燥技术用于生地黄低聚糖微粉的制备工艺研究[J].中草药,2016,47(1):65-71.
[4]刘霖,刘汉清,刘嘉,等.黄葵胶囊中间体稠膏不同干燥方法的对比研究[J].中成药,2014,36(1):195-198.
[5]罗云.中药制剂工艺有无实质性改变的早期快速检(I)—板蓝根水提液不同干燥工艺的差异性研究[D].成都:成都中医药大学,2007.
[6]董娟娥,龚明贵,梁宗锁,等.干燥方法和提取温度对板蓝根、大青叶有效成分的影响[J].中草药,2008,39(1):111-114.
[7]胡彦君,王雅琪,李冰涛,等.板蓝根制剂制备过程中成分变化及其药效相关性研究[J].中草药,2016,47(9):1515-1519.
[8]王学成,伍振峰,王雅琪,等.中药丸剂干燥工艺、装备应用现状及问题分析[J].中草药,2016,47(13):2365-2372.
[9]刘长龙,李云霄,钱俊,等.响应面法优化十味盆安颗粒虎杖醋延胡索的提取工艺研究[J].世界科学技术—中医药现代化,2016,18(9):1602-1608.
[10]刘长龙,李云霄,钱俊,等.响应面法优化十味盆安颗粒真空带式干燥工艺研究[J].中草药,2015,46(13):1914-1919.
[11]钱俊,林夏,王星星,等.双波长HPLC法同时测定妇科IV颗粒剂中芍药苷和虎杖苷的含量[J].药学与临床研究,2014,22(3):219-221.
[12]董燕,贾李蓉,张竹绿.中医药数据标准化现状与数据元研究[J].中国数字医学,2012,7(1):44-47.
[13]苏子仁,陈建南.中药制剂工艺过程的物理化学变化研究[J].中国中药杂志,1998,23(11):671-674.
[14]李润美.岗梅总皂昔及其制剂的制备工艺研究[D].广州:广州中医药大学,2010.
[15]韦熹苑,俞洁东,卢小玲.不同干燥工艺虎杖提取物UPLC指纹图谱的研究[J].广东化工,2016,43(6):26-27.
[16]肖小河,金城,赵中振,等.论中药含量控制与评价模式的创新与发展[J].中国中药杂志,2007,32(14):1377-1381.
[17]徐冰.中药制剂生产过程全程优化方法学研究[D].北京:北京中医药大学,2013.
[18]贺帅,姚育法,周本杰,等.不同干燥工艺对热毒清颗粒中指标成分保留率的影响[J].中国药房,2013,24(31):2916-2919.
[19]洪伟.玄参干燥过程主要活性成分的变化[D].武汉:湖北中医药大学,2012.
[20]叶殷殷.大黄配方颗粒制备工艺及含量标准的研究[D].广州:广州中医药大学,2010.
[21]金城.基于理化-生物关联检测的中药(板蓝根)生产过程含量控制方法初步研究[D].北京:中国人民解放军军事医学科学院,2009.
[22]王强.中药制剂工艺有无实质性改变的早期快速检(II)-不同干燥工艺大黄水提物理化性质和生物活性考察[D].北京:中国人民解放军军事医学科学院,2008.
[23]刘长龙,李云霄,钱俊,等.响应面法优化十味盆安颗粒真空带式干燥工艺研究[J].中草药,2015,46(13):1914-1919.