叶酸溶液的二维可见-近红外透射光谱响应研究
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摘要
叶酸是一种重要的维生素,在日常饮食中人体摄入叶酸普遍不足。探索叶酸的快速检测手段,对富含叶酸食品的检测及其功能性产品的开发有重要的意义。该研究配置了20个浓度梯度的叶酸溶液,采用可见-近红外光谱仪获得了不同浓度下的透射光谱图。视浓度为外扰,应用二维相关光谱相关理论对叶酸溶液进行分析。结果表明,在可见光谱区内有3个较强的特征波,从强到弱依次为430、450和550 nm,并且在这些波段处的吸光度值与叶酸溶液的浓度呈线性相关,其相关系数在0. 98以上;在短波近红外光谱区内有6个较明显的特征波,从强到弱依次为1 410、1 440、1 490、1 540、1 520和1 510 nm。该研究明确了在可见-近红外透射光谱作用下,叶酸溶液的敏感波段和敏感程度,为叶酸的快速检测提供了理论依据。
Folic acid is an important vitamin in human body. In dialy diet,intaking quantity of folic acid is not enough. Exploring method of rapid detection folic acid is of great significance for detecting raw materials of food and developing functional products rich in folic acid. In this research,folic acid solution with 20 concentration gradients was prepared. Transmission spectrum were acquired by visible-near infrared spectrometer. Folic acid solution was analyzed by two-dimensional correlation spectrum theory and the concentration was regarded as external disturbance. Results showed that there were three characteristic spectrums,from strong to weak,430,450 and 550 nm in visible region. Absorbance values at these bands were linearly dependent on the concentration of folic acid solution,and correlation coefficients were above 0. 98. There were six characteristic spectrums,from strong to weak,1 410,1 440,1 490,1 540,1 520 and 1 510 nm in short wave near infrared spectrum region. In this study,sensitive band and sensitive degree of folic acid solution were pointed out under action of visible-near infrared transmission spectrum. It will be helpful for rapid detection of folic acid.
Folic acid is an important vitamin in human body. In dialy diet,intaking quantity of folic acid is not enough. Exploring method of rapid detection folic acid is of great significance for detecting raw materials of food and developing functional products rich in folic acid. In this research,folic acid solution with 20 concentration gradients was prepared. Transmission spectrum were acquired by visible-near infrared spectrometer. Folic acid solution was analyzed by two-dimensional correlation spectrum theory and the concentration was regarded as external disturbance. Results showed that there were three characteristic spectrums,from strong to weak,430,450 and 550 nm in visible region. Absorbance values at these bands were linearly dependent on the concentration of folic acid solution,and correlation coefficients were above 0. 98. There were six characteristic spectrums,from strong to weak,1 410,1 440,1 490,1 540,1 520 and 1 510 nm in short wave near infrared spectrum region. In this study,sensitive band and sensitive degree of folic acid solution were pointed out under action of visible-near infrared transmission spectrum. It will be helpful for rapid detection of folic acid.
引文
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[15]罗雪宁,倪明航,孔维楠,等.二维相关红外光谱技术对不同冷藏温度的南疆骏枣品质分析研究[J].现代食品科技,2016(2):171-176.
[16]黄冬兰,陈小康.二维相关红外光谱技术快速鉴别三七及其伪品[J].光散射学报,2017,29(1):79-84.HUANG Donglan,CHEN Xiaokang.Study on the identification of standard and false panax notoginseng by two-dimensional infrared correlation spectroscopy[J].The Journal of Light Scattering,2017,29(1):79-84.
[17]宋海燕,程旭.水分对土壤近红外光谱检测影响的二维相关光谱解析[J].光谱学与光谱分析,2014(5):1 240-1 243.SONG Haiyan,CHENG Xu.Analysis of the effect of moisture on soil spectra detection by using two-dimensional correlation near infrared spectroscopy[J].Spectroscopy and Spectral Analysis,2014(5):1 240-1 243.
[18]SIESLER H W,OZAKI Y,KAWATA S,et al.Near-infrared spectroscopy,principles,instruments,applications[M].Germany:wiley-VCH,2002.
