摘要
针对西非安哥拉回国的不明原因发热病人,筛查其可能携带的传染病病原体。采用荧光PCR的方法对口岸重点关注的黄热病毒、寨卡病毒、登革病毒等病原体进行筛查;采用深度测序的方法,搜索病毒库、细菌库、寄生虫库,进行序列比对筛查各类病原体,采用全基因组测序的方法获得病原体全基因组序列。2017年1月2日凌晨1点,上海出入境检验检疫局国家国境口岸卫生监督检测重点实验室接到一份紧急样本,样本来自1名安哥拉回国的28岁男子。该男子在安哥拉当地医院初筛黄热病阳性,该男子要求自行回国治疗。针对病人的血液样本和尿液样本,开展黄热病毒、登革病毒、寨卡病毒、裂谷热病毒、基孔肯雅病毒、西尼罗病毒、流行性乙型脑炎病毒、疟原虫等多种虫媒病原体的荧光PCR检测,结果显示阴性。通过对血液样本的深度测序发现,该病人感染了一种新型的弹状病毒,中文命名为库波热病毒,并获得了库波热病毒全基因组序列,与尼日利亚发现的库波热病毒比较,同源性为96%。
We screened pathogens causing unexplained acute febrile illness in an individual who returned to China from Angola.Quantitative real-time polymerase chain reaction was performed to detect 14 pathogens transmitted by insects.An emergency sample was sent to the State Key Laboratory of Hygienic Supervision and Inspection at the frontier port of Shanghai Entry-Exit Inspection and Quarantine Bureau at 1am.on 2January 2017.This patient,who was 28 years of age,had returned to Shanghai from Angola.He was diagnosed as being infected with yellow fever virus,and returned to China for further therapy.Using samples of blood and urine,vector-borne pathogens,including Yellow fever virus,Dengue virus,Zika virus,Rift Valley fever virus,Chikungunya virus,West Nile virus,Japanese encephalitis virus,and pathogens from mosquitoes that cause malaria were tested:all were negative.Deep-sequencing data were used to search virus databases,bacteria libraries,and parasite libraries.The whole genome of pathogens was obtained by next-generation sequencing(NGS).The Ekpoma virus was detected by deep sequencing.The whole genome obtained by NGS showed homology of 96% with the Ekpoma virus found in Nigeria.
引文
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