日照口岸首例输入性卵形疟的实验室检测
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摘要
目的调查日照口岸首例输入性卵形疟病例确诊过程,为口岸防控输入性疟疾提供科学依据。方法对1名从非洲回国的劳务人员进行流行病学调查,同时采用胶体金法、血涂片镜检法和荧光PCR法检测疟原虫并分型。结果该病例从利比里亚回国,在境外有疟疾病史,归国后出现发热、寒战等临床症状。实验室快速检测提示为非恶性疟原虫感染;血涂片镜检发现疟原虫滋养体,受染后细胞呈伞矢状;荧光PCR法鉴定为卵形疟原虫感染。结论根据该病例的临床表现和实验室检测结果,判断为输入性卵形疟病例。
Objective To investigate the diagnosis process of the first imported plasmodium. ovale malaria case at Rizhao port,and to provide scientific evidence for the prevention and control of imported malaria cases at ports.Methods A epidemiological investigation was carried out on one labor worker returned from Africa,and colloidal gold method,blood smear microscopy as well as Real-time PCR were adopted to identify the subtype. Results This case came back from Liberia,with a malaria history abroad,and got clinical manifestations including fever,chilling after returned to China. The rapid detection test(RDT) indicated malaria exclusive plasmodium falciparum infection.The smear microscopy found plasmodium trophozoite,the infected cell presented a shape of umbrella sagittal. Realtime PCR showed P. ovale positive. Conclusion It was confirmed as an imported P. ovale malaria case according to the clinical manifestations and laboratory detection.
Objective To investigate the diagnosis process of the first imported plasmodium. ovale malaria case at Rizhao port,and to provide scientific evidence for the prevention and control of imported malaria cases at ports.Methods A epidemiological investigation was carried out on one labor worker returned from Africa,and colloidal gold method,blood smear microscopy as well as Real-time PCR were adopted to identify the subtype. Results This case came back from Liberia,with a malaria history abroad,and got clinical manifestations including fever,chilling after returned to China. The rapid detection test(RDT) indicated malaria exclusive plasmodium falciparum infection.The smear microscopy found plasmodium trophozoite,the infected cell presented a shape of umbrella sagittal. Realtime PCR showed P. ovale positive. Conclusion It was confirmed as an imported P. ovale malaria case according to the clinical manifestations and laboratory detection.
引文
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[7]王加志,尹雪梅,王继琼,等.输入性卵形疟1例[J].中国寄生虫学与寄生虫病杂志,2014,32(1):80.
[8]高世同,李晓恒,黄达娜,等.一宗输入性卵形疟个案调查分析与实验室诊断[J].国际医学寄生中病学杂志,2014,41(6):362-364(368).
[9]李琛琛,尹昆,闫歌.抗疟药青蒿素及其衍生物相关药理作用研究进展[J].中国病原生物学杂志,2016,11(2):185-188.
[2]师永霞,黄吉成,苏锦坤,等.1例国内罕见的输入性卵形疟的实验室检测[J].中国人兽共患病学报,2011,27(10):917.
[3]周瑞敏,张红卫,邓艳,等.2例输入性卵形疟的实验室检测[J].中国寄生虫学与寄生虫病杂志,2013,31(2):127-130.
[4]张孝和,倪庆翔,陈袆,等.温州首例输入性卵形疟病例的诊断分析[J].中国寄生虫学与寄生虫病杂志,2013,31(5):380-381.
[5]王晓光,雷永良,兰进权,等.浙江省1例输入性卵形疟的实验室检测分析[J].中国寄生虫学与寄生虫病杂志,2013,31(1):78-79.
[6]肖婷,魏艳彬,刘凡,等.山东省2例输入性卵形疟病例报告[J].中国病原生物学杂志,2012,7(6):附页4.
[7]王加志,尹雪梅,王继琼,等.输入性卵形疟1例[J].中国寄生虫学与寄生虫病杂志,2014,32(1):80.
[8]高世同,李晓恒,黄达娜,等.一宗输入性卵形疟个案调查分析与实验室诊断[J].国际医学寄生中病学杂志,2014,41(6):362-364(368).
[9]李琛琛,尹昆,闫歌.抗疟药青蒿素及其衍生物相关药理作用研究进展[J].中国病原生物学杂志,2016,11(2):185-188.