[19]NODA I.Progress in two-dimensional(2D)correlation spectroscopy[J].Journal of Molecular Structure,2006,799(1/2/3):2-15.
[2]赵金支,田营彬,赵丹,等.叶酸与神经管畸形相关研究进展[J].生命的化学,2018(2):179-184.
[3]邵丽华.山西省小米叶酸含量的研究[D].临汾:山西师范大学,2014.SHAO Lihua.Study of folic acid content in millet of Shanxi province[D].Linfen:Shanxi Normal University,2014.
[4]梁业红.过量表达细菌的Fol C和Fol P基因对提高拟南芥叶酸含量的研究[D].北京:中国农业科学院,2005.
[5]刘晓宁.过量表达PTR1和GTPCHI基因对提高植物叶酸含量的研究[D].兰州:兰州大学,2010.
[6]黄宏南,陈宏靖,黄健文.高效液相色谱荧光快速测定保健食品中的叶酸[J].中国卫生检验杂志,2002,12(1):70.
[7]DOHERTY R F,BEECHER G R.A method for the analysis of natural and synthetic folate in foods[J].J Agric Food Chem 2003,51(2):354-361.
[8]KLACZKOW G,ANUSZEWSKA E.The use of HPLC method for the folic acid in multicomponent vitamin preparations[J].Acta Pol Pham,2000,57(4):257-260.
[9]文小青,杨月欣.食物中VA、叶酸和VE分析方法研究进展[J].中国食品卫生杂志,2004,16(1):67-70.
[10]汪锦邦,顾鹏,章德宏,等.叶酸分析方法的研究进展[J].中国食品添加剂,2000(3):49-54.
[11]商迎辉,刘馨君,黄汉昌,等.婴幼儿乳品中叶酸的微生物测定方法[J].食品生物技术学报,2018,37(7):727-731.SHANG Yinghui,LIU Xinjun,HUANG Hanchang,et al.Studies of microbiological assay folic acid in infant milk[J].Journal of Food Science and Biotechnology,2018,37(7):727-731.
[12]李娜,王一村,王萌,等.奶粉中维生素B12及叶酸的检测方法对比[J].中国标准化,2018(5):120-126.LI Na,WNAG Yicun,WANG Meng,et al.Comparative study on the testing methods of vitamin B12 and folic acidin milk powder[J].China Standardization,2018(5):120-126.
[13]GIANGIACOMO R.Study of water-sugar interactions at increasing sugar concentration by nir spectroscopy[J].Food Chemistry,2006,96(1):371-379.
[14]NODA I,OZAKI Y.Two-dimensional correlation spectroscopy-applications in vibrational and optical spectroscopy[M].Chichester:West Sussex,England:John Wiley and Sons,Ltd,2004.
[15]罗雪宁,倪明航,孔维楠,等.二维相关红外光谱技术对不同冷藏温度的南疆骏枣品质分析研究[J].现代食品科技,2016(2):171-176.
[16]黄冬兰,陈小康.二维相关红外光谱技术快速鉴别三七及其伪品[J].光散射学报,2017,29(1):79-84.HUANG Donglan,CHEN Xiaokang.Study on the identification of standard and false panax notoginseng by two-dimensional infrared correlation spectroscopy[J].The Journal of Light Scattering,2017,29(1):79-84.
[17]宋海燕,程旭.水分对土壤近红外光谱检测影响的二维相关光谱解析[J].光谱学与光谱分析,2014(5):1 240-1 243.SONG Haiyan,CHENG Xu.Analysis of the effect of moisture on soil spectra detection by using two-dimensional correlation near infrared spectroscopy[J].Spectroscopy and Spectral Analysis,2014(5):1 240-1 243.
[18]SIESLER H W,OZAKI Y,KAWATA S,et al.Near-infrared spectroscopy,principles,instruments,applications[M].Germany:wiley-VCH,2002.
[19]NODA I.Progress in two-dimensional(2D)correlation spectroscopy[J].Journal of Molecular Structure,2006,799(1/2/3):2-15.